目的:5-HT1A基因C-1019G多态性与多种神经精神疾病如抑郁症、精神分裂症、焦虑症等的发生风险、症状严重程度及治疗效果存在关联。本研究旨在建立一种直接巢式PCR结合测序检测该位点的方法。方法:以口腔上皮细胞粗处理物为材料,通过直...目的:5-HT1A基因C-1019G多态性与多种神经精神疾病如抑郁症、精神分裂症、焦虑症等的发生风险、症状严重程度及治疗效果存在关联。本研究旨在建立一种直接巢式PCR结合测序检测该位点的方法。方法:以口腔上皮细胞粗处理物为材料,通过直接巢式PCR扩增包含5-HT1A基因C-1019G位点的靶片段,PCR产物经桑格测序鉴定基因型。结果:所检样本均能扩增出预期大小的PCR产物,测序峰图清晰。结论:成功建立了一种直接巢式PCR结合测序鉴定5-HT1A基因型的方法,有良好的应用前景。Objective: The C-1019G polymorphism of the 5-HT1A gene has been associated with the risk of occurrence, symptom severity, and treatment outcome of a variety of neuropsychiatric disorders, such as depression, schizophrenia, and anxiety disorders. The aim of this study was to establish a direct nested PCR combined with sequencing to detect this locus. Methods: The target fragment containing the C-1019G locus of the 5-HT1A gene was amplified by direct nested PCR using crude processed oral epithelial cells, and the PCR product was genotyped by Sanger sequencing. Results: PCR products of expected size were amplified from all the samples tested, and the sequencing peaks were clear. Conclusion: A direct nested PCR combined with sequencing method was successfully established to identify the genotypes of 5-HT1A gene, which has good prospects for application.展开更多
目的:通过观察地黄饮子对PSD大鼠5-羟色胺1A受体(5-HT1AR)与5-羟色胺2A受体(5-HT2 AR)mRNA水平表达的影响,进一步探讨地黄饮子治疗脑卒中后抑郁的可能作用机理。方法:在脑卒中后抑郁大鼠模型上,采用实时荧光定量RT-PCR方法,对PSD大鼠5-H...目的:通过观察地黄饮子对PSD大鼠5-羟色胺1A受体(5-HT1AR)与5-羟色胺2A受体(5-HT2 AR)mRNA水平表达的影响,进一步探讨地黄饮子治疗脑卒中后抑郁的可能作用机理。方法:在脑卒中后抑郁大鼠模型上,采用实时荧光定量RT-PCR方法,对PSD大鼠5-HT1AR与5-HT2 AR mRNA水平进行观察。结果:地黄饮子高、中剂量和百优解均能明显提高PSD大鼠海马5-HT1AR mRNA表达水平、降低5-HT2 AR mRNA表达水平(P<0.05);地黄饮子高、中剂量与百优解无显著差异(P>0.05);地黄饮子低剂量作用不明显。结论:地黄饮子可能是通过上调5-HT1AR mRNA、下调5-HT2 ARmRNA在海马区的表达,达到治疗PSD的目的。展开更多
Objective The ventral part of the medial prefrontal cortex（mPFC）plays an important role in initiation and control of voluntary movement,mood and cognition.However,after the degeneration of the nigrostriatal pathway,...Objective The ventral part of the medial prefrontal cortex（mPFC）plays an important role in initiation and control of voluntary movement,mood and cognition.However,after the degeneration of the nigrostriatal pathway,the neuronal activity of the ventral mPFC and the role of serotonin1A（5-hydroxytryptamine,5-HT1A）receptors in the firing of the neurons are still unknown.The present study is to investigate the change of neuronal activity in the ventral mPFC and the effect of systemic administration of the selective 5-HT1Areceptor antagonist WAY-100635 on the activity of the neurons in normal and 6-hydroxydopamine（6-OHDA）-lesioned rats.Methods Single unit responses were recorded extracellularly with glass microelectrodes from ventral mPFC neurons in normal rats and 6-OHDA unilaterally lesiond rats in vivo.Results 6-OHDA lesion of the substantia nigra pars compacta（SNc）significantly increased the firing rate with no change in the firing pattern of neurons of the ventral mPFC in rats.Systemic administration of WAY-100635（0.1 mg/kg,i.v.）did not change the mean firing rate and firing pattern of ventral mPFC neurons in normal rats.In contrast,WAY-100635 signifi- cantly decreased the mean firing rate of the neurons in rats with 6-OHDA lesion of the SNc.Conclusion These data suggest that the degeneration of the nigrostriatal pathway results in an increase of neuronal activity of ventral mPFC and dysfunction of 5-HT1Areceptor.展开更多
文摘目的:5-HT1A基因C-1019G多态性与多种神经精神疾病如抑郁症、精神分裂症、焦虑症等的发生风险、症状严重程度及治疗效果存在关联。本研究旨在建立一种直接巢式PCR结合测序检测该位点的方法。方法:以口腔上皮细胞粗处理物为材料,通过直接巢式PCR扩增包含5-HT1A基因C-1019G位点的靶片段,PCR产物经桑格测序鉴定基因型。结果:所检样本均能扩增出预期大小的PCR产物,测序峰图清晰。结论:成功建立了一种直接巢式PCR结合测序鉴定5-HT1A基因型的方法,有良好的应用前景。Objective: The C-1019G polymorphism of the 5-HT1A gene has been associated with the risk of occurrence, symptom severity, and treatment outcome of a variety of neuropsychiatric disorders, such as depression, schizophrenia, and anxiety disorders. The aim of this study was to establish a direct nested PCR combined with sequencing to detect this locus. Methods: The target fragment containing the C-1019G locus of the 5-HT1A gene was amplified by direct nested PCR using crude processed oral epithelial cells, and the PCR product was genotyped by Sanger sequencing. Results: PCR products of expected size were amplified from all the samples tested, and the sequencing peaks were clear. Conclusion: A direct nested PCR combined with sequencing method was successfully established to identify the genotypes of 5-HT1A gene, which has good prospects for application.
文摘目的:通过观察地黄饮子对PSD大鼠5-羟色胺1A受体(5-HT1AR)与5-羟色胺2A受体(5-HT2 AR)mRNA水平表达的影响,进一步探讨地黄饮子治疗脑卒中后抑郁的可能作用机理。方法:在脑卒中后抑郁大鼠模型上,采用实时荧光定量RT-PCR方法,对PSD大鼠5-HT1AR与5-HT2 AR mRNA水平进行观察。结果:地黄饮子高、中剂量和百优解均能明显提高PSD大鼠海马5-HT1AR mRNA表达水平、降低5-HT2 AR mRNA表达水平(P<0.05);地黄饮子高、中剂量与百优解无显著差异(P>0.05);地黄饮子低剂量作用不明显。结论:地黄饮子可能是通过上调5-HT1AR mRNA、下调5-HT2 ARmRNA在海马区的表达,达到治疗PSD的目的。
基金the National Natural Science Foundation of China（No.30370464） ；the Science and Technological Program of Shaanxi Province,China（No.2005K13-G6）
文摘Objective The ventral part of the medial prefrontal cortex（mPFC）plays an important role in initiation and control of voluntary movement,mood and cognition.However,after the degeneration of the nigrostriatal pathway,the neuronal activity of the ventral mPFC and the role of serotonin1A（5-hydroxytryptamine,5-HT1A）receptors in the firing of the neurons are still unknown.The present study is to investigate the change of neuronal activity in the ventral mPFC and the effect of systemic administration of the selective 5-HT1Areceptor antagonist WAY-100635 on the activity of the neurons in normal and 6-hydroxydopamine（6-OHDA）-lesioned rats.Methods Single unit responses were recorded extracellularly with glass microelectrodes from ventral mPFC neurons in normal rats and 6-OHDA unilaterally lesiond rats in vivo.Results 6-OHDA lesion of the substantia nigra pars compacta（SNc）significantly increased the firing rate with no change in the firing pattern of neurons of the ventral mPFC in rats.Systemic administration of WAY-100635（0.1 mg/kg,i.v.）did not change the mean firing rate and firing pattern of ventral mPFC neurons in normal rats.In contrast,WAY-100635 signifi- cantly decreased the mean firing rate of the neurons in rats with 6-OHDA lesion of the SNc.Conclusion These data suggest that the degeneration of the nigrostriatal pathway results in an increase of neuronal activity of ventral mPFC and dysfunction of 5-HT1Areceptor.
