A new energetic complex [Hg(DAT)Cl2]n was synthesized by the reaction of 1,5-diaminotetrazole with mercury bichloride and characterized by elemental analysis, IR spectroscopy and X-ray single-crystal diffraction. Th...A new energetic complex [Hg(DAT)Cl2]n was synthesized by the reaction of 1,5-diaminotetrazole with mercury bichloride and characterized by elemental analysis, IR spectroscopy and X-ray single-crystal diffraction. The crystal belongs to the monoclinic system with P21/c space group, and a = 4.0342(3), b = 17.7999(12), c = 10.0127(7) , β = 91.558(1)°, V = 718.73(9) 3, Z = 4, CH4Cl2HgN6, Mr = 371.59, Dc = 3.434 g·cm-3, F(000) = 664, S = 1.037, the final R = 0.0223 and wR = 0.0642 for 1646 observed reflections with Ⅰ 〉 2σ(Ⅰ). The central Hg is coordinated by one N atom from 1,5-diaminotetrazole and one mono-dentate terminal chloride and two bi-dentate bridging chloride ligands. Adjacent Hg cations were connected by the bi-dentate Cl to form a 1D zigzag supramolecular chain along the a-axis.展开更多
目的探讨甲基转移酶5(methyltransferase-like 5,METTL5)在三阴乳腺癌(triple-negative breast cancer,TNBC)中的作用和潜在机制。方法采用免疫组织化学方法和Western blot检测TNBC肿瘤组织和细胞系中METTL5的表达情况。用靶向METTL5的s...目的探讨甲基转移酶5(methyltransferase-like 5,METTL5)在三阴乳腺癌(triple-negative breast cancer,TNBC)中的作用和潜在机制。方法采用免疫组织化学方法和Western blot检测TNBC肿瘤组织和细胞系中METTL5的表达情况。用靶向METTL5的shRNA(shRNA-METTL5)转染TNBC细胞后,用CCK-8、集落形成、伤口愈合以及Transwell实验分别检测细胞增殖活性、迁移与侵袭,Western blot检测Wnt/β-catenin信号关键蛋白的表达。构建异种移植瘤模型,验证敲降METTL5对TNBC细胞在体内生长以及Wnt/β-catenin信号活性的影响。结果METTL5在TNBC肿瘤组织和细胞系中表达上调(P<0.01)。敲降METTL5可抑制TNBC细胞的增殖、迁移和侵袭并降低了Wnt/β-catenin信号分子β-catenin、细胞周期蛋白(Cyclin)D1、基质金属蛋白酶(MMP)-2和MMP-7的表达(均P<0.01)。体内实验显示,敲降METTL5减缓了移植瘤生长和Wnt/β-catenin信号活性。结论敲降METTL5能抑制TNBC细胞的增殖、迁移与侵袭,其作用可能与抑制Wnt/β-catenin信号通路有关。展开更多
基金Supported by the National Natural Science Foundation of China (10776002)State Key Laboratory of Science and Technology (No. ZDKT12-03 & QNKT11-06)the Program for New Century Excellent Talents in University (NCET-09-0051)
文摘A new energetic complex [Hg(DAT)Cl2]n was synthesized by the reaction of 1,5-diaminotetrazole with mercury bichloride and characterized by elemental analysis, IR spectroscopy and X-ray single-crystal diffraction. The crystal belongs to the monoclinic system with P21/c space group, and a = 4.0342(3), b = 17.7999(12), c = 10.0127(7) , β = 91.558(1)°, V = 718.73(9) 3, Z = 4, CH4Cl2HgN6, Mr = 371.59, Dc = 3.434 g·cm-3, F(000) = 664, S = 1.037, the final R = 0.0223 and wR = 0.0642 for 1646 observed reflections with Ⅰ 〉 2σ(Ⅰ). The central Hg is coordinated by one N atom from 1,5-diaminotetrazole and one mono-dentate terminal chloride and two bi-dentate bridging chloride ligands. Adjacent Hg cations were connected by the bi-dentate Cl to form a 1D zigzag supramolecular chain along the a-axis.