尿液中1,3-丁二烯代谢产物的液相色谱-串联质谱法检测

建立了人体尿液中1，3-丁二烯的代谢产物——丁烯醇巯基尿酸（MHBMA）和丁烯二醇巯基尿酸（DHBMA）的液相色谱-串联质谱（LC—MS／MS）检测方法。以DHBMA—D7、MHBMA—D6作内标，尿液酸化离心后采用反相固相萃取柱Isolute ENV＋净化和富集。分析样品以无水甲醇和pH2．5的0．1％甲酸水溶液为流动相，经Agilent Extend C18柱梯度洗脱，流速1mL／min，柱后以三通分流，分流比约为3：1，柱温40℃。在多离子监测模式下进行定性和定量分析，采用负离子扫描模式。2种代谢物的精密度为1．02％～7．79％，回收率为97％～112％。用该方法同时测定了吸烟者和非吸烟者尿液中2种代谢物的平均质量浓度，结果表明，对于抽吸相同品牌相同支数的卷烟的吸烟者，由于吸烟行为和代谢途径不同，其尿中DHBMA和MHBMA的质量浓度存在着较大差异，而对于吸烟者尿液，其DHBMA、MHBMA的质量浓度分别是非吸烟者的5．1倍和9．1倍。因此，DHBMA和MHBMA有可能作为人体尿液中鉴别吸烟者和非吸烟者的生物标记物。

Acrolein as a novel therapeutic target for motor and sensory deficits in spinal cord injury

In the hours to weeks following traumatic spinal cord injuries （SCI）, biochemical processes are initiated that further damage the tissue within and surrounding the initial injury site： a process termed secondary injury. Acrolein, a highly reactive unsaturated aldehyde, has been shown to play a major role in the secondary injury by contributing significantly to both motor and sensory defi- cits. In particular, efforts have been made to eluddate the mechanisms of acrolein-mediated dam- age at the cellular level and the resulting paralysis and neuropathic pain. In this review, we will highlight the recent developments in the understanding of the mechanisms of acrolein in motor and sensory dysfunction in animal models of SCI. We will also discuss the therapeutic benefits of using acrolein scavengers to attenuate acrolein-mediated neuronal damage following SCI.