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Construction Cost Control Strategy of Finished House Based on BIM-5D
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作者 Dongchun Xia Xinyue Zhou 《Journal of World Architecture》 2024年第4期15-20,共6页
Building Information Modeling(BIM)technology can not only achieve project refinement management of the construction process based on a 3D model but also form a BIM-5D model based on the fusion of progress data and cos... Building Information Modeling(BIM)technology can not only achieve project refinement management of the construction process based on a 3D model but also form a BIM-5D model based on the fusion of progress data and cost data to achieve the dynamic control of the whole process of project cost.This paper discusses the application of BIM-5D in the cost control of finished house construction,aiming to provide technical reference for China’s finished house project units,so that they can master how to realize the construction of cost control system based on BIM,the monitoring of the key coefficients of the cost,corrective action,to ensure that the project cost deviation is within the controllable range,and to make an effective guarantee for the economic benefits of the project construction. 展开更多
关键词 BIM-5d Finished house project Cost control system Cost corrective deviation
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Scheduling and control co-design for Networked Wind Energy Conversion Systems 被引量:3
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作者 Zhihong Huo Zhixue Zhang 《Global Energy Interconnection》 2019年第4期328-335,共8页
Fieldbus, industrial Ethernet that is simple, reliable, economical, and practical, is widely used in Wind Energy Conversion Systems(WECSs). These techniques belong to the field of networked control systems. Network em... Fieldbus, industrial Ethernet that is simple, reliable, economical, and practical, is widely used in Wind Energy Conversion Systems(WECSs). These techniques belong to the field of networked control systems. Network embedding to Wind Energy Conversion Systems brings many new challenges. Implementing a control system over a communication network causes inevitable time delays that may degrade performance and can even cause instability. This work addresses challenges related to the reliable control of wind energy conversion systems, based on the theoretical framework of networked control systems. A type of WECS with network-induced delay and packet dropout is modeled and adjustable deadbands are explored as a solution to reduce network traffic in WECSs. A method to study the reliable control of WECSs is presented, which takes into account system response as well as the network environment. After detailed theoretical analysis, simulation results are provided, which further demonstrate the feasibility of the proposed scheme. 展开更多
关键词 Wind Energy conversion System(WECS) NETWORKEd control System(NCS) TIME-dELAY deadband Package-dropout
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PMSG Wind Energy Conversion System: Modeling and Control 被引量:1
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作者 Omessaad Elbeji Mouna Ben Hamed Lassaad Sbita 《International Journal of Modern Nonlinear Theory and Application》 2014年第3期88-97,共10页
In this paper, a model of a variable speed wind turbine using a permanent magnet synchronous generator (PMSG) is presented and the control schemes are proposed. The model presents the aerodynamic part of the wind turb... In this paper, a model of a variable speed wind turbine using a permanent magnet synchronous generator (PMSG) is presented and the control schemes are proposed. The model presents the aerodynamic part of the wind turbine, the mechanic and the electric parts. Simulations have been conducted with Matlab/Simulink to validate the model and the proposed control schemes. 展开更多
关键词 WINd Turbine WINd Energy conversion System PERMANENT MAGNET Synchronous Generator Speed control Current control
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Shape Control,Crystalline Conversion and Pseudocapacitance Properties of Mn3O4:Effects of Yb3+Doping
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作者 尤俊华 郭耀祖 +2 位作者 赵耀 倪志远 郭瑞 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2018年第9期1440-1445,共6页
We report a facile method for the synthesis of manganese oxide(Mn3O4) nanorods via the direct reaction of MnCl2 and H2 O2 by doping Yb3+ ions at room temperature and air atmosphere. The Mn3O4:Yb3+ samples were ch... We report a facile method for the synthesis of manganese oxide(Mn3O4) nanorods via the direct reaction of MnCl2 and H2 O2 by doping Yb3+ ions at room temperature and air atmosphere. The Mn3O4:Yb3+ samples were characterized by X-ray diffraction(XRD), scanning electron microscopy(SEM), cyclic voltammetry(CVs), electrochemical impedance spectroscopy(EIS), and charging-discharging test(CD). The results show that trace Yb3+ doping(6 at%) could effectively induce crystalline transformation of Mn3O4 from cubic system(space group Fd-3 m) to tetragonal system(space group I41/amd) and incite the morphology changing from irregular particles to uniform nanorods. When Yb3+ doping amount is 3%, the capacitance of Mn3O4 reaches the maximum, 246 F/g, which is related to the morphology change and the corresponding decrease of impedance. 展开更多
关键词 MN3O4 Yb^3+ shape control crystalline conversion PSEUdOCAPACITOR
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OUTPUT MAXIMIZATION CONTROL FOR VSCF WIND ENERGY CONVERSION SYSTEM USING EXTREMUM CONTROL STRATEGY
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作者 付大丰 马运东 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2013年第2期185-192,共8页
The energy conversion optimization control strategy is presented for a family of horizontal-axis variablespeed fixed-pitch wind energy conversion systems,working in the partial load region.The system uses a variablesp... The energy conversion optimization control strategy is presented for a family of horizontal-axis variablespeed fixed-pitch wind energy conversion systems,working in the partial load region.The system uses a variablespeed wind turbine(VSWT)driving a squirrel-cage induction generator(SCIG)connected to a grid.A new maximum power point tracking(MPPT)approach is proposed based on the extremum seeking control principles under the assumption that the wind turbine model and its parameters are poorly known.The aim is to drive the average position of the operation point close to optimality.Here the wind turbulence is used as search disturbance instead of inducing new sinusoidal search signals.The discrete Fourier transform(DFT)process of some available measures estimates the distance of operation point to optimality.The effectiveness of the proposed MPPT approach is validated under different operation conditions by numerical simulations in MATLAB/SIMULINK.The simulation results prove that the new approach can effectively suppress the vibration of system and enhance the dynamic performance of system. 展开更多
关键词 wind energy conversion systems maximum power point tracking extremum control strategy discrete Fourier transform
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An Improved Torque Sensorless Speed Control Method for Electric Assisted Bicycle With Consideration of Coordinate Conversion
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作者 Tinghua Li Qinghua Yang +1 位作者 Xiaowei Tu Bin Ren 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2020年第6期1575-1584,共10页
In this paper,we propose an improved torque sensorless speed control method for electric assisted bicycle,this method considers the coordinate conversion.A low-pass filter is designed in disturbance observer to estima... In this paper,we propose an improved torque sensorless speed control method for electric assisted bicycle,this method considers the coordinate conversion.A low-pass filter is designed in disturbance observer to estimate and compensate the variable disturbance during cycling.A DC motor provides assisted power driving,the assistance method is based on the realtime wheel angular velocity and coordinate system transformation.The effect of observer is proved,and the proposed method guarantees stability under disturbances.It is also compared to the existing methods and their performances are illustrated through simulations.The proposed method improves the performance both in rapidity and stability. 展开更多
关键词 Coordinate conversion disturbance observer electric assisted power inertial measurement unit speed control
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Method to Increase Micro-controller A/D Conversion Speed
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作者 唐鹤权 《Journal of Donghua University(English Edition)》 EI CAS 2004年第5期128-131,共4页
The speed and cost of A/D conversion are conflict to each other. As micro-controller speed is increasing and its internal function is improving, full utilization of its internal resource can dramatically increase A/D ... The speed and cost of A/D conversion are conflict to each other. As micro-controller speed is increasing and its internal function is improving, full utilization of its internal resource can dramatically increase A/D conversion speed and without compromising conversion quality. MSP430F11x1 series micro-controller is exploited to increase A/D conversion speed. 展开更多
关键词 Micro-controller a/d conversion Speed and cost.
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Adaptive Fuzzy Sliding Mode Controller for Grid Interface Ocean Wave Energy Conversion
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作者 Adel A. A. Elgammal 《Journal of Intelligent Learning Systems and Applications》 2014年第2期53-69,共17页
This paper presents a closed-loop vector control structure based on adaptive Fuzzy Logic Sliding Mode Controller (FL-SMC) for a grid-connected Wave Energy Conversion System (WECS) driven Self-Excited Induction Generat... This paper presents a closed-loop vector control structure based on adaptive Fuzzy Logic Sliding Mode Controller (FL-SMC) for a grid-connected Wave Energy Conversion System (WECS) driven Self-Excited Induction Generator (SEIG). The aim of the developed control method is to automatically tune and optimize the scaling factors and the membership functions of the Fuzzy Logic Controllers (FLC) using Multi-Objective Genetic Algorithms (MOGA) and Multi-Objective Particle Swarm Optimization (MOPSO). Two Pulse Width Modulated voltage source PWM converters with a carrier-based Sinusoidal PWM modulation for both Generator- and Grid-side converters have been connected back to back between the generator terminals and utility grid via common DC link. The indirect vector control scheme is implemented to maintain balance between generated power and power supplied to the grid and maintain the terminal voltage of the generator and the DC bus voltage constant for variable rotor speed and load. Simulation study has been carried out using the MATLAB/Simulink environment to verify the robustness of the power electronics converters and the effectiveness of proposed control method under steady state and transient conditions and also machine parameters mismatches. The proposed control scheme has improved the voltage regulation and the transient performance of the wave energy scheme over a wide range of operating conditions. 展开更多
关键词 GRId integration Wave Energy conversion Systems Self-Excited Induction Generator (SEIG) Vector control Genetic Algorithm (GA) Particle SWARM Optimization (PSO) SLIdING Mode control (SMC) Fuzzy Logic control (FLC) MEMBERSHIP Function Tuning
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Event-triggered mechanism based robust fault-tolerant control for networked wind energy conversion system
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作者 Zhihong Huo Chang Xu 《Global Energy Interconnection》 EI CAS CSCD 2022年第1期55-65,共11页
In this paper,a novel robust fault-tolerant control scheme based on event-triggered communication mechanism for a variable-speed wind energy conversion system(WECS)with sensor and actuator failures is proposed.The non... In this paper,a novel robust fault-tolerant control scheme based on event-triggered communication mechanism for a variable-speed wind energy conversion system(WECS)with sensor and actuator failures is proposed.The nonlinear WECS with event-triggered mechanism is modeled based on the Takagi-Sugeno(T-S)fuzzy model.By Lyapunov stability theory,the parameter expression of the proposed robust fault-tolerant controller with event-triggered mechanisms is proposed based on a feasible solution of linear matrix inequalities.Compared with the existing WECS fault-tolerant control methods,the proposed scheme significantly reduces the pressure of network packet transmission and improves the robustness and reliability of the WECS.Considering a doubly-fed variable speed constant frequency wind turbine,the eventtriggered mechanism based fault-tolerant control for WECS is analyzed considering system model uncertainty.Numerical simulation results demonstrate that the proposed scheme is feasible and effective. 展开更多
关键词 Event-triggered mechanism(ETM) Wind energy conversion system(WECS) Fault-tolerant control(FTC) Takagi-sugeno(T-S)fuzzy model
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Hybrid Control Strategy for Matrix Converter Fed Wind Energy Conversion System
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作者 Jamna Ayadathil Jamuna Venkatesan 《Circuits and Systems》 2016年第10期3038-3053,共16页
In this paper, a hybrid control strategy for a matrix converter fed wind energy conversion system is presented. Since the wind speed may vary, output parameters like power, frequency and voltage may fluctuate. Hence i... In this paper, a hybrid control strategy for a matrix converter fed wind energy conversion system is presented. Since the wind speed may vary, output parameters like power, frequency and voltage may fluctuate. Hence it is necessary to design a system that regulates output parameters, such as voltage and frequency, and thereby provides a constant voltage and frequency output from the wind energy conversion system. Matrix converter is used in the proposed solution as the main power conditioner as a more efficient alternative when compared to traditional back-back converter structure. To control the output voltage, a vector modulation based refined control structure is used. A power tracker is included to maximize the mechanical output power of the turbine. Over current protection and clamp circuit input protection have been introduced to protect the system from over current. It reduces the spikes generated at the output of the converter. The designed system is capable of supplying an output voltage of constant frequency and amplitude within the expected ranges of input during the operation. The matrix converter control using direct modulation method, modified Venturini modulation method and vector modulation method was simulated, the results were compared and it was inferred that vector modulation method was superior to the other two methods. With the proposed technique, voltage transfer ratio and harmonic profile have been improved compared to the other two modulation techniques. The behaviour of the system is corroborated by MATLAB Simulink, and hardware is realized using an FPGA controller. Experimental results are found to be matching with the simulation results. 展开更多
关键词 Hybrid control Strategy Wind Energy conversion System Three Phase Matrix Converter Space Vector Modulation Over Current/Clamp Circuit Protection FPGA controller
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基于RFID的医疗设备远程管理系统设计 被引量:3
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作者 向明华 赵菊云 +1 位作者 向国华 吴玲 《自动化技术与应用》 2024年第3期62-65,135,共5页
为提高医院内设备定位精度,设计基于RFID的医疗设备远程管理系统。通过阅读器、电子标签和处理器组成RFID节点,引入AS3992芯片作为核心芯片,实现数据处理和编码,执行远程指令。采用自由空间传播模型计算路径损耗产生值,通过三角定位数... 为提高医院内设备定位精度,设计基于RFID的医疗设备远程管理系统。通过阅读器、电子标签和处理器组成RFID节点,引入AS3992芯片作为核心芯片,实现数据处理和编码,执行远程指令。采用自由空间传播模型计算路径损耗产生值,通过三角定位数学方法计算待定标签位置,采用RFID定位方法确定标签的最近邻居,实现医疗设备的远程定位。实验结果表明,在没有障碍物的情况下,所提系统设备定位误差少于1 m,在有障碍物的情况下,定位误差在2 m左右,证明所提出系统定位准确性较高,满足系统的设计需求。 展开更多
关键词 RFId 医疗设备远程管理 路径损耗 a/d转换
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基于D-分割法的直流变换器遗传自抗扰控制器设计
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作者 周雪松 王鑫 +3 位作者 马幼捷 王博 赵明 问虎龙 《太阳能学报》 EI CAS CSCD 北大核心 2024年第9期378-385,共8页
针对光伏发电系统中DC-DC变换器由于负载和工作环境条件变化等扰动引起的输出波动问题,提出一种基于D-分割法的直流变换器遗传自抗扰控制器(ADRC)设计方法。该方法适用于设计光伏发电领域中的双向DC-DC变换器,采用D-分割法获得满足闭环... 针对光伏发电系统中DC-DC变换器由于负载和工作环境条件变化等扰动引起的输出波动问题,提出一种基于D-分割法的直流变换器遗传自抗扰控制器(ADRC)设计方法。该方法适用于设计光伏发电领域中的双向DC-DC变换器,采用D-分割法获得满足闭环系统鲁棒稳定的ADRC控制器参数范围;利用具有全局寻优能力的遗传算法,按综合性能指标在该范围内进行参数寻优。实验结果表明,所提基于D-分割法的直流变换器遗传自抗扰控制器设计方法能有效抑制微网母线侧的电压波动和负载突变,提高控制器的鲁棒性,增强光伏发电系统的动态响应性能和抗干扰能力。 展开更多
关键词 光伏发电 dC-dC变换器 遗传算法 自抗扰控制 d-分割法
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基于单片机开发板和3D打印的一种多参数呼吸训练装置的研究开发
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作者 赵东升 张龙 +2 位作者 孔康辉 郭鸣周 娄阳 《中国医疗设备》 2024年第6期17-22,29,共7页
目的通过野火骄阳32单片机开发板和3D建模及打印技术进行软硬件开发,实现一种多参数呼吸训练装置的专利样机转化。方法利用野火骄阳32单片机开发板开发应用程序,通过单片机开发板实现流量信号和压力信号的输入,舵机阀、涡轮风机、电磁... 目的通过野火骄阳32单片机开发板和3D建模及打印技术进行软硬件开发,实现一种多参数呼吸训练装置的专利样机转化。方法利用野火骄阳32单片机开发板开发应用程序,通过单片机开发板实现流量信号和压力信号的输入,舵机阀、涡轮风机、电磁阀的控制输出与上位机的数据通信等功能;样机非标零部件通过3D建模及打印技术完成制造,并将单片机开发板、标准零部件和非标零部件进行组合以完成样机开发。结果野火骄阳32单片机开发实现了传感器数据采集、运动部件的控制输出、上位机软件的数据上传和接收;3D建模及打印技术实现了非标零部件及机器外壳的制作,以低成本成功制作出样机;样机通过质控仪检测,流量值误差范围为±15%,气道压力值误差范围为±[2%FS(满量程)+4%×实际读数],样机参数精度满足JJF1234-2018《呼吸机校准规范》要求。结论野火骄阳32单片机开发板和3D建模及打印技术的应用,以低成本实现了样机制作,能够为专利的商业转化提供重要参考,为医工专利转化提供了一种新的思路。 展开更多
关键词 单片机开发板 3d建模及打印技术 专利转化 控制系统 压力传感器 流量传感器
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一种双控制模式的同步降压型DC/DC转换器
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作者 李新 佟宇航 高从勇 《微处理机》 2024年第4期12-16,共5页
为突破转换器在输出电容选型上的局限性,保证转换器快速的负载瞬态响应,基于自适应恒定导通时间控制的两种控制模式,提出一种可根据输出电容类型选择控制模式的同步降压型DC/DC转换器。在D-CAP模式下,支持具有适当等效串联电阻的电容等... 为突破转换器在输出电容选型上的局限性,保证转换器快速的负载瞬态响应,基于自适应恒定导通时间控制的两种控制模式,提出一种可根据输出电容类型选择控制模式的同步降压型DC/DC转换器。在D-CAP模式下,支持具有适当等效串联电阻的电容等作为输出电容;在电流模式下,支持等效串联电阻较低的陶瓷电容作为输出电容。基于0.35μm BCD工艺,使用Cadence软件对转换器进行瞬态仿真。结果表明,该设计在两种模式下都具有快速的瞬态响应。 展开更多
关键词 自适应恒定导通时间控制 直接电容感应控制 快速瞬态响应
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Applications of carbonic acid solution for developing conversion coatings on Mg alloy 被引量:6
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作者 余秉隆 林俊凱 汪俊延 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2010年第7期1331-1339,共9页
Works on exploring an environmentally clean method for producing an Mg,Al-hydrotalcite(Mg6Al2(OH) 16CO3·4H2O) layer and/or calcium carbonate(CaCO3) layer on Mg alloy in a carbonic acid solution system(aqueous HCO... Works on exploring an environmentally clean method for producing an Mg,Al-hydrotalcite(Mg6Al2(OH) 16CO3·4H2O) layer and/or calcium carbonate(CaCO3) layer on Mg alloy in a carbonic acid solution system(aqueous HCO3-/CO3 2-or Ca 2+ /HCO3-) at 50℃ were reviewed.Conversion treatment for the Mg,Al-hydrotalcite conversion coating was as follows.Mg alloy was treated first in acidic HCO3-/CO3 2-aqueous for precursor layer formation on Mg alloy surface and then in alkaline HCO3-/CO3 2-aqueous to form a crystallized Mg,Al-hydrotalcite coating.Duration of an Mg,Al-hydrotalcite coating on Mg alloy surface was reduced from 12 h to 4 h by the conversion treatment.On the other hand,for reducing the formation time of CaCO3 coating on Mg alloy,the aqueous Ca 2+ /HCO3-with a saturated Ca 2+ content was employed for developing a CaCO3 coating on Mg alloy.A dense CaCO3 coating could yield on Mg alloy surface in 2 h.Corrosion rate(corrosion current density,Jcorr) of the Mg,Al-hydrotalcite-coated sample and CaCO3-coated AZ91D sample was 7-10μA/cm 2,roughly two orders less than the Jcorr of the as-diecast sample(about 200μA/cm 2) . No corrosion spot on the Mg,Al-hydrotalcite-coated sample and CaCO3-coated sample was observed after 72 h and 192 h salt spray test,respectively. 展开更多
关键词 Mg alloy AZ91d alloy CORROSION conversion coating carbonic acid
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Controlled Synthesis of Graphdiyne-Based Multiscale Catalysts for Energy Conversion 被引量:1
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作者 Siao Chen Xuchen Zheng +3 位作者 Yang Gao Xinyu Ping Yurui Xue Yuliang Li 《Precision Chemistry》 2024年第7期355-375,共21页
Graphdiyne(GDY)science is a new and rapidly developing interdisciplinary field that touches on various areas of chemistry,physics,information science,material science,life science,environmental science,and so on.The r... Graphdiyne(GDY)science is a new and rapidly developing interdisciplinary field that touches on various areas of chemistry,physics,information science,material science,life science,environmental science,and so on.The rapid development of GDY science is part of the trend in development of carbon materials.GDY,with its unique structure and fascinating properties,has greatly promoted fundamental research toward practical applications of carbon materials.Many important applications,such as catalysis and energy conversion,have been reported.In particular,GDY has shown great potential for application in the field of catalysis.Scientists have precisely synthesized a series of GDY-based multiscale catalysts and applied them in various energy conversion and catalysis research,including ammonia synthesis,hydrogen production,CO_(2) conversion,and chemical-to-electrical energy conversion.In this paper,we systematically review the advances in the precisely controlled synthesis of GDY and aggregated structures,and the latest progress with GDY in catalysis and energy conversion. 展开更多
关键词 graphdiyne controlled growth atom catalyst HETEROSTRUCTURE energy conversion
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A transceiver frequency conversion module based on 3D micropackaging technology 被引量:4
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作者 LIU Boyuan WANG Qingping +1 位作者 WU Weiwei YUAN Naichang 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2020年第5期899-907,共9页
The idea of Ku-band transceiver frequency conversion module design based on 3D micropackaging technology is proposed. By using the double frequency conversion technology,the dual transceiver circuit from Ku-band to L-... The idea of Ku-band transceiver frequency conversion module design based on 3D micropackaging technology is proposed. By using the double frequency conversion technology,the dual transceiver circuit from Ku-band to L-band is realized by combining with the local oscillator and the power control circuit to complete functions such as amplification, filtering and gain. In order to achieve the performance optimization and a high level of integration of the Ku-band monolithic microwave integrated circuits(MMIC) operating chip, the 3 D vertical interconnection micro-assembly technology is used. By stacking solder balls on the printed circuit board(PCB), the technology decreases the volume of the original transceiver to a miniaturized module. The module has a good electromagnetic compatibility through special structure designs. This module has the characteristics of miniaturization, low power consumption and high density, which is suitable for popularization in practical application. 展开更多
关键词 KU-BANd frequency conversion 3d packaging CHIP electromagnetic compatibility
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An Adaptive Wireless Power Sharing Control for Multiterminal HVDC
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作者 Hasan Alrajhi 《Computer Systems Science & Engineering》 SCIE EI 2023年第4期117-129,共13页
Power sharing among multiterminal high voltage direct current terminals(MT-HVDC)is mainly developed based on a priority or sequential manners,which uses to prevent the problem of overloading due to a predefined contro... Power sharing among multiterminal high voltage direct current terminals(MT-HVDC)is mainly developed based on a priority or sequential manners,which uses to prevent the problem of overloading due to a predefined controller coefficient.Furthermore,fixed power sharing control also suffers from an inability to identify power availability at a rectification station.There is a need for a controller that ensures an efficient power sharing among the MT-HVDC terminals,prevents the possibility of overloading,and utilizes the available power sharing.A new adaptive wireless control for active power sharing among multiterminal(MT-HVDC)systems,including power availability and power management policy,is proposed in this paper.The proposed control strategy solves these issues and,this proposed controller strategy is a generic method that can be applied for unlimited number of converter stations.The rational of this proposed controller is to increase the system reliability by avoiding the necessity of fast communication links.The test system in this paper consists of four converter stations based on three phase-two AC voltage levels.The proposed control strategy for a multiterminal HVDC system is conducted in the power systems computer aided design/electromagnetic transient design and control(PSCAD/EMTDC)simulation environment.The simulation results significantly show the flexibility and usefulness of the proposed power sharing control provided by the new adaptive wireless method. 展开更多
关键词 Active power control fixed power sharing current control HVdC transmission MTdC voltage source converter(VSC) power sharing control adaptive wireless control power conversion
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Transcription factor-based gene therapy to treat glioblastoma through direct neuronal conversion 被引量:3
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作者 Xin Wang Zifei Pei +2 位作者 Aasma Hossain Yuting Bai Gong Chen 《Cancer Biology & Medicine》 SCIE CAS CSCD 2021年第3期860-874,共15页
Objective:Glioblastoma(GBM)is the most prevalent and aggressive adult primary cancer in the central nervous system.Therapeutic approaches for GBM treatment are under intense investigation,including the use of emerging... Objective:Glioblastoma(GBM)is the most prevalent and aggressive adult primary cancer in the central nervous system.Therapeutic approaches for GBM treatment are under intense investigation,including the use of emerging immunotherapies.Here,we propose an alternative approach to treat GBM through reprogramming proliferative GBM cells into non-proliferative neurons.Methods:Retroviruses were used to target highly proliferative human GBM cells through overexpression of neural transcription factors.Immunostaining,electrophysiological recording,and bulk RNA-seq were performed to investigate the mechanisms underlying the neuronal conversion of human GBM cells.An in vivo intracranial xenograft mouse model was used to examine the neuronal conversion of human GBM cells.Results:We report efficient neuronal conversion from human GBM cells by overexpressing single neural transcription factor Neurogenic differentiation 1(Neuro D1),Neurogenin-2(Neurog2),or Achaete-scute homolog 1(Ascl1).Subtype characterization showed that the majority of Neurog2-and Neuro D1-converted neurons were glutamatergic,while Ascl1 favored GABAergic neuron generation.The GBM cell-converted neurons not only showed pan-neuronal markers but also exhibited neuron-specific electrophysiological activities.Transcriptome analyses revealed that neuronal genes were activated in glioma cells after overexpression of neural transcription factors,and different signaling pathways were activated by different neural transcription factors.Importantly,the neuronal conversion of GBM cells was accompanied by significant inhibition of GBM cell proliferation in both in vitro and in vivo models.Conclusions:These results suggest that GBM cells can be reprogrammed into different subtypes of neurons,leading to a potential alternative approach to treat brain tumors using in vivo cell conversion technology. 展开更多
关键词 GLIOBLASTOMA neuronal conversion transcription factors Neuro d1 neurogenin-2 Ascl1
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Calcium phosphate conversion technique:A versatile route to develop corrosion resistant hydroxyapatite coating over Mg/Mg alloys based implants 被引量:2
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作者 G.S.Hikku C.Arthi +2 位作者 R.B.Jeen Robert K.Jeyasubramanian R.Murugesan 《Journal of Magnesium and Alloys》 SCIE EI CAS CSCD 2022年第7期1821-1845,共25页
Globally,vast research interest is emerging towards the development of biodegradable orthopedic implants as it overcomes the toxicity exerted by non-degradable implants when fixed in the human body for a longer period... Globally,vast research interest is emerging towards the development of biodegradable orthopedic implants as it overcomes the toxicity exerted by non-degradable implants when fixed in the human body for a longer period.In this context,magnesium(Mg)plays a major role in the production of biodegradable implants owing to their characteristic degradation nature under the influence of body fluids.Also,Mg is one of the essential nutrients required to perform various metabolic activities by the human cells,and therefore,the degraded Mg products will be readily absorbed by the nearby tissues.Nevertheless,the higher corrosion rate in the biological environment is the primary downside of using Mg implants that liberate H2gas resulting in the formation of cavities.Further,in certain cases,Mg undergoes complete degradation before the healing of damaged bone tissue and cannot serve the purpose of providing mechanical support.So,many studies have been focused on the development of different strategies to improve the corrosion-resistant behavior of Mg according to the requirement.In this regard,the present review focused on the limitations of using pure Mg and Mg alloys for the fabrication of medical implants and how the calcium phosphate conversion coating alters the corrosive tendency through the formation of hydroxyapatite protective films for enhanced performance in medical implant applications. 展开更多
关键词 Biodegradable implants MAGNESIUM controlled corrosion ALLOYING HYdROXYAPATITE Calcium phosphate conversion coating
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