Improving plant resistance to Verticillium wilt(VW),which causes massive losses in Gossypium hirsutum,is a global challenge.Crop plants need to efficiently allocate their limited energy resources to maintain a balance...Improving plant resistance to Verticillium wilt(VW),which causes massive losses in Gossypium hirsutum,is a global challenge.Crop plants need to efficiently allocate their limited energy resources to maintain a balance between growth and defense.However,few transcriptional regulators specifically respond to Verticillium dahliae and the underlying mechanism has not been identified in cotton.In this study,we found that the that expression of most R2R3-MYB members in cotton is significantly changed by V.dahliae infection relative to the other MYB types.One novel R2R3-MYB transcription factor(TF)that specifically responds to V.dahliae,GhMYB3D5,was identified.GhMYB3D5 was not expressed in 15 cotton tissues under normal conditions,but it was dramatically induced by V.dahliae stress.We functionally characterized its positive role and underlying mechanism in VW resistance.Upon V.dahliae infection,the up-regulated GhMYB3D5 bound to the GhADH1 promoter and activated GhADH1expression.In addition,GhMYB3D5 physically interacted with GhADH1 and further enhanced the transcriptional activation of GhADH1.Consequently,the transcriptional regulatory module GhMYB3D5-GhADH1 then promoted lignin accumulation by improving the transcriptional levels of genes related to lignin biosynthesis(GhPAL,GhC4H,Gh4CL,and GhPOD/GhLAC)in cotton,thereby enhancing cotton VW resistance.Our results demonstrated that the GhMYB3D5 promotes defense-induced lignin accumulation,which can be regarded as an effective way to orchestrate plant immunity and growth.展开更多
目的搭建基于4K/3D手术系统的远程手术会诊平台,评价5G通信技术支撑下4K分辨率3D样式画面在显微外科远程手术会诊中的应用效果。方法设计基于“端云端”的系统软硬件架构,依托国产4K/3D显微手术成像系统,实现术野的双视点光场采集、智...目的搭建基于4K/3D手术系统的远程手术会诊平台,评价5G通信技术支撑下4K分辨率3D样式画面在显微外科远程手术会诊中的应用效果。方法设计基于“端云端”的系统软硬件架构,依托国产4K/3D显微手术成像系统,实现术野的双视点光场采集、智能图像处理、硬件3D交织、低延时网络传输和超高清立体显示。将该系统应用于侧颅底疑难病例的远程手术会诊,从4K/3D视频传输延时、峰值信噪比(Peak Signal to Noise Ratio,PSNR)、结构相似性(Structural Similarity Index Measure,SSIM)、画质及舒适度等方面评价该平台的效能。结果在非专用5G组网方案下,手术会诊平台的4K/3D视频传输延时1.18 s;评价视频传输质量的客观指标PSNR和SSIM显示4K/3D视频传输前后的视觉差异和结构差异小。27名医生针对8段手术视频片段的问卷调查结果显示,视频传输后画质无改变或改变轻微不影响观看的样本占比为89.35%,96.30%的医生认为远程传输后的4K/3D视频足以支撑显微外科远程手术指导。结论4K/3D显微手术成像系统产出的超高清立体视频经5G传输后足以支撑远程手术指导,这为远程医疗及教学、灾难及战场手术救治提供了新方案。展开更多
基金supported by the National Key Research and Development Program of China(2022YFF1001403)the Natural Science Foundation of Hebei Province,China(C2022204205)+1 种基金the National Natural Science Foundation of China(32372194)the National Top Talent Project and Hebei Top Talent,China。
文摘Improving plant resistance to Verticillium wilt(VW),which causes massive losses in Gossypium hirsutum,is a global challenge.Crop plants need to efficiently allocate their limited energy resources to maintain a balance between growth and defense.However,few transcriptional regulators specifically respond to Verticillium dahliae and the underlying mechanism has not been identified in cotton.In this study,we found that the that expression of most R2R3-MYB members in cotton is significantly changed by V.dahliae infection relative to the other MYB types.One novel R2R3-MYB transcription factor(TF)that specifically responds to V.dahliae,GhMYB3D5,was identified.GhMYB3D5 was not expressed in 15 cotton tissues under normal conditions,but it was dramatically induced by V.dahliae stress.We functionally characterized its positive role and underlying mechanism in VW resistance.Upon V.dahliae infection,the up-regulated GhMYB3D5 bound to the GhADH1 promoter and activated GhADH1expression.In addition,GhMYB3D5 physically interacted with GhADH1 and further enhanced the transcriptional activation of GhADH1.Consequently,the transcriptional regulatory module GhMYB3D5-GhADH1 then promoted lignin accumulation by improving the transcriptional levels of genes related to lignin biosynthesis(GhPAL,GhC4H,Gh4CL,and GhPOD/GhLAC)in cotton,thereby enhancing cotton VW resistance.Our results demonstrated that the GhMYB3D5 promotes defense-induced lignin accumulation,which can be regarded as an effective way to orchestrate plant immunity and growth.
文摘目的搭建基于4K/3D手术系统的远程手术会诊平台,评价5G通信技术支撑下4K分辨率3D样式画面在显微外科远程手术会诊中的应用效果。方法设计基于“端云端”的系统软硬件架构,依托国产4K/3D显微手术成像系统,实现术野的双视点光场采集、智能图像处理、硬件3D交织、低延时网络传输和超高清立体显示。将该系统应用于侧颅底疑难病例的远程手术会诊,从4K/3D视频传输延时、峰值信噪比(Peak Signal to Noise Ratio,PSNR)、结构相似性(Structural Similarity Index Measure,SSIM)、画质及舒适度等方面评价该平台的效能。结果在非专用5G组网方案下,手术会诊平台的4K/3D视频传输延时1.18 s;评价视频传输质量的客观指标PSNR和SSIM显示4K/3D视频传输前后的视觉差异和结构差异小。27名医生针对8段手术视频片段的问卷调查结果显示,视频传输后画质无改变或改变轻微不影响观看的样本占比为89.35%,96.30%的医生认为远程传输后的4K/3D视频足以支撑显微外科远程手术指导。结论4K/3D显微手术成像系统产出的超高清立体视频经5G传输后足以支撑远程手术指导,这为远程医疗及教学、灾难及战场手术救治提供了新方案。
