酵母的核糖体5.8 S rDNA及两侧的转录间隔区(ITS)具有显著的种间差异性,可以作为鉴别酵母菌种的分类依据.通过菌落PCR扩增该区域的DNA片段,用3种限制性内切酶CfoⅠ,HaeⅢ和HinfⅠ进行酶切,电泳分析酶切片段的长度多态性(RFLP),然后查找...酵母的核糖体5.8 S rDNA及两侧的转录间隔区(ITS)具有显著的种间差异性,可以作为鉴别酵母菌种的分类依据.通过菌落PCR扩增该区域的DNA片段,用3种限制性内切酶CfoⅠ,HaeⅢ和HinfⅠ进行酶切,电泳分析酶切片段的长度多态性(RFLP),然后查找数据库快速准确的鉴定酵母菌种.对本研究室保藏的12株酵母菌株进行分析,确认了已知种类的酿酒酵母(Saccha-romyces cerevisiaeAS 2.516)和路德类酵母(Saccharomycodes ludwigiiAS 2.243)的RFLP与数据库的信息完全一致,并对其余10株未知种类的酵母进行了菌种分类鉴定.展开更多
The 5.8 S ribosomal DNA sequences (5.8S rDNA) and their flanking regions, internal transcribed spacer 1 and spacer 2 (ITS1 and ITS2) of three new isolates in genus Alexandrium (Alexandrium sp. qd1, Alexandrium sp. qd2...The 5.8 S ribosomal DNA sequences (5.8S rDNA) and their flanking regions, internal transcribed spacer 1 and spacer 2 (ITS1 and ITS2) of three new isolates in genus Alexandrium (Alexandrium sp. qd1, Alexandrium sp. qd2, Alexandrium sp. gz) from China were amplified, sequenced, and subjected to phylogenetic analysis. Alexandrium sp. gz and Alexandrium sp. qd1 were grouped with high bootstrap values with four strains/species, i.e., A. catenella South Korea strain, A. catenella Japan strain, and two from China, Alexandrium sp. AC03 and Alexandrium sp. AN01 being proposed to be A. catenalla in a previous study. Then Alexandrium sp. gz and Alexandrium sp. qd1 were identified as Alexandrium catenella. As A. catenella was isolated from Qingdao and Guangzhou sea areas, it supposedly distributed at least in these two areas and was genetically different. Alexandrium sp. qd2 differed greatly from species in Alexandrium. It clustered with Symbiodinium californium, Symbiodinium sp. G15 and Gymnodinium sp. Zhao 01 with 100% bootstrap value; so Alexandrium sp. qd2 affiliates to genus Symbiodinium, and is probably a free-living Symbiodinium species.展开更多
应用构建的5.8S r DNA-ITS基因克隆文库法,分析冬瓜腌制体系不同时期酵母菌的多样性、优势菌种及相关指标的变化。结果表明:冬瓜腌制过程中酵母菌种类丰富,随着腌制时间的不同,卤水中酵母菌组成存在较大差异。腌制早期优势酵母菌为普鲁...应用构建的5.8S r DNA-ITS基因克隆文库法,分析冬瓜腌制体系不同时期酵母菌的多样性、优势菌种及相关指标的变化。结果表明:冬瓜腌制过程中酵母菌种类丰富,随着腌制时间的不同,卤水中酵母菌组成存在较大差异。腌制早期优势酵母菌为普鲁兰类酵母,腌制中、后期,酵母菌群中优势菌种转变为热带假丝酵母。此外,冬瓜腌制过程中酵母菌数量和p H也相应地发生变化。本研究结果为研究酵母菌对浙东传统腌制蔬菜品质的影响提供微生物学基础。展开更多
文摘The 5.8 S ribosomal DNA sequences (5.8S rDNA) and their flanking regions, internal transcribed spacer 1 and spacer 2 (ITS1 and ITS2) of three new isolates in genus Alexandrium (Alexandrium sp. qd1, Alexandrium sp. qd2, Alexandrium sp. gz) from China were amplified, sequenced, and subjected to phylogenetic analysis. Alexandrium sp. gz and Alexandrium sp. qd1 were grouped with high bootstrap values with four strains/species, i.e., A. catenella South Korea strain, A. catenella Japan strain, and two from China, Alexandrium sp. AC03 and Alexandrium sp. AN01 being proposed to be A. catenalla in a previous study. Then Alexandrium sp. gz and Alexandrium sp. qd1 were identified as Alexandrium catenella. As A. catenella was isolated from Qingdao and Guangzhou sea areas, it supposedly distributed at least in these two areas and was genetically different. Alexandrium sp. qd2 differed greatly from species in Alexandrium. It clustered with Symbiodinium californium, Symbiodinium sp. G15 and Gymnodinium sp. Zhao 01 with 100% bootstrap value; so Alexandrium sp. qd2 affiliates to genus Symbiodinium, and is probably a free-living Symbiodinium species.
文摘应用构建的5.8S r DNA-ITS基因克隆文库法,分析冬瓜腌制体系不同时期酵母菌的多样性、优势菌种及相关指标的变化。结果表明:冬瓜腌制过程中酵母菌种类丰富,随着腌制时间的不同,卤水中酵母菌组成存在较大差异。腌制早期优势酵母菌为普鲁兰类酵母,腌制中、后期,酵母菌群中优势菌种转变为热带假丝酵母。此外,冬瓜腌制过程中酵母菌数量和p H也相应地发生变化。本研究结果为研究酵母菌对浙东传统腌制蔬菜品质的影响提供微生物学基础。
