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5-Bromo-2'-deoxyuridine labeling:historical perspectives,factors infiuencing the detection,toxicity,and its implications in the neurogenesis
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作者 Joaquín Martí-Clúa 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第2期302-308,共7页
The halopyrimidine 5-bromo-2′-deoxyuridine(BrdU)is an exogenous marker of DNA synthesis.Since the introduction of monoclonal antibodies against BrdU,an increasing number of methodologies have been used for the immuno... The halopyrimidine 5-bromo-2′-deoxyuridine(BrdU)is an exogenous marker of DNA synthesis.Since the introduction of monoclonal antibodies against BrdU,an increasing number of methodologies have been used for the immunodetection of this synthesized bromine-tagged base analogue into replicating DNA.BrdU labeling is widely used for identifying neuron precursors and following their fate during the embryonic,perinatal,and adult neurogenesis in a variety of vertebrate species including birds,reptiles,and mammals.Due to BrdU toxicity,its incorporation into replicating DNA presents adverse consequences on the generation,survival,and settled patterns of cells.This may lead to false results and misinterpretation in the identification of proliferative neuroblasts.In this review,I will indicate the detrimental effects of this nucleoside during the development of the central nervous system,as well as the reliability of BrdU labeling to detect proliferating neuroblasts.Moreover,it will show factors influencing BrdU immunodetection and the contribution of this nucleoside to the study of prenatal,perinatal,and adult neurogenesis.Human adult neurogenesis will also be discussed.It is my hope that this review serves as a reference for those researchers who focused on detecting cells that are in the synthetic phase of the cell cycle. 展开更多
关键词 5-bromo-2′-deoxyuridine adult neurogenesis human adult neurogenesis LABELING pitfalls prenatal neurogenesis proliferation S-PHASE suturing S-phase TOXICITY
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Kinetic, electronic properties and conductivity studies of ((12Z)N-(5Z)-1-bromo-5-(phenylimino)-1H-pyrrol-2(5H)-ylidene benzenmine {ArN(CNBrCCHCH)NAr} (Ar = C6H5) 被引量:1
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作者 Salem Et. Ashoor Noori Y. Salman 《Journal of Chemistry and Chemical Engineering》 2009年第10期51-56,共6页
Kinetic and conductivity of new Schiff bases compounds such ((12Z)N-(5Z)-1-bromo-5-(phenylimino)-1H- pyrrol-2(5 H)-ylidene)benzenamine {ArN(CNBrCCHCH)NAr} (Ar = C6H5) incorporating the chelating diamino ... Kinetic and conductivity of new Schiff bases compounds such ((12Z)N-(5Z)-1-bromo-5-(phenylimino)-1H- pyrrol-2(5 H)-ylidene)benzenamine {ArN(CNBrCCHCH)NAr} (Ar = C6H5) incorporating the chelating diamino has been studied in this paper and was found a second order of this reaction. Electronic Properties using hyperchem program study has been improved for this compound such angel and bond distance, and then this compound was defining as electric conductivity and did prove to be useful for conduclively compound. 展开更多
关键词 Schiff bases compounds ((12Z)N-(5Z)-1-bromo-5-(phenylimino)- 1H-pyrrol-25H)-ylide ne)benzenamine chelating diamino: electronic properties
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Synthesis,Crystal Structure and Anticancer Property of (Z)-N-(4-Bromo-5-ethoxy-3,5-dimethyl-furan-2(5H)-ylidene)-4-methylbenzenesulfonamide
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作者 张世杰 胡惟孝 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2010年第8期1275-1279,共5页
The novel title compound(Z)-N-(4-bromo-5-ethoxy-3,5-dimethylfuran-2(5H)-yli-dene)-4-methylbenzenesulfonamide 1(C15H18BrNO4S) has been unexpectedly synthesized by the aminohalogenation reaction of ethyl 2-methy... The novel title compound(Z)-N-(4-bromo-5-ethoxy-3,5-dimethylfuran-2(5H)-yli-dene)-4-methylbenzenesulfonamide 1(C15H18BrNO4S) has been unexpectedly synthesized by the aminohalogenation reaction of ethyl 2-methylpenta-2,3-dienoate with TsNBr2,and characterized by mp,IR,1H NMR,EIMS,ESIHRMS and single-crystal X-ray diffraction.It crystallizes in mono-clinic,space group P21/c with a = 11.714(5),b = 14.106(5),c = 10.402(4) ,β = 97.298(8)°,V = 1704.9(12) 3,Mr = 388.27,Z = 4,Dc = 1.513 g/cm3,μ(MoKα) = 2.549 mm-1,F(000) = 792,the final R = 0.033 and wR = 0.062 for 3098 observed reflections(Ⅰ 〉 2σ(Ⅰ)). 展开更多
关键词 synthesis crystal structure N-(4-bromo-5-ethoxy-3 5-dimethylfuran-25H)-ylidene)-4-methylbenzenesulfonamide ANTICANCER
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Theoretical Study on the Reaction Mechanism of 2-Methoxybenzaldehyde,4-Bromo-indanone,Malononitrile and Ammonium Acetate One-pot to Form 6-(2-Methoxyphenyl)-2-amino-6-bromo-5H-indeno[1,2-b]pyridine-3-carbonitrile
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作者 张福兰 黄辉胜 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2017年第10期1685-1696,共12页
The reaction mechanism of 2-methoxybenzaldehyde, 4-bromo-indanone, malononitrile and ammonium acetate one-pot to form 6-(2-methoxyphenyl)-2-amino-6-bromo-5 Hindeno[1,2-b]pyridine-3-carbonitrile was studied by densit... The reaction mechanism of 2-methoxybenzaldehyde, 4-bromo-indanone, malononitrile and ammonium acetate one-pot to form 6-(2-methoxyphenyl)-2-amino-6-bromo-5 Hindeno[1,2-b]pyridine-3-carbonitrile was studied by density functional theory. The geometries of the reactants, transition states, intermediates and products were optimized at the PW91/DNP level. Vibration analysis was carried out to confirm the transition state structure. Reaction pathways were investigated in this study. The result indicates that the reaction Re→ TSB1→IMB1→ TSB2→ IMB2→TSB3→IMB3→TSB4→IMB4→TSB5→IMB5→TSB6→IMB6→TSB7→IMB7→ TSB8→IMB8→TSB9→IMB9→P2 is the main pathway, the activation energy of which is the lowest. The dominant product predicted theoretically is in agreement with the experiment results. 展开更多
关键词 2-methoxybenzaldehyde 4-bromo-indanone 6-(2-methoxyphenyl)-2-amino-6-bromo-5H-indeno[1 2-b]pyridine-3-carbonitrile density functional reaction mechanism
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Synthesis, Crystal Structure and Biological Activities of 3-Bromo-5-(4-chlorophenyl)-4-cyanopyrrole-N,N-dimethyl-2-carboxamide
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作者 许庆博 华云涛 +3 位作者 唐强 周宝晗 陈坤 徐保明 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2018年第5期747-752,共6页
The title compound 3-bromo-5-(4-chlorophenyl)-4-cyanopyrrole-N,N-dimethyl-2-amide(3) was synthesized with 4-bromo-2-(4-chlorophenyl)-5-(trifluoromethyl)-1H-pyrrole-3-carbonitrile(1) and N,N-dimethylformamide... The title compound 3-bromo-5-(4-chlorophenyl)-4-cyanopyrrole-N,N-dimethyl-2-amide(3) was synthesized with 4-bromo-2-(4-chlorophenyl)-5-(trifluoromethyl)-1H-pyrrole-3-carbonitrile(1) and N,N-dimethylformamide(2) by the α-C acylation reaction catalyzed by potassium t-butoxide, and characterized by IR, 1H-NMR and X-ray single-crystal diffraction. It crystallizes in monoclinic, space group P2(1/n)with a = 12.789(2), b = 13.783(2), c = 17.980(3) °, β = 109.230(3)°, V = 2992.5 A3, Mr = 352.62, Dc = 1.565 mg/m3, Z = 8, m = 2.924 mm-1, F(000) = 1408, the final R = 0.0424 and w R = 0.0973 for 3518 observed reflections with I 〉 2σ(I). A total of 23559 reflections were collected, of which 6242 were independent(Rint = 0.0566). The insecticidal, herbicidal and antibacterial activities of compound 3 were determined, and the experimental results showed that the mortality of 3 at the concentration of 100 ppm on the Fipronil against Linnaeus was 76.6%, the growth inhibition rate of 3 against Cynodon Dactylon under the condition of 100 ppm was 35.8% and the inhibitory activity of 3 at the concentration of 25 ppm against Fusarium graminearum reached 50.9%. Hence, the title compound has the value of further research and application prospect. 展开更多
关键词 3-bromo-5-(4-chlorophenyl)-4-cyanopyrrole-N N-dimethyl-2-amide SYNTHESIS crystal structure biological activity
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Synthesis and Crystal Structure of 4-Bromo-5-ethoxy-3-methyl-5- (naphthalen-1-yl)-1-tosyl-1H-pyrrol-2(5H)-one
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作者 沈如伟 杨誉竹 +2 位作者 曹剑 吴露玲 黄宪 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 北大核心 2007年第12期1505-1508,共4页
The title compound 4-bromo-5-ethoxy-3-methyl-5-(naphthalen-l-yl)-l-tosyl-lH- pyrrol-2(5H)-one 1 (C24H22BrNO4S, Mr = 500.40) has been synthesized and its crystal structure was determined by single-crystal X-ray d... The title compound 4-bromo-5-ethoxy-3-methyl-5-(naphthalen-l-yl)-l-tosyl-lH- pyrrol-2(5H)-one 1 (C24H22BrNO4S, Mr = 500.40) has been synthesized and its crystal structure was determined by single-crystal X-ray diffraction analysis. It crystallizes in monoclinic, space group P21/n with a = 8.8562(15), b = 18.118(3), c = 14.055(2)A, β = 99.855(3)^o, V= 2221.9(6)A3, Z = 4, Dc = 1.496 g/cm^3,μ= 1.975 mm^-1, 2 = 0.71073A, F(000) = 1024, R = 0.0607 and wR = 0.1371. 展开更多
关键词 4-bromo-5-ethoxy-3-methyl-5-(naphthalen-1-y1)-1-tosyl-1H-pyrrol-25H)-one synthesis crystal structure
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Synthesis and Crystal Structure of N1-(2-(4-Bromo-2-nitrophenoxy)-2-dimethyl acyloxymethyl)-5-fluorouracil
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作者 贾忠 李靖 +5 位作者 张阳 赵静思 席娜 胡春红 胡敏敏 贺殿 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2016年第7期1047-1053,共7页
The title compound N1-(2-(4-bromo-2-nitrophenoxy)-2-dimethyl acyloxymethyl)-5-fluorouracil(C15H13BrFN3O7, Mr = 446.19) was synthesized and structurally characterized by ^1H-NMR, -(13)C-NMR, ESI-MS and single-c... The title compound N1-(2-(4-bromo-2-nitrophenoxy)-2-dimethyl acyloxymethyl)-5-fluorouracil(C15H13BrFN3O7, Mr = 446.19) was synthesized and structurally characterized by ^1H-NMR, -(13)C-NMR, ESI-MS and single-crystal X-ray diffraction. The compound crystallizes in triclinic, space group P1, with a = 8.3325(6), b = 10.1808(13), c = 11.8194(16) A, α = 73.194(12), β= 72.351(9), γ = 89.509(8)o, V = 911.2(2) A^3, Z = 2, T = 300.79(10) K, μ(Cu Kα) = 3.578 mm^-1, Dc = 1.626 g/cm3, F(000) = 448.0, GOF = 1.060, 5723 reflections measured(8.232≤2θ≤139.592°), 3339 unique(Rint = 0.0184, Rsigma = 0.0254) which were used in all calculations. The final R = 0.0517(I 〉 2s(I)) and wR = 0.1494(all data). Hydrogen bonds and π-π interactions together stabilize the structure of the molecule. The preliminary biological test shows that the title compound has good antitumor activity against A549 in vitro and lower toxicity to normal cells. 展开更多
关键词 crystal structure synthesis N1-(2-(4-bromo-2-nitrophenoxy)-2-dimethyl acyloxymethyl)-5-fluorouracil antitumor activity
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Investigation on influencing factors of 5-HMF content in Schisandra 被引量:5
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作者 XU Qing LI Ying-hua LU Xiu-yang 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2007年第6期439-445,共7页
In order to investigate the influencing factors of 5-hydroxymethyl-2-furaldehyde (5-HMF) content in Schisandra, confirm the theory of 5-HMF deriving mainly from Schisandra processing course, and give some suggestion... In order to investigate the influencing factors of 5-hydroxymethyl-2-furaldehyde (5-HMF) content in Schisandra, confirm the theory of 5-HMF deriving mainly from Schisandra processing course, and give some suggestions about the Schisandra processing method, the 5-HMF contents in decoctions of Schisandra under different heating temperature, decocting time, soaking time, processing methods and treatment with different solvents before decocting the Schisandra were measured by RP-HPLC method. The results showed that there is great difference of 5-HMF level in decoctions from differently processed Schisandra and unprocessed Schisandra; decocting time of 60 rain has some effects on 5-HMF level in decoctions and there is certain quantity 5-HMF in processed Schisandra itself and very little 5-HMF in unprocessed Schisandra. Heating time, heating temperature and treating solvents all have effect on 5-HMF level in decoction of Schisandra. 5-HMF in Schisandra was mainly from processing course. Both long heating time and high heating temperature can increase 5-HMF level in Schisandra. The production of 5-HMF in Schisandra may have some relationships with some polar components, which can dissolve in water, ethanol and acetone, especially in ethanol. To control processing temperature, processing time and treatment with some solvent is very important for controlling 5-HMF level in Schisandra. 展开更多
关键词 Schisandra process Heating temperature Heating time 5-Hydroxymetyl-2-furaldehyde 5-HMF)
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Preconcentration/Separation of Some Metal Ions Using Sodium Dodecyl Sulfate Coated Alumina Modified with Bis(5-bromo- 2-hydroxy-benzaldehyde)-2-methyl-1,5-pentane Diimine (BBHBPDI) Prior to Their Flame-AAS Determination
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作者 Ghaedi, Mehrorang Montazerozohori, Mortez +2 位作者 Marahel, Farzaneh Nejati Biareh, Mehdi Soylak, Mostafa 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2011年第10期2141-2147,共7页
Preconcentration/separation of Co(II), Fe(III), Pb(II), Cr(IIl), Cu(II) and Cd(II) ions using bis(5-bromo- 2-hydroxy-benzaldehyde)-2-methy1-1,5-pentane diimine (BBHBPDI) on SDS coated alumina has been ... Preconcentration/separation of Co(II), Fe(III), Pb(II), Cr(IIl), Cu(II) and Cd(II) ions using bis(5-bromo- 2-hydroxy-benzaldehyde)-2-methy1-1,5-pentane diimine (BBHBPDI) on SDS coated alumina has been reported. The influences of the analytical parameters including pH, ligand and SDS amount, type and concentration of eluent and sample volume on metal ions recoveries were investigated. At optimum values of all variables the relative standard deviation are between 2.5-2.7 and preconcentration factor was 375, while recoveries for all understudy metal ions are higher than 95%, determination limits are between 1.5-2.7. The method has been successfully applied to determination of Co(II), Fe(III), Pb(II), Cr(III), Cu(II) and Cd(II) ions content in some real samples. 展开更多
关键词 surfactant coated alumina atomic absorption spectrometry solid phase extraction bis(5-bromo-2-hydroxy-benzaldehyde)-2-methyl- 1 5-pentane diimine 3 - [(1H-indo1-3y1)- 3 4 5-trimethyl] - 1H-indole
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豚鼠前庭椭圆囊器官在庆大霉素损伤后离体培养的细胞增殖
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作者 孟曦曦 郑少鹏 +2 位作者 刘鋌 王元身 赵春礼 《中华耳科学杂志》 CSCD 2003年第2期1-4,共4页
目的 在离体状态下对豚鼠前庭椭圆囊器官进行培养,观察庆大霉素损伤毛细胞后细胞的增殖情况。方法采用白色健康豚鼠36只,体重300-450克,随机分为4组:(1)正常对照组;(2)正常加BrdU(5-溴基脱氧尿核苷BrdU)组;(3)庆大霉素组;(4)庆大霉素加B... 目的 在离体状态下对豚鼠前庭椭圆囊器官进行培养,观察庆大霉素损伤毛细胞后细胞的增殖情况。方法采用白色健康豚鼠36只,体重300-450克,随机分为4组:(1)正常对照组;(2)正常加BrdU(5-溴基脱氧尿核苷BrdU)组;(3)庆大霉素组;(4)庆大霉素加BrdU组。应用体外组织培养、5—溴基脱氧尿核苷(BrdU,3μg/ml)掺入及免疫组织化学染色和Epon树酯包埋半薄切片等方法,在不同时间(2、3、5、15天)光镜观察庆大霉素(2.0mM)损伤后毛细胞及支持细胞增殖情况。结果 用庆大霉素48小时后,可见到椭圆囊感觉上皮中的毛细胞溶解破坏。加入BrdU继续培养,BrdU免疫组织化学染色显示了第5和15天被BrdU标记的阳性细胞核,阳性细胞核多位于椭圆囊感觉上皮中基底层,从细胞的形态和分布位置上看为支持细胞;感觉上皮的表层,也可观察到BrdU反应阳性的上皮细胞,细胞核较圆,形态和位置类似毛细胞。结论 提示损伤后的椭圆囊感觉上皮支持细胞可以进行细胞的有丝分裂,支持细胞可能是修复毛细胞的前体细胞。 展开更多
关键词 椭圆囊 增殖细胞 体外 庆大霉素 5-溴基脱氧尿核苷(5-bromo-2'-deoxyuridine BrdU)
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Effects of Repetitive Transcranial Magnetic Stimulation on Astrocytes Proliferation and nNOS Expression in Neuropathic Pain Rats 被引量:10
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作者 Lu YANG Sai-hua WANG +3 位作者 Yan HU Yan-fang SUI Tao PENG Tie-cheng GUO 《Current Medical Science》 SCIE CAS 2018年第3期482-490,共9页
This study investigated the effects of different frequencies of repetitive transcranial magnetic stimulation (rTMS) on chronic neuropathic pain in rats. The behavior of rats with experimental chronic neuropathic pai... This study investigated the effects of different frequencies of repetitive transcranial magnetic stimulation (rTMS) on chronic neuropathic pain in rats. The behavior of rats with experimental chronic neuropathic pain was observed, and the expression of neuronal nitric oxide synthase (nNOS) in the ipsilateral dorsal root ganglions (DRGs) and the activation and proliferation of astrocytes in the ipsilateral spinal dorsal horn were detected. Thirty-two male Sprague-Dawley rats were randomly divided into four groups: sham-operated group, sham-rTMS group, 1 Hz group and 20 Hz group (8 rats in each group). Chronic constriction nerve injury induced by sciatic nerve ligation was made to establish the models of the chronic neuropathic pain in rats except those in the sham-operated group. Then we applied different frequencies of rTMS to the primary motor cortex (M1) contralateral to the pain side once daily for 10 consecutive days. Pain behavior scores were observed before and after treatment. Western blot analysis was used to detect the expression of nNOS in ipsilateral L4-6 DRGs. Double immunofluorescent labeling for glial fibrillary acidic protein (GFAP) and 5-bromo-2- deoxyuridine (BrdU) was employed to observe the activation and proliferation of astrocytes in the ipsilateral L4-6 spinal dorsal horn. After rTMS treatment, the spontaneous pain behavior scores were significantly lower in the 20 Hz group than those in the sham-rTMS group (P〈0.05). Moreover, the brush-evoked pain behavior scores were significantly lower in the 20 Hz group than those in the sham-rTMS and 1 Hz group (P〈0.05), suggesting that the spontaneous pain and brush-evoked pain in the 20 Hz group were significantly alleviated. Western blot analysis revealed that the expression of nNOS in ipsilateral L4-6 DRGs was significantly decreased in the 20 Hz group as compared with the sham-rTMS group and the 1 Hz group (P〈0.01) after rTMS treatment. Double immunofluorescence suggested that the expression of GFAP and the co-localization with BrdU in astrocytes were less in the sham-operated group than those in the sham-rTMS group and the 1 Hz group in L4-6 spinal dorsal horn ipsilateral to the neuropathic pain. After rTMS treatment, the expression of GFAP and the co-localization with BrdU decreased in the 20 Hz group as compared with the sham-rTMS group and the 1 Hz group (P〈0.05). In addition, the alleviation degree of spontaneous pain and brush-evoked pain in the 20 Hz group was negatively correlated with the expression of nNOS in ipsilateral DRGs and the number of GFAP/BrdU co-labelled astrocytes in L4-6 spinal dorsal horn ipsilateral to the neuropathic pain (P〈0.05). It was suggested that high-frequency rTMS may relieve neuropathic pain through down-regulating the overexpression of nNOS in ipsilateral DRGs and inhibiting the activity and proliferation of astrocytes in L4-6 spinal dorsal horn ipsilateral to the neuropathic pain. 展开更多
关键词 neuropathic pain repetitive transcranial magnetic stimulation neuronal nitric oxide synthase ASTROCYTE glial fibrillary acidic protein 5-bromo-2-deoxyuridine
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Proliferation and Differentiation of Duct Epithelial Cells after Partial Pancreatectomy in Rats 被引量:3
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作者 刘涛 王春友 +2 位作者 万赤丹 熊炯炘 周峰 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2006年第5期567-569,共3页
The proliferation and differentiation of pancreatic duct epithelial cells in remnant pancreas during regeneration after partial pancreatectomy in rats were studied, and the source of pancreatic stem cells was characte... The proliferation and differentiation of pancreatic duct epithelial cells in remnant pancreas during regeneration after partial pancreatectomy in rats were studied, and the source of pancreatic stem cells was characterized. Partial (90 %) pancreatectomy was performed on 4- to 5-week-old Sprague-Dawley rats, and different duct epithelial cells and acinar cells were detected by immunohistrochemical stain method and scored using 5-bromo-2'-deoxyuridine (BrdU) labeling index (LI) at various time points after partial pancreatectomy. It was found that at 24 h after partial pancreatectomy proliferation started in the main, large and small duct cells, and persisted in small duct cells to day 5. There was significant difference between the experimental group and the control group (P〈0.001). Acinar cells positive for BrdU were greatly increased and reached the peak LI on day 5. The destroyed lobular architecture almost totally recovered on day 7, and the newly islet cells appeared around the pancreatic ducts. These results suggest that regeneration after partial pancreatectomy is involved in proliferation and differentiation of pancreatic stem cells, and pancreatic stem cells may locate in the pancreatic ductules. 展开更多
关键词 PANCREAS stem cells REGENERATION 5-bromo-2'-deoxyuridine RATS
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Nanomolar concentration of alpha-synuclein enhances dopaminergic neuronal survival via Akt pathway 被引量:2
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作者 Ji-Young Kim Beom Seok Jeon +1 位作者 Han-Joon Kim Tae-Beom Ahn 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第35期3269-3274,共6页
Although alpha-synuclein is generally thought to have a pathological role in Parkinson's disease, accumulative evidence exists that alpha-synuclein has a neuroprotective effect. The aim of this study was to evaluate ... Although alpha-synuclein is generally thought to have a pathological role in Parkinson's disease, accumulative evidence exists that alpha-synuclein has a neuroprotective effect. The aim of this study was to evaluate the effect of extracellular alpha-synuclein on dopaminergic cell survival. We assessed cell viability using the 3-(4,5-dimethyt-thiazol-2-yt)-2,5-diphenyltertazolium bromide (MTT) assay both in undifferentiated SH-SY5Y (SHSY) cells and neuronally-differentiated SH-SY5Y (ndSHSY) cells after 24 hour treatment with monomeric alpha-synuclein at various concentrations (0 [control], 50, 100 nmol/L, 1 IJmol/L). To determine whether cell viability assessed by MTT assay was affected by cell proliferation, 5-bromo-2'-deoxyuridine (BrdU) incorporation assay was per- formed. Level of both Akt and phosphorylated Akt was measured using western blot method in ndSHSY cells with or without 24 hour alpha-synuclein treatment. Cell viability was increased in ndSHSY cells at the nanomolar concentration of alpha-synuclein, but not in SHSY cells. Proportion of BrdU-positive ndSHSY cells was decreased in alpha-synuclein-treated group compared with control group. Level of phosphorylated Akt in alpha-synuclein-treated group was higher compared with the control group. Our study shows that extracellular alpha-synuclein at nanomolar concentra- tion benefits dopaminergic cell survival via Akt pathway. 展开更多
关键词 neural regeneration ALPHA-SYNUCLEIN neuronal survival nanomolar extracellular phosphorylatedAkt SH-SY5Y cell neuronal differentiation proliferation DOPAMINERGIC 5-bromo-2'-deoxyuridine grants-supported paper NEUROREGENERATION
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Unexpected BrdU inhibition on astrocyte-to-neuron conversion 被引量:2
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作者 Tao Wang Jian-Cheng Liao +7 位作者 Xu Wang Qing-Song Wang Kai-Ying Wan Yi-Yi Yang Qing He Jia-Xuan Zhang Gong Chen Wen Li 《Neural Regeneration Research》 SCIE CAS CSCD 2022年第7期1526-1534,共9页
5-Bromo-2′-deoxyuridine(BrdU)is a halogenated pyrimidine that can be incorporated into newly synthesized DNA during the S phase of the cell cycle.BrdU is widely used in fate-mapping studies of embryonic and adult neu... 5-Bromo-2′-deoxyuridine(BrdU)is a halogenated pyrimidine that can be incorporated into newly synthesized DNA during the S phase of the cell cycle.BrdU is widely used in fate-mapping studies of embryonic and adult neurogenesis to identify newborn neurons,however side effects on neural stem cells and their progeny have been reported.In vivo astrocyte-to-neuron(AtN)conversion is a new approach for generating newborn neurons by directly converting endogenous astrocytes into neurons.The BrdU-labeling strategy has been used to trace astrocyte-converted neurons,but whether BrdU has any effect on the AtN conversion is unknown.Here,while conducting a NeuroD1-mediated AtN conversion study using BrdU to label dividing reactive astrocytes following ischemic injury,we accidentally discovered that BrdU inhibited AtN conversion.We initially found a gradual reduction in BrdU-labeled astrocytes during NeuroD1-mediated AtN conversion in the mouse cortex.Although most NeuroD1-infected astrocytes were converted into neurons,the number of BrdU-labeled neurons was surprisingly low.To exclude the possibility that this BrdU inhibition was caused by the ischemic injury,we conducted an in vitro AtN conversion study by overexpressing NeuroD1 in cultured cortical astrocytes in the presence or absence of BrdU.Surprisingly,we also found a significantly lower conversion rate and a smaller number of converted neurons in the BrdU-treated group compared with the untreated group.These results revealed an unexpected inhibitory effect of BrdU on AtN conversion,suggesting more caution is needed when using BrdU in AtN conversion studies and in data interpretation. 展开更多
关键词 5-bromo-2′-deoxyuridine NeuroD1 astrocyte-to-neuron conversion reprogramming neural regeneration reactive astrocytes neurons lineage tracing fate mapping neural stem cell
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Synthesis and Nonlinear Optical Property of a Series of New Chromophores Containing Furan Ring as the Only Conjugation Bridge
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作者 Wei ZHANG Jian Li HUA Jin Gui QIN 《Chinese Chemical Letters》 SCIE CAS CSCD 2002年第10期945-948,共4页
This paper reports the synthesis and the nonlinear optical property of a series of new chromophores which contain furan ring as the only conjugation bridge for the first time. They are characterized by UV-VIS, FT-IR, ... This paper reports the synthesis and the nonlinear optical property of a series of new chromophores which contain furan ring as the only conjugation bridge for the first time. They are characterized by UV-VIS, FT-IR, H-1 NMR, MS and elemental analysis. Their dipole moment and the first-order molecular hyperpolarizability (beta) are calculated and compared with those or the analogues containing either benzene or thiophene as file conjugation bridge. 展开更多
关键词 5-diethylamino-2-furaldehyde CHROMOPHORES nonlinear optical materials organic synthesis the first-order molecular hyperpolarizability (b)
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THE DNase-1 SENSITIVE REGIONS IN GENOMES OF BURKITT' S LYMPHOMA CELLS
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作者 李桂源 姚开泰 +2 位作者 潘世成 曹利 刘华英 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1993年第4期14-20,共7页
This article reported the distribution of DNase-1 sensitive regions in genomes of three Burkitt's lymphoma cell lines, P3HR-1, Raji and Ramos cell lines using a new method of in situ nick translation of chromosome... This article reported the distribution of DNase-1 sensitive regions in genomes of three Burkitt's lymphoma cell lines, P3HR-1, Raji and Ramos cell lines using a new method of in situ nick translation of chromosomes substituted completely by BrdU. The results showed that the Blym locus on chromosomes In three cell lines and the c-myc locus on chromosomes in P3HR-1 were the DNase-1 sensitive regions and found that the rearrangemental sites of chromosomes present in three Burkltt' s lymphoma cell lines were sensitive to DNase-1 digestion, Indicating that c-myc, bcl-1 genes located at the rearrangemental sites and the Blym gene in Burkltt' s lymphoma are the active genes having the capability of expression. 展开更多
关键词 5-bromo-2-deoxyuridine DNase-1 Active gene in situ nick translation of chromosome Burkitt's lymphoma CHROMOSOME Chromosomal rearrangement.
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Competition of Aromatic Bromination and Nitration in Concentrated Sulfuric Acid
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作者 Alexander M. Andrievsky Mikhail V. Gorelik 《Open Journal of Synthesis Theory and Applications》 2013年第1期46-50,共5页
Composition of bromine and nitric acid in concentrated sulfuric acid is effective for bromination of strongly deactivated aromatic compounds and for nitration and bromination of moderately deactivated compounds in one... Composition of bromine and nitric acid in concentrated sulfuric acid is effective for bromination of strongly deactivated aromatic compounds and for nitration and bromination of moderately deactivated compounds in one stage. The question why brominating agent is more reactive than nitrating agent in relation to strongly deactivated aromatic compounds and is less reactive in relation to moderate deactivated ones is discussed. 展开更多
关键词 BROMINATION NITRATION AROMATIC Compounds 5-bromo-1 3-dinitrobenzene 5-bromo-2 4 7-trinitrofluorenone 4-bromo-2 7-dinitrophenanthrenquinone
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Synthesis of Folate-Appended <i>β</i>-Cyclodextrin Using Phenanthroline as Linker for Cancer Targeting Drug Delivery
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作者 Nadim S. Russel Punam K. Paul +1 位作者 Mohammad Karim Byeongwoon Song 《International Journal of Organic Chemistry》 2019年第1期47-66,共20页
Here we have synthesized cancer targeting drug delivery system that contains β-cyclodextrin as a drug carrier and folic acid as a targeting ligand. Folic acid was selected as a cancer targeting ligand because folic a... Here we have synthesized cancer targeting drug delivery system that contains β-cyclodextrin as a drug carrier and folic acid as a targeting ligand. Folic acid was selected as a cancer targeting ligand because folic acid receptors are highly expressed in a variety of tumor types. β-cyclodextrin and folic acid were connected through a linker [1,10-phenanthroline or 5-(hydroxymethyl) furfural]. β-cyclodextrin and folic acid were attached to the linker by formation of Schiffbase and ester, respectively. Our targeted synthetic products were confirmed by NMR, Mass and IR spectroscopy. We anticipate that our synthetic products can confer cancer cell-specific drug delivery as well as desired properties such as nanoparticle formation. 展开更多
关键词 Schiff Base β-Cyclodextrin p-Toluenesulfonylchloride Ethylenediamine Folic Acid 2 9-Dimethyl-1 10-PHENANTHROLINE Hemi-Hydrate 5-(Hydroxymethyl-2-furaldehyde)
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Aromatic Bromination in Concentrated Nitric Acid
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作者 Alexander M. Andrievsky Vera I. Lomzakova +1 位作者 Mikhail K. Grachev Mikhail V. Gorelik 《Open Journal of Synthesis Theory and Applications》 2014年第2期15-20,共6页
Action of bromine in concentrated nitric acid allows carrying out mono- and polybromination of moderately deactivated aromatic compounds. 4-Chloronitrobenzene and isophthalic acid turnes into 3-bromo-4-chloronitrobenz... Action of bromine in concentrated nitric acid allows carrying out mono- and polybromination of moderately deactivated aromatic compounds. 4-Chloronitrobenzene and isophthalic acid turnes into 3-bromo-4-chloronitrobenzene and 5-bromoisophthalic acid at reaction with bromine in concentrated nitric acid at 20°C whereas in absence of bromine in the same conditions 4-chloro-1, 3-dinitrobenzene and 5-nitroisophthalic acid are formed accordingly. Presence of bromine in concentrated nitric acid changes nitrating capacity to brominating one. Terephthalic acid and phthalic anhydride at heating with bromine in concentrated nitric acid can be transformed to appropriating tetrabromo substituted compounds. 展开更多
关键词 BROMINATION NITRATION AROMATIC Compounds 3-bromo-4-chloronitrobenzene 5 Bromoisophthalic ACID 2 3 5 6-Tetrabromoterephthalic ACID 4 6 Tetrabromophthalic ANHYDRIDE
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Roles of paroxetine and corticosterone on adult mammalian ciliary body cell proliferation 被引量:2
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作者 WANG Hua LAU Benson WM +6 位作者 YAU Suk-yu LI Suk-yee LEUNG Nelson WANG Ning-li TANG Siu-wa LEE Tatia MC SO Kwok-fai 《Chinese Medical Journal》 SCIE CAS CSCD 2010年第10期1305-1310,共6页
Background The neurogenesis in retina of adult mammals is generally abolished, and this renders the retina lack of regenerative capacity. Despite this, there is a small population of nestin-positive cells in the cilia... Background The neurogenesis in retina of adult mammals is generally abolished, and this renders the retina lack of regenerative capacity. Despite this, there is a small population of nestin-positive cells in the ciliary epithelium which retains neurogenic potential. The present study aimed at investigating the effect of two drugs, corticosterone and paroxetine, on the cell proliferation of the ciliary body. Methods Adult Sprague-Dawley rats were given vehicle, corticosterone, paroxetine, or both corticosterone and paroxetine treatment for 14 days. Cell proliferation in the ciliary body was quantified using 5-bromo-2-deoxyuridine (BrdU) immunohistochemistry. Co-labelling of BrdU and stem cell marker was used to phenotype the BrdU immunoreactive cells. Results Corticosterone treatment suppressed while paroxetine treatment increased the cell proliferation of the ciliary body. Co-labelling with cell markers revealed that the BrdU positive cells also showed nestin expression but not glial fJbrillary acidic protein (GFAP). Conclusions The results illustrate that proliferation of retinal progenitor cells situated in ciliary body are subjected to regulation by selective serotonin reuptake Jnhibitors (SSRI) and corticosteroJd, which is similar to our previous findings in neurogenic regions in central nervous system (CNS). Paroxetine treatment could reverse the suppressive effect of corticosterone on ciliary body cell proliferation. This provides information for future investigation of retinal stem cell biology and potential treatment of retinal degenerative diseases. 展开更多
关键词 PAROXETINE 5-bromo-2-deoxyuridine CORTICOSTERONE ciliary body cell proliferation NEUROGENESIS
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