We utilized Raspberry Pi 4B to develop a microbial monitoring system to simplify the microbial image-capturing process and facilitate the informatization of microbial observation results.The Raspberry Pi 4B firmware,d...We utilized Raspberry Pi 4B to develop a microbial monitoring system to simplify the microbial image-capturing process and facilitate the informatization of microbial observation results.The Raspberry Pi 4B firmware,developed under Python on the Linux platform,achieves sum verification of serial data,file upload based on TCP protocol,control of sequence light source and light valve,real-time self-test based on multithreading,and an experiment-oriented file management method.The system demonstrated improved code logic,scheduling,exception handling,and code readability.展开更多
智慧农业是科技创新与农业的融合。在大数据时代,物联网、人工智能和计算机技术的发展,对传统农业的生产和管理方式进行了改革,推动了农业的信息化和智能化。目前,一个待解决的重要问题是如何运用科技手段实时了解农作物的生长状况。在...智慧农业是科技创新与农业的融合。在大数据时代,物联网、人工智能和计算机技术的发展,对传统农业的生产和管理方式进行了改革,推动了农业的信息化和智能化。目前,一个待解决的重要问题是如何运用科技手段实时了解农作物的生长状况。在此介绍了一个以Raspberry Pi 4b为核心控制节点的智能化农作物生长数据采集处理系统。该系统实现了对农作物远程环境的监控和管理,包括对温度、湿度、光照强度和二氧化碳含量等农作物生长必要影响因素的监测。同时,它还实现了对农作物的科学管理和对环境数据的统计分析。展开更多
Dysregulated lineage commitment of mesenchymal stem cells(MSCs)contributes to impaired bone formation and an imbalance between adipogenesis and osteogenesis during skeletal aging and osteoporosis.The intrinsic cellula...Dysregulated lineage commitment of mesenchymal stem cells(MSCs)contributes to impaired bone formation and an imbalance between adipogenesis and osteogenesis during skeletal aging and osteoporosis.The intrinsic cellular mechanism that regulates MSC commitment remains unclear.Here,we identified Cullin 4B(CUL4B)as a critical regulator of MSC commitment.CUL4B is expressed in bone marrow MSCs(BMSCs)and downregulated with aging in mice and humans.Conditional knockout of Cul4b in MSCs resulted in impaired postnatal skeletal development with low bone mass and reduced bone formation.Moreover,depletion of CUL4B in MSCs aggravated bone loss and marrow adipose accumulation during natural aging or after ovariectomy.In addition,CUL4B deficiency in MSCs reduced bone strength.Mechanistically,CUL4B promoted osteogenesis and inhibited adipogenesis of MSCs by repressing KLF4 and C/EBPδexpression,respectively.The CUL4B complex directly bound to Klf4 and Cebpd and epigenetically repressed their transcription.Collectively,this study reveals CUL4B-mediated epigenetic regulation of the osteogenic or adipogenic commitment of MSCs,which has therapeutic implications in osteoporosis.展开更多
文摘We utilized Raspberry Pi 4B to develop a microbial monitoring system to simplify the microbial image-capturing process and facilitate the informatization of microbial observation results.The Raspberry Pi 4B firmware,developed under Python on the Linux platform,achieves sum verification of serial data,file upload based on TCP protocol,control of sequence light source and light valve,real-time self-test based on multithreading,and an experiment-oriented file management method.The system demonstrated improved code logic,scheduling,exception handling,and code readability.
文摘智慧农业是科技创新与农业的融合。在大数据时代,物联网、人工智能和计算机技术的发展,对传统农业的生产和管理方式进行了改革,推动了农业的信息化和智能化。目前,一个待解决的重要问题是如何运用科技手段实时了解农作物的生长状况。在此介绍了一个以Raspberry Pi 4b为核心控制节点的智能化农作物生长数据采集处理系统。该系统实现了对农作物远程环境的监控和管理,包括对温度、湿度、光照强度和二氧化碳含量等农作物生长必要影响因素的监测。同时,它还实现了对农作物的科学管理和对环境数据的统计分析。
基金supported by grants from the National Key R&D Program of China(2022YFC2703700,2022YFC2703701 to Y.G.and 2022YFC2703700,2022YFC2703703 to G.S.)National Natural Science Foundation of China(31872810,82171851 to Y.G.+3 种基金31970559 to B.J.31970781 to G.S.)Key Research and Development Program of Shandong Province(2016GSF201143 to B.J.)Young Scholars Program of Shandong University(to B.J.)。
文摘Dysregulated lineage commitment of mesenchymal stem cells(MSCs)contributes to impaired bone formation and an imbalance between adipogenesis and osteogenesis during skeletal aging and osteoporosis.The intrinsic cellular mechanism that regulates MSC commitment remains unclear.Here,we identified Cullin 4B(CUL4B)as a critical regulator of MSC commitment.CUL4B is expressed in bone marrow MSCs(BMSCs)and downregulated with aging in mice and humans.Conditional knockout of Cul4b in MSCs resulted in impaired postnatal skeletal development with low bone mass and reduced bone formation.Moreover,depletion of CUL4B in MSCs aggravated bone loss and marrow adipose accumulation during natural aging or after ovariectomy.In addition,CUL4B deficiency in MSCs reduced bone strength.Mechanistically,CUL4B promoted osteogenesis and inhibited adipogenesis of MSCs by repressing KLF4 and C/EBPδexpression,respectively.The CUL4B complex directly bound to Klf4 and Cebpd and epigenetically repressed their transcription.Collectively,this study reveals CUL4B-mediated epigenetic regulation of the osteogenic or adipogenic commitment of MSCs,which has therapeutic implications in osteoporosis.
