Near-field 3D imaging approach combining MJSR and FGG-NUFFT

A near-field three-dimensional(3 D)imaging method combining multichannel joint sparse recovery(MJSR)and fast Gaussian gridding nonuniform fast Fourier transform(FGGNUFFT)is proposed,based on a perfect combination of the compressed sensing(CS)theory and the matched filtering(MF)technique.The approach has the advantages of high precision and high efficiency:multichannel joint sparse constraint is adopted to improve the problem that the images recovered by the single channel imaging algorithms do not necessarily share the same positions of the scattering centers;the CS dictionary is constructed by combining MF and FGG-NUFFT,so as to improve the imaging efficiency and memory requirement.Firstly,a near-field 3 D imaging model of joint sparse recovery is constructed by combining the MF-based imaging method.Secondly,FGG-NUFFT and reverse FGG-NUFFT are used to replace the interpolation and Fourier transform in MF-based imaging methods,and a sensing matrix with high precision and high efficiency is constructed according to the traditional imaging process.Thirdly,a fast imaging recovery is performed by using the improved separable surrogate functionals(SSF)optimization algorithm,only with matrix and vector multiplication.Finally,a 3 D imagery of the near-field target is obtained by using both the horizontal and the pitching interferometric phase information.This paper contains two imaging models,the only difference is the sub-aperture method used in inverse synthetic aperture radar(ISAR)imaging.Compared to traditional CS-based imaging methods,the proposed method includes both forward transform and inverse transform in each iteration,which improves the quality of reconstruction.The experimental results show that,the proposed method improves the imaging accuracy by about O(10),accelerates the imaging speed by five times and reduces the memory usage by about O(10~2).

3D imaging lipidometry in single cell by in-flow holographic tomography

The most recent discoveries in the biochemical field are highlighting the increasingly important role of lipid droplets(LDs)in several regulatory mechanisms in living cells.LDs are dynamic organelles and therefore their complete characterization in terms of number,size,spatial positioning and relative distribution in the cell volume can shed light on the roles played by LDs.Until now,fluorescence microscopy and transmission electron microscopy are assessed as the gold standard methods for identifying LDs due to their high sensitivity and specificity.However,such methods generally only provide 2D assays and partial measurements.Furthermore,both can be destructive and with low productivity,thus limiting analysis of large cell numbers in a sample.Here we demonstrate for the first time the capability of 3D visualization and the full LD characterization in high-throughput with a tomographic phase-contrast flow-cytometer,by using ovarian cancer cells and monocyte cell lines as models.A strategy for retrieving significant parameters on spatial correlations and LD 3D positioning inside each cell volume is reported.The information gathered by this new method could allow more in depth understanding and lead to new discoveries on how LDs are correlated to cellular functions.

Monopulse instantaneous 3D imaging for wideband radar system

To avoid the complicated motion compensation in interferometric inverse synthetic aperture(InISAR)and achieve realtime three-dimensional(3 D)imaging,a novel approach for 3 D imaging of the target only using a single echo is presented.This method is based on an isolated scatterer model assumption,thus the scatterers in the beam can be extracted individually.The radial range of each scatterer is estimated by the maximal likelihood estimation.Then,the horizontal and vertical wave path difference is derived by using the phase comparison technology for each scatterer,respectively.Finally,by utilizing the relationship among the 3 D coordinates,the radial range,the horizontal and vertical wave path difference,the 3 D image of the target can be reconstructed.The reconstructed image is free from the limitation in InISAR that the image plane depends on the target's own motions and on its relative position with respect to the radar.Furthermore,a phase ambiguity resolution method is adopted to ensure the success of the 3 D imaging when phase ambiguity occurs.It can be noted that the proposed phase ambiguity resolution method only uses one antenna pair and does not require a priori knowledge,whereas the existing phase ambiguity methods may require two or more antenna pairs or a priori knowledge for phase unwarping.To evaluate the performance of the proposed method,the theoretical analyses on estimation accuracy are presented and the simulations in various scenarios are also carried out.

Airborne sparse flight array SAR 3D imaging based on compressed sensing in frequency domain

In airborne array synthetic aperture radar(SAR), the three-dimensional(3D) imaging performance and cross-track resolution depends on the length of the equivalent array. In this paper, Barker sequence criterion is used for sparse flight sampling of airborne array SAR, in order to obtain high cross-track resolution in as few times of flights as possible. Under each flight, the imaging algorithm of back projection(BP) and the data extraction method based on modified uniformly redundant arrays(MURAs) are utilized to obtain complex 3D image pairs. To solve the side-lobe noise in images, the interferometry between each image pair is implemented, and compressed sensing(CS) reconstruction is adopted in the frequency domain. Furthermore, to restore the geometrical relationship between each flight, the phase information corresponding to negative MURA is compensated on each single-pass image reconstructed by CS. Finally,by coherent accumulation of each complex image, the high resolution in cross-track direction is obtained. Simulations and experiments in X-band verify the availability.

3D Depth Measurement for Holoscopic 3D Imaging System

Holoscopic 3D imaging is a true 3D imaging system mimics fly’s eye technique to acquire a true 3D optical model of a real scene. To reconstruct the 3D image computationally, an efficient implementation of an Auto-Feature-Edge (AFE) descriptor algorithm is required that provides an individual feature detector for integration of 3D information to locate objects in the scene. The AFE descriptor plays a key role in simplifying the detection of both edge-based and region-based objects. The detector is based on a Multi-Quantize Adaptive Local Histogram Analysis (MQALHA) algorithm. This is distinctive for each Feature-Edge (FE) block i.e. the large contrast changes (gradients) in FE are easier to localise. The novelty of this work lies in generating a free-noise 3D-Map (3DM) according to a correlation analysis of region contours. This automatically combines the exploitation of the available depth estimation technique with edge-based feature shape recognition technique. The application area consists of two varied domains, which prove the efficiency and robustness of the approach: a) extracting a set of setting feature-edges, for both tracking and mapping process for 3D depthmap estimation, and b) separation and recognition of focus objects in the scene. Experimental results show that the proposed 3DM technique is performed efficiently compared to the state-of-the-art algorithms.

Whole-tissue 3D imaging reveals intra-adipose sympathetic plasticity regulated by NGF-TrkA signal in cold-induced beiging

Sympathetic arborizations act as the essential efferent signals in regulating the metabolism of peripheral organs including white adipose tissues （WAT）. However, whether these local neural structures would be of plastic nature, and how such plasticity might participate in specific metabolic events of WAT, remains largely uncharacterized. In this study, we exploit the new vol- ume fluorescence-imaging technique to observe the significant, and also reversible, plasticity of intra-adi- pose sympathetic arborizations in mouse inguinal WAT in response to cold challenge. We demonstrate that this sympathetic plasticity depends on the cold-elicited sig- nal of nerve growth factor （NGF） and TrkA receptor. Blockage of NGF or TrkA signaling suppresses intra- adipose sympathetic plasticity, and moreover, the cold- induced beiging process of WAT. Furthermore, we show that NGF expression in WAT depends on the cate- cholamine signal in cold challenge. We therefore reveal the key physiological relevance, together with the reg- ulatory mechanism, of intra-adipose sympathetic plas- ticity in the WAT metabolism.

Evaluating the morphology of the degradation layer of pure magnesium via 3D imaging at resolutions below 40 nm

Magnesium is attractive for the application as a temporary bone implant due to its inherent biodegradability,non-toxicity and suitable mechanical properties.The degradation process of magnesium in physiological environments is complex and is thought to be a diffusion-limited transport problem.We use a multi-scale imaging approach using micro computed tomography and transmission X-ray microscopy(TXM)at resolutions below 40 nm.Thus,we are able to evaluate the nanoporosity of the degradation layer and infer its impact on the degradation process of pure magnesium in two physiological solutions.Magnesium samples were degraded in simulated body fluid(SBF)or Dulbecco’s modified Eagle’s medium(DMEM)with 10%fetal bovine serum(FBS)for one to four weeks.TXM reveals the three-dimensional interconnected pore network within the degradation layer for both solutions.The pore network morphology and degradation layer composition are similar for all samples.By contrast,the degradation layer thickness in samples degraded in SBF was significantly higher and more inhomogeneous than in DMEM+10%FBS.Distinct features could be observed within the degradation layer of samples degraded in SBF,suggesting the formation of microgalvanic cells,which are not present in samples degraded in DMEM+10%FBS.The results suggest that the nanoporosity of the degradation layer and the resulting ion diffusion processes therein have a limited influence on the overall degradation process.This indicates that the influence of organic components on the dampening of the degradation rate by the suppression of microgalvanic degradation is much greater in the present study.

High-speed 3D imaging based on structured illumination and electrically tunable lens

In this Letter, we present a high-speed volumetric imaging system based on structured illumination and an electrically tunable lens（ETL）, where the ETL performs fast axial scanning at hundreds of Hz. In the system,a digital micro-mirror device（DMD） is utilized to rapidly generate structured images at the focal plane in synchronization with the axial scanning unit. The scanning characteristics of the ETL are investigated theoretically and experimentally. Imaging experiments on pollen samples are performed to verify the optical cross-sectioning and fast axial scanning capabilities. The results show that our system can perform fast axial scanning and threedimensional（3D） imaging when paired with a high-speed camera, presenting an economic solution for advanced biological imaging applications.

Structured-Light-Field 3D Imaging System With Coaxial Projection

This paper presents a novel 3D measurement method for a light field camera(LFC)in which 3D information of object space is encoded by a microlens array(MLA).The light ray corresponding to each pixel of the LFC is calibrated.Once the matching points from at least two subviews exhibit sub-pixel accuracy,the 3D coordinates can be calculated optimally by intersecting light rays of these points matched through phase coding.Moreover,the proposed method obtains high-resolved results that exceed the subview resolution due to the virtual continuous phase search strategy.Finally,we combine the LFC and coaxial projection to solve the 3D data loss caused by shadowing and occlusion problems.Experimental results verify the feasibility of the proposed method,and the measurement error is about 30μm in a depth range of 60 mm.

Development and prospect of acoustic reflection imaging logging processing and interpretation method

Acoustic reflection imaging logging technology can detect and evaluate the development of reflection anomalies,such as fractures,caves and faults,within a range of tens of meters from the wellbore,greatly expanding the application scope of well logging technology.This article reviews the development history of the technology and focuses on introducing key methods,software,and on-site applications of acoustic reflection imaging logging technology.Based on the analyses of major challenges faced by existing technologies,and in conjunction with the practical production requirements of oilfields,the further development directions of acoustic reflection imaging logging are proposed.Following the current approach that utilizes the reflection coefficients,derived from the computation of acoustic slowness and density,to perform seismic inversion constrained by well logging,the next frontier is to directly establish the forward and inverse relationships between the downhole measured reflection waves and the surface seismic reflection waves.It is essential to advance research in imaging of fractures within shale reservoirs,the assessment of hydraulic fracturing effectiveness,the study of geosteering while drilling,and the innovation in instruments of acoustic reflection imaging logging technology.

Stimulated Raman scattering microscopy with phase-controlled light focusing and aberration correction for rapid and label-free, volumetric deep tissue imaging

We report a novel stimulated Raman scattering(SRS)microscopy technique featuring phase-controlled light focusing and aberration corrections for rapid,deep tissue 3D chemical imaging with subcellular resolution.To accomplish phasecontrolled SRS(PC-SRS),we utilize a single spatial light modulator to electronically tune the axial positioning of both the shortened-length Bessel pump and the focused Gaussian Stokes beams,enabling z-scanning-free optical sectioning in the sample.By incorporating Zernike polynomials into the phase patterns,we simultaneously correct the system aberrations at two separate wavelengths(~240 nm difference),achieving a~3-fold enhancement in signal-to-noise ratio over the uncorrected imaging system.PC-SRS provides>2-fold improvement in imaging depth in various samples(e.g.,polystyrene bead phantoms,porcine brain tissue)as well as achieves SRS 3D imaging speed of~13 Hz per volume for real-time monitoring of Brownian motion of polymer beads in water,superior to conventional point-scanning SRS 3D imaging.We further utilize PC-SRS to observe the metabolic activities of the entire tumor liver in living zebrafish in cellsilent region,unraveling the upregulated metabolism in liver tumor compared to normal liver.This work shows that PCSRS provides unprecedented insights into morpho-chemistry,metabolic and dynamic functioning of live cells and tissue in real-time at the subcellular level.

The diagnostic value of 3D spiral CT imaging of cholangiopancreatic ducts on obstructive jaundice

Objective: Computerized tomography (CT) plays an important role in the diagnosis of diseases of biliary tract. Recently, three dimensions (3D) spiral CT imaging has been used in surgical diseases gradually. This study was designed to evaluate the diagnostic value of 3D spiral CT imaging of cholangiopancreatic ducts on obstructive jaundice. Methods: Thirty patients with obstructive jaundice had received B-mode ultrasonography, CT, percutaneous transhepatic cholangiography (PTC) or endoscopic retrograde cholangiopancreatography (ERCP), and 3D spiral CT imaging of cholangiopancreatic ducts preoperatively. Then the diagnose accordance rate of these examinational methods were compared after operations. Results: The diagnose accordance rate of 3D spiral CT imaging of cholangiopancreatic ducts was higher than those of B-mode ultrasonography, CT, or single PTC or ERCP, which showed clear images of bile duct tree and pathological changes. As to malignant obstructive jaundice, this examinational technique could clearly display the adjacent relationship between tumor and liver tissue, biliary ducts, blood vessels, and intrahepatic metastases. Conclusion: 3D spiral CT imaging of cholangiopancreatic ducts has significant value for obstructive diseases of biliary ducts, which provides effective evidence for the feasibility of tumor-resection and surgical options.

Reduced Imaging Time and Improved Image Quality of 3D Isotropic T2-Weighted Magnetic Resonance Imaging with Compressed Sensing for the Female Pelvis

This study is to compare three-dimensional(3D)isotropic T2-weighted magnetic resonance imaging(MRI)with compressed sensing-sampling perfection with application optimized contrast(CS-SPACE)and the conventional image(3D-SPACE)sequence in terms of image quality,estimated signal-to-noise ratio(SNR),relative contrast-to-noise ratio(CNR),and the lesions’conspicuous of the female pelvis.Thirty-six females(age:51,28-73)with cervical carcinoma(n=20),rectal carcinoma(n=7),or uterine fibroid(n=9)were included.Patients underwent magnetic resonance(MR)imaging at a 3T scanner with the sequences of 3D-SPACE,CS-SPACE,and twodimensional(2D)T2-weighted turbo-spin echo(TSE).Quantitative analyses of estimated SNR and relative CNR between tumors and other tissues,image quality,and tissue conspicuity were performed.Two radiologists assessed the difference in diagnostic findings for carcinoma.Quantitative values and qualitative scores were analyzed,respectively.The estimated SNR and the relative CNR of tumor-to-muscle obturator internus,tumor-to-myometrium,and myometrium-to-muscle obturator internus was comparable between 3D-SPACE and CS-SPACE.The overall image quality and the conspicuity of the lesion scores of the CS-SPACE were higher than that of the 3D-SPACE(P<0.01).The CS-SPACE sequence offers shorter scan time,fewer artifacts,and comparable SNR and CNR to conventional 3D-SPACE,and has the potential to improve the performance of T2-weighted images.

3D Confocal Raman Imaging of Oil-Rich Emulsion from Enzyme-Assisted Aqueous Extraction of Extruded Soybean Powder

The understanding of the structure morphology of oil-rich emulsion from enzyme-assisted extraction processing(EAEP)was a critical step to break the oil-rich emulsion structure in order to recover oil.Albeit EAEP method has been applied as an alternative way to conventional solvent extraction method,the structure morphology of oil-rich emulsion was still unclear.The current study aimed to investigate the structure morphology of oil-rich emulsion from EAEP using 3 D confocal Raman imaging technique.With increasing the enzymatic hydrolysis duration from 1 to 3 h,the stability of oil-rich emulsion was decreased as visualized in the 3 D confocal Raman images that the protein and oil were mixed together.The subsequent Raman spectrum analysis further revealed that the decreased stability of oil-rich emulsion was due to the protein aggregations via SS bonds or protein-lipid interactions.The conformational transfer in protein indicated the formation of a compact structure.

The Application of Digital Optical 3D Image Analyzer Evaskin in The Evaluation of Wrinkles

To verify the effectiveness of digital optical 3D image analyzer EvaSKIN in the objective and quantitative evaluation of wrinkles.A total of 115 subjects were recruited,the facial images of the subjects were collected by digital optical 3D image analyzer and manual camera,the changes of crow’s feet with age were analyzed.Pictures obtained by manual photography can be directly used for observation and preliminary grading of wrinkles.However,the requirements for evaluators are high,and the results are prone to errors,which will affect the accuracy of the evaluation.Therefore,skilled raters are needed.Compared with the manual photography method,the digital optical 3D image analyzer EvaSKIN can realize three-dimensional extraction of wrinkles,and obtain the change trend of crow’s feet with age.20~30 years old,wrinkles begin to appear slowly;wrinkles will increase rapidly at the age of 30~50;The length of 50~60 year old wrinkles is basically fixed,the wrinkles develop longitudewise,gradually widen and deepen,and the area,depth and volume increase is obvious,and the skin aging condition is intensified.the digital optical 3D image analyzer EvaSKIN realizes the 3D extraction of wrinkles,quantifies the circumference,area,average depth,maximum depth and volume of wrinkles,realizes the objective and quantitative evaluation of wrinkle state,is more accurate in the measurement of wrinkles,and provides a new instrument and method for the evaluation of wrinkles.it is a perfect and supplement to the traditional evaluation methods,and to a certain extent,it helps the research and development and evaluation institutions of cosmetics to obtain more abundant and three-dimensional data support.

Deep learning enhanced NIR-Ⅱ volumetric imaging of whole mice vasculature

Fluorescence imaging through the second near-infrared window(NIR-Ⅱ,1000–1700 nm) allows in-depth imaging.However, current imaging systems use wide-field illumination and can only provide low-contrast 2D information, without depth resolution. Here, we systematically apply a light-sheet illumination, a time-gated detection, and a deep-learning algorithm to yield high-contrast high-resolution volumetric images. To achieve a large Fo V(field of view) and minimize the scattering effect, we generate a light sheet as thin as 100.5 μm with a Rayleigh length of 8 mm to yield an axial resolution of 220 μm. To further suppress the background, we time-gate to only detect long lifetime luminescence achieving a high contrast of up to 0.45 Icontrast. To enhance the resolution, we develop an algorithm based on profile protrusions detection and a deep neural network and distinguish vasculature from a low-contrast area of 0.07 Icontrast to resolve the 100μm small vessels. The system can rapidly scan a volume of view of 75 × 55 × 20 mm3and collect 750 images within 6mins. By adding a scattering-based modality to acquire the 3D surface profile of the mice skin, we reveal the whole volumetric vasculature network with clear depth resolution within more than 1 mm from the skin. High-contrast large-scale 3D animal imaging helps us expand a new dimension in NIR-Ⅱ imaging.

Design and implementation of GM- APD array readout circuit for infrared imaging

Based on an avalanche photodiode（ APD） detecting array working in Geiger mode（ GM-APD）, a high-performance infrared sensor readout integrated circuit（ ROIC） used for infrared 3D（ three-dimensional） imaging is proposed. The system mainly consists of three functional modules, including active quenching circuit（ AQC）, time-to-digital converter（ TDC） circuit and other timing controller circuit. Each AQC and TDC circuit together constitutes the pixel circuit. Under the cooperation with other modules, the current signal generated by the GM-APD sensor is detected by the AQC, and the photon time-of-flight（ TOF） is measured and converted to a digital signal output to achieve a better noise suppression and a higher detection sensitivity by the TDC. The ROIC circuit is fabricated by the CSMC 0. 5 μm standard CMOS technology. The array size is 8 × 8, and the center distance of two adjacent cells is 100μm. The measurement results of the chip showthat the performance of the circuit is good, and the chip can achieve 1 ns time resolution with a 250 MHz reference clock, and the circuit can be used in the array structure of the infrared detection system or focal plane array（ FPA）.

A computational method for wide-azimuth 3D dip-angle gathers using Gaussian beam migration

The dip-angle-domain common-image gather(DDCIG)is a key tool to separate the diffraction and reflection imaging results.Reflectors with different spatial geometries produce different responses in DDCIGs.Compared with Kirchhoff migration,Gaussian beam migration(GBM)is more effective and robust to overcome the multipathing problem.As a ray-based method,it has explicit angle information naturally during the propagation.We have developed a 3D DDCIG computational method using GBM,which obtain both the imaging result and angle-domain gathers with only one pass of calculation.The angle-gather computation is based on geometrical optics,and multiple angle conversions are implemented under the rules of space geometry,which helps to avoid rounding errors and improve accuracy.Additionally,the multi-azimuth joint presentation strategy is proposed to describe the characteristic of omnidirectional dip angles using a finite number of gathers.After using a 2D model to illustrate application advantages of DDCIG,we apply the proposed method to two 3D models to test its feasibility and accuracy.A field data example further demonstrates the adaptability of our method to seismic imaging for a land survey.

Three-dimensional bistatic interferometric ISA Rimaging

An approach based on interferometry technique is proposed for three-dimensional （3D） bistatic inverse synthetic aperture radar （ISAR） imaging. It is converted to a monostatic problem by using the theory that a bistatic radar equals a monostatic radar located on the bisector of bistatic an- gle. Then, interferometric phases extracted from a pair of cross shaped antennas are used to esti- mate the height and associated rotational velocity. Finally, numerical simulations are provided to e- valuate this method.

Uniaxial experimental study of the acoustic emission and deformation behavior of composite rock based on 3D digital image correlation(DIC)

In this paper, uniaxial compression tests were carried out on a series of composite rock specimens with different dip angles, which were made from two types of rock-like material with different strength. The acoustic emission technique was used to monitor the acoustic signal characteristics of composite rock specimens during the entire loading process. At the same time, an optical non-contact 3 D digital image correlation technique was used to study the evolution of axial strain field and the maximal strain field before and after the peak strength at different stress levels during the loading process. The effect of bedding plane inclination on the deformation and strength during uniaxial loading was analyzed. The methods of solving the elastic constants of hard and weak rock were described. The damage evolution process, deformation and failure mechanism, and failure mode during uniaxial loading were fully determined. The experimental results show that the θ = 0?–45?specimens had obvious plastic deformation during loading, and the brittleness of the θ = 60?–90?specimens gradually increased during the loading process. When the anisotropic angle θincreased from 0?to 90?, the peak strength, peak strain,and apparent elastic modulus all decreased initially and then increased. The failure mode of the composite rock specimen during uniaxial loading can be divided into three categories:tensile fracture across the discontinuities(θ = 0?–30?), slid-ing failure along the discontinuities(θ = 45?–75?), and tensile-split along the discontinuities(θ = 90?). The axial strain of the weak and hard rock layers in the composite rock specimen during the loading process was significantly different from that of the θ = 0?–45?specimens and was almost the same as that of the θ = 60?–90?specimens. As for the strain localization highlighted in the maximum principal strain field, the θ = 0?–30?specimens appeared in the rock matrix approximately parallel to the loading direction,while in the θ = 45?–90?specimens it appeared at the hard and weak rock layer interface.