Background: Oxidative stress has been closely linked to the incidence of diabetic complications. Therefore, the aim of this research article was to study hyperglycemia and abnormal lipid profile in diabetic patient ty...Background: Oxidative stress has been closely linked to the incidence of diabetic complications. Therefore, the aim of this research article was to study hyperglycemia and abnormal lipid profile in diabetic patient type 2 and its correlation with oxidative stress development as measured by 8-iso-PGF2α and 8-OHdG. Methods: Fifty (50) patients confirmed type 2 diabetes mellitus and eighty (80) non-diabetic control individuals were included in this study. All individuals were tested for blood glucose, lipid profile, 8-iso-PGF2α and 8-OHdG HdG. Results: The age of diabetic patients was observed to be ≥40 yrs in 96% and diabetes was frequently detected in female than in male patients (76% vs. 24%, p ere elevated in diabetic patients compared with control individuals (p < 0.0001) except in HDL-C, a significant decrease was recorded (p = 0.04). Serum 8-iso-PGF2α and 8-OHdG were elevated significantly in diabetic patients compared with non-diabetic control and a significant correlation was recorded between them (r = 0.6, p α was associated with Age (r = 0.394, p < 0.0001), FBG (0.553, p < 0.0001), LDL-C (r = 0.2, p = 0.023), TG (r = 0.176, p = 0.045) and TC (r = 0.2, p = 0.02). Also, 8-OHdG was associated with age (r = 0.558, p < 0.0001), FBG (r = 0.67, p < 0.0001), LDL-C (r = 0.28, p = 0.001), TG (r = 0.358, p < 0.0001) and TC (r = 0.33, p < 0.0001). Age, FBG, HbA1c, LDL-C, TG and TC showed a significant linear regression with 8-iso-PGF2α and 8-OHdG recording its role as significant predictors for the elevation of 8-iso-PGF2α and 8-OHdG. Therefore, hyperglycemia with oxidative stress development may play a role for dyslipidemia and diabetic complications. Conclusion: Diabetic patient’s type 2 has a higher rate of abnormal serum lipids and correlates significantly with lipid peroxidation and oxidized DNA bases as measured by 8-iso-PGF2α and 8-OHdG. Therefore, 8-iso-PGF2α and 8-OHdG could be used as oxidative biomarkers for evaluating diabetic patients with early prediction of its complications and cancer development.展开更多
目的：探讨胎儿窘迫伴（或不伴）羊水粪染的新生儿脐血中8-异前列腺素（8-iso-PGF2α）水平的变化及其临床意义。方法：选取胎儿窘迫伴（或不伴）羊水粪染的新生儿152例,进行出生后1 min Apgar评分,按羊水粪染情况分为无粪染组36例和Ⅰ...目的：探讨胎儿窘迫伴（或不伴）羊水粪染的新生儿脐血中8-异前列腺素（8-iso-PGF2α）水平的变化及其临床意义。方法：选取胎儿窘迫伴（或不伴）羊水粪染的新生儿152例,进行出生后1 min Apgar评分,按羊水粪染情况分为无粪染组36例和Ⅰ度粪染组38例、Ⅱ度粪染组39例、Ⅲ度粪染组39例,并选取同期正常新生儿38例为对照组。采用双抗体夹心酶联免疫法检测五组新生儿脐血8-iso-PGF2α水平并对结果进行分析。结果：（1）胎儿窘迫病情越严重,患儿血清8-iso-PGF2α水平越高,体内氧化应激反应越严重,特别是羊水粪染≥Ⅱ度时,患儿血清8-iso-PGF2α水平明显高于正常新生儿、无或Ⅰ度粪染患儿（P〈0.05）;（2）Apgar评分≤8分患儿血清8-iso-PGF2α水平高于正常新生儿及9~10分患儿（P〈0.05）;（3）以8-iso-PGF2α水平为检验变量,是否窘迫为状态变量,作ROC曲线分析,曲线下面积（AUC）为0.880,95%CI为0.835-0.901,以8-iso-PGF2α水平判断是否有胎儿窘迫的Cut-off值为4.23 ng/m L。结论：胎儿窘迫的发生发展与过氧化损伤有关,脐血8-iso-PGF2α水平能够准确反映胎儿窘迫羊水粪染新生儿过氧化损伤的程度,与Apgar评分能够共同作为及时预测新生儿缺血缺氧性脑病发生的指标。展开更多
文摘Background: Oxidative stress has been closely linked to the incidence of diabetic complications. Therefore, the aim of this research article was to study hyperglycemia and abnormal lipid profile in diabetic patient type 2 and its correlation with oxidative stress development as measured by 8-iso-PGF2α and 8-OHdG. Methods: Fifty (50) patients confirmed type 2 diabetes mellitus and eighty (80) non-diabetic control individuals were included in this study. All individuals were tested for blood glucose, lipid profile, 8-iso-PGF2α and 8-OHdG HdG. Results: The age of diabetic patients was observed to be ≥40 yrs in 96% and diabetes was frequently detected in female than in male patients (76% vs. 24%, p ere elevated in diabetic patients compared with control individuals (p < 0.0001) except in HDL-C, a significant decrease was recorded (p = 0.04). Serum 8-iso-PGF2α and 8-OHdG were elevated significantly in diabetic patients compared with non-diabetic control and a significant correlation was recorded between them (r = 0.6, p α was associated with Age (r = 0.394, p < 0.0001), FBG (0.553, p < 0.0001), LDL-C (r = 0.2, p = 0.023), TG (r = 0.176, p = 0.045) and TC (r = 0.2, p = 0.02). Also, 8-OHdG was associated with age (r = 0.558, p < 0.0001), FBG (r = 0.67, p < 0.0001), LDL-C (r = 0.28, p = 0.001), TG (r = 0.358, p < 0.0001) and TC (r = 0.33, p < 0.0001). Age, FBG, HbA1c, LDL-C, TG and TC showed a significant linear regression with 8-iso-PGF2α and 8-OHdG recording its role as significant predictors for the elevation of 8-iso-PGF2α and 8-OHdG. Therefore, hyperglycemia with oxidative stress development may play a role for dyslipidemia and diabetic complications. Conclusion: Diabetic patient’s type 2 has a higher rate of abnormal serum lipids and correlates significantly with lipid peroxidation and oxidized DNA bases as measured by 8-iso-PGF2α and 8-OHdG. Therefore, 8-iso-PGF2α and 8-OHdG could be used as oxidative biomarkers for evaluating diabetic patients with early prediction of its complications and cancer development.
文摘目的：探讨胎儿窘迫伴（或不伴）羊水粪染的新生儿脐血中8-异前列腺素（8-iso-PGF2α）水平的变化及其临床意义。方法：选取胎儿窘迫伴（或不伴）羊水粪染的新生儿152例,进行出生后1 min Apgar评分,按羊水粪染情况分为无粪染组36例和Ⅰ度粪染组38例、Ⅱ度粪染组39例、Ⅲ度粪染组39例,并选取同期正常新生儿38例为对照组。采用双抗体夹心酶联免疫法检测五组新生儿脐血8-iso-PGF2α水平并对结果进行分析。结果：（1）胎儿窘迫病情越严重,患儿血清8-iso-PGF2α水平越高,体内氧化应激反应越严重,特别是羊水粪染≥Ⅱ度时,患儿血清8-iso-PGF2α水平明显高于正常新生儿、无或Ⅰ度粪染患儿（P〈0.05）;（2）Apgar评分≤8分患儿血清8-iso-PGF2α水平高于正常新生儿及9~10分患儿（P〈0.05）;（3）以8-iso-PGF2α水平为检验变量,是否窘迫为状态变量,作ROC曲线分析,曲线下面积（AUC）为0.880,95%CI为0.835-0.901,以8-iso-PGF2α水平判断是否有胎儿窘迫的Cut-off值为4.23 ng/m L。结论：胎儿窘迫的发生发展与过氧化损伤有关,脐血8-iso-PGF2α水平能够准确反映胎儿窘迫羊水粪染新生儿过氧化损伤的程度,与Apgar评分能够共同作为及时预测新生儿缺血缺氧性脑病发生的指标。
