An endophytic fungus producing 1,8-cineole from Neolitsea pulchella (Meissn.) Merr. was identified as Annulohypoxylon sp. by phylogenetic analyses of the sequence alignments of ITS rDNA, β-tubulin, Actin and EF1-α. ...An endophytic fungus producing 1,8-cineole from Neolitsea pulchella (Meissn.) Merr. was identified as Annulohypoxylon sp. by phylogenetic analyses of the sequence alignments of ITS rDNA, β-tubulin, Actin and EF1-α. This isolate produces an attractive spectrum of volatile organic compounds (VOCs) with only one dominant component, 1,8-cineole, as identified by gas chromatography-mass spectrometry (GC-MS). The fungus was able to grow in seven media with different carbon sources, and five raw agro-forest residues. The content of 1,8-cineole in the mixed VOCs via fungus reached up to 94.95% and 91.25% relative area in PDA and raw poplar sawdust, respectively. Under optimum test conditions, the fungus produced 1,8-cineole at the 0.764 ppmv in 50 mL head spaces in PDA. Interestingly, 1,8-cineole is an ideal fuel additive for both diesel and gasoline engines. Also, this is the first isolate, in this group of fungi, making cineole, which produces as its primary VOC product which makes it an ideal organism for strain improvement. Such as step will be critical for its ultimate use in biofuel production.展开更多
A supercritical CO2 extraction behavior of Eucalyptus oil was investigated under different conditions of pressure, temperature and time with or without cosolvent. The pressure range was from 8 to 25 MPa, temperature f...A supercritical CO2 extraction behavior of Eucalyptus oil was investigated under different conditions of pressure, temperature and time with or without cosolvent. The pressure range was from 8 to 25 MPa, temperature from 35 to 55°C and CO2 flow rate from 10 to 26 g/min. For 1,8-cineole the appropriate extracting pressure was 15 MPa and temperature was 45°C. When CO2 flow rate was 18 g/min, it was benefit to extract the other three substances (limonene, p-cymene and γ-terpinene, respectively) except 1,8-cineole. Prolonging extraction time could not obviously increase the extract concentration, but the extract yield would increase. The results also indicated that ethanol as a modifier could improve extraction velocity and extraction concentration.展开更多
1,8-cineole, also known as eucalyptol, is the main constituent and the most important of the leaf oils of many species of the genus Eucalyptus. In present study, the essential oils isolated by hydrodistillation from t...1,8-cineole, also known as eucalyptol, is the main constituent and the most important of the leaf oils of many species of the genus Eucalyptus. In present study, the essential oils isolated by hydrodistillation from the leaves of Eucalyptus camaldulensis from six different locations were analyzed by GC-FID and GC-MS. Essential oil yields ranged from 0.33% to 4.35%. In total, 34 constituents were identified, accounting for 94.0% to 99.8% of the total compositions. 1,8-cineole (46.4% - 84.4%) was the main constituent of all the essential oil samples, except for one sample collected in the Fatick region, which had p-cymene (46.4%) and 1,8-cineole (26.0%) as major compounds. The essential oil showed excellent activity against S. aureus, E. coli and E. faecalis (IZ = 25.3 ± 1.2 mm;18.7 ± 0.6 mm;17.8 ± 0.3 mm, respectively) and moderate activity against P. aeruginosa (IZ = 10.8 ± 0.8 mm). They may have potential applications in food and pharmaceutical products.展开更多
The oils extracted by hydro distillation from the aerial parts of Artemisia scoparia waldst. & kitag growing wild in two regions on Qinghai-Tibetan Plateau were analyzed by GC-MS. Eighty-three components were iden...The oils extracted by hydro distillation from the aerial parts of Artemisia scoparia waldst. & kitag growing wild in two regions on Qinghai-Tibetan Plateau were analyzed by GC-MS. Eighty-three components were identified representing 97.5% of the total components detected. The major constituents of the oil from the samples obtained in the eastern of Riyue Mountain (2700 - 3200 m) were 2-ethenyl-naphthalene (45.1%), beta-pinene (11.2%), 3-carene (8.7%), 3,7-dimethyl-1,3,6- octatriene (7.9%), limonene (5.4%), alpha-pinene (3.5%) and beta-myrcene (2.0%). Whereas the oil from the plant collected in Qilian Mountain (3300 - 3500 m) was composed mainly of thujone (21.4%), 1,8-cineole (18.9%), camphor (9.1%), 4-methyl-1-(1-methyl ethyl)-3-cyclo hexen-1-ol (7.8%), 4-methyl-1-(1-methylethyl)-bicyclo[3.1.0]hexan-3-one (5.3%) and 2-isopropyl-5-methyl- 3-cyclohexen-1-one(5.0%).展开更多
文摘An endophytic fungus producing 1,8-cineole from Neolitsea pulchella (Meissn.) Merr. was identified as Annulohypoxylon sp. by phylogenetic analyses of the sequence alignments of ITS rDNA, β-tubulin, Actin and EF1-α. This isolate produces an attractive spectrum of volatile organic compounds (VOCs) with only one dominant component, 1,8-cineole, as identified by gas chromatography-mass spectrometry (GC-MS). The fungus was able to grow in seven media with different carbon sources, and five raw agro-forest residues. The content of 1,8-cineole in the mixed VOCs via fungus reached up to 94.95% and 91.25% relative area in PDA and raw poplar sawdust, respectively. Under optimum test conditions, the fungus produced 1,8-cineole at the 0.764 ppmv in 50 mL head spaces in PDA. Interestingly, 1,8-cineole is an ideal fuel additive for both diesel and gasoline engines. Also, this is the first isolate, in this group of fungi, making cineole, which produces as its primary VOC product which makes it an ideal organism for strain improvement. Such as step will be critical for its ultimate use in biofuel production.
文摘A supercritical CO2 extraction behavior of Eucalyptus oil was investigated under different conditions of pressure, temperature and time with or without cosolvent. The pressure range was from 8 to 25 MPa, temperature from 35 to 55°C and CO2 flow rate from 10 to 26 g/min. For 1,8-cineole the appropriate extracting pressure was 15 MPa and temperature was 45°C. When CO2 flow rate was 18 g/min, it was benefit to extract the other three substances (limonene, p-cymene and γ-terpinene, respectively) except 1,8-cineole. Prolonging extraction time could not obviously increase the extract concentration, but the extract yield would increase. The results also indicated that ethanol as a modifier could improve extraction velocity and extraction concentration.
文摘1,8-cineole, also known as eucalyptol, is the main constituent and the most important of the leaf oils of many species of the genus Eucalyptus. In present study, the essential oils isolated by hydrodistillation from the leaves of Eucalyptus camaldulensis from six different locations were analyzed by GC-FID and GC-MS. Essential oil yields ranged from 0.33% to 4.35%. In total, 34 constituents were identified, accounting for 94.0% to 99.8% of the total compositions. 1,8-cineole (46.4% - 84.4%) was the main constituent of all the essential oil samples, except for one sample collected in the Fatick region, which had p-cymene (46.4%) and 1,8-cineole (26.0%) as major compounds. The essential oil showed excellent activity against S. aureus, E. coli and E. faecalis (IZ = 25.3 ± 1.2 mm;18.7 ± 0.6 mm;17.8 ± 0.3 mm, respectively) and moderate activity against P. aeruginosa (IZ = 10.8 ± 0.8 mm). They may have potential applications in food and pharmaceutical products.
文摘The oils extracted by hydro distillation from the aerial parts of Artemisia scoparia waldst. & kitag growing wild in two regions on Qinghai-Tibetan Plateau were analyzed by GC-MS. Eighty-three components were identified representing 97.5% of the total components detected. The major constituents of the oil from the samples obtained in the eastern of Riyue Mountain (2700 - 3200 m) were 2-ethenyl-naphthalene (45.1%), beta-pinene (11.2%), 3-carene (8.7%), 3,7-dimethyl-1,3,6- octatriene (7.9%), limonene (5.4%), alpha-pinene (3.5%) and beta-myrcene (2.0%). Whereas the oil from the plant collected in Qilian Mountain (3300 - 3500 m) was composed mainly of thujone (21.4%), 1,8-cineole (18.9%), camphor (9.1%), 4-methyl-1-(1-methyl ethyl)-3-cyclo hexen-1-ol (7.8%), 4-methyl-1-(1-methylethyl)-bicyclo[3.1.0]hexan-3-one (5.3%) and 2-isopropyl-5-methyl- 3-cyclohexen-1-one(5.0%).
