选取传统中药提取单体化合物α-倒捻子素和8-甲氧基补骨脂素,通过2种药物敏感性实验方法MABA(Microdilution Alamar Blue Assay)分析与试管法分别测定了两种化合物对结核分枝杆菌H37Rv(ATCC27294)和H37Ra(ATCC25177)的体外抗菌活性,同...选取传统中药提取单体化合物α-倒捻子素和8-甲氧基补骨脂素,通过2种药物敏感性实验方法MABA(Microdilution Alamar Blue Assay)分析与试管法分别测定了两种化合物对结核分枝杆菌H37Rv(ATCC27294)和H37Ra(ATCC25177)的体外抗菌活性,同时还测定了9种阳性抗结核药物对这两种结核菌的最低抑菌浓度(MIC),比较2种方法的检测结果。结果表明:α-倒捻子素对结核分枝杆菌H37Rv(ATCC27294)和H37Ra(ATCC25177)的最低抑菌浓度均为6.25μg/mL,而8-甲氧基补骨脂素对结核分枝杆菌H37Rv(ATCC27294)和H37Ra(ATCC25177)的最低抑菌浓度均为128μg/mL。Alamar B1ue法与试管法相比较,完全符合率是90%(9/11),具有较好的一致性。本研究结果表明Mamar B1ue法是一种快速、简便测定药物对结核分枝杆菌MIC的方法。本研究为α-倒捻子素和8-甲氧基补骨脂素的进一步开发利用和抗分枝杆菌机制研究打下了基础。展开更多
AIM To study the effects of psoralen (PSO) and 8 methoxypsoralen (8 MOP) on the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells. METHODS Inhibitory effects of PSO and 8 MOP on the prolifer...AIM To study the effects of psoralen (PSO) and 8 methoxypsoralen (8 MOP) on the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells. METHODS Inhibitory effects of PSO and 8 MOP on the proliferation and metastatic potential of mucoepidermaid carcinoma Mc3 cells were investigated with MTT assay, cell counting, flow cytometry and tail vein injection of the cells into nude mice (10 6 for each). RESULTS PSO and 8 MOP inhibited Mc3 cell growth in a dose dependent way. The IC 30 (mg·L -1 ) of PSO and 8 MOP were 32.4 and 25.1 and IC 50 (mg·L -1 ) 44.7 and 35.5 respectively. After the cells had been treated with the drugs at IC 30 for 5 d, the doubling time (h) for the control, PSO treated and 8 MOP treated cells were 20.8, 23.3 and 57.8 respectively, the percentage of S phase cells 24.5,17.8 and 5.8, the wild type p53 expression (%) 24.0,99.8 and 99.0, the nm23 H 1 expression (%) 99 9,99.5 and 99.1, the clonogenesity (%) 23.5,16.5 and 0, the number of metastatic foci on lung surface 139±61,114±68 and 36±32, respectively. CONCLUSION Both PSO and 8 MOP at the dosage of IC 30 may inhibit the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells, but 8 MOP is more effective.展开更多
文摘AIM To study the effects of psoralen (PSO) and 8 methoxypsoralen (8 MOP) on the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells. METHODS Inhibitory effects of PSO and 8 MOP on the proliferation and metastatic potential of mucoepidermaid carcinoma Mc3 cells were investigated with MTT assay, cell counting, flow cytometry and tail vein injection of the cells into nude mice (10 6 for each). RESULTS PSO and 8 MOP inhibited Mc3 cell growth in a dose dependent way. The IC 30 (mg·L -1 ) of PSO and 8 MOP were 32.4 and 25.1 and IC 50 (mg·L -1 ) 44.7 and 35.5 respectively. After the cells had been treated with the drugs at IC 30 for 5 d, the doubling time (h) for the control, PSO treated and 8 MOP treated cells were 20.8, 23.3 and 57.8 respectively, the percentage of S phase cells 24.5,17.8 and 5.8, the wild type p53 expression (%) 24.0,99.8 and 99.0, the nm23 H 1 expression (%) 99 9,99.5 and 99.1, the clonogenesity (%) 23.5,16.5 and 0, the number of metastatic foci on lung surface 139±61,114±68 and 36±32, respectively. CONCLUSION Both PSO and 8 MOP at the dosage of IC 30 may inhibit the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells, but 8 MOP is more effective.
