HPLC法同时测定赶黄草中4种成分

目的建立HPLC法同时测定赶黄草中乔松素-7-O-β-D-葡萄糖苷、pinocembrin-7-O-[4″,6″-(S)-hexahydroxydiphenoyl]-β-D-glucose、thonningianin A、pinocembrin-7-O-[3″-O-galloyl-4″,6″-(S)-hexahydroxydiphenoyl]-β-Dglucose的含量。方法赶黄草80%甲醇提取物的分析采用Agilent ZORBAX SB-C18柱(4.6 mm×250 mm,5μm)流动相乙腈-0.5%甲酸水,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长280 nm。结果4种成分在各自范围内线性关系良好(r≥0.9999),平均加样回收率100.90%~102.04%,RSD 0.82%~2.54%。结论该方法准确稳定,重复性好,可用于赶黄草的质量控制。

Metabolite characterization of Penta-O-galloyl-β-D-glucose in rat biofluids by HPLC-QTOF-MS

Objective： To understand the in vivo metabolic fate of 1,2,3,4,6-Penta-O-galloyl-β-d-glucose（PGG）naturally existed in many medicinal herbs and food plants such as Rhus chinensis,Paeonia suffruticosa,Paeonia lactiflora and Mango.Methods： The metabolites of PGG in rat biofluids were characterized using high performance liquid chromatography combined with quadrupole time-of-flight tandem mass spectrometry（HPLC-QTOF-MS）.Results： Ten metabolites in urine,five metabolites in feces and two metabolites in plasma,were observed when the rats were administrated with a single intravenous injection of PGG（20 mg/kg）.Conclusion： PGG is firstly metabolized to gallic acid,then gallic acid undergoes sulfation,glucuronidation and methylation by rat liver.The determination of metabolites and the proposed metabolic pathway of PGG in vivo will be benefit to gain deeper insights into its pharmacological activities.