Investigation on mechanical properties regulation of rock-like specimens based on 3D printing and similarity quantification

3D printing is widely adopted to quickly produce rock mass models with complex structures in batches,improving the consistency and repeatability of physical modeling.It is necessary to regulate the mechanical properties of 3D-printed specimens to make them proportionally similar to natural rocks.This study investigates mechanical properties of 3D-printed rock analogues prepared by furan resin-bonded silica sand particles.The mechanical property regulation of 3D-printed specimens is realized through quantifying its similarity to sandstone,so that analogous deformation characteristics and failure mode are acquired.Considering similarity conversion,uniaxial compressive strength,cohesion and stress–strain relationship curve of 3D-printed specimen are similar to those of sandstone.In the study ranges,the strength of 3D-printed specimen is positively correlated with the additive content,negatively correlated with the sand particle size,and first increases then decreases with the increase of curing temperature.The regulation scheme with optimal similarity quantification index,that is the sand type of 70/140,additive content of 2.5‰and curing temperature of 81.6℃,is determined for preparing 3D-printed sandstone analogues and models.The effectiveness of mechanical property regulation is proved through uniaxial compression contrast tests.This study provides a reference for preparing rock-like specimens and engineering models using 3D printing technology.

Investigating the Genetic Bases of Growth Regulation by E2F3 in Dwarf Surf Clams Mulinia lateralis

Bivalve aquaculture plays a crucial role in the aquaculture industry due to the economic value of many bivalve species.Understanding the underlying genetic basis of bivalve growth regulation is essential for enhancing germplasm innovation and ensuring sustainable development of the industry.Though numerous candidate genes have been identified,their functional validation remains challenging.Fortunately,the dwarf surf clam(Mulinia lateralis)serves as a promising model organism for investigating genetic mechanisms underlying growth regulation in bivalves.The GWAS study in the Yesso scallop(Patinopecten yessoensis)has pinpointed the E2F3 gene as a key regulator of growth-related traits.However,the specific role of E2F3 in bivalve growth remains unclear.This study aimed to further confirm the regulatory function of the E2F3 gene in the dwarf surf clam through RNA interference experiments.Our results revealed several genes are associated with individual growth and development,including CTS7,HSP70B2,and PGLYRP3,as well as genes involved in lipid metabolism such as FABP2 and FASN.Functional enrichment analysis indicated that E2F3 primarily modulates critical processes like amino acid and lipid metabolism.These findings suggest that E2F3 likely regulates growth in the dwarf surf clam by influencing amino acid and lipid metabolism.Overall,this study advances our understanding on the function of E2F3 gene in growth regulation in bivalves,providing valuable insights for future research in this field.

Exploring the relationship between red blood cell levels and emotional regulation through the miR191-Riok3-Mxi1 pathway

Objective:To assess emotional fluctuations,physical and mental health status,and indicators closely related to red blood cells,such as RIO kinase 3(Riok3),MAX interactor 1(Mxi1),and microRNA 191(miR191),in participants with different levels of red blood cells.Methods:Participants who underwent physical examinations at Dongfang Hospital between April and October 2019 were divided into healthy,blood deficiency,and anemia groups(30 individuals in the healthy and blood deficiency group respectively,and 13 in the anemia group).The physical and mental conditions of the participants were evaluated through questionnaires,and emotional fluctuations were assessed through an emotion-inducing experiment,in which participants watched video segments designed to induce specific emotions.Relative expression levels of miR191,Riok3,and Mxi1 from venous blood samples were also determined.Results:The main psychological factors identified in the anemia and blood deficiency groups were obsessive-compulsive symptoms,depression,anxiety,and other negative emotions.Relative gene expression levels indicated that miR191 was upregulated and Riok3 and Mxi1 were downregulated in both the blood deficiency and anemia groups.Regarding the emotional score of disgust on video stage,the main effect was significant(F=335.58,P<.001),which showed that watching the three videos caused participants to have a dominant emotion,and there is a difference on group(F=5.35,P=.01),with higher disgust scores in the anemia and blood deficiency groups.The symptoms of blood deficiency and anemia,such as weakness in limbs were significantly negatively correlated with Riok3 and Mxi1 expression(r=-0.38 and-0.31 respectively),but was significantly positively correlated with miR191 expression(r=0.29).Conclusion:We determined that a close relationship exists between red blood cell levels and emotional status.Our findings suggest that individuals with anemia and blood deficiency are more likely to experience psychological problems and negative emotions,particularly disgust.We also demonstrate that emotional regulation is related to mir191-Riok3-Mxi1 pathway activity,identifying these pathway components are potential targets for genetic therapies in combination with psychological therapy.

Negative feedback regulation of cellular antiviral signaling by RBCKl-mediated degradation of IRF3

Viral infection causes host cells to produce type I interferons （IFNs）, which are critically involved in viral clearance. Previous studies have demonstrated that activation of the transcription factor interferon regulatory factor （IRF）3 is essential for virus-triggered induction of type I IFNs. Here we show that the E3 ubiquitin ligase RBCC protein interacting with PKC1 （RBCK1） catalyzes the ubiquitination and degradation of IRF3. Overexpression of RBCK1 negatively regulates Sendai virus-triggered induction of type I IFNs, while knockdown of RBCK1 has the opposite effect. Plaque assays consistently demonstrate that RBCKI negatively regulates the cellular antiviral response. Furthermore, viral infection leads to induction of RBCK1 and subsequent degradation of IRF3. These findings suggest that the cellular antiviral response is controlled by a negative feedback regulatory mechanism involving RBCKl-mediated ubiquitination and degradation of IRF3.

Balancing the customization and standardization:exploration and layout surrounding the regulation of the growing field of 3D-printed medical devices in China

Medical devices are instruments and other tools that act on the human body to aid clinical diagnosis and disease treatment,playing an indispensable role in modern medicine.Nowadays,the increasing demand for personalized medical devices poses a significant challenge to traditional manufacturing methods.The emerging manufacturing technology of three-dimensional(3D)printing as an alternative has shown exciting applications in the medical field and is an ideal method for manufacturing such personalized medical devices with complex structures.However,the application of this new technology has also brought new risks to medical devices,making 3D-printed devices face severe challenges due to insufficient regulation and the lack of standards to provide guidance to the industry.This review aims to summarize the current regulatory landscape and existing research on the standardization of 3D-printed medical devices in China,and provide ideas to address these challenges.We focus on the aspects concerned by the regulatory authorities in 3D-printed medical devices,highlighting the quality system of such devices,and discuss the guidelines that manufacturers should follow,as well as the current limitations and the feasible path of regulation and standardization work based on this perspective.The key points of the whole process quality control,performance evaluation methods and the concept of whole life cycle management of 3D-printed medical devices are emphasized.Furthermore,the significance of regulation and standardization is pointed out.Finally,aspects worthy of attention and future perspectives in this field are discussed.

Revisiting the Role of Physical Confinement and Chemical Regulation of 3D Hosts for Dendrite-Free Li Metal Anode

Lithium metal anode has been demonstrated as the most promising anode for lithium batteries because of its high theoretical capacity,but infinite volume change and dendritic growth during Li electrodeposition have prevented its practical applications.Both physical morphology confinement and chemical adsorption/diffusion regulation are two crucial approaches to designing lithiophilic materials to alleviate dendrite of Li metal anode.However,their roles in suppressing dendrite growth for long-life Li anode are not fully understood yet.Herein,three different Ni-based nanosheet arrays(NiO-NS,Ni_(3)N-NS,and Ni_(5)P_(4)-NS)on carbon cloth as proof-of-concept lithiophilic frame-works are proposed for Li metal anodes.The two-dimensional nanoarray is more promising to facilitate uniform Li^(+)flow and electric field.Compared with the NiO-NS and the Ni_(5)P_(4)-NS,the Ni_(3)N-NS on carbon cloth after reacting with molten Li(Li-Ni/Li_(3)N-NS@CC)can afford the strongest adsorption to Li+and the most rapid Li+diffusion path.Therefore,the Li-Ni/Li_(3)N-NS@CC electrode realizes the lowest overpotential and the most excellent electrochemical performance(60 mA cm^(−2)and 60 mAh cm^(−2)for 1000 h).Furthermore,a remarkable full battery(LiFePO_(4)||Li-Ni/Li_(3)N-NS@CC)reaches 300 cycles at 2C.This research provides valuable insight into designing dendrite-free alkali metal batteries.

Aberrant methylation and downregulation of sall3 in human hepatocellular carcinoma

AIM: To investigated whether sall3 transcription was regulated by promoter CpG island hypermethylation in hepatocellular carcinoma (HCC). METHODS: The cell lines Huh7, HepG2, SK-HEP1, SM-MC7721, Bel7402, QGY7703 and a cohort of 38 HCC tissue specimens and corresponding nontumorous tissues were subjected to analysis for sall3 promoter CpG island methylation and mRNA transcription. sall3 promoter CpG island methylation levels were determined using the MassARRAY platform and mRNA transcription levels of the gene were detected by quantitative realtime polymerase chain reaction.RESULTS: The levels of sall3 mRNA were decreased by more than twofold in 33 of 38 tumor tissues compared to adjacent noncancerous tissues. Among these 33 tumor tissues with lower levels of sall3 mRNA, 24 showed higher levels of methylation. Based on these results, we hypothesized that the decrease in sall3 mRNA transcription level was likely due to promoter CpG island hypermethylation. Changes in sall3 mRNA transcription and promoter CpG island methylation were determined in the above six cell lines after treatment with 0, 0.1, 0.5 and 2.5 mmol 5-aza-2-deoxycytidine, a demethylating agent. Promoter CpG island methylation levels de- creased in a dose-dependent manner in all six cell lines, while the mRNA transcription level increased dose-dependently in Huh7, HepG2, SK-HEP1 and SMMC7721 cells and irregularly in Bel7402 and QGY7703 cells. CONCLUSION: These results indicated that promoter CpG island hypermethylation contributes to the downregulation of sall3 mRNA transcription in HCC.

Study of band 3 protein and intraerythrocytic acid-base regulation under chronic isobaric hypoxia and hypoxia-hypercapnia in rats

The changes of the structure and content of the erythrocyte membrane band 3 protein and its function of anion transport and blood gases inside and out of the erythrocytes were observed under isobaric hypoxia and hypoxia-hypercapnia in rats. It was found t

Research on the attitude regulation of 3-DOF hover system

Quadrotor helicopter is emerging as a popular platform for unmanned aerial vehicle re- search, due to its simplicity of structure and maintenance as well as the capability of hovering and vertical take-off and landing. The attitude controller is an important feature of quadrotor helicopter since it allows the vehicle to keep balance and perform the desired maneuver. In this paper, nonlin- ear control strategies including active disturbance rejection control （ADRC）, sliding mode control （SMC） and backstepping method are studied and implemented to stabilize the attitude of a 3-DOF hover system. ADRC is an error-driven control law, with extended state observer （ESO） estimating the unmodeled inner dynamics and external disturbance to dynamically compensate their impacts. Meanwhile; both backstepping technique and SMC are developed based on the mathematical model, whose stability is ensured by Lyapunov global stability theorem. Furthermore, the performance of each control algorithm is evaluated by experiments. The results validate effectiveness of the strate- gies for attitude regulation. Finally, the respective characteristics of the three controllers are high- lighted by-comparison, and conclusions are drawn on the basis of the theoretical and experimental a- nalysis.

Translational regulation of DNA repair systems by eIF3a in cancer chemotherapeutic response

OBJECTIVE To investigate the role of e IF3a in the regulation of DNA repair pathways in cancer chemotherapeutic response.METHODS Immunohistochemistry was used to determine the expression of e IF3a in lung and breast cancer tissues followed by association analysis of e IF3a expression with patient′s response to chemotherapy.Ectopic overexpression and RNA interference knockdown of e IF3a were carried out in NIH3T3and H1299 cell lines,respectively,to determine the effect of altered e IF3a expression on cellular response to chemotherapeutic drugs by using MTT assay.The DNA repair capacity of these cells was evaluated by using host-cell reactivation,NHEJ and HR assay.Real-time reverse transcriptase PCR and Western Blot analyses were carried out to determine the effect of e IF3a on the DNA repair genes by using cells with altered e IF3a expression.RESULTS e IF3a expression associates with response of lung and breast cancer patients to platinum and anthracycline.e IF3a knockdown or overexpression,respectively,increased and decreased the cellular resistance to cisplatin and anthracycline anticancer drugs,DNA repair activity,and expression of NER and NHEJ DNA repair proteins.CONCLUSION e IF3a plays an important role in regulating the expression of NER and NHEJ DNA repair proteins which,in turn,contributes to cellular response to DNA-damaging anticancer drugs and patients′response to platinum and anthracycline chemotherapy.

Magnetoelectric coupling effect of polarization regulation in BiFeO_(3)/LaTiO_(3)heterostructures

An effective regulation of the magnetism and interface of ferromagnetic materials is not only of great scientific significance,but also has an urgent need in modern industry.In this work,by using the first-principles calculations,we demonstrate an effective approach to achieve non-volatile electrical control of ferromagnets,which proves this idea in multiferroic heterostructures of ferromagnetic La TiO_(3)and ferroelectric Bi FeO_(3).The results show that the magnetic properties and two-dimensional electron gas concentrations of La TiO_(3)films can be controlled by changing the polarization directions of Bi FeO_(3).The destroyed symmetry being introduced by ferroelectric polarization of the system leads to the transfer and reconstruction of the Ti-3 d electrons,which is the fundamental reason for the changing of magnetic properties.This multiferroic heterostructures will pave the way for non-volatile electrical control of ferromagnets and have potential applications.

Boosted urea electro-oxidation over Ni_(3)N-based nanocomposite via systematic regulation tactic

Exploiting high-efficiency Ni-based materials for electrocatalytic urea oxidation reaction(UOR) is critical for urea-related technologies.The catalytic site density,intrinsic activity,charge transfer,and mass diffusion determine overall electrocatalytic efficiency.Simultaneous modulation over the above four factors promises advanced electrocatalysis,yet challenging.Herein we propose a systematic regulation tactic over composition and geometric structure,constructing a nanocomposite comprising Mn doped Ni_(3)N nanoparticles anchored on reduced graphene oxide(rGO/Mn-Ni_(3)N),achieving elegant integration of four design principles into one,thereby eminently boosting UOR.Particularly,Mn doping in Ni_(3)N can modulate electronic state to induce intrinsic activity regulation.Combining metallic Mn-Ni_(3)N with rGO to engineer hierarchical architecture not only promotes charge transfer,but also enriches active site population.Intriguingly,improved hydrophilicity could impart better electrolyte penetration and gas escape.Consequently,such system-optimized rGO/Mn-Ni_(3)N demonstrates state-of-the-art-level UOR electrocatalysis.This work offers a novel paradigm to create advanced catalysts via systematic and integrated modulation.

Facet Regulation of Fe_(2)O_(3) via Nanoarray Architecture to Enable High Faradic Efficiency for Electrocatalytic Nitrogen Fixation

We propose a facile facet regulation enabled by nanoarray architecture to achieve a high faradic efficiency of Fe_(2)O_(3) catalyst for NRR. The a-Fe_(2)O_(3) nanorod arrays (NAs) were directly grown on carbon cloth (CC) with specific (104) facet exposure. The highly exposed (104) facets provide abundant unsaturated Fe atoms with dangling bonds as nitrogen reduction reaction catalytically active sites. In addition, the NAs architecture enables the enhanced electrochemical surface area (ECSA) to fully manifest the active sites and maintain the mass diffusion. Thus, the selectively exposed (104) facets coupled with the high ECSA of NAs architecture achieve a high FE of 14.89% and a high yield rate of 17.28 μg h^(-1) cm^(-2). This work presents an effective strategy to develop highly efficient catalytic electrodes for electrochemical NRR via facet regulation and nanoarray architecture.

Research Progress in Function and Regulation of E3 Ubiquitin Ligase SMURF1

Smad ubiquitylation regulatory factor 1(Smurf1)is an important homologous member of E6-AP C-terminus type E3 ubiquitin ligase.Initially,Smurf1 was reportedly involved in the negative regulation of the bone morphogenesis protein(BMP)pathway.After further research,several studies have confirmed that Smurf1 is widely involved in various biological processes,such as bone homeostasis regulation,cell migration,apoptosis,and planar cell polarity.At the same time,recent studies have provided a deeper understanding of the regulatory mechanisms of Smurf1’s expression,activity,and substrate selectivity.In our review,a brief summary of recent important biological functions and regulatory mechanisms of E3 ubiquitin ligase Smurf1 is proposed.

姜黄素通过调控SIRT3/SOD2信号通路对阿霉素引起心肌细胞毒性的减轻作用

目的:探讨姜黄素改善阿霉素(DOX)诱导的心肌H9c2细胞毒性的作用,并阐明其作用机制。方法:采用DOX处理H9c2细胞建立心肌细胞毒性模型。将H9c2细胞分为正常组、DOX组、DOX+姜黄素组和DOX+姜黄素+沉默信息调节蛋白3(SIRT3)抑制剂3-TYP+DOX组(DOX+姜黄素+3-TYP组)。24 h后观察各组细胞形态表现,CCK-8法检测各组细胞活性,TUNEL染色检测各组细胞凋亡率,酶联免疫吸附试验(ELISA)法检测各组细胞中过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性及丙二醛(MDA)水平,2,7-二氯荧光素二乙酸酯(DCFH-DA)染色检测各组细胞中活性氧(ROS)水平,Western blotting法检测各组细胞中B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶2(NOX2)、NADPH氧化酶4(NOX4)、SIRT3、乙酰化超氧化物歧化酶2(Ac-SOD2)和超氧化物歧化酶2(SOD2)蛋白表达水平。结果:与对照组比较,DOX组H9c2细胞肿胀,细胞活性降低(P<0.05),细胞中CAT和SOD活性降低(P<0.05),MDA和ROS水平升高(P<0.05),NOX2和NOX4蛋白表达水平升高(P<0.05),Bcl-2/Bax比值和SIRT3蛋白表达水平降低(P<0.05),SOD和Ac-SOD2蛋白表达水平升高(P<0.05);与DOX组比较,DOX+姜黄素组H9c2细胞形态改善,细胞活性升高(P<0.05),细胞中CAT和SOD活性升高(P<0.05),MDA和ROS水平降低(P<0.05),NOX2和NOX4蛋白表达水平降低(P<0.05),Bcl-2/Bax比值和SIRT3蛋白表达水平升高(P<0.05),SOD2和AcSOD2蛋白表达水平降低(P<0.05);与DOX+姜黄素组比较,DOX+姜黄素+3-TYP组细胞肿胀,细胞密度和细胞活性明显降低(P<0.05),细胞凋亡率明显升高(P<0.05),细胞中CAT和SOD活性降低(P<0.05),MDA水平和ROS水平明显升高(P<0.05),NOX2和NOX 4蛋白表达水平升高(P<0.05),Bcl-2/Bax比值和SIRT3蛋白表达水平降低(P<0.05),SOD2和Ac-SOD2蛋白表达水平升高(P<0.05)。结论:姜黄素通过激活SIRT3/SOD2信号抑制氧化应激和细胞凋亡,改善细胞活性,减轻DOX引起的心肌H9c2细胞毒性。

NLRP3炎性小体在治疗性浅低温后处理大鼠心肌缺血-再灌注中的作用

目的 分析NOD样受体热蛋白结构域相关蛋白3(NLRP3)炎性小体在治疗性浅低温(34℃)后处理的大鼠离体心肌缺血-再灌注模型中的作用并探讨其机制。方法 选择清洁级成年雄性SD大鼠60只,7~10周龄,体重250~300 g。采用随机数字表法将大鼠分为五组:空白对照组(S组)、心肌缺血-再灌注组(IR组)、34℃浅低温后处理心肌缺血-再灌注组(MH组)、34℃浅低温后处理心肌缺血-再灌注+3-TYP组(HT组)和34℃浅低温后处理心肌缺血-再灌注+3-TYP+MCC950组(HTM组),每组12只。S组在37℃灌流液平衡灌流大鼠心脏180 min;IR组在37℃灌流液平衡灌流大鼠心脏30 min后,缺血30 min, 37℃灌注液再灌注120 min;MH组在37℃灌流液平衡灌流大鼠心脏30 min后,缺血30 min, 34℃灌注液再灌注120 min;HT组在37℃灌流液平衡灌流大鼠心脏30 min后,缺血30 min,在灌注液中加入沉默信息调节因子2同源蛋白3(sirt3)抑制剂3-TYP后行34℃灌注液再灌注120 min;HTM组在37℃灌流液平衡灌流大鼠心脏30 min后,缺血30 min,在灌注液中加入sirt3抑制剂3-TYP和NLRP3抑制剂MCC950后行34℃灌注液再灌注120 min。再灌注120 min后取离体心脏,采用ELISA法测定灌注后心脏漏液中IL-1β、IL-6浓度,Western blot法检测心肌组织中NLRP3和sirt3蛋白相对含量,1%氯化三苯基四氮唑染色计算心肌梗死面积,HE染色观察心肌病理变化。结果 与S组比较,IR组、MH组、HT组和HTM组再灌注30、60、90、120 min时HR明显减慢,LVSP、dp/dt_(max)明显降低,LVEDP明显升高;心脏漏液中IL-6和IL-1β浓度、心肌梗死面积百分比明显升高(P<0.05);IR组、HT组和HTM组心肌组织中sirt3蛋白含量明显降低,NLRP3蛋白含量明显升高(P<0.05);MH组心肌组织中sirt3和NLRP3蛋白含量明显升高(P<0.05)。与IR组比较,MH组和HTM组再灌注30、60、90、120 min时HR明显增快,LVSP、±dp/dt_(max)明显升高,LVEDP明显降低;心脏漏液中IL-6和IL-1β浓度、心肌梗死面积百分比明显降低(P<0.05);MH组心肌组织中sirt3蛋白含量明显升高,NLRP3蛋白含量明显降低(P<0.05);HTM组心肌组织中NLRP3蛋白含量明显降低(P<0.05)。与MH组比较,HT组再灌注30、60、90、120 min时HR明显减慢,LVSP、±dp/dt_(max)明显降低,LVEDP明显升高;心脏漏液中IL-6和IL-1β浓度、心肌梗死面积百分比、心肌组织中NLRP3蛋白含量明显升高(P<0.05);HT组和HTM组心肌组织中sirt3蛋白含量明显降低(P<0.05)。与HT组比较,HTM组再灌注30、60、90、120 min时HR明显增快,LVSP、±dp/dt_(max)明显升高,LVEDP明显降低;心脏漏液中IL-6和IL-1β浓度、心肌梗死面积百分比、心肌组织中NLRP3蛋白含量明显降低(P<0.05)。结论 治疗性浅低温(34℃)可通过改善离体心脏血流动力学参数、降低IL-6、IL-1β浓度、心肌组织中NLRP3蛋白含量、心肌梗死面积百分比、改善心肌病理学改变,减轻大鼠心肌缺血-再灌注损伤,其机制可能与线粒体介导sirt3通路抑制炎性小体NLRP3的高表达有关。

冠心病患者血清环磷酸腺苷反应元件结合蛋白调节转录辅激活因子3及氧化应激指标与颈动脉粥样硬化的相关性

目的探讨冠心病患者血清环磷酸腺苷反应元件结合蛋白调节转录辅激活因子3(CRTC3)及氧化应激指标与颈动脉粥样硬化的相关性。方法选取2021年6月—2023年6月本院收治的154例冠心病患者为研究组,根据颈动脉粥样硬化程度分为轻度硬化组、中度硬化组和重度硬化组;另选取154例同期健康体检者为对照组。采用Pearson法分析血清CRTC3及氧化应激指标与颈动脉粥样硬化指标的相关性。结果研究组血清CRTC3、丙二醛(MDA)、颈动脉斑块面积和中层内膜厚度(IMT)高于或大于对照组,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平低于对照组,差异有统计学意义(P<0.05)。轻度硬化组、中度硬化组和重度硬化组血清CRTC3、MDA、颈动脉斑块面积和IMT依次升高或增大,SOD、GSH-Px水平依次降低,差异有统计学意义(P<0.05)。Pearson相关性分析显示,血清CRTC3、MDA水平与颈动脉斑块面积、IMT呈正相关(P<0.05),SOD、GSH-Px与颈动脉斑块面积、IMT呈负相关(P<0.05)。受试者工作特征(ROC)曲线显示,CRTC3、SOD、MDA和GSH-Px联合诊断重度颈动脉粥样硬化的曲线下面积(AUC)为0.990(95%CI:0.982~0.998),灵敏度为96.27%,特异度为76.28%。4项指标联合诊断的价值高于各指标单独诊断,差异有统计学意义(Z_(联合-CRTC3)=2.723,Z_(联合-SOD)=2.698,Z_(联合-MDA)=2.673,Z_(联合-GSH-Px)=2.803,P均<0.05)。结论冠心病患者血清CRTC3、MDA水平显著升高,SOD、GSH-Px水平显著降低;血清CRTC3、氧化应激水平均与颈动脉粥样硬化密切相关。

皮肤鳞状细胞癌组织Sirt3、DAB2IP表达与病理特征相关性及对术后复发的预测意义

目的分析皮肤鳞状细胞癌(cSCC)组织沉默信息调节因子3(Sirt3)、DOC2/DAB2相互作用蛋白(DAB2IP)表达与病理特征的关系,并分析二者对术后复发的预测价值。方法选取2018年7月—2020年6月本院cSCC患者193例,均行扩大切除病灶手术。观察cSCC患者肿瘤组织与癌旁组织Sirt3、DAB2IP表达情况,统计术后复发情况并比较术后复发与未复发cSCC患者的相关资料,采用Logistic回归分析cSCC患者术后复发的影响因素,采用受试者工作特征(ROC)曲线分析Sirt3、DAB2IP预测cSCC患者术后复发的价值,绘制Sirt3、DAB2IP预测cSCC患者术后复发的决策曲线并进行分析,不同Sirt3、DAB2IP表达的cSCC患者术后复发情况。结果cSCC患者肿瘤组织Sirt3、DAB2IP高表达例数多于低表达,差异有统计学意义(P<0.05);193例患者术后复发42例,未复发151例,术后复发的cSCC患者病灶直径、分化程度、术后切缘阳性、Sirt3及DAB2IP表达情况与未复发患者比较,差异有统计学意义(P<0.05);分化程度为低分化、术后切缘阳性Sirt3及DAB2IP高表达是cSCC患者术后复发的独立危险因素(P<0.05);Sirt3、DAB2IP联合检测预测cSCC患者术后复发的曲线下面积(AUC)值为0.669,高于二者单独检测;决策曲线分析显示,当风险阈值为0.20~0.45时,Sirt3、DAB2IP联合检测的净受益率大于单独检测;Sirt3+/DAB2IP+的cSCC患者术后复发率明显高于Sirt3+/DAB2IP-、Sirt3-/DAB2IP+及Sirt3-/DAB2IP-的患者(P<0.05)。结论cSCC组织中Sirt3、DAB2IP均呈高表达,二者同时高表达表明患者术后复发风险较高,可通过检测二者表达情况为临床早期筛查提供依据。

通络熄风汤通过调控Mst1/Sirt3信号通路对自发性高血压大鼠心肌损伤程度的影响

目的:探讨通络熄风汤改善高血压大鼠心肌损伤的作用机制。方法:6只Sprague-Dawley大鼠作为空白组,12只自发性高血压大鼠随机分为模型组和通络熄风汤组。空白组和模型组大鼠给予去离子水灌胃,通络熄风汤组给予中药配方颗粒通络熄风汤悬浮液灌胃治疗,连续干预8周。采用蛋白免疫印迹法(Western Blot)检测心肌细胞中自噬受体蛋白1(SQSTM1/P62)、磷酸化哺乳动物无菌20样激酶1(p-Mst1)、沉默信息调节因子3(Sirt3)蛋白的含量,运用苏木精-伊红(HE)染色观察大鼠心肌组织结构变化,以评价通络熄风汤对高血压心肌损伤改善的效果及机制。结果:与模型组比较,通络熄风汤组能够显著改善高血压大鼠的收缩压及舒张压(P<0.01)。通络熄风汤治疗后大鼠心肌细胞与模型组比较,排列情况与坏死状态均有明显改善。通络熄风汤组大鼠心肌组织中的p-Mst1、P62蛋白含量较模型组降低(P<0.01),Sirt3蛋白含量升高(P<0.01)。结论:通络熄风汤可以介导Mst1/Sirt3信号通路对心肌组织细胞自噬水平进行调整,并且保护心脏功能。

胃癌患者癌组织LDOC1、GNL3L表达及其与病理特征、预后的关系

目的探讨胃癌患者癌组织中亮氨酸拉链下调因子1(LDOC1)、鸟嘌呤核苷酸结合蛋白样3-样蛋白(GNL3L)表达水平及与患者病理特征、预后的关系。方法收集2019年12月至2022年12月新乡市中心医院收治的65例胃癌患者癌组织标本(胃癌组)及对应癌旁组织标本(癌旁组)。采用免疫组化法检测组织中LDOC1、GNL3L表达水平。采用χ^(2)检验分析LDOC1、GNL3L表达与胃癌患者临床病理特征的关系,多因素Cox回归分析探讨影响胃癌患者预后的相关因素。结果胃癌组LDOC1阳性表达率(30.76%)低于癌旁组(61.52%),GNL3L阳性表达率(72.31%)高于癌旁组(43.07%)(χ^(2)=12.381、11.377,P<0.05)。低分化、肿瘤直径>3 cm、TNM分期Ⅲ/Ⅳ期、淋巴结转移的胃癌患者LDOC1阴性率、GNL3L阳性率高于中/高分化、肿瘤直径≤3 cm、TNM分期Ⅰ/Ⅱ期、无淋巴结转移患者(P<0.05)。LDOC1阳性表达患者3 a生存率(85.00%)高于LDOC1阴性表达患者(37.77%),GNL3L阳性表达患者3 a生存率(36.17%)低于GNL3L阴性表达患者(94.44%)(P<0.05)。低分化、TNM分期Ⅲ/Ⅳ期、淋巴结转移胃癌患者的3 a生存率低于中/高分化、TNM分期Ⅰ/Ⅱ期、无淋巴结转移患者(P<0.05)。多因素Cox回归分析显示,TNM分期Ⅲ~Ⅳ期(HR=2.113,95%CI:1.425~3.133)、淋巴结转移(HR=2.625,95%CI:1.564~4.404)、LDOC1阴性表达(HR=5.607,95%CI:3.408~9.224)、GNL3L阳性表达(HR=2.930,95%CI:1.716~5.003)是胃癌患者预后的危险因素(P<0.05)。结论LDOC1在胃癌患者中呈低表达,GNL3L在胃癌患者中呈高表达,二者均与患者临床病理特征、预后有关。