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miR-24-3p promotes proliferation and inhibits apoptosis of porcine granulosa cells by targeting P27
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作者 Shengjie Shi Lutong Zhang +7 位作者 Liguang Wang Huan Yuan Haowei Sun Mielie Madaniyati Chuanjiang Cai Weijun Pang Lei Gao Guiyan Chu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第4期1315-1328,共14页
Ovarian follicle development is associated with the physiological functions of granulosa cells(GCs),including proliferation and apoptosis.The level of miR-24-3p in ovarian tissue of high-yielding Yorkshire×Landra... Ovarian follicle development is associated with the physiological functions of granulosa cells(GCs),including proliferation and apoptosis.The level of miR-24-3p in ovarian tissue of high-yielding Yorkshire×Landrace sows was significantly higher than that of low-yielding sows.However,the functions of miR-24-3p on GCs are unclear.In this study,using flow cytometry,5-ethynyl-2′-de-oxyuridine(EdU)staining,and cell count,we showed that miR-24-3p promoted the proliferation of GCs increasing the proportion of cells in the S phase and upregulating the expression of cell cycle genes,moreover,miR-24-3p inhibited GC apoptosis.Mechanistically,on-line prediction,bioinformatics analysis,a luciferase reporter assay,RT-qPCR,and Western blot results showed that the target gene of miR-24-3p in proliferation and apoptosis is cyclin-dependent kinase inhibitor 1B(P27/CDKN1B).Furthermore,the effect of miR-24-3p on GC proliferation and apoptosis was attenuated by P27 overexpression.These findings suggest that miR-24-3p regulates the physiological functions of GCs. 展开更多
关键词 miR-24-3p granulosa cells PROliFERAtION APOPtOSIS
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Exosomes derived from microRNA-540-3p overexpressing mesenchymal stem cells promote immune tolerance via the CD74/nuclear factor-kappaB pathway in cardiac allograft
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作者 Ji-Gang He Xin-Xin Wu +3 位作者 Si Li Dan Yan Gao-Peng Xiao Fu-Gang Mao 《World Journal of Stem Cells》 SCIE 2024年第12期1022-1046,共25页
BACKGROUND Heart transplantation is a crucial intervention for severe heart failure,yet the challenge of organ rejection is significant.Bone marrow mesenchymal stem cells(BMSCs)and their exosomes have demonstrated pot... BACKGROUND Heart transplantation is a crucial intervention for severe heart failure,yet the challenge of organ rejection is significant.Bone marrow mesenchymal stem cells(BMSCs)and their exosomes have demonstrated potential in modulating T cells,dendtitic cells(DCs),and cytokines to achieve immunomodulatory effects.DCs,as key antigen-presenting cells,play a critical role in shaping immune responses by influencing T-cell activation and cytokine production.Through this modulation,BMSCs and their exosomes enhance graft tolerance and prolonging survival.AIM To explore the immunomodulatory effects of exosomes derived from BMSCs overexpressing microRNA-540-3p(miR-540-3p)on cardiac allograft tolerance,focusing on how these exosomes modulating DCs and T cells activity through the CD74/nuclear factor-kappaB(NF-κB)pathway.METHODS Rat models were used to assess the impact of miR-540-3p-enhanced exosomes on immune tolerance in cardiac allografts.MiR-540-3p expression was manipulated in BMSCs,and derived exosomes were collected and administered to the rat models post-heart transplantation.The study monitored expression levels of major histocompatibility complex II,CD80,CD86,and CD274 in DCs,and quantified CD4^(+)and CD8^(+)T cells,T regulatory cells,and cytokine profiles.RESULTS Exosomes from miR-540-3p-overexpressing BMSCs lead to reduced expression of immune activation markers CD74 and NF-κB p65 in DCs and T cells.Rats treated with these exosomes showed decreased inflammation and improved cardiac function,indicated by lower levels of pro-inflammatory cytokines(interleukin-1β,interferon-γ)and higher levels of anti-inflammatory cytokines(interleukin-10,transforming growth factorβ1).Additionally,miR-540-3p skewed the profiles of DCs and T cells towards immune tolerance,increasing the ratio of T regulatory cells and shifting cytokine secretion to favor graft acceptance.CONCLUSION Exosomes derived from BMSCs overexpressing miR-540-3p significantly enhance immune tolerance and prolong cardiac allograft survival by modulating the CD74/NF-κB pathway,which regulates activities of DCs and T cells.These findings highlight a promising therapeutic strategy to improve heart transplantation outcomes and potentially reduce the need for prolonged immunosuppression. 展开更多
关键词 Bone marrow mesenchymal stem cells EXOSOMES MicroRNA-540-3p Cardiac allograft Immune tolerance
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RSSI-Based 3D Wireless Sensor Node Localization Using Hybrid T Cell Immune and Lotus Optimization
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作者 Weiwei Hu Kiran Sree Pokkuluri +3 位作者 Rajesh Arunachalam Bander A.Jabr Yasser A.Ali Preethi Palanisamy 《Computers, Materials & Continua》 SCIE EI 2024年第12期4833-4851,共19页
Wireless Sensor Network(WSNs)consists of a group of nodes that analyze the information from surrounding regions.The sensor nodes are responsible for accumulating and exchanging information.Generally,node local-ization... Wireless Sensor Network(WSNs)consists of a group of nodes that analyze the information from surrounding regions.The sensor nodes are responsible for accumulating and exchanging information.Generally,node local-ization is the process of identifying the target node’s location.In this research work,a Received Signal Strength Indicator(RSSI)-based optimal node localization approach is proposed to solve the complexities in the conventional node localization models.Initially,the RSSI value is identified using the Deep Neural Network(DNN).The RSSI is conceded as the range-based method and it does not require special hardware for the node localization process,also it consumes a very minimal amount of cost for localizing the nodes in 3D WSN.The position of the anchor nodes is fixed for detecting the location of the target.Further,the optimal position of the target node is identified using Hybrid T cell Immune with Lotus Effect Optimization algorithm(HTCI-LEO).During the node localization process,the average localization error is minimized,which is the objective of the optimal node localization.In the regular and irregular surfaces,this hybrid algorithm effectively performs the localization process.The suggested hybrid algorithm converges very fast in the three-dimensional(3D)environment.The accuracy of the proposed node localization process is 94.25%. 展开更多
关键词 Sensor node localization received signal strength indicator 3D wireless sensor network deep neural network average localization error and hybrid t cell immune with lotus effect optimization algorithm
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丹益活血汤加减对产科抗磷脂综合征相关复发性流产患者血清Tim-3、Gal-9、RAGE水平的影响
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作者 钱雅婷 周雪 朱轶庆 《陕西中医》 CAS 2025年第1期38-41,共4页
目的:探讨丹益活血汤对产科抗磷脂综合征相关复发性流产(OAPS-RSA)患者血清黏蛋白域分子3(Tim-3)、半乳糖凝集素9(Gal-9)、晚期糖基化终末产物受体(RAGE)水平的影响。方法:选取128例OAPS-RSA患者,随机分为两组,对照组给予小剂量阿司匹林... 目的:探讨丹益活血汤对产科抗磷脂综合征相关复发性流产(OAPS-RSA)患者血清黏蛋白域分子3(Tim-3)、半乳糖凝集素9(Gal-9)、晚期糖基化终末产物受体(RAGE)水平的影响。方法:选取128例OAPS-RSA患者,随机分为两组,对照组给予小剂量阿司匹林(LDA)联合低分子量肝素(LMWH)治疗,试验组加服丹益活血汤治疗,每组64例。比较两组疗效、治疗前后的子宫内膜容受性(ER)、血栓前状态(PTS)、血清Tim-3、Gal-9、RAGE水平及妊娠结局。结果:试验组总有效率更高(90.63%与76.56%)(P<0.05)。治疗后,与对照组比较,试验组EMT、Tim-3、Gal-9、RAGE均显著升高(P<0.05),PI、RI、FDP、D-D均显著降低(P<0.05)。试验组活产率高于对照组(P<0.05)。结论:丹益活血汤能够提升OAPS-RSA患者疗效,改善ER和PTS,升高血清Tim-3、Gal-9、RAGE水平,改善妊娠结局。 展开更多
关键词 子宫内膜容受性 复发性流产 丹益活血汤 产科抗磷脂综合征 黏蛋白域分子3 半乳糖凝集素9
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Ni doped La_(0.6)Sr_(0.4)FeO_(3-δ) symmetrical electrode for solid oxide fuel cells 被引量:1
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作者 马朝晖 孙春文 +3 位作者 马超 吴昊 占忠亮 陈立泉 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 北大核心 2016年第8期1347-1353,共7页
The conventional Ni cermet anode suffers from severe carbon deposition and sulfur poisoning when fossil fuels are used. Alternative anode materials are desired for high performance hydrocarbon fuel solid oxide fuel ce... The conventional Ni cermet anode suffers from severe carbon deposition and sulfur poisoning when fossil fuels are used. Alternative anode materials are desired for high performance hydrocarbon fuel solid oxide fuel cells (SOFCs). We report the rational design of a very active Ni doped La0.6Sr0.4FeO3‐δ(LSFN) electrode for hydrocarbon fuel SOFCs. Homogeneously dispersed Ni‐Fe alloy nanoparticles were in situ extruded onto the surface of the LSFN particles during the operation of the cell. Sym‐metric SOFC single cells were prepared by impregnating a LSFN precursor solution onto a YSZ (yt‐tria stabilized zirconia) monolithic cell with a subsequent heat treatment. The open circuit voltage of the LSFN symmetric cell reached 1.18 and 1.0 V in humidified C3H8 and CH4 at 750??, respective‐ly. The peak power densities of the cells were 400 and 230 mW/cm2 in humidified C3H8 and CH4, respectively. The electrode showed good stability in long term testing, which revealed LSFN has good catalytic activity for hydrocarbon fuel oxidation. 展开更多
关键词 Solid oxide fuel cells Ni dopedLa0.6Sr0.4FeO3-δ Symmetrical electrode Hydrocarbon fuels
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The intracellular mechanism of alpha-fetoprotein promoting the proliferation of NIH 3T3 cells 被引量:27
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作者 MENG SEN LI, PING FENG LI, FBI YI YANG, SHI PENG HE, Guo GUANG DU, GANG LI1 Department of Biochemistry and Molecular Biology, 2 Department of Biophysics, Health Science Center, Peking University, Beijing 100083, China 《Cell Research》 SCIE CAS CSCD 2002年第2期151-156,共6页
AIM: The existence and properties of alpha-fetoprotein (AFP) receptor on the surface of NIH 3T3 cells and the effects of AFP on cellular signal transduction pathway were investigated. METHODS: The effect of AFP on the... AIM: The existence and properties of alpha-fetoprotein (AFP) receptor on the surface of NIH 3T3 cells and the effects of AFP on cellular signal transduction pathway were investigated. METHODS: The effect of AFP on the proliferation of NIH 3T3 cells was measured by incorporation of 3H-TdR. Receptor-binding assay of 125I-AFP was performed to detect the properties of AFP receptor in NIH 3T3 cells. The influences of AFP on the [cAMP]i and the activities of protein kinase A (PKA) were determined. Western blot was used to detect the change of K-ras P21 protein expression. RESULTS: The proliferation of NIH 3T3 cells treated with 0-80 mg/L of AFP was significantly enhanced. The Scatchard analysis indicated that there were two classes of binding sites with KD of 2.722 x 10(-9)M (Bmax=12810 sites per cell) and 8.931 x 10(-8)M (Bmax=119700 sites per cell) respectively. In the presence of AFP (20 mg/L), the content of cAMP and activities of PKA were significantly elevated . The level of K-ras P21 protein was upregulated by AFP at the concentration of 20 mg/L. The monoclonal antibody against AFP could reverse the effects of AFP on the cAMP content, PKA activity and the expression of K-ras p21 gene. CONCLUSION: The effect of AFP on the cell proliferation was achieved by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 gene. 展开更多
关键词 3t3 cells Animals cell Division Cyclic AMP Cyclic AMP-Dependent Protein Kinases Dose-Response Relationship Drug Humans Mice Receptors Peptide Research Support Non-U.S. Gov't Signal transduction time Factors ALPHA-FEtOPROtEINS
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Interaction of 14-3-3σ with KCMF1 suppresses the proliferation and colony formation of human colon cancer stem cells 被引量:5
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作者 Jian Zou Lin Mi +1 位作者 Xiao-Feng Yu Jie Dong 《World Journal of Gastroenterology》 SCIE CAS 2013年第24期3770-3780,共11页
AIM: To investigate the biological function of 14-3-3σ protein and to look for proteins that interact with 14-3-3σ protein in colon cancer stem cells. METHODS: Reverse transcription polymerase chain reaction was per... AIM: To investigate the biological function of 14-3-3σ protein and to look for proteins that interact with 14-3-3σ protein in colon cancer stem cells. METHODS: Reverse transcription polymerase chain reaction was performed to amplify the 14-3-3σ gene from the mRNA of colon cancer stem cells. The gene was then cloned into the pGEM-T vector. After being sequenced, the target gene 14-3-3σ was cut from the pGEM-T vector and cloned into the pGBKT7 yeast expression plasmid. Then, the bait plasmid pGBKT7-14-3-3σ was transformed into the yeast strain AH109. After the expression of the pGBKT7-14-3-3σ fusion protein in the AH109 yeast strain was accomplished, a yeast two-hybrid screening assay was performed by mating AH109 with Y187 that contained a HeLa cDNA library plasmid. The interaction between the 14-3-3σ protein and the proteins obtained from positive colonies was further confirmed by repeating the yeast two-hybridscreen. After extracting and sequencing the plasmids from the positive colonies, we performed a bioinformatics analysis. A coimmunoprecipitation assay was performed to confirm the interaction between 14-3-3σ and the proteins obtained from the positive colonies. Finally, we constructed 14-3-3σ and potassium channel modulatory factor 1 (KCMF1) siRNA expression plasmids and transfected them into colon cancer stem cells. RESULTS: The bait plasmid pGBKT7-14-3-3σ was constructed successfully, and the 14-3-3σ protein had no toxic or autonomous activation effect on the yeast. Nineteen true-positive colonies were selected and sequenced, and their full-length sequences were obtained. We searched for homologous DNA sequences for these sequences from GenBank. Among the positive colonies, four coding genes with known functions were obtained, including KCMF1 , quinone oxidore-ductase (NQO2 ), hydroxyisobutyrate dehydrogenase (HIBADH ) and 14-3-3σ . For the subsequent coimmu-noprecipitation assay, the plasmids PCDEF-Flag-14-3-3σ, PCDEF-Myc-KCMF1, PCDEF-Myc-NQO2 and PCDEF-Myc-HIBADH were successfully constructed, and the sequences were further confirmed by DNA sequencing. The Fugene 6 reagent was used to transfect the plasmids, and fluorescence-activated cell sorting analysis showed the transfection efficiency was 97.8% after 48 h. The HEK 293FT cells showed the stable expression of the PCDEF-Flag-14-3-3σ, PCDEF-Myc-KCMF1, PCDEF-Myc-NQO2 and PCDEF-Myc-HIBADH plasmids. After anti-Myc antibody immunoprecipitation with Myc-KCMF1, Myc-NQO2 and Myc-HIBADH from cell lysates, the presence of Flag-14-3-3σ protein in the immuno-precipitated complex was determined by western blot analysis. The knock-down expression of the 14-3-3σ and KCMF1 proteins significantly inhibited cell proliferation and colony formation of SW1116csc. CONCLUSION: Genes of the proteins that interactedwith 14-3-3σ were successfully screened from a HeLa cDNA library. KCMF1 and 14-3-3σ protein may affect the proliferation and colony formation of human colon cancer stem cells. 展开更多
关键词 14-3-3σ protein INtERACtING proteins YEASt tWO-HYBRID system COLON cancer stem cells
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Potential role of chitinase 3-like-1 in inflammation-associated carcinogenic changes of epithelial cells 被引量:9
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作者 Katrin Eurich Mayuko Segawa +1 位作者 Satoko Toei-Shimizu Emiko Mizoguchi 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第42期5249-5259,共11页
The family of mammalian chitinases includes members both with and without glycohydrolase enzymatic activity against chitin, a polymer of N-acetylglucosamine. Chitin is the structural component of fungi, crustaceans, i... The family of mammalian chitinases includes members both with and without glycohydrolase enzymatic activity against chitin, a polymer of N-acetylglucosamine. Chitin is the structural component of fungi, crustaceans, insects and parasitic nematodes, but is completely absent in mammals. Exposure to antigens containing chitin- or chitin-like structures sometimes induces strong T helper type-I responses in mammals, which may be associated with the induction of mammalian chitinases. Chitinase 3-like-1 (CHI3L1), a member of the mammalian chitinase family, is induced specifically during the course of inflammation in such disorders as inflammatory bowel disease, hepatitis and asthma. In addition, CHI3L1 is expressed and secreted by several types of solid tumors including glioblastoma, colon cancer, breast cancer and malignant melanoma. Although the exact function of CHI3L1 in inflammation and cancer is still largely unknown, CHI3L1 plays a pivotal role in exacerbating the inflammatory processes and in promoting angiogenesis and remodeling of the extracellular matrix. CHI3L1 may be highly involved in the chronic engagement of inflammation which potentiates development of epithelial tumorigenesis presumably by activating the mitogen-activated protein kinase and the protein kinase B signaling pathways. Anti-CHI3L1 antibodies or pan-chitinase inhibitors may have the potential to suppress CHI3Ll-mediated chronic inflammation and the subsequent carcinogenic change in epithelial cells. 展开更多
关键词 MAMMALS Chitinase 3-1ike 1 COLON Epithelial cells INFLAMMAtION COlitIS Colon neoplasms Inflammatory bowel disease
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Growth inhibition of hepatocellular carcinoma tumor endothelial cells by miR-204-3p and underlying mechanism 被引量:9
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作者 Zhong-hui Cui Shi-qiang Shen +1 位作者 Zu-bing Chen Chao hu 《World Journal of Gastroenterology》 SCIE CAS 2014年第18期5493-5504,共12页
AIM: To investigate the mechanism by which miR-204-3p inhibits the growth of hepatocellular carcinoma (HCC) tumor endothelial cells (TECs).
关键词 tumor vascular endothelial cells of hepatocellular carcinoma Hepatic sinusoidal endothelial cells MiRNA microarray Mir-204-3p Fibronectin 1
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MiR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine granulosa cells 被引量:6
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作者 Shengjie Shi Xiaoge Zhou +5 位作者 Jingjing Li Lutong Zhang Yamei Hu Yankun Li Gongshe Yang Guiyan Chu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2021年第1期185-197,共13页
Background: Granulosa cells(GCs) proliferation and estradiol synthesis significantly affect follicular development.The miR-214-3p expression in the ovarian tissues of high-yielding sows is higher than that in low-yiel... Background: Granulosa cells(GCs) proliferation and estradiol synthesis significantly affect follicular development.The miR-214-3p expression in the ovarian tissues of high-yielding sows is higher than that in low-yielding sows,indicating that miR-214-3p may be involved in sow fertility. However, the functions and mechanisms of miR-214-3p on GCs are unclear. This study focuses on miR-214-3p in terms of the effects on GCs proliferation and estradiol synthesis.Results: Our findings revealed that miR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine GCs. MiR-214-3p can increase the percentage of S-phase cells, the number of EdU labeled positive cells, and cell viability. However, E2 concentration was reduced after miR-214-3p agomir treatment. We also found that miR-214-3p up-regulates the expression of cell cycle genes including cell cycle protein B(Cyclin B), cell cycle protein D(Cyclin D), cell cycle protein E(Cyclin E), and cyclin-dependent kinase 4(CDK4) at the transcription and translation levels, but down-regulates the mRNA and protein levels of cytochrome P450 family 11 subfamily A member 1(CYP11A1), cytochrome P450 family 19 subfamily A member 1(CYP19A1), and steroidogenic acute regulatory protein(StAR)(i.e., the key enzymes in estradiol synthesis). On-line prediction, bioinformatics analysis, a luciferase reporter assay, RT-qPCR, and Western blot results showed that the target genes of miR-214-3p in proliferation and estradiol synthesis are Mfn2 and NR5A1, respectively.Conclusions: Our findings suggest that miR-214-3 p plays an important role in the functional regulation of porcine GCs and therefore may be a target gene for regulating follicular development. 展开更多
关键词 Estradiol synthesis Granulosa cells MiR-214-3p PROliFERAtION
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Probiotic Mixture VSL#3 Alleviates Dextran Sulfate Sodium-induced Colitis in Mice by Downregulating T Follicular Helper Cells 被引量:3
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作者 Xiao-jing LIU Ran YU Kai-fang ZOU 《Current Medical Science》 SCIE CAS 2019年第3期371-378,共8页
Clinical trials have shown beneficial effects of probiotics on inflammatory bowel diseases (IBD), although the exact mechanism remains unknown. VSL#3, a mixture of 8 probiotic bacteria, has been confirmed to have adju... Clinical trials have shown beneficial effects of probiotics on inflammatory bowel diseases (IBD), although the exact mechanism remains unknown. VSL#3, a mixture of 8 probiotic bacteria, has been confirmed to have adjunctive therapeutic effects on colitis. T follicular helper (Tfh) cells, a new separate subset of CD4+ T helper cells, have been proved to play a vital role in autoimmunity. The present study aimed to identify the beneficial effect of the probiotic mixture VSL#3 on the mouse model of colitis by regulating Tfh cells. Dextran sulfate sodium (DSS) was used to induce chronic colitis in C57BL/6 mice. VSL#3 (3x109 live bacteria) was given to C57BL/6 mice every other day for 60 days by gavage. The disease activity index (DAI), histological activity index (HAI), colon length and myeloperoxidase (MPO) activity were detected. Immunofluorescence was used to visualize the location of Tfh cells. Immunoglobulins, Tfh cells and plasma cells were quantified by enzyme-linked immunosorbent assay (ELISA), flow cytometry, real-time PCR or Western blotting. The results showed that after DSS treatment, the humoral immunity was disordered in C57BL/6 mice, with increased IgM, IgG and IgA levels in colonic mucus and increased Tfh cells in mesenteric lymph nodes (MLN). VSL#3 treatment showed anti-inflammatory effects as evidenced by reduced DAI score, HAI score and MPO activity. IgM, IgG and IgA levels were significantly reduced in colon mucus, and the number of Tfh cells was markedly decreased in MLN after VSL#3 treatment. It was concluded that VSL#3 alleviates DSS-induced colitis by downregulating Tfh cells, and Tfh cells may become a potential therapeutic target for IBD. 展开更多
关键词 t FOLliCULAR HELPER cells PROBIOtICS VSL#3 HUMORAL immunity inflammatory BOWEL disease
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Differentially expressed genes and signalling pathways are involved in mouse osteoblast-like MC3T3-E1 cells exposed to 17-β estradiol 被引量:2
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作者 Zhen-Zhen Shang Xin Li +3 位作者 Hui-Qiang Sun Guo-Ning Xiao Cun-Wei Wang Qi Gong 《International Journal of Oral Science》 SCIE CAS CSCD 2014年第3期142-149,共8页
Oestrogen is essential for maintaining bone mass, and it has been demonstrated to induce osteoblast proliferation and bone formation.In this study, complementary DNA(cDNA) microarrays were used to identify and study... Oestrogen is essential for maintaining bone mass, and it has been demonstrated to induce osteoblast proliferation and bone formation.In this study, complementary DNA(cDNA) microarrays were used to identify and study the expression of novel genes that may be involved in MC3T3-E1 cells’ response to 17-b estradiol. MC3T3-E1 cells were inoculated in minimum essential media alpha(a-MEM)cell culture supplemented with 17-b estradiol at different concentrations and for different time periods. MC3T3-E1 cells treated with1028mol?L2117-b estradiol for 5 days exhibited the highest proliferation and alkaline phosphatase(ALP) activity; thus, this group was chosen for microarray analysis. The harvested RNA was used for microarray hybridisation and subsequent real-time reverse transcription polymerase chain reaction(RT-PCR) to validate the expression levels for selected genes. The microarray results were analysed using both functional and pathway analysis. In this study, microarray analysis detected 5 403 differentially expressed genes,of which 1 996 genes were upregulated and 3 407 genes were downregulated, 1 553 different functional classifications were identified by gene ontology(GO) analysis and 53 different pathways were involved based on pathway analysis. Among the differentially expressed genes, a portion not previously reported to be associated with the osteoblast response to oestrogen was identified. These findings clearly demonstrate that the expression of genes related to osteoblast proliferation, cell differentiation, collagens and transforming growth factor beta(TGF-b)-related cytokines increases, while the expression of genes related to apoptosis and osteoclast differentiation decreases, following the exposure of MC3T3-E1 cells to a-MEM supplemented with 17-b estradiol. Microarray analysis with functional gene classification is critical for a complete understanding of complementary intracellular processes. This microarray analysis provides large-scale gene expression data that require further confirmatory studies. 展开更多
关键词 17-β estradiol MC3t3-E1 cell MICROARRAY signal transduction
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Overexpression of 14-3-3 protein protects pheochromocytoma cells against 1-methyl-4-phenylpyridinium toxicity 被引量:1
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作者 陈小武 孙圣刚 +1 位作者 称道宾 田有勇 《Neuroscience Bulletin》 SCIE CAS CSCD 2006年第5期281-287,共7页
Objective To investigate the effects of 14-3-3 protein overexpression on the 1-methyl-4-phenylpyridinium (MPP^+) induced pheochromocytoma (PC12) cell death and the potential mechanisms. Methods pcDNA3.1(+)-14-... Objective To investigate the effects of 14-3-3 protein overexpression on the 1-methyl-4-phenylpyridinium (MPP^+) induced pheochromocytoma (PC12) cell death and the potential mechanisms. Methods pcDNA3.1(+)-14-3-3 plasmids, which could be expressed in mammalian cell, were constructed and transfected into PC 12 cells with Lipofectamine 2000. The expression of 14-3-3 protein, Bcl-2 protein, and BAD protein were determined by western blot. 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, microplate reader, and flow cytometric analysis were used to measure cell viability, the caspase activity, and apoptotic ratio respectively. Results (1) The expression of 14-3-3 protein increased significantly three weeks after pcDNA3.1(+)-14-3-3 plasmids transfected into PC 12 cells. (2) MPP^+ caused a decrease of cell viability in a dose-dependent manner. At 100μmol/L MPP^+, cell viability reduced approximately 50%. (3) The caspase activity increased along with the MPP^+ concentrations rising and reached its maximum value (0.34 μmol/mg protein) at 100 μmol/L MPP*. However caspase activity decreased significantly when the MPP^+ concentration exceeded 100 μmol/L. (4) Overexpression of 14-3-3 protein decreased the apoptosis ratio of PC 12 cells treated with 100μmol/L MPP^+ from 26.5% to 8.6%. (5) Bcl-2 protein tended to decrease but BAD protein tended to increase after treatment of PC 12 cells with 100 μmol/L MPP^+. Overexpression of 14-3-3 protein significantly increased the cellular level of Bcl-2 protein and decreased that of BAD protein. Conclusion Overexpression of 14-3-3 protein may reduce MPP^+-induced apoptotic cell death in PC12 cells by up-regulating the Bcl-2 expression and down-regulating the BAD expression. These results may provide a promising target for treatment of Parkinson's disease. 展开更多
关键词 14-3-3 protein MPP^+ PC 12 cell apoptosis Parkinson's disease
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Cr-poisoning under open-circuit condition in LaNi_(0.6)Fe_(0.4)O_(3-δ)-based nano composite cathodes for solid oxide fuel cells prepared by infiltration process 被引量:1
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作者 Yeong-Ju CHOE Jeong-Uk SEO +2 位作者 Kyoung-Jin LEE Min-Jin LEE Hae-Jin HWANG 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2016年第5期1367-1372,共6页
LaNi(0.6)Fe(0.4)O(3-δ) (LNF) powders were synthesized by the glycine-nitrate process and LNF-gadolinium-doped ceria (GDC) nanocomposite cathodes for solid oxide fuel cells (SOFCs) were fabricated by infil... LaNi(0.6)Fe(0.4)O(3-δ) (LNF) powders were synthesized by the glycine-nitrate process and LNF-gadolinium-doped ceria (GDC) nanocomposite cathodes for solid oxide fuel cells (SOFCs) were fabricated by infiltration from LNF porous backbones. Electrochemical properties and Cr-poisoning behavior of LNF-GDC cathodes were studied. Single phase perovskite LNF could be obtained at the glycine to nitrate molar ratio of 1:1. The polarization resistance of the LNF-GDC nanocomposite cathode was significantly decreased in comparison with the LNF. This phenomenon was associated with enhanced catalytic activity and enlarged triple-phase boundary (TPB) length by GDC nano particles. In addition, the nanocomposite cathode showed good Cr tolerance under open circuit condition. The LNF-GDC nanocomposite cathodes were expected for use as a potential cathode in intermediate- temperature solid oxide fuel cells (IT-SOFC). 展开更多
关键词 Cr-poisoning PEROVSKItE INFILtRAtION solid oxide fuel cell LaNi0.6Fe0.4O3-δ
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<i>Trapa japonica</i>Flerov Extract Attenuates Lipid Accumulation through Downregulation of Adipogenic Transcription Factors in 3T3-L1 Cells 被引量:1
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作者 Mi Jin Kim Kyung Ran Im Kyung-Sup Yoon 《American Journal of Molecular Biology》 2015年第2期32-41,共10页
Obesity is a major human health problem associated with various diseases, including cardiac injury and type 2 diabetes. Trapa japonica Flerov (TJF) has been used in traditional oriental medicine to treat diabetes. In ... Obesity is a major human health problem associated with various diseases, including cardiac injury and type 2 diabetes. Trapa japonica Flerov (TJF) has been used in traditional oriental medicine to treat diabetes. In this study, we evaluated the inhibitory effect of and the mechanism underlying the effect of TJF extract on adipogenesis in 3T3-L1 cells. The effects of TJF extract on cell viability were analyzed using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, and the anti-adipogenic effect was measured by oil red O staining. The expression of peroxisomal proliferator activated receptor (PPAR)γ, CCAAT/enhancer-binding protein-α (C/EBP)α, adenosine monophosphate-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), adiponectin, and fatty acid binding protein (FABP)4 involved in adipogenesis was determined by western blot analysis. TJF extract effectively inhibited lipid accumulation and the expression of PPARγ and C/EBPα in 3T3-L1 cells. TJF also increased the phosphorylation of AMPK and ACC, and decreased the expression of adiponectin and FABP4. These results indicate that TJF extract exerts its anti-obesity effect through the downregulation of adipogenic transcription factors and adipogenic marker genes. 展开更多
关键词 3t3-L1 cells Adipogenic transcription Factors lipid Accumulation tRAPA JAPONICA Flerov EXtRACt
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MiR-494-3p regulates cell proliferation and apoptosis via KLF7 in Schwann cells 被引量:1
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作者 YANGUO PENG WEI LI +2 位作者 SHUIYU CHEN WANGCHUN WU ZHANFANG SHANG 《BIOCELL》 SCIE 2021年第1期57-64,共8页
Peripheral nerve injury is a common neurodegenerative disease,which causes disability and a huge economic burden for patients.MicroRNAs(miRNAs)have been acknowledged as major regulators and therapeutic targets of neur... Peripheral nerve injury is a common neurodegenerative disease,which causes disability and a huge economic burden for patients.MicroRNAs(miRNAs)have been acknowledged as major regulators and therapeutic targets of neurological disease.Thus,the functional studies of miRNAs in neurological disease will contribute to discover new therapeutic targets for peripheral nerve injury.Sprague Dawley rats treated sciatic nerve surgical injury were regarded as peripheral nerve injury model in vivo.The expression of miR-494-3p and Kruppel like factor7(KLF7)were measured by Real-time quantitative polymerase chain reaction(RT-qPCR)assay.In addition,western blot analysis was conducted to measure the protein levels of KLF7,Bax,Bcl-2,and C-caspase 3.Cell viability and apoptosis were detected in Schwann cells by EdU stain and flow cytometry,respectively.The interaction between miR-494-3p and KLF7 was investigated by dual-luciferase reporter assay.The expression of miR-494-3p was reduced at the beginning,but KLF7 was enhanced in Sprague Dawley rats with peripheral nerve injury.Knockdown of miR-494-3p promoted cell proliferation and suppressed apoptosis,while overexpression of miR-494-3p or silencing KLF7 led to opposite results.Moreover,the upregulation of KLF7 attenuated miR-494-3p overexpression-induced suppressive effects on viability and promotion of apoptosis in Schwann cells.MiR-494-3p negatively regulates KLF7 in Schwann cells to mediate proliferation and apoptosis. 展开更多
关键词 Peripheral nerve injury miR-494-3p KLF7 Schwann cells
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La_(0.75)Sr_(0.25)Cr_(0.5)Mn_(0.5)O_(3)-δ−Ce_(0.8)Gd_(0.2)O_(1.9) composite electrodes as anodes in LaGaO_(3)-based direct carbon solid oxide fuel cells 被引量:2
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作者 CHEN Tian-yu XIE Yong-min +7 位作者 LU Zhi-bin WANG Liang CHEN Zhe-qin ZHONG Xiao-cong LIU Jia-ming WANG Rui-xiang XU Zhi-feng OUYANG Shao-bo 《Journal of Central South University》 SCIE EI CAS CSCD 2022年第6期1788-1798,共11页
Direct carbon solid oxide fuel cells(DC-SOFCs)are promising,green,and efficient power-generating devices that are fueled by solid carbons and comprise all-solid-state structures.Developing suitable anode materials for... Direct carbon solid oxide fuel cells(DC-SOFCs)are promising,green,and efficient power-generating devices that are fueled by solid carbons and comprise all-solid-state structures.Developing suitable anode materials for DC-SOFCs is a substantial scientific challenge.Herein we investigated the use of La_(0.75)Sr_(0.25)Cr_(0.5)Mn_(0.5)O_(3)-δ−Ce_(0.8)Gd_(0.2)O_(1.9)(LSCM−GDC)composite electrodes as anodes for La_(0.9)Sr_(0.1)Ga_(0.8)Mg_(0.2)O_(3)-δelectrolyte-based DC-SOFCs,with Camellia oleifera shell char as the carbon fuel.The LSCM−GDC-anode DC-SOFC delivered a maximum power density of 221 mW/cm^(2) at 800℃ and it significantly improved to 425 mW/cm^(2) after Ni nanoparticles were introduced into the LSCM−GDC anode through wet impregnation.The microstructures of the prepared anodes were characterized,and the stability of the anode in a DC-SOFC and the influence of catalytic activity on open circuit voltage were studied.The above results indicate that LSCM–GDC anode is promising to be applied in DC-SOFCs. 展开更多
关键词 direct carbon solid oxide fuel cells anode material La_(0.75)Sr_(0.25)Cr_(0.5)Mn_(0.5)O_(3)-δ−Ce_(0.8)Gd_(0.2)O_(1.9) composite electrodes Ni nanoparticles
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Effects of miR-214-5p and miR-21-5p in hypoxic endometrial epithelial-cell-derived exosomes on human umbilical cord mesenchymal stem cells
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作者 Wan-Yu Zhang Han-Bi Wang Cheng-Yan Deng 《World Journal of Stem Cells》 SCIE 2024年第11期906-925,共20页
BACKGROUND Thin endometrium seriously affects endometrial receptivity,resulting in a significant reduction in embryo implantation,and clinical pregnancy and live birth rates,and there is no gold standard for treatment... BACKGROUND Thin endometrium seriously affects endometrial receptivity,resulting in a significant reduction in embryo implantation,and clinical pregnancy and live birth rates,and there is no gold standard for treatment.The main pathophysiological characteristics of thin endometrium are increased uterine arterial blood flow resistance,angiodysplasia,slow growth of the glandular epithelium,and low expression of vascular endothelial growth factor,resulting in endometrial epithelial cell(EEC)hypoxia and endometrial tissue aplasia.Human umbilical cord mesenchymal stem cells(HucMSCs)promote repair and regeneration of damaged endometrium by secreting microRNA(miRNA)-carrying exosomes.However,the initiation mechanism of HucMSCs to repair thin endometrium has not yet been clarified.AIM To determine the role of hypoxic-EEC-derived exosomes in function of HucMSCs and explore the potential mechanism.METHODS Exosomes were isolated from normal EECs(EEC-exs)and hypoxia-damaged EECs(EECD-exs),before characterization using Western blotting,nanoparticletracking analysis,and transmission electron microscopy.HucMSCs were cocultured with EEC-exs or EECD-exs and differentially expressed miRNAs were determined using sequencing.MiR-21-5p or miR-214-5p inhibitors or miR-21-3p or miR-214-5p mimics were transfected into HucMSCs and treated with a signal transducer and activator of transcription 3(STAT3)activator or STAT3 inhibitor.HucMSC migration was assessed by Transwell and wound healing assays.Differentiation of HucMSCs into EECs was assessed by detecting markers of stromal lineage(Vimentin and CD13)and epithelial cell lineage(CK19 and CD9)using Western blotting and immunofluorescence.The binding of the miRNAs to potential targets was validated by dual-luciferase reporter assay.RESULTS MiR-21-5p and miR-214-5p were lowly expressed in EECD-ex-pretreated HucMSCs.MiR-214-5p and miR-21-5p inhibitors facilitated the migratory and differentiative potentials of HucMSCs.MiR-21-5p and miR-214-5p targeted STAT3 and protein inhibitor of activated STAT3,respectively,and negatively regulated phospho-STAT3.MiR-21-5p-and miR-214-5p-inhibitor-induced promotive effects on HucMSC function were reversed by STAT3 inhibition.MiR-21-5p and miR-214-5p overexpression repressed HucMSC migration and differentiation,while STAT3 activation reversed these effects.CONCLUSION Low expression of miR-21-5p/miR-214-5p in hypoxic-EEC-derived exosomes promotes migration and differentiation of HucMSCs into EECs via STAT3 signaling.Exosomal miR-214-5p/miR-21-5p may function as valuable targets for thin endometrium. 展开更多
关键词 Endometrial epithelial cells EXOSOMES Human umbilical cord mesenchymal stem cells MiR-214-5p/miR-21-5p Signal transducer and activator of transcription 3
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Involvement of ERK1/2 and p38 MAPK in up-regulation of 14-3-3 protein induced by hydrogen peroxide preconditioning in PC12 cells
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作者 苏庆杰 陈小武 +1 位作者 陈志斌 孙圣刚 《Neuroscience Bulletin》 SCIE CAS CSCD 2008年第4期244-250,共7页
Objective To investigate the protective effects of hydrogen peroxide preconditioning (HPP) on the pheochromocytoma (PC12) cells treated with 1-methyl-4-phenylpyridinium (MPP^+) and to explore the potential mech... Objective To investigate the protective effects of hydrogen peroxide preconditioning (HPP) on the pheochromocytoma (PC12) cells treated with 1-methyl-4-phenylpyridinium (MPP^+) and to explore the potential mechanisms. Methods The viability and apoptosis of PC 12 cells were determinded by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and 4′,6′-diamidino-2-phenylindole (DAPI) staining, respectively. The expressions of 14-3-3 protein and phospholylated p38 mitogen-activated protein kinase (MAPK) were determined by Western blot. Enzyme-linked immunosorbent assay (ELISA) was used to measure the activity of extracellular signal-regulated protein kinase 1/2 (ERK1/2). Results The cell viability decreased and the number of apoptotic cells increased dramatically in MPP^+ group compared with that in Control group. HPP induced a significant increase in cell viability and a marked decrease in population of apoptotic cells of the MPP^+- treated PC 12 cells, accompanied with up-regulation of 14-3-3 protein and increase of ERK 1/2 and p38 MAPK activities. The 14-3-3 protein expression was positively correlated with the phosphorylation of ERK1/2. Furthermore, inhibition of the ERK1/2 with PD98059 abolished the 14-3-3 protein up-regulation in PC 12 cells induced by HPP. Conclusion HPP protects PC 12 cells against MPP+ toxicity by up-regulating 14-3-3 protein expression through the ERK1/2 and p38 MAPK signaling pathways. 展开更多
关键词 hydrogen peroxide preconditioning 14-3-3 protein ERK1/2 p38 mitogen-activated protein kinase PC12 cell
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Curcumin Inhibits Adipogenesis Elicited by Clozapine in 3T3-L1 Cells
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作者 Satoru Sakuma Maki Sumida +7 位作者 Asami Sakabe Ayaka Nakamura Chisato Noda Kaho Tsujimoto Maimi Kobayashi Tomohiko Sano Yohko Fujimoto Tetsuya Kohda 《Food and Nutrition Sciences》 2018年第5期584-594,共11页
Although clozapine (CZP), which is used for schizophrenia treatment, causes weight gain, the mechanism remains unclear. We recently reported that the naturally occurring compound curcumin (CUR) suppresses adipogenesis... Although clozapine (CZP), which is used for schizophrenia treatment, causes weight gain, the mechanism remains unclear. We recently reported that the naturally occurring compound curcumin (CUR) suppresses adipogenesis in 3T3-L1 cells. The aims of the present study were to determine the mechanism by which CZP induces adipocyte differentiation of 3T3-L1 cells, and whether CUR reduces CZP-induced adipogenesis. We found that cells grown in the presence of CZP had significantly higher triacylglycerol levels, numbers of lipid-filled adipocytes, and mRNA expression levels of CCAAT-enhancer binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ) than those grown without CZP. Treatment with CZP plus CUR resulted in major reductions in these four parameters. These results suggest that CZP enhances adipogenesis in 3T3-L1 cells via the C/EBPα-PPARγ pathway and that by interrupting CZP’s effects, CUR might be a potent agent for preventing CZP-induced weight gain. 展开更多
关键词 CLOZAPINE CURCUMIN ADIPOCYtE Differentiation 3t3-L1 cell Atypical ANtIPSYCHOtIC Drug
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