目的:^(64)Cu有半衰期较长、化学反应温和的优势,本研究合成新型乏氧分子影像学探针^(64)Cu-ATSM,并与18F-氟硝基咪唑(^(18)F-fluoromisonidazole,18F-FMISO)进行初步比较。方法:使用SIEMENS Eclipse HP 11 Me V小型医用回旋加速器系统,...目的:^(64)Cu有半衰期较长、化学反应温和的优势,本研究合成新型乏氧分子影像学探针^(64)Cu-ATSM,并与18F-氟硝基咪唑(^(18)F-fluoromisonidazole,18F-FMISO)进行初步比较。方法:使用SIEMENS Eclipse HP 11 Me V小型医用回旋加速器系统,Comecer ALCEO EDL、PRF、TADDEO放射性模块和定制铂金靶盘进行64Ni电镀、^(64)Cu纯化和^(64)Cu-ATSM合成。用伽马能谱仪和高效液相色谱(high performance liquid chromatography,HPLC)进行放射核纯度、放化纯度和稳定性检测。在荷人乳腺癌MDB-MA-231模型中进行小动物PET/CT显像并与自制的^(18)F-FMISO进行比较。结果:实现了^(64)Cu-ATSM的自动化生产,产率60%,放射性核纯度>99%,放化纯度>95%,标记物稳定性>6 h。与^(18)F-FMISO一样能反映肿瘤乏氧情况,且注射1 h后T/M值即能达5.28。结论:成功用11 Me V SIEMENS回旋加速器和Comecer ALCEO模块合成了^(64)Cu-ATSM,荷人乳腺癌小动物PET/CT乏氧显像靶/本比高。展开更多
A new production method of no-carrier-added 64Cu was tested using a new target prepared by electroplating of Ni on a silver layer (thickness 35 μm) previously electroplated on a pure copper target support. This metho...A new production method of no-carrier-added 64Cu was tested using a new target prepared by electroplating of Ni on a silver layer (thickness 35 μm) previously electroplated on a pure copper target support. This method meets cost effective production and quality of the produced 64Cu criteria. The quality of the electroplated layers has been tested under the bombardment by more than 200 μA of proton beam using water cooled target system. A separation and purification setup was elaborated to produce high quantity and high specific activity of 64CuCl2 suitable for labeling different ligands in order to be used in therapy and diagnosis. A semi-automated target dissolution and separation system has been developed and achieved for 64Cu production. The separation chemistry is based on a chromatographic column system.展开更多
文摘目的:^(64)Cu有半衰期较长、化学反应温和的优势,本研究合成新型乏氧分子影像学探针^(64)Cu-ATSM,并与18F-氟硝基咪唑(^(18)F-fluoromisonidazole,18F-FMISO)进行初步比较。方法:使用SIEMENS Eclipse HP 11 Me V小型医用回旋加速器系统,Comecer ALCEO EDL、PRF、TADDEO放射性模块和定制铂金靶盘进行64Ni电镀、^(64)Cu纯化和^(64)Cu-ATSM合成。用伽马能谱仪和高效液相色谱(high performance liquid chromatography,HPLC)进行放射核纯度、放化纯度和稳定性检测。在荷人乳腺癌MDB-MA-231模型中进行小动物PET/CT显像并与自制的^(18)F-FMISO进行比较。结果:实现了^(64)Cu-ATSM的自动化生产,产率60%,放射性核纯度>99%,放化纯度>95%,标记物稳定性>6 h。与^(18)F-FMISO一样能反映肿瘤乏氧情况,且注射1 h后T/M值即能达5.28。结论:成功用11 Me V SIEMENS回旋加速器和Comecer ALCEO模块合成了^(64)Cu-ATSM,荷人乳腺癌小动物PET/CT乏氧显像靶/本比高。
文摘A new production method of no-carrier-added 64Cu was tested using a new target prepared by electroplating of Ni on a silver layer (thickness 35 μm) previously electroplated on a pure copper target support. This method meets cost effective production and quality of the produced 64Cu criteria. The quality of the electroplated layers has been tested under the bombardment by more than 200 μA of proton beam using water cooled target system. A separation and purification setup was elaborated to produce high quantity and high specific activity of 64CuCl2 suitable for labeling different ligands in order to be used in therapy and diagnosis. A semi-automated target dissolution and separation system has been developed and achieved for 64Cu production. The separation chemistry is based on a chromatographic column system.
