The internal turbulent flow in conical diffuser is a very complicated adverse pressure gradient flow.DLR k-ε turbulence model was adopted to study it.The every terms of the Laplace operator in DLR k-ε turbulence mod...The internal turbulent flow in conical diffuser is a very complicated adverse pressure gradient flow.DLR k-ε turbulence model was adopted to study it.The every terms of the Laplace operator in DLR k-ε turbulence model and pressure Poisson equation were discretized by upwind difference scheme.A new full implicit difference scheme of 5-point was constructed by using finite volume method and finite difference method.A large sparse matrix with five diagonals was formed and was stored by three arrays of one dimension in a compressed mode.General iterative methods do not work wel1 with large sparse matrix.With algebraic multigrid method(AMG),linear algebraic system of equations was solved and the precision was set at 10-6.The computation results were compared with the experimental results.The results show that the computation results have a good agreement with the experiment data.The precision of computational results and numerical simulation efficiency are greatly improved.展开更多
5-Fluorocytosine (5-FC) is used for the treatment of several infections. It is extremely important to monitor blood level concentration for maximum activity to avoid its side effects. A simple, faster, and more accura...5-Fluorocytosine (5-FC) is used for the treatment of several infections. It is extremely important to monitor blood level concentration for maximum activity to avoid its side effects. A simple, faster, and more accurate analytical method is developed and validated using high-performance liquid chromatography with UV detection in a very low-volume serum sample. Exactly 50 μL of serum was precipitated with 5% trichloroacetic acid. After mixing and centrifugation, 20 μL of supernatant was injected into the HPLC column. Detection was performed at 280 nm. The method is very specific and free from interfering substances due to different drugs and their different circulating metabolites. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.50 μg/L and 1.0 μg/L, respectively. The method was linear in the range of 5 - 150 μg/L in the serum sample. In method comparison, the correlation coefficient r<sup>2</sup> was 0.999 and the percentage recovery was 90% - 105% on four levels of the quality control samples. Within run and between run precision was found to be less than 2.2% at four different concentrations (5, 25, 50, and 100 μg/L). A simple, faster, and more accurate HPLC-UV method is developed which is very useful for monitoring 5-FC concentration in low volume serum samples without evaporation step and ion exchange chromatography within minutes.展开更多
Phosphorus (P) and potassium (K) levels in southern Brazilian (Rio Grande do Sul, RS) soils are largely below the critical concentrations more than four decades after implementation of the officially recommended syste...Phosphorus (P) and potassium (K) levels in southern Brazilian (Rio Grande do Sul, RS) soils are largely below the critical concentrations more than four decades after implementation of the officially recommended system. This study aims to evaluate the increase in P and K levels in 0-10 and 0-20 cm deep samples from no-till soils using the Mehlich-1 (M1) and Mehlich-3 (M3) extractants as well as resin methods and to estimate the amount of P2O5 and K2O fertilizers necessary to increase the P and K soil levels by 1 mg·kg-1. The study was conducted in fields cultivated using a no-till system (direct planting) to grow soybeans, wheat, maize, pasture, and cover crops and fertilized with P2O5 and K2O. Soil samples were collected from the 0-10 and 0-20 cm soil layers and analyzed by the M1, M3, and resin methods. The results demonstrated that the P and K levels increased in the 0-10 and 0-20 cm layers. However, the amount of these levels increased depending on the source of phosphate fertilization and on the P and K extraction methods used. The amount of P2O5 fertilization needed to raise the P level by 1 mg·kg-1 was greater in the 0-20 cm layer than in the 0-10 cm layer, and the amount of K2O fertilization needed to raise the K level by 1 mg·kg-1 was higher in the 0-10 cm layer than in the 0-20 cm layer.展开更多
基金Projects(59375211,10771178,10676031) supported by the National Natural Science Foundation of ChinaProject(07A068) supported by the Key Project of Hunan Education CommissionProject(2005CB321702) supported by the National Key Basic Research Program of China
文摘The internal turbulent flow in conical diffuser is a very complicated adverse pressure gradient flow.DLR k-ε turbulence model was adopted to study it.The every terms of the Laplace operator in DLR k-ε turbulence model and pressure Poisson equation were discretized by upwind difference scheme.A new full implicit difference scheme of 5-point was constructed by using finite volume method and finite difference method.A large sparse matrix with five diagonals was formed and was stored by three arrays of one dimension in a compressed mode.General iterative methods do not work wel1 with large sparse matrix.With algebraic multigrid method(AMG),linear algebraic system of equations was solved and the precision was set at 10-6.The computation results were compared with the experimental results.The results show that the computation results have a good agreement with the experiment data.The precision of computational results and numerical simulation efficiency are greatly improved.
文摘5-Fluorocytosine (5-FC) is used for the treatment of several infections. It is extremely important to monitor blood level concentration for maximum activity to avoid its side effects. A simple, faster, and more accurate analytical method is developed and validated using high-performance liquid chromatography with UV detection in a very low-volume serum sample. Exactly 50 μL of serum was precipitated with 5% trichloroacetic acid. After mixing and centrifugation, 20 μL of supernatant was injected into the HPLC column. Detection was performed at 280 nm. The method is very specific and free from interfering substances due to different drugs and their different circulating metabolites. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.50 μg/L and 1.0 μg/L, respectively. The method was linear in the range of 5 - 150 μg/L in the serum sample. In method comparison, the correlation coefficient r<sup>2</sup> was 0.999 and the percentage recovery was 90% - 105% on four levels of the quality control samples. Within run and between run precision was found to be less than 2.2% at four different concentrations (5, 25, 50, and 100 μg/L). A simple, faster, and more accurate HPLC-UV method is developed which is very useful for monitoring 5-FC concentration in low volume serum samples without evaporation step and ion exchange chromatography within minutes.
基金Authors are thankfully to all Universities and agricultural extensions programs from Rio Grande do Sul State that kindly support this work allowing access in their experiments to collect informationto support us with crop yield and crop biomass data.
文摘Phosphorus (P) and potassium (K) levels in southern Brazilian (Rio Grande do Sul, RS) soils are largely below the critical concentrations more than four decades after implementation of the officially recommended system. This study aims to evaluate the increase in P and K levels in 0-10 and 0-20 cm deep samples from no-till soils using the Mehlich-1 (M1) and Mehlich-3 (M3) extractants as well as resin methods and to estimate the amount of P2O5 and K2O fertilizers necessary to increase the P and K soil levels by 1 mg·kg-1. The study was conducted in fields cultivated using a no-till system (direct planting) to grow soybeans, wheat, maize, pasture, and cover crops and fertilized with P2O5 and K2O. Soil samples were collected from the 0-10 and 0-20 cm soil layers and analyzed by the M1, M3, and resin methods. The results demonstrated that the P and K levels increased in the 0-10 and 0-20 cm layers. However, the amount of these levels increased depending on the source of phosphate fertilization and on the P and K extraction methods used. The amount of P2O5 fertilization needed to raise the P level by 1 mg·kg-1 was greater in the 0-20 cm layer than in the 0-10 cm layer, and the amount of K2O fertilization needed to raise the K level by 1 mg·kg-1 was higher in the 0-10 cm layer than in the 0-20 cm layer.
