Correlation analysis of human papillomavirus E6/E7 mRNA detection with diagnosis,prognosis and recurrence risk in patients with cervical epithelioma

BACKGROUND Cervical intraepithelial neoplasia(CIN)is an important precursor of cervical cancer.Early detection and treatment can reduce the incidence of cervical cancer.AIM To investigate the detection rate of human papillomavirus(HPV)E6/E7 mRNA in cervical tissue of patients with different types of epithelial cell neoplasia(CIN)and its relationship with CIN progression and diagnosis.METHODS One hundred women with HPV infection detected by cervical exfoliation cytology between January 2022 and January 2023 were retrospectively selected.These patients were graded CIN based on colposcopy and cervical pathology.The positive expression rates of HPV E6/E7 mRNA and HPV[polymerase chain reaction(PCR)-reverse dot crossing]were compared among all groups.Patients with HPV E6/E7 mRNA expression in the grade 1 CIN group were followed up for 1 yr.The relationship between atypical squamous epithelium and high malignant epithelial neoplasia was investigated by univariate and multivariate analysis.RESULTS The diagnostic sensitivity,specificity,and sensitivity of PCR-reverse point hybrid ization technology for secondary CIN were 70.41%,70.66%,and 0.714,respectively.Sensitivity and specificity for secondary CIN were 752%and 7853%,respectively,the area under the curve value was 0.789.Logistic Multifactorial model analysis revealed that the HPV positive rates and the HPV E6/E7 mRNA positive rates were independent risk factors of CIN grade I(P<0.05).In CIN grade I patients with positive for HPV E6/E7 mRNA,in its orientation to grade CIN patients,in its orientation to grade CIN patients,at 69.2%,compared with patients negative for HPV E6/E7 mRNA(30.8%),significant difference(P<0.05).CONCLUSION HPV E6/E7 mRNA and HPV(PCR-reverse dot hybrid)positive expression have a close relationship with CINgrade disease progression and is an independent risk factor for high-grade CIN lesions.

Interface analysis of 7B52 Al alloy laminated composite fabricated by hot-roll bonding

The bonding interface of 7B52 Al alloy laminated composite （ALC） fabricated by hot rolling was investigated using optical microscopy （OM）, transmission electron microscopy （TEM）, scanning electron microscopy （SEM）, ultrasonic flaw detection （UFD）, and bonding strength tests. The results show that metallurgical bonding is achieved at the interface after composite rolling. The TEM analysis and tensile tests indicate that the 7B52 ALC plate combines high strength of the hard individual layer and good toughness of the soft individual layer. However, UFD technology and SEM analysis prove that the defects （thick oxide films, acid washed residues, air, oil and coarse particles） existing in the bonding interface are harmful to the bonding strength. To sum up, the composite roiling process is suitable for 7B52 ALC plate, and the content and size of the defects should be controlled strictly. Advanced surface treatment of each individual layer would be beneficial to further improve the bonding quality.

2021-2022年河南省猪细小病毒1~7型检测与分析

旨在了解猪细小病毒1~7型(PPV1~PPV7)在河南省的流行情况。利用PCR检测了2021-2022年河南省猪场送检的748份样品,并对检测数据进行了分析。结果表明,PPV1/2/3/5/6/7在河南省各地区一年四季广泛流行,而秋、冬季节是感染高峰期,PPV2(16.04%)的感染率最高,PPV7(15.11%)次之。产房仔猪未检出PPV1/3/5,PPV2/6/7在各生长阶段猪群中均有检出,患呼吸系统疾病的保育和育肥猪中PPV各型检出率均较高,尤以PPV2常见。PPV2和PPV7在口腔液、血样、肺脾淋巴结中检出率均较高,血样中较低,PPV3常存在于血清和组织中,PPV5多见于口腔液,PPV6多见于血样。研究证实,除PPV4未检出外,其他6种PPV在河南均存在,并且多与其他病原混合感染,PPV2、PPV7可与多种病原混合感染,PPV7与PCV2混合感染最常见。

Analysis on Resistance of Rice Cultivar Lianjing 7 to Rice Black-streaked Dwarf Disease

To analyze the resistance of Lianjing 7 against rice black-streaked dwarf disease and find the molecular markers closely linked with the resistance gene, an F2：3 population derived from an RBSDD-resistant cultivar Lianjing 7 and an RBSDD-susceptible cultivar Peiai 64, was evaluated using natural infection methods and artificial inoculation by scoring the disease severity rating. The results showed Lianjing 7 was resistant to RBSDD, with the incidence between 5.5% and 12.25% in all tests. For the parents of Lianjing 7, only Zhongjingchuan-2 was resistant to RBSDD. Besides this, Lianjing 7 assumed an antixenosis and antibiosis score similar with susceptible variety Wuyujing 3, which indicates that so RBSDD-resistance of Lianjing 7 is mainly caused by the resistance specifically to RBSDV. In the F2：3 population of Lianjing 7/Peiai 64, the disease severity rates showed a continuous distribution with transgressive segregation, it indicated the resistance of Lianjing 7 was controlled by multi-genes. The marker RM287 was determined closely linked with the resistance locus via BSA method. To conclude, Lianjing 7 has a high resistance to RBSDD, which is conferred with by Zhongjingchuan-2, and the marker RM287 should be useful for breeding novel RBSDD-resistant cultivars via marker-assisted selection （MAS）.

A Calculation of Steady Pressure Drop and an Analysis of HT-7U CICC

Under the condition of steady state, the pressure drop of coolant is mainly caused by friction along the cable. In the CICC (cable-in-conduit-conductor), helium flow within the conductor consists of two parallel interconnected tubes. The velocity distribution has some differece between the central channel and conductor space. The region of Reynolds number is from 103 to 106. This paper describes the calculation of pressure drop of HT-7U CICC at various mass flows. It is assumed that the coolant flows in two parallel, rough tubes during the calculation.

Sequencing analysis of matrix metalloproteinase 7-induced genetic changes in Schwann cells

Previous research revealed the positive activity of matrix metalloproteinase 7(MMP7) on migration and myelin regeneration of Schwa nn cells(SCs). However, understanding of the molecular changes and biological activities induced by increased amounts of MMP7 in SCs remains limited. To better understand the underlying molecular events, primary SCs were isolated from the sciatic nerve stump of newborn rats and cultured with 10 nM human MMP7 for 24 hours. The results of genetic testing were analyzed at a relatively relaxed threshold value(fold change ≥ 1.5 and P-value < 0.05). Upon MMP7 exposure, 149 genes were found to be upregulated in SCs, whereas 133 genes were downregulated. Gene Ontology analysis suggested that many differentially expressed molecules were related to cellular processes, single-organism processes, and metabolic processes. Kyoto Enrichment of Genes and Genomes pathway analysis further indicated the critical involvement of cell signaling and metabolism in MMP7-induced molecular regulation of SCs. Results of Ingenuity Pathway Analysis(IPA) also revealed that MMP7 regulates biological processes, molecular functions, cellular components, diseases and functions, biosynthesis, material metabolism, cell movement, and axon guidance. The outcomes of further analysis will deepen our comprehension of MMP7-induced biological changes in SCs. This study was approved by the Laboratory Animal Ethics Committee of Nantong University, China(approval No. 20190225-004) on February 27, 2019.

Cloning and Characterization of Porcine TSARG7 Gene and Analysis of Its Tissue-Specific Expression

TSARG7 is a novel member of the acyltransferase family since its sequence possesses the highly conserved phosphate acyltransferase （PlsC） domain existing in all acyltransferase-like proteins. The porcine TSARG7 had been identified by cloning in silico but had not been confirmed experimentally. The full-length mRNA of porcine TSARG7 gene was sequenced and two splice variants were discovered. The full-length cDNA of TSARG7 variant 1 was 2 513 bp and variant 2 was 2 634 bp. The putative porcine TSARG7 proteins, which were located in the cytoplasm, encoded 458 and 456 amino acids, respectively. Real-time PCR analysis showed that TSARG7 gene was expressed in various tissues, but at different levels. The expression levels of this gene were higher in the skeletal muscle, heart, and testis than that in other tissues, suggesting that the TSARG7 gene played a role in procine skeletal muscle, heart, and testis functions.

基于时空变源分布式模型的流域洪水复盘分析方法——以海河流域北三河系“23·7”较大洪水为例

海河流域北三河系覆盖北京、天津、河北三省(直辖市),其防洪安全事关当地社会经济发展。为有效应对气候变化影响下极端暴雨频发所带来的洪水防御新挑战,提出了一种时空双维度流域性洪水复盘新思路,以“23·7”北三河系较大洪水为例,通过构建时空变源分布式水文模型结合暴雨时空统计分析对全河系洪水的发生、发展过程进行了模拟。模拟结果的确定性系数均大于0.7,洪峰相对误差均小于5%,所建模型可以较好地表征此次洪水的产流机制变化。分析发现,此次洪水过程中北运河流域产流以蓄满产流机制为主,潮白河流域产流则蓄满及混合产流两种机制均有体现,特别是靠近降雨集中区的山区平原过渡地带表现出明显的混合产流机制。建议在未来进一步完善流域防洪体系,重点提升该区域的水雨情监测能力。所提出的时空双维度下的流域性洪水复盘分析方法可为今后其他流域级洪水模拟复盘提供技术参考。

The Analysis and Calculation for the Toroidal Magnetic Field of HT-7U

The HT-7U super-conducting tokamak is a full super-conducting magnetically confined fusion device, It mainly consists of super-conducting toroidal field (TF) coils and super conducting poloidal field (PF) coils. This paper describes the distribution of magnetic field, ripple and electromagnetic loads of TF system, some results are necessary to analyze and calculate the stresses and deformation on TF system by a finite element method. Meanwhile, in this paper, the main scope of the calculation is carried out for the case of constant magnetic field on conductor of the TF coil winding in order to provide electromagnet parameters for the quench analysis of Cable-in-Conduit Conductor (CICC) of TF system in HT-7U.

Linear Buckling Analysis of the HT-7U Vacuum Vessel

The vacuum vessel of the HT-7U superconducting Tokamak is designed as an allmetal welded double-wall structure with a number of radial and vertical ports. With characteristicsof ultrahigh vacuum and thin shell, the analysis on stability is very important to the design. Toachieve a successful final design, a threedimension buckling model has been performed using thefinite element program CoSMOS/M2.0. For all the cases having been considered, a 1/16 segmentof the whole toric shell are used to calculate the linear critical buckling load (Pc.,,) under auniform and nonwhform external pressure. As expected, the structure has a good capability ofwithstanding the applied loads.

肾透明细胞癌中双硫死亡核心基因SLC7A11的孟德尔随机化及生物信息学分析

目的分析溶质载体家族7成员11(SLC7A11)在肾透明细胞癌(ccRCC)发生、发展中的作用及其预后价值。方法采用两样本孟德尔随机化分析以识别与ccRCC风险存在因果关系的基因。使用来自UCSC Xena泛癌队列的RNA测序数据及临床数据分析SLC7A11的表达及预后意义。使用TCGA-KIRC数据(训练集)进行基因集富集分析(GSEA)。随后通过逐步Cox回归分析建立了基于SLC7A11的预后模型,并在E-MATB-1980队列(验证集)中进行了外部验证。结果孟德尔随机化分析显示,SLC7A11水平升高会加重ccRCC的患病风险(HR=1.27,95%CI:1.15~1.40,P<0.001)。SLC7A11在各种肿瘤中过表达,并与高T分期和较差的生存预后相关(P<0.05)。GSEA显示SLC7A11富集在增殖和转移相关通路,包括E2F和上皮-间质转化信号通路。SLC7A11预后模型在训练集(1、3、5年AUC=0.78、0.73、0.71)和验证集(1、3、5年AUC=0.70、0.71、0.72)中均显示出强大的预测性能。结论SLC7A11作为ccRCC的潜在生物标志物和治疗靶点,为精准医学提供了新视角。

一种针对Virtex-7加密位流的侧信道分析方法

随着FPGA在商业、国防等领域的广泛应用,出现了很多针对FPGA的攻击方法,电路安全性面临着极大挑战。为了进一步研究FPGA的安全机制,文章介绍了一种新的侧信道分析(SCA)方法,并首次在Xilinx Virtex-7芯片上分析了加密位流在加载过程中存在的安全漏洞。相比之前的攻击目标,Virtex-7芯片规模更大,采集的信号信噪比更低,攻击难度更大。之前的研究使用的是SASEBO或SAKURA这类专为SCA设计的测试板,而该文的分析是第一个在Xilinx官方评估板上进行的实例,由于官方评估板不是针对侧信道信号采集设计的电路板,因此需要经过处理才能获得足够的信噪比。使用电磁辐射作为侧信道测量值,在80万条电磁曲线内就能够获得一组密钥。通过密钥解密,得到明文位流,攻击者就能够对FPGA进行逆向分析、克隆等操作,从而影响FPGA的安全。

北京地区“23•7”特大暴雨型地质灾害特征及预警成效分析

极端降雨常伴随群发性地质灾害发生,严重危害易发区人民群众生命财产安全,影响经济社会健康发展。总结分析极端降水灾害时空分布特点及预警成效,对于提高地质灾害综合防御能力具有重要意义。以2023年“23•7”强降水引发的突发地质灾害为研究对象,以“北京市突发地质灾害监测预警系统”精细化降水数据为基础,分析了“23•7”强降水时空分布特性和地质灾害发育分布特征,剖析了地质灾害预警成效,结果表明:“23•7”强降水具有总量大、雨强大、历时长、范围广等特点,极端降水灾害具有群发性,地质灾害分级分类多维度预警成效显著,实现了极端天气条件下因地质灾害零伤亡的目标。研究成果可为积极防范和科学应对极端降水地质灾害提供参考。

SNHG7对结肠癌预后影响的生物信息学分析

目的研究长链非编码RNA(lncRNA)核仁小RNA宿主基因7(SNHG7)的表达程度与结肠癌预后的关系,以期寻找一种新型潜在的结肠癌标记物及治疗新靶点。方法从癌症基因组图谱(TCGA)数据库下载所有结肠癌转录数据,提取其中表达SNHG7的数据并整理成矩阵资料,利用R软件对数据进行分析,通过SNHG7差异性表达,生存分析、受试者工作特征曲线(ROC曲线),单因素多因素独立预后分析及结合临床数据特征探讨SNHG7与结肠癌患者预后的相互联系。结果SNHG7在结肠癌标本中高表达,SNHG7高表达组与SNHG7低表达组相比总体生存率明显降低,单因素及多因素回归分析显示SNHG7可作为结肠癌患者预后不佳的独立危险因素(P<0.05)。临床数据相关性研究提示SNHG7的表达量与SNHG7甲基化程度相关,SNHG7甲基化程度与结肠癌的N分期、种族相关(P<0.05)。结论SNHG7为结肠癌患者预后差的危险因素,可以作为结肠癌患者诊断和预后的潜在标志物,并为治疗结肠癌靶点提供新方向。

Clinical manifestations and the prenatal diagnosis of trisomy 7 mosaicism:Two case reports

BACKGROUND The clinical manifestations of trisomy 7 mosaicism are diverse and nonspecific,so prenatal diagnosis is very difficult.CASE SUMMARY Two pregnant women with abnormal prenatal screening results were included.One was a 22-year-old woman(G1P0).At 31st week of gestation,ultrasound revealed that the posterior horn of the left lateral ventricle was 10 mm and the right renal pelvis had a separation of 7 mm.The other pregnant woman was 33 years old(G2P1L1A0),and her fetus was found to have a cardiac malformation at the 24th week of gestation.Copy number variation sequencing,whole-exome sequencing and karyotype analysis were carried out after amniocentesis,and both fetuses were diagnosed with trisomy 7 mosaicism.After parental counseling,one woman continued the pregnancy,and the other woman terminated the pregnancy.CONCLUSION In trisomy 7 mosaicism,the low proportion of trisomy does not lead to abortion,but can result in abnormal fetal development,which can be detected via ultrasound.Therefore,clinicians need to pay more attention to various aspects of fetal growth and development,combining with imaging,cellular,molecular genetics and other methods to perform comprehensive evaluations of fetuses to provide more reliable genetic counseling for pregnant women.

Cloning and Sequence Analysis of IGFBP-7 Gene in Sheep

In this study, full-length CDS sequence of IGFBP-7 gene was cloned from Liangshan semi-fine wool sheep with RT-PCR method and analyzed with bioinformatics methods. The results showed that the full-length CDS sequence of IGFBP-7 gene in Liangshan semi-fine wool sheep was 846 bp in length, encoding 282 amino acids. The CDS sequence shared 99%, 95% and 90% homology with bovine, human and rat, respectively; the amino acid sequence shared 98%, 93% and 89%, respectively. The GenBank accession number was FJ589640.1. The amino acid molecular weight of IGFBP-7 was 29.0 kD, and the theoretical isoelec- tric point （pl） was 8.25. The result of phylogenetic analysis showed that IGFBP-7 gone in Liangshan semi-fine wool sheep exhibited close phylogenetic relationships with bovine, goat and other mammals, and distant phylogenetic relationships with Danio rerio and Haliotis diversicolor. IGFBP-7 gene had uniformly distributed hy- drophobic and hydrophilic regions, harboring one signal peptide, two transmembrane regions, 16 phosphorylation sites, four N-glycosylation sites and one O-glyco- sylation site. The result of secondary structure analysis showed that the random coil, or-helix and β-sheet regions accounted for 64.89%, 19.86% and 15.25%, respectively. The result of tertiary structure analysis showed that IGFBP-7 harbors an IGFBP_N domain and an Ig-like domain. This study provided scientific basis for further investigating the function of IGFBP-7 gene in sheep.

郑州“7·20”特大暴雨移置合肥市洪涝分析

分析郑州“7·20”特大暴雨形成条件,合肥市发生郑州“7·20”特大暴雨的可能性是存在的。为深刻吸取特大暴雨灾害教训,将郑州“7·20”特大暴雨移置至合肥市,开展模拟计算和洪涝分析,为防范此类暴雨洪水、完善防御超标准洪水预案及城市防洪规划提供参考。

KINEMATIC ANALYSIS OF THE GENERAL SPATIAL 7R MECHANISM

The displacement, velocity and acceleration analysis of the general spatial 7R mechanism is discussed in this paper, fused on the method proposed in Ref. [2], an input-output algebra equation of the 16th degree in the tan-half-angle of the output angular displacement is derived. The derivation process and computation are considerably simple. A program written in Allanguage is used to derive the coefficients of displacement equations: therefore the amount of manual work is greatly decreased. The results are verified by a numerical example. The researches of this paper and Ref. [5]found a base for establishing an expert system of spatial mechanism analysis in the future.

The Analysis of Human Papillomavirus Type 16 E6/E7 Genetic Variability in Jingjiang, Jiangsu Province, China

Human papillomavirus 16 (HPV-16) is a major high-risk type causing cervical cancer. The E6 and E7 genes in HPV-16 are the major virulent genes in HPV, and we wanted to investigate the polymorphism of E6 and E7 genes of HPV-16 originated from Jingjiang, Jiangsu province. In research, HPV-16 sample was collected, and the E6 and E7 genes fragments were amplified by PCR assay. The sequences of E6 and E7 genes were used for phylogenetic analysis by Mega 5.0 software. By comparison, the major mutations of E6 gene were T178G (41.67%) and G658A (17%), as well as C58G, T61A and G188C (14%), T61C and C656A (11%). The mutation of E7 gene was mainly C491A and T935A (23%);G514C, G937C and G519C (11%). The 68 nucleotide site deficiency (69.4%) of E6 and 535 nucleotide site deficiency (23%) of E7 were dominant. In our study, we did not find the relevance between the E6 and E7 genes mutations and pathologic severity. But we think the E6 and E7 genes mutation of HPV-16 might affect vaccine prevention and treatment effect.

Structure analysis and function prediction of human P2X7R based on bioinformatics

Objective:To analyze the structural features of human P2X7R protein,meanwhile homology,protein-protein interaction,and cell epitope were predicted to provide theoretical basis for the diagnosis and treatment of related diseases.Methods:Human P2X7R protein was analyzed and predicted by ProtParam、TMHMM、ProtScale and SignalP 5.0 Server et al.Results:Human P2X7R protein is composed of 595 amino acids,the molecular formula is C 3077 H 4749 N 835 O 871 S 40,with the theoretical isoelectric point PI of 8.62,the instability coefficient is 46.07,the total average hydrophilicity is-0.317,and the protein is an hydrophilic protein instead of secreted protein,no any signal peptide,but transmembrane region is contained,which belong to transmembrane protein.There are 157αhelices and 288 random coils in the secondary structure of human P2X7R protein,accounting for 26.39%and 48.40%of all amino acids,The coverage rate of the three-level structure prediction results is 100%,and the sequence consistency is 80.34%,and the ramachandran plot show that the predicted structure is relatively stability.The interaction of proteins show that there are a variety of proteins interacting with human P2X7R,and P2X7R was closely related to inflammation;this protein had many B and T cell epitopes,which could provide ideas for vaccine development.Conclusion:The results indicate that the analysis of human P2X7R protein that is based on bioinformatics method has certain reliability.P2X7R is involved in the development of inflammatory diseases,and the antigenic epitope of B,Th and CTL cells screened out by prediction will provide the treatment scheme for diseases.