Three intergeneric somatic hybrids (wheat(+) Haynaldia villosa, wheat(+) Bromus inermis, wheat(+) Agropyron elongatum) and one interfamily somatic hybrid between Vitis vinifera and Bupleurum scorzonerifollium were ana...Three intergeneric somatic hybrids (wheat(+) Haynaldia villosa, wheat(+) Bromus inermis, wheat(+) Agropyron elongatum) and one interfamily somatic hybrid between Vitis vinifera and Bupleurum scorzonerifollium were analyzed by PCR with two kinds of primers.The results showed that in the electrophoresis pattern of the PCR products the somatic hybrids had the characteristic bands of two parents and (or) new bands.This research reveals that PCR analysis with 5S rDNA spacer sequence primers can be used for the identification of somatic hybrids at the molecular level and it is a good method because of its simplicity and good reproducibility.展开更多
We collected nine Enteromorpha specimens from the coast of Yantai and evaluated their diversity based on analyses of their ITS(internal transcribed spacer) and 5S rDNA NTS(non-transcribed spacer) sequences.The ITS seq...We collected nine Enteromorpha specimens from the coast of Yantai and evaluated their diversity based on analyses of their ITS(internal transcribed spacer) and 5S rDNA NTS(non-transcribed spacer) sequences.The ITS sequences showed slight nucleotide divergences between Enteromorpha linza and Enteromorpha prolifera.In contrast,multiple highly variable regions were found in the ITS region of Enteromorpha flexuosa.In general,there were more variable sites in the NTS region than in the ITS region in the three species.The variations in 5S rDNA NTS sequences indicated that the molecular diversity of Enteromorpha from the coast of Yantai is very high.However,a phylogenetic tree constructed using 5S rDNA NTS sequence data indicated that genetic differences were not directly related to geographical distribution.展开更多
割手密作为甘蔗育种中重要的野生亲本,具有适应范围广、抗病和抗逆性强的优势。为了确定5S r DNA在不同倍性割手密基因组中的分布位点的数目、区域与拷贝数,本研究应用荧光原位杂交技术在云南82-114(2n=10x=80)和福建89-1-19(2n=13x=104...割手密作为甘蔗育种中重要的野生亲本,具有适应范围广、抗病和抗逆性强的优势。为了确定5S r DNA在不同倍性割手密基因组中的分布位点的数目、区域与拷贝数,本研究应用荧光原位杂交技术在云南82-114(2n=10x=80)和福建89-1-19(2n=13x=104)两份不同倍性割手密材料的间期核和中期染色体上进行5S r DNA定位研究。结果表明:5S r DNA在这两个割手密无性系中的位点数、分布位置以及信号的强弱变化较大。其中,云南82-114割手密有10个信号位点,且基本都在着丝粒附近;福建89-1-19割手密有13个信号位点,部分靠近着丝粒,还有少部分在着丝粒附近的长臂上;同一细胞中不同染色体上的杂交信号有强弱之分,差异明显;而且割手密的5S r DNA位点数与倍性具有相关性,在染色体上没有固定的分布模式,5S r DNA在不同染色体上的拷贝数存在较大的差异。研究结果揭示了割手密染色体结构多样性和5S r DNA拷贝数多样性,可在分子水平上为染色体提供有效的识别标记,有助于物种的进化及亲缘关系的分析。展开更多
文摘Three intergeneric somatic hybrids (wheat(+) Haynaldia villosa, wheat(+) Bromus inermis, wheat(+) Agropyron elongatum) and one interfamily somatic hybrid between Vitis vinifera and Bupleurum scorzonerifollium were analyzed by PCR with two kinds of primers.The results showed that in the electrophoresis pattern of the PCR products the somatic hybrids had the characteristic bands of two parents and (or) new bands.This research reveals that PCR analysis with 5S rDNA spacer sequence primers can be used for the identification of somatic hybrids at the molecular level and it is a good method because of its simplicity and good reproducibility.
基金Supported by the National Key Technology Research and Development Program(No.2008BA49B01)the Key Innovation Program of Chinese Academy of Sciences(No.KZCX2-YW-209)
文摘We collected nine Enteromorpha specimens from the coast of Yantai and evaluated their diversity based on analyses of their ITS(internal transcribed spacer) and 5S rDNA NTS(non-transcribed spacer) sequences.The ITS sequences showed slight nucleotide divergences between Enteromorpha linza and Enteromorpha prolifera.In contrast,multiple highly variable regions were found in the ITS region of Enteromorpha flexuosa.In general,there were more variable sites in the NTS region than in the ITS region in the three species.The variations in 5S rDNA NTS sequences indicated that the molecular diversity of Enteromorpha from the coast of Yantai is very high.However,a phylogenetic tree constructed using 5S rDNA NTS sequence data indicated that genetic differences were not directly related to geographical distribution.
文摘割手密作为甘蔗育种中重要的野生亲本,具有适应范围广、抗病和抗逆性强的优势。为了确定5S r DNA在不同倍性割手密基因组中的分布位点的数目、区域与拷贝数,本研究应用荧光原位杂交技术在云南82-114(2n=10x=80)和福建89-1-19(2n=13x=104)两份不同倍性割手密材料的间期核和中期染色体上进行5S r DNA定位研究。结果表明:5S r DNA在这两个割手密无性系中的位点数、分布位置以及信号的强弱变化较大。其中,云南82-114割手密有10个信号位点,且基本都在着丝粒附近;福建89-1-19割手密有13个信号位点,部分靠近着丝粒,还有少部分在着丝粒附近的长臂上;同一细胞中不同染色体上的杂交信号有强弱之分,差异明显;而且割手密的5S r DNA位点数与倍性具有相关性,在染色体上没有固定的分布模式,5S r DNA在不同染色体上的拷贝数存在较大的差异。研究结果揭示了割手密染色体结构多样性和5S r DNA拷贝数多样性,可在分子水平上为染色体提供有效的识别标记,有助于物种的进化及亲缘关系的分析。
