Oxyphyllones A and B,novel sesquiterpenes with an unusual 4,5-secoeudesmane skeleton from Alpinia oxyphylla

Two novel 4,5-secoeudesmane sesquiterpenoids, oxyphyllones A （1） and B （2） were isolated from the fruits of Alpinia oxyphylla. Their structures were established by spectroscopic methods including 1D and 21） NMR spectra. These two compounds are the first example of naturally occurring sesquiterpenoids with a 4,5-secoeudesmane skeleton in the family of Zingiberaceae and oxyphyllone A （1） is the first 4,5-secoeudesmane type of 13-norsesquiterpenoid. Compounds 1 and 2 exhibited no cytotoxicities against three cancer cell lines at 10 μg/mL. ？ 2009 Ning Hua Tan. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

A Novel Sesquiterpenoid from Coleus xanthanthus

Phytochemical re-investigation of Coleus xanthanthus led to the isolation andidentification of a novel sesquiterpenoid 1, named as 4,5,1 1-trimethyl-8,9-seco-1(10),7(11) eremophiladien-8,12-olid-9-cio acid. The structure of 1 was elucidated by modern spectroscopicmethods, especially by X-ray diffraction.

HPLC法同时测定款冬花中9个主要成分的含量

目的:建立HPLC-DAD法同时测定生药款冬花和蜜炙款冬花中绿原酸、反式咖啡酸、芦丁、金丝桃苷、反式阿魏酸、4,5-O-二咖啡酰基奎宁酸、2,2-二甲基-6-乙酰基苯并二氢吡喃酮、款冬酮和甲基丁酰-3,14-去氢-Z-款冬素酯9个主要成分的含量。方法:采用正交设计优化提取方法,以提取时间、方法、溶剂、溶剂用量为主要影响因素,以9个分析物的总量为评价指标。分析物的色谱分离用Dikma DiamonsilTMC18色谱柱(250 mm×4.6 mm,5μm)、Dikma Easy Guard C18保护柱(20 mm×4.6 mm,5μm);以乙腈(A)-0.03%三氟乙酸水溶液(B)为流动相,梯度洗脱,流速为1.0 mL.min-1;检测波长为240 nm;柱温为25℃。结果:确定最佳提取方法为1.00 g生药样品用20 mL 95%乙醇超声提取1 h。绿原酸、反式咖啡酸、芦丁、金丝桃苷、反式阿魏酸、4,5-O-二咖啡酰基奎宁酸、2,2-二甲基-6-乙酰基苯并二氢吡喃酮、款冬酮和甲基丁酰-3,14-去氢-Z-款冬素酯9个分析物的线性范围分别为62.5～2000μg.mL-1(r=0.9999),2.5～80μg.mL-1(r=0.9994),15.62～500μg.mL-1(r=0.9995),25～800μg.mL-1(r=0.9995),50～1600μg.mL-1(r=0.9992),37.5～1200μg.mL-1(r=0.9994),0.195～12.5μg.mL-1(r=0.9991),15.62～500μg.mL-1(r=0.9991),125～4000μg.mL-1(r=0.9995);平均加样回收率均在95.42%～105.0%范围内,RSD均在0.45%～4.5%范围内。结论:该方法准确,灵敏度高,重复性好,成本低,可用于款冬花中9个主要有效成分的含量测定。