Background : This study examined the ameliorative effect of D-3-O-methylchiroinositol, isolated from the stem bark of Piliostigma thonningii, on cadmium chloride-induced osteoporosis in male Wistar rats. Methods : Thi...Background : This study examined the ameliorative effect of D-3-O-methylchiroinositol, isolated from the stem bark of Piliostigma thonningii, on cadmium chloride-induced osteoporosis in male Wistar rats. Methods : Thirty-six rats were assigned to three treatment groups(n = 12). Group A(2 mL distilled water), group B:(2.5 mg/kg b.w. CdCl_2) and group C:(2.5 mg/kg b.w. CdCl_2 and D-3-O-methyl-chiroinositol 2 mg/kg b.w.). Bone ash, calcium, phosphate, magnesium, and zinc content, as well as bone histological changes were determined at the end of months 1, 2, and 3. Results : There were significant differences( P ≤ 0.05) in the weight of the cervical, tibia, and femoral bones in all groups. The serum concentration of CdCl_2 was significantly different across the three groups with time. There was significant variation( P < 0.005) in the mean bone ash across groups. The concentration of OH-proline was significantly different( P < 0.0001) across groups. There were significant differences( P < 0.0001) in bone calcium, magnesium, zinc, and phosphorus concentrations. Histology revealed high levels of bone mineralisation in the CdCl_2-treated group, indicative of osteoporosis with hypertrophied osteocytes, while the femur of Wistar rats treated with D-3-O-methyl-chiroinositol showed bone trabeculae and viable osteocytes. Conclusion : The study concluded that D-3-O-methyl-chiroinositol extract from Piliostigma thionningii stem bark ameliorated cadmium chloride-induced osteoporosis in male Wistar rats.展开更多
Over-production of nitric oxide is pathogenic for neuronal apoptosis around the ischemic area following ischemic brain injury.In this study,an apoptotic model in rat hippocampal neurons was established by 0.5 mmol/L 3...Over-production of nitric oxide is pathogenic for neuronal apoptosis around the ischemic area following ischemic brain injury.In this study,an apoptotic model in rat hippocampal neurons was established by 0.5 mmol/L 3-morpholinosyndnomine(SIN-1),a nitric oxide donor.The models were then cultured with 0.1 mmol/L of 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid(DIDS;the chloride channel blocker) for 18 hours.Neuronal survival was detected using the 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay,and apoptosis was assayed by Hoechst 33342-labeled neuronal DNA fluorescence staining.Western blot analysis and immunochemiluminescence staining were applied to determine the changes of activated caspase-3 and CIC-3 channel proteins.Real-time PCR was used to detect the mRNA expression of CIC-3.The results showed that SIN-1 reduced the neuronal survival rate,induced neuronal apoptosis,and promoted ClC-3 chloride channel protein and mRNA expression in the apoptotic neurons.DIDS reversed the effect of SIN-1.Our findings indicate that the increased activities of the ClC-3 chloride channel may be involved in hippocampal neuronal apoptosis induced by nitric oxide.展开更多
2,2'-Arylmethylene bis(3-hydroxy-5,5-dimethyl-2-cycIohexene-l-one) 41-s produced from reaction between dimedone with various aldehydes in acetonitrile using ZnO as a catalyst; whereas in the presence of ZnO-acetyl...2,2'-Arylmethylene bis(3-hydroxy-5,5-dimethyl-2-cycIohexene-l-one) 41-s produced from reaction between dimedone with various aldehydes in acetonitrile using ZnO as a catalyst; whereas in the presence of ZnO-acetyl chloride catalysts the reaction is limited to give only 1,8-dioxo-octahydroxanthenes 3a-k in very good yields.展开更多
Silica-supported tin chloride and titanium tetrachloride were prepared by the reaction of tin chloride and titanium tetrachloride with activated silica gel in refluxing toluene.These solid acids have been employed as ...Silica-supported tin chloride and titanium tetrachloride were prepared by the reaction of tin chloride and titanium tetrachloride with activated silica gel in refluxing toluene.These solid acids have been employed as the catalysts for the synthesis of bisdihydropyrimidin -2(1H)-ones from aromatic dialdehydes,1,3-dicarbonyl compounds and urea at 90℃under solvent-free conditions.展开更多
In the present study,Fmr1 knockout mice (KO mice) were used as the model for fragile X syndrome.The results of step-through and step-down tests demonstrated that Fmr1 KO mice had shorter latencies and more error count...In the present study,Fmr1 knockout mice (KO mice) were used as the model for fragile X syndrome.The results of step-through and step-down tests demonstrated that Fmr1 KO mice had shorter latencies and more error counts,indicating a learning and memory disorder.After treatment with 30,60,90,120,or 200 mg/kg lithium chloride,the learning and memory abilities of the Fmr1 KO mice were significantly ameliorated,in particular,the 200 mg/kg lithium chloride treatment had the most significant effect.Western blot analysis showed that lithium chloride significantly enhanced the expression of phosphorylated glycogen synthase kinase 3 beta,an inactive form of glycogen synthase kinase 3 beta,in the cerebral cortex and hippocampus of the Fmr1 KO mice.These results indicated that lithium chloride improved learning and memory in the Fmr1 KO mice,possibly by inhibiting glycogen synthase kinase 3 beta activity.展开更多
In this study, we used a mixture of organic liquid propane-1,3-diol and ionic liquid 1-ethyl-3-methylimidazolium chloride([emim]Cl) as the entrainer to separate tert-butyl alcohol(TBA) + water. We measured the isobari...In this study, we used a mixture of organic liquid propane-1,3-diol and ionic liquid 1-ethyl-3-methylimidazolium chloride([emim]Cl) as the entrainer to separate tert-butyl alcohol(TBA) + water. We measured the isobaric vapor–liquid equilibrium(VLE) for the quaternary system TBA + water + propane-1,3-diol + [emim]Cl at 101.3 kPa, and found the VLE data to be well correlated with the nonrandom two-liquid model. These results show that the mixed solvent of propane-1,3-diol + [emim]Cl can increase the relative volatility of TBA to water and break the azeotropic point. We found no notable synergetic effect between them, and observed that the liquid mixed solvent of propane-1,3-diol and [emim]Cl had lower viscosity than [emim]Cl, which makes it a promising entrainer for separating the TBA + water azeotrope in industrial applications.展开更多
BACKGROUND: Previous studies have demonstrated that scorpion venom in the scorpion can inhibit epilepsy and apoptosis.However,it remains unclear whether ethanol extracts of scorpion (EES) exhibit similar effects.OBJEC...BACKGROUND: Previous studies have demonstrated that scorpion venom in the scorpion can inhibit epilepsy and apoptosis.However,it remains unclear whether ethanol extracts of scorpion (EES) exhibit similar effects.OBJECTIVE: To investigate the effects of EES on hippocampal apoptosis and caspase-3 expression,and to compare the effects on sodium valproate (positive control drug) in a rat model of status epilepticus induced by lithium chloride-pilocarpine.DESIGN,TIME AND SETTING: This randomized,controlled study was conducted at the Drug Research and Development Center,Kanghong Pharmaceuticals Group,and the Department of Pathology,Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital,China from May 2007 to April 2008.MATERIALS: EES were prepared by Huashen Pharmaceutical,China.Sodium valproate (Hunan Xiangzhong Pharmaceutical,China) and lithium chloride-pilocarpine (Sigma,USA) were also used in the present study.METHODS: From a total of 156 rats,six served as normal controls.The remaining rats were intraperitoneally injected with lithium chloride-pilocarpine to establish status epilepticus models,and then assigned to five groups (n = 30,respectively).Animals in each group were administered drugs at 15 minutes after epileptic seizure by gavage.i.e.in the normal control and model groups,rats were treated with 1 mL/0.1 kg saline.The sodium valproate group was administered 120 mg/kg/d sodium valproate.The low-,moderate-,and high-dose EES groups received treatments of 290,580,and 1 160 mg/kg/d EES.The dispensed concentration was 1 mL/0.1 kg.Rat seizure behavior was observed.If status epilepticus did not terminated after 1 hour,the rats were intraperitoneally administered atropine (1 mg/kg) and diazepam (10 mg/kg) to terminate seizure.These rats were continuously observed for 6 hours to ensure seizure termination.Then rats were treated with the above-mentioned drugs at 8: 00 am each day until sacrifice,which took place 4 hours after drug administration.MAIN OUTCOME MEASURES: Terminal dUTP nick end labeling (TUNEL)-positive cells and caspase-3 expression were,respectively,determined by TUNEL and immunohistochemistry at 6,24,48,and 72 hours,as well as 7 days,after status epilepticus.Behavioral changes were also measured.RESULTS: A few caspase-3-positive cells were observed.TUNEL-and caspase-3-positive cells were mainly visible in the hippocampal CA1 and CA3 regions 6 hours following status epilepticus in the model and drug intervention groups.The number of TUNEL-positive cells reached a peak at 48 hours following status epilepticus in the sodium valproate group,as well as the moderate-and high-dose EES groups,and number of TUNEL-positive cells reached a peak at 72 hours in the model and low-dose EES groups.The number of caspase-3-positive cells reached a peak at 48 hours in each group.Following treatment of sodium valproate and EES,the number of TUNEL-and caspase-3-positive cells significantly decreased compared with the model group at various time points (P < 0.05).The number of TUNEL-and caspase-3-positive cells was greatest in the low-dose EES group,followed by the moderate-and high-dose EES groups.The number of TUNEL-and caspase-3-positive cells was similar between the sodium valproate and high-dose EES groups.Epileptic seizure was significantly improved in the sodium valproate group,as well as the moderate-and high-dose EES groups,compared with the model group (P < 0.05 or P < 0.01).Treatment with sodium valproate and high-dose EES resulted in the best outcome,although the results were similar (P > 0.05).CONCLUSION: A dose of 1 160 mg/kg/d EES significantly inhibited status epilepticus.This outcome corresponded to a decreased number of apoptotic cells and caspase-3-positive cells,which was similar to sodium valproate.These results suggest that it is not necessary to extract a component from the scorpion for the treatment of epilepsy.The high dose of EES significantly inhibited epilepsy,which correlated with decreased hippocampal caspase-3 expression.展开更多
We previously demonstrated that 2-hydroxypropyltrimethyl ammonium chloride chitosan(HACC)promoted the production of nitric oxide(NO)and proinflammatory cytokines by activating the mitogen-activated protein kinases(MAP...We previously demonstrated that 2-hydroxypropyltrimethyl ammonium chloride chitosan(HACC)promoted the production of nitric oxide(NO)and proinflammatory cytokines by activating the mitogen-activated protein kinases(MAPK)and Janus kinase(JAK)/STAT pathways in RAW 264.7 cells,indicating good immunomodulatory activity of HACC.In this study,to further investigate the immunomodulatory mechanisms of HACC,we determined the roles of phosphatidylinositol 3-kinase(PI3K)/Akt,activating protein(AP-1)and nuclear factor kappa B(NF-κB)in HACC-induced activation of RAW 264.7 cells by the western blotting.The results suggest that HACC promoted the phosphorylation of p85 and Akt.Furthermore,c-Jun and p65 were also increased after the treatment of RAW 264.7 cells with HACC,indicating the translocation of NF-κB and AP-1 from cytoplasm to nucleus.In addition,as scanning electron microscopy(SEM)analysis shows,the cell morphology changed after HACC treatment.These findings indicate that HACC activated MAPK,JAK/STAT,and PI3K/Akt signaling pathways dependent on AP-1 and NF-κB activation in RAW 264.7 cells,ultimately leading to the increase of NO and cytokines.展开更多
Use of free air as oxidant and copper(II)chloride as catalyst,3,3'-di-tert-butylbi-phenyl-2,5,2',5'-diquinone(BBDQ)was prepared via catalytic oxidation of 2,2'-dihydroxy-3,3'-di-tert-butyl-5,5'...Use of free air as oxidant and copper(II)chloride as catalyst,3,3'-di-tert-butylbi-phenyl-2,5,2',5'-diquinone(BBDQ)was prepared via catalytic oxidation of 2,2'-dihydroxy-3,3'-di-tert-butyl-5,5'-dimethoxy-biphenyl(di-BHA).The yield of reaction attained 95% and the selectivity for BBDQ was 100%.The single-crystal X-ray diffraction analysis reveals that the dihedral angle between two rings(benzene rings for di-BHA and quinone rings for BBDQ)changes from 89.8 to 45.3o,indicating the steric hindrance effects of methyl disappear in the oxidation process.The crystal structures of di-BHA and BBDQ are further confirmed by their spectral characterizations.The probable mechanism for this oxidation process is also discussed.展开更多
Poly[2,2-(m-phenylene)-5,5-bibenzimidazole](mPBI)were synthesized by mixing 3,3',4,4'-tetraaminobiphenyl and isophthalic acid in 1-butyl-3-methylimidazolium chloride([BMIM]Cl).Intrinsic viscosity of mPBI polym...Poly[2,2-(m-phenylene)-5,5-bibenzimidazole](mPBI)were synthesized by mixing 3,3',4,4'-tetraaminobiphenyl and isophthalic acid in 1-butyl-3-methylimidazolium chloride([BMIM]Cl).Intrinsic viscosity of mPBI polymers was 0.67 dL/g which was measured in 96%sulfuric acid.The polymer was characterized by Fourier transform infrared spectroscopy(FTIR),nuclear magnetic resonance(1H-NMR),and thermogravimetric analysis(TGA).The effects of polymerization conditions on the intrinsic viscosity of mPBI were investigated.It showed that the molecular weight of polymer mainly depended on pre-reaction time and reaction temperature.Comparison of structure and properties of mPBI synthesized in ionic liquids(ILs)and polyphosphoric acid was also reported.It indicates that the ionic liquids are a kind of good solvents in synthesis process of mPBI and ionic liquids mainly affect molecular weight of mPBI.展开更多
Objective To analyze the relationship between malignant transformation and abnormal expression of eukaryotic initiation factor 3 (eIF3 p36) in human bronchial epithelial (16HBE) cells induced by cadmium chloride (CdCl...Objective To analyze the relationship between malignant transformation and abnormal expression of eukaryotic initiation factor 3 (eIF3 p36) in human bronchial epithelial (16HBE) cells induced by cadmium chloride (CdCl2). Methods 16HBE cells were treated several times with different concentrations of CdCl2. Tumorigenic potential of transformed cells was identified by assays for anchorage-independent growth in soft agar and for tumorigenicity in nude mice after the 35th passage. Total RNA was isolated from 16HBE cells induced by CdCl2, including non-transformed, Cd-transformed, and Cd-tumorigenic cell lines. Special primers for eIF3 p36 were designed and the expression of eIF3 mRNA in different cell lines was detected with fluorescent quantitative-polymerase chain reaction technique (FQ-PCR). Results The 35th passage of 16HBE cells transformed by CdCl2 exhibited overlapping growth. Compared with the non-transformed cells, colonies of transformed cell lines in soft agar showed statistically significant increases and dose-dependent effects (P<0.01). All Cd-induced transformed cell lines formed tumors in nude mice within 2 weeks of inoculation, but none of the mice injected with non-transformed cells showed tumors even after 3 weeks. All tumors were pathologically identified as poorly differentiated squamous cell carcinoma. The eIF3 p36 genes in different stages of 16HBE cells transformed by CdCl2 were elevated as compared with the non-transformed control (P<0.01), and the eIF3 expression increased with the degree of cell malignancy. Conclusion CdCl2 is capable of inducing morphological transformation in 16HBE cells and transformed cells are potentially tumorigenic. Over-expression of eIF3 p36 is positively correlated with malignant transformation of 16HBE cells induced by CdCl2 and may be one of the molecular mechanisms potentially responsible for carcinogenesis due to Cd.展开更多
Objective: To investigate the role of ClC-3 chloride channel in the proliferation of breast cancer cell line Mcf-7 treated with curcumin and its specific mechanism. Methods: MTT assay was used to detect the effect of ...Objective: To investigate the role of ClC-3 chloride channel in the proliferation of breast cancer cell line Mcf-7 treated with curcumin and its specific mechanism. Methods: MTT assay was used to detect the effect of chloride channel blocker(DIDS) and curcumin on Mcf-7 and human normal cell viability. Patch-clamp technique was used to determine the current density before and after drug treatment. Apoptosis assay by flow cytometry was performed for further examination of cell apoptosis. Results: Curcumin had toxicity on Mcf-7 and HUVEC cells and DIDS reduced the survival rate of Mcf-7 cells by inhibiting proliferation. Curcumin could activate the chloride ion current on MCF-7 cell membrane, which would be inhibited by DIDS.Finally, curcumin in low concentration combined with DIDS could significantly promote the MCF-7 cells apoptosis. Conclusions: Our results suggest that ClC-3 protein is involved in the regulation of curcumin induced proliferation inhibiting in breast cancer cells through inducing cell apoptosis. ClC-3 may be a potential target of tumor therapy.展开更多
文摘Background : This study examined the ameliorative effect of D-3-O-methylchiroinositol, isolated from the stem bark of Piliostigma thonningii, on cadmium chloride-induced osteoporosis in male Wistar rats. Methods : Thirty-six rats were assigned to three treatment groups(n = 12). Group A(2 mL distilled water), group B:(2.5 mg/kg b.w. CdCl_2) and group C:(2.5 mg/kg b.w. CdCl_2 and D-3-O-methyl-chiroinositol 2 mg/kg b.w.). Bone ash, calcium, phosphate, magnesium, and zinc content, as well as bone histological changes were determined at the end of months 1, 2, and 3. Results : There were significant differences( P ≤ 0.05) in the weight of the cervical, tibia, and femoral bones in all groups. The serum concentration of CdCl_2 was significantly different across the three groups with time. There was significant variation( P < 0.005) in the mean bone ash across groups. The concentration of OH-proline was significantly different( P < 0.0001) across groups. There were significant differences( P < 0.0001) in bone calcium, magnesium, zinc, and phosphorus concentrations. Histology revealed high levels of bone mineralisation in the CdCl_2-treated group, indicative of osteoporosis with hypertrophied osteocytes, while the femur of Wistar rats treated with D-3-O-methyl-chiroinositol showed bone trabeculae and viable osteocytes. Conclusion : The study concluded that D-3-O-methyl-chiroinositol extract from Piliostigma thionningii stem bark ameliorated cadmium chloride-induced osteoporosis in male Wistar rats.
基金Supported by the National Natural Science Foundation of China(21076176) the Research and Development Project of Tangshan(10140201C-3)+1 种基金 the Research and Development Project of Hebei Province(07275113) the Research Fund of Tangshan Normal College
基金supported by the National Natural Science Foundation of China,No.81160157a grant from Guizhou Science and Technology Department in China,No.SY20093075Technological Talents Funds of Guizhou Province in China,No.201209
文摘Over-production of nitric oxide is pathogenic for neuronal apoptosis around the ischemic area following ischemic brain injury.In this study,an apoptotic model in rat hippocampal neurons was established by 0.5 mmol/L 3-morpholinosyndnomine(SIN-1),a nitric oxide donor.The models were then cultured with 0.1 mmol/L of 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid(DIDS;the chloride channel blocker) for 18 hours.Neuronal survival was detected using the 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay,and apoptosis was assayed by Hoechst 33342-labeled neuronal DNA fluorescence staining.Western blot analysis and immunochemiluminescence staining were applied to determine the changes of activated caspase-3 and CIC-3 channel proteins.Real-time PCR was used to detect the mRNA expression of CIC-3.The results showed that SIN-1 reduced the neuronal survival rate,induced neuronal apoptosis,and promoted ClC-3 chloride channel protein and mRNA expression in the apoptotic neurons.DIDS reversed the effect of SIN-1.Our findings indicate that the increased activities of the ClC-3 chloride channel may be involved in hippocampal neuronal apoptosis induced by nitric oxide.
基金support from the Research Council of University of Sistan and Baluchestan, Iran
文摘2,2'-Arylmethylene bis(3-hydroxy-5,5-dimethyl-2-cycIohexene-l-one) 41-s produced from reaction between dimedone with various aldehydes in acetonitrile using ZnO as a catalyst; whereas in the presence of ZnO-acetyl chloride catalysts the reaction is limited to give only 1,8-dioxo-octahydroxanthenes 3a-k in very good yields.
文摘Silica-supported tin chloride and titanium tetrachloride were prepared by the reaction of tin chloride and titanium tetrachloride with activated silica gel in refluxing toluene.These solid acids have been employed as the catalysts for the synthesis of bisdihydropyrimidin -2(1H)-ones from aromatic dialdehydes,1,3-dicarbonyl compounds and urea at 90℃under solvent-free conditions.
基金the National Natural Science Foundation of China,No.30870876the Natural Science Foundation of Guangdong Province,No.815101700100005+2 种基金the Science and Technology Program of Guangdong Province,No.2005B60302004,2008B030301371,2009B030801368the Traditional Chinese Medicineand Combination of Traditional Chinese and Western Medicine Program of Guangzhou,No.2008A52the Medical and Health Scientific Research Program of Guangzhou,No.2009-YB-167
文摘In the present study,Fmr1 knockout mice (KO mice) were used as the model for fragile X syndrome.The results of step-through and step-down tests demonstrated that Fmr1 KO mice had shorter latencies and more error counts,indicating a learning and memory disorder.After treatment with 30,60,90,120,or 200 mg/kg lithium chloride,the learning and memory abilities of the Fmr1 KO mice were significantly ameliorated,in particular,the 200 mg/kg lithium chloride treatment had the most significant effect.Western blot analysis showed that lithium chloride significantly enhanced the expression of phosphorylated glycogen synthase kinase 3 beta,an inactive form of glycogen synthase kinase 3 beta,in the cerebral cortex and hippocampus of the Fmr1 KO mice.These results indicated that lithium chloride improved learning and memory in the Fmr1 KO mice,possibly by inhibiting glycogen synthase kinase 3 beta activity.
基金supported by the Innovation Fund of Tianjin University (No. 2010XJ-0022)
文摘In this study, we used a mixture of organic liquid propane-1,3-diol and ionic liquid 1-ethyl-3-methylimidazolium chloride([emim]Cl) as the entrainer to separate tert-butyl alcohol(TBA) + water. We measured the isobaric vapor–liquid equilibrium(VLE) for the quaternary system TBA + water + propane-1,3-diol + [emim]Cl at 101.3 kPa, and found the VLE data to be well correlated with the nonrandom two-liquid model. These results show that the mixed solvent of propane-1,3-diol + [emim]Cl can increase the relative volatility of TBA to water and break the azeotropic point. We found no notable synergetic effect between them, and observed that the liquid mixed solvent of propane-1,3-diol and [emim]Cl had lower viscosity than [emim]Cl, which makes it a promising entrainer for separating the TBA + water azeotrope in industrial applications.
基金the National Natural Science Foundation of China,No.30740035the Tackle Key Program of Sichuan Province,No.05SG1672
文摘BACKGROUND: Previous studies have demonstrated that scorpion venom in the scorpion can inhibit epilepsy and apoptosis.However,it remains unclear whether ethanol extracts of scorpion (EES) exhibit similar effects.OBJECTIVE: To investigate the effects of EES on hippocampal apoptosis and caspase-3 expression,and to compare the effects on sodium valproate (positive control drug) in a rat model of status epilepticus induced by lithium chloride-pilocarpine.DESIGN,TIME AND SETTING: This randomized,controlled study was conducted at the Drug Research and Development Center,Kanghong Pharmaceuticals Group,and the Department of Pathology,Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital,China from May 2007 to April 2008.MATERIALS: EES were prepared by Huashen Pharmaceutical,China.Sodium valproate (Hunan Xiangzhong Pharmaceutical,China) and lithium chloride-pilocarpine (Sigma,USA) were also used in the present study.METHODS: From a total of 156 rats,six served as normal controls.The remaining rats were intraperitoneally injected with lithium chloride-pilocarpine to establish status epilepticus models,and then assigned to five groups (n = 30,respectively).Animals in each group were administered drugs at 15 minutes after epileptic seizure by gavage.i.e.in the normal control and model groups,rats were treated with 1 mL/0.1 kg saline.The sodium valproate group was administered 120 mg/kg/d sodium valproate.The low-,moderate-,and high-dose EES groups received treatments of 290,580,and 1 160 mg/kg/d EES.The dispensed concentration was 1 mL/0.1 kg.Rat seizure behavior was observed.If status epilepticus did not terminated after 1 hour,the rats were intraperitoneally administered atropine (1 mg/kg) and diazepam (10 mg/kg) to terminate seizure.These rats were continuously observed for 6 hours to ensure seizure termination.Then rats were treated with the above-mentioned drugs at 8: 00 am each day until sacrifice,which took place 4 hours after drug administration.MAIN OUTCOME MEASURES: Terminal dUTP nick end labeling (TUNEL)-positive cells and caspase-3 expression were,respectively,determined by TUNEL and immunohistochemistry at 6,24,48,and 72 hours,as well as 7 days,after status epilepticus.Behavioral changes were also measured.RESULTS: A few caspase-3-positive cells were observed.TUNEL-and caspase-3-positive cells were mainly visible in the hippocampal CA1 and CA3 regions 6 hours following status epilepticus in the model and drug intervention groups.The number of TUNEL-positive cells reached a peak at 48 hours following status epilepticus in the sodium valproate group,as well as the moderate-and high-dose EES groups,and number of TUNEL-positive cells reached a peak at 72 hours in the model and low-dose EES groups.The number of caspase-3-positive cells reached a peak at 48 hours in each group.Following treatment of sodium valproate and EES,the number of TUNEL-and caspase-3-positive cells significantly decreased compared with the model group at various time points (P < 0.05).The number of TUNEL-and caspase-3-positive cells was greatest in the low-dose EES group,followed by the moderate-and high-dose EES groups.The number of TUNEL-and caspase-3-positive cells was similar between the sodium valproate and high-dose EES groups.Epileptic seizure was significantly improved in the sodium valproate group,as well as the moderate-and high-dose EES groups,compared with the model group (P < 0.05 or P < 0.01).Treatment with sodium valproate and high-dose EES resulted in the best outcome,although the results were similar (P > 0.05).CONCLUSION: A dose of 1 160 mg/kg/d EES significantly inhibited status epilepticus.This outcome corresponded to a decreased number of apoptotic cells and caspase-3-positive cells,which was similar to sodium valproate.These results suggest that it is not necessary to extract a component from the scorpion for the treatment of epilepsy.The high dose of EES significantly inhibited epilepsy,which correlated with decreased hippocampal caspase-3 expression.
基金Supported by the National Key R&D Program of China(No.2018YFC0311305)the Key Research and Development Program of Shandong Province(Nos.2019GHY112015,2019YYSP028)。
文摘We previously demonstrated that 2-hydroxypropyltrimethyl ammonium chloride chitosan(HACC)promoted the production of nitric oxide(NO)and proinflammatory cytokines by activating the mitogen-activated protein kinases(MAPK)and Janus kinase(JAK)/STAT pathways in RAW 264.7 cells,indicating good immunomodulatory activity of HACC.In this study,to further investigate the immunomodulatory mechanisms of HACC,we determined the roles of phosphatidylinositol 3-kinase(PI3K)/Akt,activating protein(AP-1)and nuclear factor kappa B(NF-κB)in HACC-induced activation of RAW 264.7 cells by the western blotting.The results suggest that HACC promoted the phosphorylation of p85 and Akt.Furthermore,c-Jun and p65 were also increased after the treatment of RAW 264.7 cells with HACC,indicating the translocation of NF-κB and AP-1 from cytoplasm to nucleus.In addition,as scanning electron microscopy(SEM)analysis shows,the cell morphology changed after HACC treatment.These findings indicate that HACC activated MAPK,JAK/STAT,and PI3K/Akt signaling pathways dependent on AP-1 and NF-κB activation in RAW 264.7 cells,ultimately leading to the increase of NO and cytokines.
基金supported by the 973 Program (2005CB623607)the National Natural Science Foundation of China (No.20771061)the scientific program 2008BAE64B09
文摘Use of free air as oxidant and copper(II)chloride as catalyst,3,3'-di-tert-butylbi-phenyl-2,5,2',5'-diquinone(BBDQ)was prepared via catalytic oxidation of 2,2'-dihydroxy-3,3'-di-tert-butyl-5,5'-dimethoxy-biphenyl(di-BHA).The yield of reaction attained 95% and the selectivity for BBDQ was 100%.The single-crystal X-ray diffraction analysis reveals that the dihedral angle between two rings(benzene rings for di-BHA and quinone rings for BBDQ)changes from 89.8 to 45.3o,indicating the steric hindrance effects of methyl disappear in the oxidation process.The crystal structures of di-BHA and BBDQ are further confirmed by their spectral characterizations.The probable mechanism for this oxidation process is also discussed.
基金Natural Science Foundation of Shanghai,China(No.09ZR1401500)
文摘Poly[2,2-(m-phenylene)-5,5-bibenzimidazole](mPBI)were synthesized by mixing 3,3',4,4'-tetraaminobiphenyl and isophthalic acid in 1-butyl-3-methylimidazolium chloride([BMIM]Cl).Intrinsic viscosity of mPBI polymers was 0.67 dL/g which was measured in 96%sulfuric acid.The polymer was characterized by Fourier transform infrared spectroscopy(FTIR),nuclear magnetic resonance(1H-NMR),and thermogravimetric analysis(TGA).The effects of polymerization conditions on the intrinsic viscosity of mPBI were investigated.It showed that the molecular weight of polymer mainly depended on pre-reaction time and reaction temperature.Comparison of structure and properties of mPBI synthesized in ionic liquids(ILs)and polyphosphoric acid was also reported.It indicates that the ionic liquids are a kind of good solvents in synthesis process of mPBI and ionic liquids mainly affect molecular weight of mPBI.
基金the National Natural Science Foundation of China (No. 30371195)Guangdong NaturalScience Foundation (No. 06022672)+1 种基金Guangzhou Science and Technology Foundation (No. 2003Z2-E0191/E0192)Guangzhou Municipal Department of Education (No. 1002)
文摘Objective To analyze the relationship between malignant transformation and abnormal expression of eukaryotic initiation factor 3 (eIF3 p36) in human bronchial epithelial (16HBE) cells induced by cadmium chloride (CdCl2). Methods 16HBE cells were treated several times with different concentrations of CdCl2. Tumorigenic potential of transformed cells was identified by assays for anchorage-independent growth in soft agar and for tumorigenicity in nude mice after the 35th passage. Total RNA was isolated from 16HBE cells induced by CdCl2, including non-transformed, Cd-transformed, and Cd-tumorigenic cell lines. Special primers for eIF3 p36 were designed and the expression of eIF3 mRNA in different cell lines was detected with fluorescent quantitative-polymerase chain reaction technique (FQ-PCR). Results The 35th passage of 16HBE cells transformed by CdCl2 exhibited overlapping growth. Compared with the non-transformed cells, colonies of transformed cell lines in soft agar showed statistically significant increases and dose-dependent effects (P<0.01). All Cd-induced transformed cell lines formed tumors in nude mice within 2 weeks of inoculation, but none of the mice injected with non-transformed cells showed tumors even after 3 weeks. All tumors were pathologically identified as poorly differentiated squamous cell carcinoma. The eIF3 p36 genes in different stages of 16HBE cells transformed by CdCl2 were elevated as compared with the non-transformed control (P<0.01), and the eIF3 expression increased with the degree of cell malignancy. Conclusion CdCl2 is capable of inducing morphological transformation in 16HBE cells and transformed cells are potentially tumorigenic. Over-expression of eIF3 p36 is positively correlated with malignant transformation of 16HBE cells induced by CdCl2 and may be one of the molecular mechanisms potentially responsible for carcinogenesis due to Cd.
基金funded by grants from the Science and Technology Planning Projeet of Guangdong Province (2014A020211022)Technology Planning Project of Guangzhou (201510010074.201607010063 and 201607010216)
文摘Objective: To investigate the role of ClC-3 chloride channel in the proliferation of breast cancer cell line Mcf-7 treated with curcumin and its specific mechanism. Methods: MTT assay was used to detect the effect of chloride channel blocker(DIDS) and curcumin on Mcf-7 and human normal cell viability. Patch-clamp technique was used to determine the current density before and after drug treatment. Apoptosis assay by flow cytometry was performed for further examination of cell apoptosis. Results: Curcumin had toxicity on Mcf-7 and HUVEC cells and DIDS reduced the survival rate of Mcf-7 cells by inhibiting proliferation. Curcumin could activate the chloride ion current on MCF-7 cell membrane, which would be inhibited by DIDS.Finally, curcumin in low concentration combined with DIDS could significantly promote the MCF-7 cells apoptosis. Conclusions: Our results suggest that ClC-3 protein is involved in the regulation of curcumin induced proliferation inhibiting in breast cancer cells through inducing cell apoptosis. ClC-3 may be a potential target of tumor therapy.