期刊文献+
共找到5,939篇文章
< 1 2 250 >
每页显示 20 50 100
Experimental Study on Towing Characteristics of Composite Bucket Wellhead Platform
1
作者 ZHANG Pu-yang LIU Ying-fei +2 位作者 LE Cong-huan DING Hong-yan BAI Yu 《China Ocean Engineering》 SCIE EI CSCD 2024年第3期531-542,共12页
With the rapid development of large-scale development of marginal oilfields in China,simple wellhead platforms that are simple in structure and easy to install have become an inevitable choice in the process of oilfie... With the rapid development of large-scale development of marginal oilfields in China,simple wellhead platforms that are simple in structure and easy to install have become an inevitable choice in the process of oilfield development.However,traditional simple wellhead platforms are often discarded after a single use.In pursuit of a more costeffective approach to developing marginal oilfields,this paper proposes a new offshore oil field development facility—an integrated bucket foundation for wellhead platform.To verify the safety of its towing behavior and obtain the dynamic response characteristics of the structure,this paper takes a bucket integrated bucket foundation for wellhead platform with a diameter of 40 m as the research object.By combining physical model tests and numerical simulations,it analyzes the static stability and dynamic response characteristics of the structure during towing,complete with the effects of the draft,wave height,wave period,and towing point height,which produce the dynamic responses of the structure under different influence factors,such as roll angle,pitch angle,heave acceleration and towing force as well as the sensibility to transport variables.The results show that the integrated bucket foundation for wellhead platform is capable of self-floating towing,and its movement is affected by the local environment,which will provide a reference for actual projects. 展开更多
关键词 composite bucket foundation wellhead platform air flotation towing model test
下载PDF
Scheme of Railway 5G Integrated Innovation Test Platform
2
作者 CHAI Jinchuan ZHENG Mingda 《Chinese Railways》 2023年第1期62-70,共9页
An inevitable trend has already taken shape for the application of the 5th Generation Mobile Communication Technology(5G)in the railway sector.The application scenarios and business types of the railway sector are com... An inevitable trend has already taken shape for the application of the 5th Generation Mobile Communication Technology(5G)in the railway sector.The application scenarios and business types of the railway sector are complex and diverse,so it is indispensable to test and verify the railway 5G before actual deployment.The design and creation of the railway 5G integrated innovation test platform provides engineering design,test and verification conditions for the networking,coverage and business development of 5G public networks and 5G-R in railway scenarios.This paper introduces the design of the overall architecture for the integrated railway 5G innovation test platform according to the railway network requirements,application scenarios and intelligent development trend;respectively elaborates on the design of the 5G-R core network,bearer network and wireless access along loop tracks,in combination with the characteristics of railway scenarios and the requirements of railway dispatching,operation and safety;raises further solutions on the network deployment and coverage schemes of 5G public networks so as to meet the application requirements of 5G public networks.The study results show that the integrated railway 5G innovation test platform scheme contains co-existence of 5G public and private networks,combines the indoor and outdoor scenarios,as well as takes into account of the dynamic and static tests so as to meet the environmental requirements for the integrated railway 5G test and application of all network functions,for which it can provide comprehensive technical support for railway 5G technology research and development,standard formulation,testing,etc. 展开更多
关键词 railway communication 5G public network 5G-R integrated innovation test platform
下载PDF
Control system for dynamic testing platform of asymmetrical pitch mechanism
3
作者 李恒宇 罗均 +1 位作者 谢少荣 郝山波 《Journal of Shanghai University(English Edition)》 CAS 2009年第4期276-278,共3页
A novel asymmetrical pitch system for rotary wing is presented. The pitch control characteristics are studied and analyzed. Because elastic linkage is a key part in whole asymmetrical pitch system, in order to obtain ... A novel asymmetrical pitch system for rotary wing is presented. The pitch control characteristics are studied and analyzed. Because elastic linkage is a key part in whole asymmetrical pitch system, in order to obtain the variation of the elastic linkage deformation, an experimental platform mainly based on the device of micro aerial vehicles (MAVs) and a new control system mounted on TMS320LF2407 are designed. This control system has its compacted configuration and reliability. Finally, using this system to control the MAV for simulating the flying forward, experimental results show the MAV's flight attitude can he controlled based on the variation of the elastic linkage. 展开更多
关键词 asymmetrical pitch micro aerial vehicle (MAV) experimental system dynamic testing platform
下载PDF
Development of a Remotely Controlled Testing Platform with Low-drag Air-ventilated Hull 被引量:3
4
作者 Konstantin I. Matveev Nicholaus I. Perry Alexander W. Mattson Christopher S. Chaney 《Journal of Marine Science and Application》 CSCD 2015年第1期25-29,共5页
这份报纸探讨发展并且有创新的通气空气的壳的一个遥远地控制的船平台严峻。轮船壳的下侧上的空气洞的申请是一答应为减少水动力学的工具拖和污染物质排出物和增加的海洋的交通效率。尽管有这个概念潜力,设计优化和新奇空气洞轮船的高... 这份报纸探讨发展并且有创新的通气空气的壳的一个遥远地控制的船平台严峻。轮船壳的下侧上的空气洞的申请是一答应为减少水动力学的工具拖和污染物质排出物和增加的海洋的交通效率。尽管有这个概念潜力,设计优化和新奇空气洞轮船的高效的操作仍然是一个挑战性的问题。赫尔建设和模型规模空气洞船的传感器乐器学在纸被描述。壳的模块化的结构允许容易的修正,和一个电的推进单位启用自己推动的操作。船经由一个收音机传播系统遥远地被控制。起始的测试的结果被报导,包括在几的戳,速度,和气流率装载条件。构造平台能被用于优化空气洞系统并且测试另外的创新的壳图案。这个系统能也被开发进一只高效的无人的船。 展开更多
关键词 测试平台 远程控制 低阻力 通风 空气 开发 船体结构 传输系统
下载PDF
Research on the Computer Software Testing Method Based on Multiple Platforms
5
作者 Yongfang Sun Jianjun Li 《Journal of Computer Science Research》 2020年第3期6-9,共4页
Informationization plays an important role in modern life and production.And various software is one of the bases for it.Before it goes into service,software needs to go through many steps,including software developme... Informationization plays an important role in modern life and production.And various software is one of the bases for it.Before it goes into service,software needs to go through many steps,including software development,design,etc.In software development,test is the key to identify and control bugs and errors in the software.Therefore,software companies often test the software to ensure that it is qualified.In recent years,more attention has been paid to a multi-platform computer software testing method,which can make up for defects in traditional testing methods to improve test accuracy.Firstly,this paper illustrates the connotation and features of software testing.Secondly,common software testing platforms and their requirements are analyzed.Finally,this paper proposes software testing method based on multiple platforms. 展开更多
关键词 Multiple platforms computer software testing method
下载PDF
First Systematic Testing Platform for Pressurization Feed System Developed for Liquid Rocket Propellant in China
6
作者 Zhang Yi Beijing Aerospace System Engineering Institute of CALT 《Aerospace China》 2011年第3期-,共1页
Beijing Aerospace System Engineering Institute of China Academy of Launch Vehicle Technology (CALT) declared recently that theinstitute has set up a laboratory whichwould operate a newly
关键词 cALT FEED First Systematic testing platform for Pressurization Feed System Developed for Liquid Rocket Propellant in china
下载PDF
超高温陶瓷改性C/C复合材料抗氧化烧蚀性能研究进展 被引量:1
7
作者 孙丹羽 朱世步 +2 位作者 刘泰山 张强 马荣檄 《材料导报》 EI CAS CSCD 北大核心 2024年第S01期104-114,共11页
碳/碳(C/C)复合材料具有高强、轻质和耐温等优势,在航天领域取得广泛应用;但由于其抗氧化性能较差,难以满足高超声速飞行器长时飞行、大气层再入飞行和跨大气层飞行的服役环境。利用超高温陶瓷改性C/C复合材料(C/C-UHTC)可以显著提高C/... 碳/碳(C/C)复合材料具有高强、轻质和耐温等优势,在航天领域取得广泛应用;但由于其抗氧化性能较差,难以满足高超声速飞行器长时飞行、大气层再入飞行和跨大气层飞行的服役环境。利用超高温陶瓷改性C/C复合材料(C/C-UHTC)可以显著提高C/C复合材料的抗氧化烧蚀性能,有望满足新一代飞行器长时间抗氧化、抗烧蚀及结构强度的要求。本文总结了C/C-UHTC复合材料烧蚀性能的常用测试方法,包括氧-乙炔烧蚀、等离子烧蚀、激光烧蚀和风洞烧蚀,简要介绍了各种烧蚀性能测试方法的特点。此外,对近年来Zr系和Hf系单组元、双组元和三组元C/C-UHTC复合材料的烧蚀性能及烧蚀机理进行详细阐述。 展开更多
关键词 超高温陶瓷 c/c复合材料 抗氧化烧蚀性能 测试方法 烧蚀机制
下载PDF
Current testing strategies for hepatitis C virus infection in blood donors and the way forward 被引量:9
8
作者 Neelam Marwaha Suchet Sachdev 《World Journal of Gastroenterology》 SCIE CAS 2014年第11期2948-2954,共7页
Screening tests for blood donations are based upon sensitivity,cost-effectiveness and their suitability for high-throughput testing.Enzyme immunoassay(EIAs)for hepatitis C virus(HCV)antibodies were the initial screeni... Screening tests for blood donations are based upon sensitivity,cost-effectiveness and their suitability for high-throughput testing.Enzyme immunoassay(EIAs)for hepatitis C virus(HCV)antibodies were the initial screening tests introduced.The"first generation"antibody EIAs detected seroconversion after unduly long infectious window period.Improved HCV antibody assays still had an infectious window period around 66 d.HCV core antigen EIAs shortened the window period considerably,but high costs did not lead to widespread acceptance.A fourth-generation HCV antigen and antibody assay(combination EIA)is more convenient as two infectious markers of HCV are detected in the same assay.Molecular testing for HCV-RNA utilizing nucleic acid amplification technology(NAT)is the most sensitive assay and shortens the window period to only4 d.Implementation of NAT in many developed countries around the world has resulted in dramatic reductions in transfusion transmissible HCV and relative risk is now<1 per million donations.However,HCV serology still continues to be retained as some donations are serology positive but NAT negative.In resource constrained countries HCV screening is highly variable,depending upon infrastructure,trained manpower and financial resource.Rapid tests which do not require instrumentation and are simple to perform are used in many small and remotely located blood centres.The sensitivity as compared to EIAs is less and wherever feasible HCV antibody EIAs are most frequently used screening assays.Efforts have been made to implement combined antigen-antibody assays and even NAT in some of these countries. 展开更多
关键词 HEPATITIS c VIRUS Screening tests Blood DONORS IMM
下载PDF
Site testing campaign for the Large Optical/infrared Telescope of China:general introduction of the Daocheng site 被引量:5
9
作者 Teng-Fei Song Yu Liu +8 位作者 Jing-Xing Wang Xue-Fei Zhang Shun-Qing Liu Ming-Yu Zhao Xiao-Bo Li Zhan-Chuan Cai Qi-Wu Song Zi-Huang Cao Yu Ruan 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2020年第6期151-161,共11页
The Daocheng site is one of the three candidate sites for the Large Optical/infrared Telescope(LOT)of China.It was discovered by Yunnan Observatories during the survey of potential sites for the next-generation large-... The Daocheng site is one of the three candidate sites for the Large Optical/infrared Telescope(LOT)of China.It was discovered by Yunnan Observatories during the survey of potential sites for the next-generation large-aperture solar telescopes of China.This paper describes the overview of the site,the observation platform and the monitor instrument.In addition,simple statistical results are presented(from November,2016 up to December,2017).Detailed data results can refer to the overview of LOT site testing and data analysis articles,which were published during the same period. 展开更多
关键词 site testing LOT Daocheng site observation platform
下载PDF
Recent progress on rapid diagnosis of COVID-19 by point-of-care testing platforms 被引量:1
10
作者 Ya-Nan Tang Dingding Jiang +2 位作者 Xuejun Wang Yunqi Liu Dacheng Wei 《Chinese Chemical Letters》 SCIE CAS CSCD 2024年第3期66-75,共10页
The outbreak of COVID-19 has drawn great attention around the world.SARS-CoV-2 is a highly infectious virus with occult transmission by many mutations and a long incubation period.In particular,the emergence of asympt... The outbreak of COVID-19 has drawn great attention around the world.SARS-CoV-2 is a highly infectious virus with occult transmission by many mutations and a long incubation period.In particular,the emergence of asymptomatic infections has made the epidemic even more severe.Therefore,early diagnosis and timely management of suspected cases are essential measures to control the spread of the virus.Developing simple,portable,and accurate diagnostic techniques for SARS-CoV-2 is the key to epidemic prevention.The advantages of point-of-care testing technology make it play an increasingly important role in viral detection and screening.This review summarizes the point-of-care testing platforms developed by nucleic acid detection,immunological detection,and nanomaterial-based biosensors detection.Furthermore,this paper provides a prospect for designing future highly accurate,cheap,and convenient SARS-CoV-2 diagnostic technology. 展开更多
关键词 cOVID-19 Point-of-care testing platforms Nucleic acid detection Asymptomatic infection Biosensors
原文传递
桂东北D/C界线沉积特征及对台盆相间演化的启示
11
作者 吴亮君 王璞珺 +11 位作者 吴继文 韦盛亮 户景松 吕勇 潘明 周嘉铭 林宇 廖家飞 韦伟 韦艾辰 李喜林 苏光样 《地质学报》 EI CAS CSCD 北大核心 2024年第4期1244-1262,共19页
台盆相间是桂东北泥盆纪和石炭纪沉积的重要特色,而D/C界线又是台盆相间沉积最突出的一个时段之一。文章回顾并评述了桂东北D/C界线的研究历程、相序划分、主要代表剖面,认为桂东北D/C界线可划分为碎屑岩相序和碳酸盐岩相序以及它们的... 台盆相间是桂东北泥盆纪和石炭纪沉积的重要特色,而D/C界线又是台盆相间沉积最突出的一个时段之一。文章回顾并评述了桂东北D/C界线的研究历程、相序划分、主要代表剖面,认为桂东北D/C界线可划分为碎屑岩相序和碳酸盐岩相序以及它们的过渡类型。通过对20条典型剖面的岩性组合特征对比,恢复了桂东北D/C界线海平面相对下降期的岩相古地理,结果显示桂东北在该时期主要存在柳州、桂林两大台地以及多个小型台地,台地间普遍发育快速相变的台沟、盆地沉积,石炭纪初期海水变浅,台地分布范围显著扩大,江南古陆供给增强,台盆相间格局迅速发展。为了进一步探讨桂东北D/C界线的演化过程和背后的控制因素,文章分析了广西整体的台盆相间演化的阶段性、同沉积构造作用以及海平面变化的影响,梳理了台盆相间演化过程中沉积环境分异与构造运动-海平面的关系,认为在滇黔桂裂谷盆地在持续断陷发展的前提下,桂东北D/C界线沉积分异的主要原因可能是受冈瓦纳大陆冰川形成与消融导致的海平面异常波动,以及柳江运动引发的局部同沉积断陷强度差异变动影响,且构造和沉积要素在D/C界线的耦合作用达到最强,最终促进桂东北台盆相间格局在D/C界线达到鼎盛。在此模式下,可以将台盆相间的演化划分为4个阶段,早泥盆世中期—晚期为台盆相间格局的孕育期,早泥盆世晚期—中泥盆世晚期沉积开始分异,为台盆相间格局的发展期,晩泥盆世—早石炭世台-盆出现强烈分割,海平面异常下降和柳江运动同时作用于沉积,为台盆相间格局的鼎盛期,早石炭世之后,台盆相间沉积维持了一定时期的强度,晚石炭世开始海水逐步恢复正常后台盆相间逐渐减弱,为台盆相间格局的衰退期。 展开更多
关键词 台盆相间 D/c界线 桂东北 广西 泥盆纪 石炭纪 古地理 柳江运动
下载PDF
Application of the monitoring and early warning system for internal solitary waves:Take the second natural gas hydrates production test in the South China Sea as an example
12
作者 Dan-yi Su Bin-bin Guo +5 位作者 Qian-yong Liang Chu-jin Liang Fei-long Lin Su-meng Jiang Yi-fei Dong Xue-min Wu 《China Geology》 CAS CSCD 2023年第4期676-684,共9页
Internal solitary waves(ISWs) contain great energy and have the characteristics of emergency and concealment. To avoid their damage to offshore engineering, a new generation of monitoring and early warning system for ... Internal solitary waves(ISWs) contain great energy and have the characteristics of emergency and concealment. To avoid their damage to offshore engineering, a new generation of monitoring and early warning system for ISWs was developed using technologies of double buoys monitoring, intelligent realtime data transmission, and automatic software identification. The system was applied to the second natural gas hydrates(NGHs) production test in the Shenhu Area, South China Sea(SCS) and successfully provided the early warning of ISWs for 173 days(from October 2019 to April 2020). The abrupt changes in the thrust force of the drilling platform under the attack of ISWs were consistent with the early warning information, proving the reliability of this system. A total of 93 ISWs were detected around the drilling platform. Most of them occurred during the spring tides in October–December 2019 and April 2020, while few of them occurred in winter. As suggested by the theoretical model, the full-depth structure of ISWs was a typical current profile of mode-1, and the velocities of wave-induced currents can reach 80 cm/s and30 cm/s, respectively, in the upper ocean and near the seabed. The ISWs may be primarily generated from the interactions between the topography and semidiurnal tides in the Luzon Strait, and then propagate westward to the drilling platform. This study could serve as an important reference for the early warning of ISWs for offshore engineering construction in the future. 展开更多
关键词 Internal solitary wave Early warning Offshore engineering Drilling platform Natural gas hydrates production test Shenhu Area South china Sea
下载PDF
Predictive potential of IL-28B genetic testing for interferon based hepatitis C virus therapy in Pakistan: Current scenario and future perspective 被引量:1
13
作者 Muhammad Sohail Afzal 《World Journal of Hepatology》 CAS 2016年第26期1116-1118,共3页
In Pakistan which ranked second in terms of hepatitis C virus(HCV) infection, it is highly needed to have an established diagnostic test for antiviral therapy responseprediction. Interleukin 28B(IL-28B) genetic testin... In Pakistan which ranked second in terms of hepatitis C virus(HCV) infection, it is highly needed to have an established diagnostic test for antiviral therapy responseprediction. Interleukin 28B(IL-28B) genetic testing is widely used throughout the world for interferon based therapy prediction for HCV patients and is quite helpful not only for health care workers but also for the patients. There is a strong relationship between single nucleotide polymorphisms at or near the IL-28 B gene and the sustained virological response with pegylated interferon plus ribavirin treatment for chronic hepatitis C. Pakistan is a resource limited country, with very low per capita income and there is no proper social security(health insurance) system. The allocated health budget by the government is very low and is used on other health emergencies like polio virus and dengue virus infection. Therefore it is proposed that there should be a well established diagnostic test on the basis of IL-28 B which can predict the antiviral therapy response to strengthen health care set-up of Pakistan. This test once established will help in better management of HCV infected patients. 展开更多
关键词 Diagnostics HEPATITIS c virus INTERFERON THERAPY POLYMORPHISMS IL-28B Genetic testing Pakistan
下载PDF
体外膜氧合联合介入取栓救治蛋白C基因突变所致高危肺栓塞一例
14
作者 谢玉华 张安 +1 位作者 曾瀚庆 曹云星 《中国医学科学院学报》 CAS CSCD 北大核心 2024年第2期293-296,共4页
遗传性蛋白C缺陷症是由蛋白C基因突变引起的一种染色体遗传病,可导致静脉血栓形成,多与外显子4~9和内含子8的突变有关。蛋白C基因突变引起的致死性肺栓塞罕见,治疗面临巨大挑战。本文报道1例由蛋白C基因8号外显子移码突变引起的致死性... 遗传性蛋白C缺陷症是由蛋白C基因突变引起的一种染色体遗传病,可导致静脉血栓形成,多与外显子4~9和内含子8的突变有关。蛋白C基因突变引起的致死性肺栓塞罕见,治疗面临巨大挑战。本文报道1例由蛋白C基因8号外显子移码突变引起的致死性肺栓塞,采用体外膜氧合进行呼吸、循环支持,并成功实行介入取栓的救治经验,为该疾病的诊断及救治提供参考。 展开更多
关键词 蛋白c缺陷症 蛋白c基因 基因检测 肺栓塞 体外膜氧合 介入取栓
下载PDF
Ni/c-BN对激光熔覆316L不锈钢熔覆层组织与耐蚀性的影响
15
作者 刘亚迪 杜学山 +2 位作者 史考 王志浩 孙玉福 《铸造》 CAS 2024年第3期343-348,共6页
采用激光熔覆技术在马氏体不锈钢(ZG06Cr13Ni4Mo)表面制备了Ni/c-BN增强316L不锈钢复合熔覆层。通过XRD、SEM、EDS、电化学试验和冲蚀试验分析了不同Ni/c-BN含量对熔覆层的显微组织和和耐蚀性影响。结果表明,复合熔覆层的物相由FeCr_(0.... 采用激光熔覆技术在马氏体不锈钢(ZG06Cr13Ni4Mo)表面制备了Ni/c-BN增强316L不锈钢复合熔覆层。通过XRD、SEM、EDS、电化学试验和冲蚀试验分析了不同Ni/c-BN含量对熔覆层的显微组织和和耐蚀性影响。结果表明,复合熔覆层的物相由FeCr_(0.29)Ni_(0.16)C_(0.06)固溶体组成,随着Ni/c-BN添加量的增加,其顶部组织的等轴晶数量减少,胞状树枝晶的数量增多。复合熔覆层的耐蚀性随Ni/c-BN添加量的增加呈先升高后降低的趋势,当Ni/c-BN添加量为5wt.%时,熔覆层的耐蚀性最好。过量的Ni/c-BN使得熔覆层表面存在气孔,且导致熔覆层内部存在少量大尺寸残留c-BN,降低了熔覆层的耐腐蚀性和耐冲蚀性。 展开更多
关键词 激光熔覆 c-BN 316L不锈钢熔覆层 电化学试验 冲蚀试验
下载PDF
High Heat Flux Testing of B_4C/Cu and SiC/Cu Functionally Graded Materials Simulated by Laser and Electron Beam 被引量:4
16
作者 刘翔 谌继明 +3 位作者 张斧 许增裕 葛昌纯 李江涛 《Plasma Science and Technology》 SCIE EI CAS CSCD 2002年第1期1171-1176,共6页
B4C, SiC and C, Cu functionally graded-materials (FGMs) have been developed by plasma spraying and hot pressing. Their high-heat flux properties have been investigated by high energy laser and electron beam for the si... B4C, SiC and C, Cu functionally graded-materials (FGMs) have been developed by plasma spraying and hot pressing. Their high-heat flux properties have been investigated by high energy laser and electron beam for the simulation of plasma disruption process of the future fusion reactors, And a study on eroded products of B4C/Cu FGM under transient thermal load of electron beam was performed. In the experiment, SEM and EDS analysis indicated that B4C and SiC were decomposed, carbon was preferentially evaporated under high thermal load, and a part of Si and Cu were melted, in addition, the splash of melted metal and the particle emission of brittle destruction were also found. Different erosive behaviors of carbon-based materials (CBMs) caused by laser and electron beam were also discussed. 展开更多
关键词 SIc High Heat Flux testing of B4c/cu and Sic/cu Functionally Graded Materials Simulated by Laser and Electron Beam cU
下载PDF
基于Cypress的医疗Web平台自动化测试设计与实现
17
作者 翟承珏 于泓涛 梁振 《中国医疗设备》 2024年第3期14-19,共6页
目的 把自动化测试应用于医疗Web平台(以下简称医疗平台),以提高医疗平台测试的效率并保证平台的稳定性。方法 分析Cypress原理及流程,搭建测试框架,并编写测试用例,再结合Cypress自动化测试框架编写测试代码,将自动化测试覆盖医疗平台... 目的 把自动化测试应用于医疗Web平台(以下简称医疗平台),以提高医疗平台测试的效率并保证平台的稳定性。方法 分析Cypress原理及流程,搭建测试框架,并编写测试用例,再结合Cypress自动化测试框架编写测试代码,将自动化测试覆盖医疗平台的各个关键功能模块。结果 根据实际测试验证分析,随着回归测试量增多(回归测试大于3时),自动化测试相较于手动测试,能够显著提高测试效率。使用两项系统功能进行多次手动和自动化对比测试,结果显示,自动化测试速度明显快于手动测试,差异具有统计学意义(P<0.001)。结论 基于Cypress框架的医疗平台自动化测试是一种有效的测试方案,可提高医疗平台的测试效率,为医疗平台开发测试提供了可靠的技术参考。 展开更多
关键词 cYPRESS 自动化测试 医疗Web平台 测试框架
下载PDF
Expi293F表达重组鲎C因子用于荧光法内毒素检测
18
作者 柯文锋 李诗洁 +2 位作者 郑梦林 白仲虎 杨艳坤 《食品与发酵工业》 CAS CSCD 北大核心 2024年第2期106-111,共6页
鲎C因子是一种对痕量内毒素高度敏感的丝氨酸蛋白酶原,在医药行业可替代鲎试剂用于内毒素检测。然而其重组表达困难,且对于环境中内毒素的控制要求极高。该文首先克隆了来源于东方鲎的全长C因子,并在N末端和C末端分别添加了Flag标签和6&... 鲎C因子是一种对痕量内毒素高度敏感的丝氨酸蛋白酶原,在医药行业可替代鲎试剂用于内毒素检测。然而其重组表达困难,且对于环境中内毒素的控制要求极高。该文首先克隆了来源于东方鲎的全长C因子,并在N末端和C末端分别添加了Flag标签和6×His标签,同时用哺乳细胞表达载体中的分泌信号肽替换掉了原始C因子信号肽。构建好的重组质粒转染至人胚胎肾脏细胞Expi293F悬浮培养7 d后收获上清液,Western Blot检测蛋白与预测大小128 kDa一致,具有与内毒素结合以及结合后切割荧光底物的能力。该研究首次在人胚胎肾脏细胞Expi293F中成功表达出具有生物学活性的全长C因子,表达量达到19.18 mg/L,比DING在昆虫细胞SF9中的表达量提高了113%。该方法对于后续开发低成本内毒素检测试剂盒具有指导意义。 展开更多
关键词 内毒素检测 c因子 Expi293F 重组表达 脂质体转染
下载PDF
三自由度精密检测平台结构设计及Workbench响应面优化
19
作者 李梦涛 吴国新 +1 位作者 王爱军 何学军 《工具技术》 北大核心 2024年第2期136-140,共5页
孔径测量仪是测量环规直径或量块端度的常用仪器,三自由度精密检测平台是孔径测量仪机械部分的核心,可以稳定支撑待测物,并能进行手动独立调节,满足孔径测量要求。对三自由度精密检测平台进行结构设计,使其满足y向平移、M_(z)偏转、M_(y... 孔径测量仪是测量环规直径或量块端度的常用仪器,三自由度精密检测平台是孔径测量仪机械部分的核心,可以稳定支撑待测物,并能进行手动独立调节,满足孔径测量要求。对三自由度精密检测平台进行结构设计,使其满足y向平移、M_(z)偏转、M_(y)倾斜三个自由度的调节。从SolidWorks建立的三自由度精密检测平台装配体中取出底层板三维模型,化简后导入ANSYS Workbench中进行静力学分析,得到其变形云图和应力云图。为降低平台的变形量,在ANSYS中对底层版进行响应面优化分析。响应面优化分析选用中心复合实验设计,设计变量为底板厚度和三个弹簧立柱的支撑力,响应为变形量,得到25组实验点。根据实验结果,利用Kriging插值法建立了底板的响应面模型,并对各相关设计变量进行了敏感度分析。使用多目标遗传算法完成了底板的优化设计,与原始设计的结构方案相比,平台的变形量减少20.1%。 展开更多
关键词 检测平台 结构设计 响应面优化 SOLIDWORKS ANSYS
下载PDF
C-V2X车辆恶意数据识别能力测试方法
20
作者 王华森 秦孔建 +1 位作者 马文博 张美芳 《无线电工程》 2024年第8期1881-1889,共9页
针对测试车载单元(On Board Unit, OBU)或整车是否具备识别恶意数据的能力以及其恶意数据识别能力是否符合要求的问题,提出了一种恶意数据识别能力测试方法,搭建了蜂窝车联网(Cellular-Vehicle to Everything, C-V2X)信息安全测试系统... 针对测试车载单元(On Board Unit, OBU)或整车是否具备识别恶意数据的能力以及其恶意数据识别能力是否符合要求的问题,提出了一种恶意数据识别能力测试方法,搭建了蜂窝车联网(Cellular-Vehicle to Everything, C-V2X)信息安全测试系统。根据恶意数据消息的发送主体的合法性、一致性和安全层校验通过情况,将常见恶意数据攻击进行分类。基于分类结果,设计相应测试用例,利用测试设备生成非法安全协议数据单元(Session Primitive Data Unit, SPDU)数据包。C-V2X信息安全测试中包括车用无线通信技术(Vehicle to Everything, V2X)模拟模块、全球导航卫星系统(Global Navigation Satellite System, GNSS)模拟模块、证书授权(Certificate Authority, CA)平台和场景模拟模块等几个部分,在C-V2X模拟模块采用FPGA处理上位机的比特流,并采用数字上变频(Digital Up Converters, DUC)、数字下变频(Digital Down Converter, DDC)和数模转换器(Digital to Analog Converter, DAC)等几个模块完成数据处理。结果表明,所提测试方法可以模拟常见的恶意数据攻击,测试设备生成的合法数据包可以被车辆正常接受,生成的非法数据包部分可以被待测设备识别并丢弃。 展开更多
关键词 车用无线通信技术 恶意数据 信息安全测试 测试平台
下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部