Model prediction of inactivation of Aeromonas salmonicida grown on poultry in situ by intense pulsed light

The aim of this study was to evaluate the factors influencing the inactivation effect of intense pulsed light(IPL)on Aeromonas salmonicida grown on chicken meat and skin,and to further develop prediction models of inactivation.In this work,chicken meat and skin inoculated with meat-borne A.salmonicida isolates were subjected to IPL treatments under different conditions.The results showed that IPL had obvious bactericidal effect in the chicken skin and thickness groups when the treatment voltage and time were 7 V combined with 5 s.In addition,the lethality curves of A.salmonicida were fitted under IPL conditions of 3.5-7.5 V.The comparison of statistical parameters revealed that the Weibull model could best fit the mortality curves and could accurately predict the mortality dynamic of A.salmonicida grown on chicken skin.And further a secondary model between the scale factor b and the treatment voltage in Weibull model was established using linear equations,which determined that the secondary model could accurately predict the inactivation of A.salmonicida.This study provides a theoretical basis for future prediction models of Aeromonas,and also provides new ideas for sterilization approaches of meat-borne Aeromonas.

鱼类致病杀鲑诺卡氏菌(Nocardia salmonicida)特异性PCR检测方法的建立

鱼类诺卡氏菌病是全球性的鱼病,杀鲑诺卡氏菌(Nocardia salmonicida)是引起鱼类诺卡氏菌病的主要病原之一。根据杀鲑诺卡氏菌16S-23S转录间隔区(ITS)序列设计引物,建立特异性PCR检测杀鲑诺卡氏菌的方法。结果表明,筛选的PCR引物可特异性地扩增出杀鲑诺卡氏菌的ITS片段,检测灵敏度(DNA浓度)为28.3 pg·μL-1。检测人工感染杀鲑诺卡氏菌的鱼组织,结果显示,该方法可从未分离到病原菌的病鱼组织中检出阳性片段,较传统细菌分离鉴定方法更为高效灵敏。

AI产业化与产业AI化:AI大模型产业生态的行动实践、变革趋势与前沿议题

以ChatGPT、Sora等为代表的AI大模型产业生态雏形正加速显现,但对其产业行动实践概况及其影响变革趋势的研究仍不够深入。文章创新性地提出AI大模型产业生态理念,从AI产业化与产业AI化两个维度并结合多个视角深入探究AI大模型产业生态体系。结果发现AI大模型产业生态呈现AI产业化与产业AI化共生、AI原生应用与“X+AI应用”并进、通用与专用模型共存、大模型与小模型协同、开源与闭源并行、多模态融合、端云AI混合的演化态势,且AI大模型正成为产业新型基础设施,促进手工作坊、分布式智能服务生产走向工业规模化、集中式智能服务生产,重塑人机关系、颠覆产业知识模型建构与调用范式并助推AI平民化、AIGS、具身智能、物理AI、超级AI应用等加速到来。在此基础上,从AI大模型产业生态体系构建及其分工协作机制、产业生态的不同路线选择及协同共生机制、AI大模型对传统产业赋能机制、产业生态治理体系等方面提出前沿议题展望。

SZEG TYPE FACTORIZATION THEOREM FOR NONCOMMUTATIVE HARDY-LORENTZ SPACES

We introduce noncommutative Hardy-Lorentz spaces and give the Szegō and inner-outer type factorizations of these spaces.

Surface Graft Copolymerization of Acrylic Acid onto Corona Treated Cotton Fabric

Acrylic acid was grafted onto the surface of cotton fabric after being short time treated by corona-discharge inair in the presence of initiator.The means of gas-phaseSO2 derivatization was used along with ESCA to deter-mine corona-discharge-induced-hydroperoxidegroups on the surface.The content of hydroperoxideshows a maxmium value at 15 sec.of corona-dischargetime.Effect of corona treatment time and various con-centration initlator on graft yield was studied.The addit-ion of initiator increases the graft yield.Acceleratedgraft with an increase in the concentration of Mohr’s saltshows that peroxide groups on the corona treated cottonfabric initiate graft copolymerization.

Efficient multiuser detector based on box-constrained deregularization and its FPGA design

Multiuser detection can be described as a quadratic optimization problem with binary constraint. Many techniques are available to find approximate solution to this problem. These tech- niques can be characterized in terms of complexity and detection performance. The ＂efficient frontier＂ of known techniques include the decision-feedback, branch-and-bound and probabilistic data association detectors. The presented iterative multiuser detection technique is based on joint deregularized and box-constrained so- lution to quadratic optimization with iterations similar to that used in the nonstationary Tikhonov iterated algorithm. The deregulari- zation maximizes the energy of the solution, this is opposite to the Tikhonov regularization where the energy is minimized. However, combined with box-constraints, the deregularization forces the solution to be close to the binary set. We further exploit the box- constrained dichotomous coordinate descent （DCD） algorithm and adapt it to the nonstationary iterative Tikhonov regularization to present an efficient detector. As a result, the worst-case and aver- age complexity are reduced down to K28 and K2~ floating point operation per second, respectively. The development improves the ＂efficient frontier＂ in multiuser detection, which is illustrated by simulation results. Finally, a field programmable gate array （FPGA） design of the detector is presented. The detection performance obtained from the fixed-point FPGA implementation shows a good match to the floating-point implementation.

Immuno-Protective Efficiency of the Bivalent Inactivated Vaccine Against Vibrio scophthalmi and Aeromonas salmonicida Infections in Turbot(Scophthalmus maximus L.)

Vibrio scophthalmi and Aeromonas salmonicida can cause high turbot mortality and huge economic losses.Presently,vaccination is the most promising method for preventing communicable diseases.In this study,we used formalin to kill V.scophthalmi and A.salmonicida cells,and mixed with the mineralized oil adjuvant(Montanide^(TM)ISA 763 AVG)to prepare the bivalent inactivated vaccine.The results showed that turbot inoculated with the bivalent inactivated vaccine exhibited strong tolerance to the infection of V.scophthalmi and A.salmonicida,and no obvious clinical symptoms and pathological changes were observed.The activities of enzymes lysozyme,acid phosphatase and complement C3 had significantly increased after the vaccination.The antibody titer response of vaccinated turbot was greatly boosted,which was positively connected with the immunological impact according to ELISA results.Simultaneously,the expression levels of immune-related genes such as MHC-IIα,MHC-IIβ,CD4,CD8,TNF-αand IL^(-1)βwere up-regulated,demonstrating that it might stimulate humoral and cellular immunological response in turbot.These findings highlight the potential of the bivalent inactivated vaccine for controlling V.scophthalmi and A.salmonicida infections in turbot.

Concentration of Enrofloxacin Residue from Tilapia （Oreochromis niloticus） Muscular That Infected by Aeromonas salmonicida

Aim of this research was to find out the concentration of enrofloxacin residue in tilapia meat for several weeks after antibiotic treatment. Twenty seven tilapia fishes were divided into three groups. The first group was not infected and treated, the second group was infected with A. salmonicida subsp, smithia and the third group was infected with A. salmonicida subsp. achromogenes intramuscularly. Six days after infection, treatment was carried out using Baytril administered orally for the second group and intramuscularly for the third group during five days. At the 1 st, 4th and 8th week after the treatment, Three fish were taken from each group to be analyzed for its concentration of enrofloxacin residue by diffusion on Mueller Hinton Agar （MHA） method and quantitatively using high performance liquid chromatography （HPLC） method. The MHA test showed the formation of inhibition zone, at the 1 st week and 4th week after the treatment, while at 8th week after treatment did not show inhibition zone. The HPLC test on enrofloxacin residual concentration in tilapia infected with A. salmonicida subsp, smithia （second group） at the 1st, 4th and 8th week after treatment showed the average of 33.0, 6.10 and 0.0021 μg/g of enrofloxacin residue level. While in tilapia infected with A. salmonicida subsp, achromogenes and treated with enrofloxacin intramuscularly （third group） showed the average of residue level 35.79, 2.18 and 0.00065 μg/g. In conclusion, the residue of enrofloxacin was still high concentration until the fourth week after treatment in the second and third groups. Based on Indonesian National Standards and Rules, the maximum limit of enrofloxacin residue is 0.01 μg/g. The concentration of enrofloxacine residue was very low and the concentration of enrofloxacin residue collected from tilapia using orally and intramuscularly method of treatment was not different.

基于BaMgSiO_(4)氧空位缺陷发光的可视化荧光温度传感材料

温度是表征物理化学性质的最基本参数之一,精确的温度测量对于现代科学技术发展起着至关重要的作用。传统基于稀土离子热耦合能级对(TCLs)能量传递的荧光温度传感器因TCLs之间能量差的限制存在测温灵敏度低及信号区分困难等问题。为寻求更优的解决方案,本研究探索了氧空位缺陷发光在荧光温度传感器领域的应用前景。本文通过高温固相法合成了BaMgSiO_(4)陶瓷,由于在高温烧结过程中有少量Ba^(2+)和Mg^(2+)蒸发,陶瓷中会产生氧空位以保持材料电中性。这些氧空位所形成的缺陷能级在332 nm紫外光激发下,发射出372,400,527 nm三种波长的发射光。这三种发射光强度对温度有着不同的敏感性,使得其能够良好应用于荧光温度传感领域。其中,I372和I527组成的温度传感系统相对测温灵敏度在298 K时为2.90%·K^(-1),高于传统TCLs荧光温度传感器的测温灵敏度,突破了TCLs温度传感器的灵敏度天花板。另外,由于372 nm和527 nm波长相差较大,使得BaMgSiO_(4)陶瓷有着室温下绿光发射到458 K高温下蓝光发射的显著变化,实现了温度监控可视化。因此,BaMgSiO_(4)陶瓷因其独特的氧空位缺陷发光特性,为开发荧光温度传感器提供了一种高精度和可视化的新选择,为荧光温度探测技术提供了一条新思路。

经dTRA、常规TRA穿刺置管在冠脉和脑血管“一站式”造影检查中的应用对比观察

目的对比观察经远端桡动脉入路(d TRA)、常规桡动脉入路(TRA)穿刺置管在冠脉和脑血管“一站式”造影检查中的应用效果。方法择期行冠脉和脑血管“一站式”造影检查的受检者49例,随机分为d TRA 24例、TRA组25例,d TRA受检者采用经d TRA穿刺置管进行冠脉和脑血管“一站式”造影检查,TRA组采用经TRA穿刺置管进行冠脉和脑血管“一站式”造影检查。观察并比较两组受检者穿刺置管成功率、穿刺置管时间、手术时间、X射线曝光时间、术中累计放射总量、造影剂用量、压迫止血时间、术后食指血氧饱和度、术后住院时间、术后第1天VAS评分、术后第3天VAS评分、住院患者满意度等手术相关资料,以及术后出现迷走神经反射、穿刺点出血、右手及前臂张力性血肿、桡神经损伤、桡动脉闭塞、桡动脉痉挛、皮肤溃烂等并发症发生情况。结果d TRA组受检者穿刺置管时间、住院患者满意度均高于TRA组,术后第1天VAS评分、术后第3天VAS评分、压迫止血时间均低于TRA组(P均<0.05)。两组受检者均未出现迷走神经反射、穿刺点出血、右手及前臂张力性血肿、桡神经损伤、桡动脉闭塞、桡动脉痉挛、皮肤溃烂等相关并发症。结论与经TRA穿刺置管进行冠脉和脑血管“一站式”造影检查相比,经d TRA穿刺置管进行冠脉和脑血管“一站式”造影检查术后疼痛减轻、压迫止血时间缩短,患者满意度更高,可作为冠脉和脑血管“一站式”造影检查的常规入路之一。

A TRUST REGION ALGORITHM FOR A CLASS OF NONLINEAR OPTIMIZATION

This paper presents a trust region algorithm for nonlinear optimization with linear inequality constraints. The global convergence of the algorithm is proved. Local quadratic convergence is obtained for a strong local minimizer.

基于PDCA循环法优化病区药房手摆药品管理

目的基于PDCA循环法,找出病区药房手摆药品在调剂接收过程中存在的问题,并采用信息化手段进行优化实践。方法选定降低病区药房手摆药品调剂接收产生的差错率为衡量指标,利用鱼骨图、柏拉图、“80/20”法则分析产生差错原因,并进行信息化优化实践。结果病区药房手摆药品在调剂转运接收过程中的差错率由0.28%降至0.12%,进步率为57.14%。结论应用PDCA循环法有效地优化了手摆药品从调剂转运接收全流程,保障临床诊疗用药安全以及促进了病区药房手摆药品信息化管理的发展。

改进NSGA-Ⅱ算法的新能源储能系统协调优化模型

新能源储能系统的分散负荷难以调度,导致整体协调效果较差,为促进新能源并网消纳,构建了改进NSGA-Ⅱ算法的新能源储能系统协调优化模型。设计新能源储能系统拓扑结构,使用NSGA-II算法定义协调目标拥挤度为储能动态调节参数。以新能源储能削减计划偏差最小为目标函数进行参数寻优,并确定转移容量指标和负荷平衡指标。在NSGA-II算法中引入收缩扩张系数,保证分散负荷资源搜索多样性,采用改进NSGA-II算法,由此构建协调优化模型。实验结果表明,该模型协调优化后光伏储能功率为43 MW,风电储能功率为65 MW,储热储能功率为16 MW,有效促进了新能源并网消纳。

绿色纳米碳酸钙生产关键技术与机理研究

以电石渣等钙镁废渣为原料来制备绿色纳米碳酸钙的生产工艺简单、设备不多、投资不大,属于“短平快”项目和新质生产力项目。采用离子计和pH计来跟踪研究浸取反应,并对新鲜浸取液和循环母液浸取反应过程的钙离子浓度、电位值、pH与时间变化的关系进行了比较。新鲜浸取液反应速度快、时间短、浸取率高;循环母液的浸取反应速度较慢、时间较长、浸取率较低。预碳化是一个极快的复分解反应过程,中间产物呈胶体状,其反应量约占钙离子总量的20%左右,目的是为CO_(2)碳化反应提供晶种;采用离子计和pH计来跟踪研究CO_(2)碳化反应过程,发现前期速度较慢,CO_(2)的气液平衡是控制步骤,后期氨的电离平衡是控制步骤,停止通入CO_(2)后碳化过程并没有立即停止,钙离子浓度和电位值继续下降,但pH有小幅回升。通过对电石渣浸取反应和氯化钙-氨水体系CO_(2)碳化机理的深入研究,为绿色纳米碳酸钙的产业化之路奠定了基础。

养殖裸盖鱼(Anoplopoma fimbria)疥疮病病原菌的分离与鉴定

裸盖鱼(Anoplopoma fimbria)是一种名贵的冷水经济鱼类,已在我国北方地区开展小规模的工厂化繁育和养成。目前,我国关于裸盖鱼的病害研究仍是空白。本研究对山东烟台一养殖场自然发生疥疮病的裸盖鱼进行了病原分析,从发病鱼体内分离得到形态一致的优势菌株,命名为AF-1,并对其进行了致病性检测、菌种鉴定及药物敏感性研究。人工感染实验证明,AF-1对裸盖鱼有致病性,呈现症状与自然发病状态一致;结合形态观察、革兰氏染色、生理生化特征、16S r RNA和gyr B基因序列进化树分析,将AF-1鉴定为杀鲑气单胞菌(Aeromonas salmonicida);药物敏感性实验结果显示,AF-1对青霉素、阿莫西林、头孢噻吩等13种抗生素具有耐药性,对氟苯尼考、氟甲喹等16种抗生素敏感。综上所述,本研究首次报道了我国养殖裸盖鱼感染杀鲑气单胞菌病例,为裸盖鱼养殖过程中的疾病防控和疫苗开发提供了参考。

羊源杀鲑气单胞菌16S rRNA基因的克隆测序及分析

从重庆市丰都县无菌采集山羊血液,提取全血基因组,用16SrRNA基因的引物进行PCR扩增,得到2条长约1 500bp的扩增片段,将其克隆到pMD19-T载体后进行测序,并与3条豚鼠气单胞菌,3条嗜水气单胞菌,3条中间气单胞菌,4条杀鲑气单胞菌,2条温和气单胞菌,3条维氏气单胞菌和5条舒氏气单胞菌16SrRNA基因序列进行系统发育分析.结果表明:所克隆的基因片段长度均为1 507bp,GenBank登录号为JF823571和JF823572.系统发育分析发现2条序列均被聚类到杀鲑气单胞菌群,并与温和气单胞菌聚类到一个大的分支.同源性比对结果显示所获得的序列与杀鲑气单胞菌法国株(ATCC33658T)X74681和阿根廷株AF134065的同源性最高,均为99.6%,表明重庆地区存在受杀鲑气单胞菌感染的山羊.

硫代硫酸铵预硫化的MoO3／Al2O3催化剂的活化和加氢脱硫活性

以硫代硫酸铵为硫化剂对MoO3／Al2O3催化剂进行预硫化，考察了制备方法和活化条件对预硫化催化剂噻吩加氢脱硫活性的影响．结果表明，硫代硫酸铵预硫化的催化剂活化后，加氢脱硫活性好，噻吩的转化率达到99％以上，而二甲基二硫硫化的MoO3／Al2O3催化剂在相同条件下，噻吩转化率只有92％．合适的活化温度为200～300℃，活化压力增加有利于预硫化催化剂的还原硫化和加氢脱硫活性的提高．硫代硫酸铵预硫化催化剂的高脱硫活性主要归因于多层的Ⅱ型MoS2活性相的形成，其次是硫化程度的提高．硫代硫酸铵预硫化催化剂经过氢气活化和补充硫化两个阶段，其硫化程度高于传统方法硫化的催化剂．

实时荧光定量PCR扩增特异性vapA基因检测杀鲑气单胞菌

为实现杀鲑气单胞菌早期快速准确定量检测,研究旨在建立杀鲑气单胞菌的SYBR Green Ⅰ实时荧光定量PCR(Real-time PCR)检测方法。根据GenBank中杀鲑气单胞菌毒力阵列蛋白基因(vapA)保守序列设计并合成一对特异性引物,对其特异性、灵敏度、可重复性和应用性进行评价。结果显示,研究设计的引物具有良好的种间特异性,仅对杀鲑气单胞菌及其亚种有阳性扩增,与其他细菌不发生交叉反应。构建的Real-time PCR标准曲线质粒拷贝数与循环阈值呈良好的线性关系,扩增所得标准曲线分别为y=–4.8345x+42.535,相关系数R^2为0.998,最低检测限为34拷贝/μL,较常规PCR的灵敏度高出约1000倍。应用建立的方法检测人工感染的虹鳟病样,15个被检样品呈阳性反应,与细菌常规鉴定方法结果一致。研究表明,所建立的基于实时荧光定量PCR技术的杀鲑气单胞菌检测方法快速、特异、灵敏,可用于临床诊断和疫病监测。

石鲽(Kareius bicoloratus L.)源杀鲑气单胞菌杀鲽亚种生物学性状的研究

采用分类方法,对从两起石鲽(KareiusbicoloratusL.)细菌性败血感染症病例的病(死)鱼中分离到的相应病原菌,进行形态特征、培养特征、自凝性、色素产生情况及生理生化特性等较系统的表观分类学指征鉴定及代表菌株DNA中(G+C)%的测定等研究。结果表明,分离菌为杀鲑气单胞菌的新亚种(subsp.nov.)并定名为杀鲑气单胞菌杀鲽亚种(Aeromonassalmonicidasubsp.flounderacidasubsp.nov.)。经血清型检定,表明60株菌均为同种血清型。选择代表菌株做对健康石鲽、牙鲆、鲤、鲫及泥鳅的人工感染试验,表明了相应的原发病原学意义及较强的致病作用。药敏试验结果表明,对供试37种抗菌药物中的青霉素G等6种耐药,对头孢噻肟等25种高敏,对多黏菌素B、利福平2种敏感,对头孢唑啉等4种在株间存在一定敏感性差异。该研究结果能较全面地反映出该菌的主要生物学性状。

基于MATLAB/SIMULINK的航空发动机建模与仿真研究

基于MATLAB/SIMULINK高级图形仿真环境,利用图形模块化技术开发了某型双轴涡扇发动机的通用部件级模型仿真系统.模型主要利用SIMULINK的模块库和S函数构建,提出了构建发动机模型的四层次结构,具有模型层次化、可封装模块化、面向结构图和高度可视化等特点.在SIMULINK中进行动态仿真,并用试验数据曲线验证模型的正确性.为发动机控制系统和故障诊断研究建立了一种灵活的仿真平台.