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Structural Characterization of Chloroplast Genome in Alpinia japonica(Thunb.)Miq.,a Medicinal Plant of the Genus Alpinia
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作者 Wentao Sheng Xi Lei +1 位作者 Xinjie Chen Quan Kuang 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第8期1897-1911,共15页
The analysis of chloroplast gene characteristics in Alpinia japonica(Thunb.)Miq.is of great significance for developing relevant genetic resources.The high-throughput sequencing and bioinformatic research were perform... The analysis of chloroplast gene characteristics in Alpinia japonica(Thunb.)Miq.is of great significance for developing relevant genetic resources.The high-throughput sequencing and bioinformatic research were performed to analyze the chloroplast genome characteristics of A.japonica.The total chloroplast genome length of A.japonica was 161,906 bp,with a typical circular tetrameric structure.And 133 genes were annotated,comprising 87 protein-coding,38 tRNA,and 8 rRNA genes.Furthermore,22 genes contained two copies,and 18 genes owned introns.Repeat sequence analysis showed that it contains 321 simple sequence repeats(SSRs)and 37 long segment repeats.Compared with the chloroplast genomes of eight representative plants in the genus Alpinia,the gene structure,type,and quantity were relatively conservative.Rps12 was the highest variation site in the entire chloroplast gene.A phylogenetic tree showed that the genus Alpinia was the most closely related to the genus Amomum.Meanwhile,A.japonica is the most closely related to Alpinia chinensis belonging to the genus Alpinia.Overall,the chloroplast genome of a new species was reported in the genus Alpinia,and a basis was provided for the utilization of Alpinia plants as a medical resource. 展开更多
关键词 The genus alpinia alpinia japonica(Thunb.)miq CHLOROPLaST genome phylogenetic tree
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Effects of Different Seed Stem Sizes on the Changes of Available Elements in Rhizosphere Soil of Fritillaria thunbergii Miq.
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作者 Yong PENG Rui PAN +3 位作者 Zhengyan LIU Xiaohong WU Nong ZHOU Wenwu YANG 《Agricultural Biotechnology》 2024年第3期18-23,共6页
[Objectives]This study was conducted to screen suitable seed stems of Fritillaria thunbergii Miq.from different provenances and to provide a theoretical basis for the high-yielding and high-efficiency cultivation of F... [Objectives]This study was conducted to screen suitable seed stems of Fritillaria thunbergii Miq.from different provenances and to provide a theoretical basis for the high-yielding and high-efficiency cultivation of F.thunbergii Miq.introduced to different places.[Methods]F.thunbergii Miq.from four different provenances including Zhejiang,Nantong and Chongqing were introduced and cultivated in Wanzhou of Chongqing.The contents of available Zn,Fe,Mn,Cu,Mo,N,P,K,Ca and Mg in rhizosphere soil of F.thunbergii Miq.during five growing stages were determined after selecting different stem sizes for field cultivation.[Results]Small stems of Pan an and Ningbo provenances(SSG3,121-160/kg)and middle stems of Nantong and Fengjie provenances(SSG2,81-120/kg)showed higher soil availability.[Conclusions]In the process of introduction and cultivation of F.thunbergii Miq.,high yield and high efficiency can be achieved by selecting smaller seed stems of F.thunbergii Miq. 展开更多
关键词 Fritillaria thunbergii miq. Different provenances Stem size INTRODUCTION CULTIVaTION Soil nutrient element aVaILaBILITY
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Constituents from Ranunculus sieboldii Miq. 被引量:7
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作者 潘云雪 周长新 +2 位作者 张水利 郑筱祥 赵昱 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第2期92-96,共5页
Aim To investigate the chemical composition of Ranunculus sieboldii Miq..Methods Repeated column chromatography over silica gel, polyamide and RP-18 followed by gelfiltration on sephadex LH-20 were used to isolate che... Aim To investigate the chemical composition of Ranunculus sieboldii Miq..Methods Repeated column chromatography over silica gel, polyamide and RP-18 followed by gelfiltration on sephadex LH-20 were used to isolate chemical constituents, and their structures wereelucidated by extensive spectroscopic methods (UV, IR, MS, ~1H NMR, ^(13)C NMR) including 2D NMR(COSY, HMQC, HMBC, NOESY) techniques and by direct comparing spectral data with those reported inliterature. Results Five flavonoid glycosides named apigenin-4'-O-α-L-rhamnopyranoside (1),apigenin-7-O-β-D-glucopyranosyl-4'-O-α-L-rhamnopyranoside (2), apigenin-8-C-α-L-arabinopyranoside(3), apigenin-8-C-β-D-ga-lactopyranoside (4) , tricin-7-O-β-D-glucopyranoside (5), together withtricin (6), luteolin (7), scopoletin (8), esculetin (9), scoparone (10), ferulic acid (11),protocatechuic acid (12) , and tematolide (13) were isolated from the 95% etha-nolic extract of itswhole plant, and their cytotoxic activities were preliminarily tested. Conclusion Compounds 1-12were obtained from this genus and compound 13 from this species for the first time. Furthermore,compound 1 was for the first time isolated from nature while the ^(13)C NMR data of compounds 2 and3 are reported for the first time. The bioassay revealed that compound 1 was active against BEL-7407and A549 cell lines (IC_(45) 43, 77 μg·mL^(-1)), 8 and 10 showed inhibitory activities on KB celllines (IC_(50) 78, 44 μg·mL^(-1)) and HL-60 cell lines (Ic_(50) 85, 85 μg·mL^(-1)), while 7exerted moderate cytotoxic activities on KB, BFL-7407, A549 and HL-60 cell lines with their IC_(50)being 51, 55, 44 and 10 μg·mL^(-1) , respectively. 展开更多
关键词 ranunculus sieboldii miq. flavonoid glycosides apigemn-4'-O-α-L-rhamnopyranoside apigenin-8-C-α-L-arabinopyranoside apigenin-7-O-β-D-glucopvranosyl-4'-O-α-L-rhamnopyranoside cytotoxicity
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Study on Germination Rate of Uncaria rhynchophylla (Miq.) Jacks 被引量:1
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作者 刘涛 刘作易 +1 位作者 贺定祥 刘藜 《Agricultural Science & Technology》 CAS 2008年第4期96-98,117,共4页
[Object] The research aimed to find out seed germination conditions and regulations.[Method] Seeds were soaked by clear water and 4 chemical agents of different concentrations(NaHCO3,KHCO3,K2CO3,NaClO)then conducted g... [Object] The research aimed to find out seed germination conditions and regulations.[Method] Seeds were soaked by clear water and 4 chemical agents of different concentrations(NaHCO3,KHCO3,K2CO3,NaClO)then conducted germination test in illumination box.[Result] Seed germination number and absolute germination rate(above 80%)in water treatment group was obviously higher than other treatments.[Conclusion] No dormancy phenomenon in mature embryo and no inhibition in seed coat,so conventional moist treatment can induct seed germination. 展开更多
关键词 Uncaria rhynchophylla(miq.)Jacks SEED GERMINaTION
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竹叶眼子菜(POTAMOGETON MALAIANUS MIQ.)叶面积测定及与栽培密度的关系初探 被引量:9
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作者 蒲云海 黄运平 李伟 《武汉植物学研究》 CSCD 2000年第4期302-308,共7页
通过人为控制种群密度的栽培实验 ,用叶特征度量估测单叶叶面积 ( AL)、用茎特征度量估测整株叶面积 ( AS) ,分析不同栽培密度对叶面积测定的影响。结果表明 :( 1 )叶长×叶宽 ( L× W)与单叶叶面积相关性最大 ;茎长×茎上... 通过人为控制种群密度的栽培实验 ,用叶特征度量估测单叶叶面积 ( AL)、用茎特征度量估测整株叶面积 ( AS) ,分析不同栽培密度对叶面积测定的影响。结果表明 :( 1 )叶长×叶宽 ( L× W)与单叶叶面积相关性最大 ;茎长×茎上叶片数 ( LS× NL)测定整株叶面积较为适宜。 ( 2 )随着栽培密度增大 ,叶片相对变长变窄 ,比叶面积则增加 ,平均整株叶面积相应减小。( 3)不同的栽培密度对叶片形状有明显影响 ,反映在不同密度处理条件下叶片的几何拟合图形不同。 展开更多
关键词 竹叶眼子菜 栽培密度 叶面积测定
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老鸦瓣(Amana edulis)鳞茎营养成分和生物活性物质含量分析 被引量:2
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作者 张艳秋 吴天宇 +5 位作者 田增智 张宏斌 邢桂梅 张惠华 鲁娇娇 屈连伟 《沈阳农业大学学报》 CAS CSCD 北大核心 2023年第5期522-528,共7页
老鸦瓣[Amana edulis(Miq.)Honda]为百合科(Liliaceae)郁金香属(Tulipa)的一种多年生球根花卉,主要分布于江西、浙江、湖北、安徽、江苏、陕西、山东、辽宁等地。去除鳞茎皮后的干燥鳞茎即为中药“光慈姑”,是中国传统的中药材。目前,... 老鸦瓣[Amana edulis(Miq.)Honda]为百合科(Liliaceae)郁金香属(Tulipa)的一种多年生球根花卉,主要分布于江西、浙江、湖北、安徽、江苏、陕西、山东、辽宁等地。去除鳞茎皮后的干燥鳞茎即为中药“光慈姑”,是中国传统的中药材。目前,研究者对老鸦瓣的研究主要集中在分类、生理、组培、栽培及繁殖等方面,对其药用成分的研究较少,尤其对分布在辽宁的老鸦瓣的营养成分和生物活性物质研究未见报道。为了探讨老鸦瓣的药食用开发价值,以分布在辽宁省大连市境内的老鸦瓣为试验材料,分别在萌芽期和盛花期取母鳞茎、在枯萎期和休眠期取更新鳞茎,进行了营养成分和生物活性物质含量测定。结果表明:老鸦瓣鳞茎中的蛋白质、淀粉、多糖、维生素C、钙和粗纤维等营养成分含量丰富。在萌芽期,母鳞茎维生素C含量最高,为488.513μg·g^(-1),显著高于盛花期,极显著高于枯萎期和休眠期;在盛花期,钙和粗纤维含量最高,分别为1333.412μg·g-1和53.930 mg·g^(-1),两种物质含量均极显著高于其他3个时期;在休眠期,更新鳞茎的蛋白质、淀粉和多糖含量最高,分别为191.162,160.581,115.332 mg·g^(-1),其中,蛋白质含量显著高于萌芽期,极显著高于盛花期和枯萎期,淀粉和多糖2种物质含量均极显著高于其他3个时期。此外,鳞茎中还含有氨基酸、还原糖、皂苷、黄酮、鞣质、脂肪、铁、锌及秋水仙碱等成分,其中,秋水仙碱在不同时期的含量为0.067~0.094μg·g^(-1)。综上,老鸦瓣鳞茎含有丰富的营养成分和多种药用成分,具有较高的药食用开发潜力。 展开更多
关键词 老鸦瓣 鳞茎 药用价值 营养成分
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龙牙楤木总皂苷通过调控AMPK/Nrf2信号抑制缺氧/复氧诱导的心肌细胞铁死亡 被引量:1
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作者 周云洁 常红波 王新洲 《中药材》 CAS 北大核心 2023年第12期3091-3096,共6页
目的:探究龙牙楤木总皂苷对AMPK/Nrf2信号通路的影响。方法:将人心肌细胞AC16进行体外培养,分为空白对照组、缺氧/复氧模型组、铁离子螯合剂对照组,铁离子螯合剂组、龙牙楤木总皂苷低(0.02μg/mL)浓度组、龙牙楤木总皂苷中(0.1μg/mL)... 目的:探究龙牙楤木总皂苷对AMPK/Nrf2信号通路的影响。方法:将人心肌细胞AC16进行体外培养,分为空白对照组、缺氧/复氧模型组、铁离子螯合剂对照组,铁离子螯合剂组、龙牙楤木总皂苷低(0.02μg/mL)浓度组、龙牙楤木总皂苷中(0.1μg/mL)浓度组、龙牙楤木总皂苷高(0.5μg/mL)浓度组,丹参滴丸(50μg/mL)阳性对照组,AMPK抑制剂Compound C(5μg/mL)+龙牙楤木总皂苷(0.5μg/mL)组,Nrf2抑制剂ML385(2μg/mL)+龙牙楤木总皂苷(0.5μg/mL)组。MTT法检测细胞存活率;试剂盒检测细胞内Fe^(2+)、ROS水平;免疫荧光法检测脂质过氧化物及GPX4、ACSL4蛋白表达;qRT-PCR检测GPX4、ACSL4 mRNA表达,Western Blot检测GPX4、ACSL4、p-AMPK、Nrf2蛋白表达;结果:与空白对照组比较,模型组细胞存活率、GPX4 mRNA表达显著降低,Fe^(2+)含量、ROS和脂质过氧化水平、ACSL4 mRNA表达显著升高,p-AMPK、Nrf2蛋白表达显著降低(P<0.01)。与模型组比较,铁离子螯合剂组细胞存活率、GPX4 mRNA表达显著升高,Fe^(2+)、ROS、脂质过氧化水平及ACSL4 mRNA表达显著降低(P<0.01);龙牙楤木总皂苷各组和丹参滴丸组细胞存活率及GPX4、p-AMPK、Nrf2蛋白表达显著升高,心肌细胞中Fe^(2+)、ROS、脂质过氧化水平及ACSL4蛋白表达显著降低(P<0.05或P<0.01)。与龙牙楤木总皂苷组比较,Compound C+龙牙楤木总皂苷组和ML385+龙牙楤木总皂苷组细胞存活率显著降低,Fe^(2+)含量显著升高,Nrf2蛋白表达显著降低,Compound C+龙牙楤木总皂苷组ROS和脂质过氧化水平显著升高,p-AMPK蛋白表达显著降低(P<0.05或P<0.01)。结论:龙牙楤木总皂苷可通过激活AMPK/Nrf2信号通路,抑制H/R诱导的心肌细胞铁死亡。 展开更多
关键词 龙牙楤木总皂苷 心肌细胞 铁死亡 aMPK/Nrf2信号通路
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Current Situation of Fritillaria thunbergii Miq. Production in Yinzhou District of Ningbo City 被引量:1
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作者 Shujun ZHOU 《Asian Agricultural Research》 2017年第1期11-13,18,共4页
Yinzhou District of Ningbo City is the home and main production area of Fritillaria thunbergii Miq. Based on the analysis of Fritillaria thunbergii Miq. production in Yinzhou District in 2015,this paper firstly introd... Yinzhou District of Ningbo City is the home and main production area of Fritillaria thunbergii Miq. Based on the analysis of Fritillaria thunbergii Miq. production in Yinzhou District in 2015,this paper firstly introduced current situation of Fritillaria thunbergii Miq. planting. The competition of Fritillaria thunbergii Miq. products was in disorder,the market price in 2015 dropped to half in 2014,and many Fritillaria thunbergii Miq. growers suffered losses. To keep sustainable development of traditional Fritillaria thunbergii Miq. industry in old production areas and maintain brand and name of genuine materials of " Zhejiang 8 Famous Kinds Herbal Medicines",it is required to actively reduce the inventory,make up short board,strengthen advantages,implement the reform of supply font,keep up with the times and market,and succeed through improving the quality. 展开更多
关键词 Fritillaria thunbergii miq Current Situation Yinzhou District PRODUCTION aNaLYSIS
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Comparison between Sulfuric Acid-phenol and Sulfuric Acid-anthrone Methods Used for Determination of Polysaccharides in Shoots of Aralia elata (Miq.) Seem. 被引量:2
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作者 Donghai CHU Zhanbo HUANG Fengwei HE 《Agricultural Biotechnology》 CAS 2018年第3期170-173,共4页
Sulfuric acid-phenol and sulfuric acid-anthrone methods were used to detect polysaccharide content in shoots of Aralia elata( Miq.) Seem.,and the conversion factor to glucose was measured with refined polysaccharide... Sulfuric acid-phenol and sulfuric acid-anthrone methods were used to detect polysaccharide content in shoots of Aralia elata( Miq.) Seem.,and the conversion factor to glucose was measured with refined polysaccharides. Comprehensive evaluation was carried out by linear relationship,precision,reproducibility,stability and recovery rate. The results showed that the linear relationship between glucose concentration and absorbance was good when glucose concentration was0-40 μg/ml,and the average recovery rate was equal to or higher than 97. 00% with good reproducibility( RSD 〈 1. 60%,n = 5). It revealed that the two methods were accurate and reliable,and suitable for the determination of polysaccharide content in the shoots of A. elata. Polysaccharide content detected by sulfuric acid-phenol and sulfuric acid-anthrone methods was 19. 31% and 20. 40% respectively. 展开更多
关键词 Shoots of aralia elata miq.) Seem. POLYSaCCHaRIDES Sulfuric acid-phenol method Sulfuric acid-anthrone method
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Study on Endophytic Bacteria Diversity in the Root of Peperomia dindygulensis Miq. by Applying Clone Library Construction Method 被引量:1
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作者 Song JIN Zebin CHEN +3 位作者 Yuchuan LI Tiyuan XIA Zhen REN Lifang YIN 《Medicinal Plant》 2017年第6期13-16,共4页
[Objectives] To study the endophytic bacteria diversity in the root of Peperomia dindygulensis Miq. [Methods] The root of P.dindygulensis Miq. was taken as materials,culture-independent method was applied to build 16 ... [Objectives] To study the endophytic bacteria diversity in the root of Peperomia dindygulensis Miq. [Methods] The root of P.dindygulensis Miq. was taken as materials,culture-independent method was applied to build 16 S rDNA clone library for endophytic bacteria of P. dindygulensis Miq. [Results] In the 16 S rDNA clone library for endophytic bacteria of P. dindygulensis Miq.,95 clones separately belonged to 46 OTUs,Phylogenetic analysis indicated that these cloning sequences separately belonged to phylum Proteobacteria,phylum Bacteroidetes,phylum Actinobacteria,phylum Nitrospirae,and phylum Gemmatimonadetes. In these,phylum Proteobacteria accounted for 70. 53% of total number of clones,so it was the most dominant group in this library. [Conclusions]The endophytic bacteria diversity in the root of P.dindygulensis Miq. is rich and this study is expected to provide scientific basis for protection,development and use of resources of P. dindygulensis Miq. 展开更多
关键词 PEPEROMIa dindygulensis miq ENDOPHYTIC BaCTERIa Culture-independence DIVERSITY
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Effect of extracts from Stachys sieboldii Miq. on cellular reactive oxygen species and glutathione production and genomic DNA oxidation
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作者 JW Lee W Wu SY Lim 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2018年第10期485-489,共5页
Objective: To evaluate the antioxidant activity of extracts and fractions from Stachys sieboldii Miq., and to examine its effect on the cellular reactive oxygen species(ROS) and glutathione(GSH) production and genomic... Objective: To evaluate the antioxidant activity of extracts and fractions from Stachys sieboldii Miq., and to examine its effect on the cellular reactive oxygen species(ROS) and glutathione(GSH) production and genomic DNA oxidation in HT-1080 cells. Methods: The ROS generation induced by H2 O2 was measured by the dichlorofluorescein-diacetate assay. GSH levels were measured using a fluorescent method with mBBr. Genomic DNA oxidative damage was measured with levels of oxidative DNA induced by the reaction of ferritin with H2 O2. Results: The n-hexane, 85% aqueous methanol and n-butanol fractions(0.05 mg/mL concentrations) inhibited H2 O2-induced ROS generation by 63%, 35% and 45%, respectively. GSH levels were significantly increased in both acetone+methylene chloride and methanol extracts(P<0.05). Supplementation of cells with n-hexane significantly increased GSH levels at concentrations of 0.05 mg/mL(P<0.05). Both the acetone+methylene chloride and methanol extracts, as well as all fractions significantly inhibited oxidative DNA damage(P<0.05). Conclusions: These results indicate that cellular oxidation was inhibited by the n-hexane fraction and this fraction may contain valuable active compounds. 展开更多
关键词 STaCHYS sieboldii miq. Reactive oxygen species GLUTaTHIONE DNa OXIDaTION aNTIOXIDaNT
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A New Flavanone from the Bark of Morus macoura Miq.
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作者 Sheng Guo SUN Ruo Yun CHEN De Quan YU 《Chinese Chemical Letters》 SCIE CAS CSCD 2001年第3期231-232,共2页
A new flavanone named macrouroine C (1) was isolated from the bark of Morus macroura Miq.. The structure of 1 was elucidated mainly on the basis of spectroscopic evidence.
关键词 Morus macroura miq. FLaVaNONE macrourone C.
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Effects of Different Harvest Periods on Seed Quality of Alpinia oxyphylla Miq.
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作者 Hanting CHENG Jingkun LIU +2 位作者 Tingliang YAN Qiaoyan ZHANG Jinchuang WANG 《Medicinal Plant》 CAS 2018年第2期52-56,82,共6页
[Objectives] To clarify the appropriate harvest period of Alpinia oxyphylla Miq. seed and improve the quality of seeds. [Methods] In this experiment,the basic parameters and germination characteristics of A. oxyphylla... [Objectives] To clarify the appropriate harvest period of Alpinia oxyphylla Miq. seed and improve the quality of seeds. [Methods] In this experiment,the basic parameters and germination characteristics of A. oxyphylla Miq. seeds during different harvest periods were analyzed and compared. [Results] The results showed that the A. oxyphylla Miq. fruit picking period was closely related to the seed quality,and the seed maturity was highest 100 d after the flowering of A. oxyphylla Miq.,that is,the A. oxyphylla Miq. fruit skin turned from green to yellow( yellow-green),the peel had a small number of brown spots,it was spicy enough,the fruit dry/fresh weight ratio was greater than0. 34,the seed thousand kernel weight reached 11 g or more,and the dried seed moisture content was 13. 01%. Using the TTC method,the seed viability index was determined to be 85%,and in this case,it was the best time of harvesting seed. The water permeability of A. oxyphylla Miq. seed was poor,and the seed imbibition and water absorption time was long,about 72 h. The maximum water absorption rate was about23. 71% at the imbibition and water absorption stage,and it was inferred that the A. oxyphylla Miq. seeds were hard seeds. The germination time of A. oxyphylla Miq. seeds was long,the mature A. oxyphylla Miq. seeds began to germinate on the 12 th d,and the germination rate reached 75. 56% on the 50 th d. [Conclusions] This study determined the optimum harvest period of A. oxyphylla Miq. seeds,clarified the biological characteristics of slow seed water absorption and long germination cycle,and concluded that the seed coat was the main factor leading to slow seed water absorption and long seed germination cycle. 展开更多
关键词 alpinia oxyphylla miq GERMINaTION Harvest period Water absorption rate
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Total Saponins of Aralia elata (Miq.) Seem Leaves (TSAESL) Inhibits Tumor Cell Proliferation and Induces Apoptosis via activating MAPK signaling pathways in MCF-7 human breast cancer cells
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《中国药理学通报》 CAS CSCD 北大核心 2015年第B11期224-225,共2页
Aim Aralia elata (Miq.) Seem is a medicinal plant with a variety of biological activities. Several trier- pene saponins extracted from the leaves of Aralia elata (Miq.) Seem possess potent cytotoxic activity, but ... Aim Aralia elata (Miq.) Seem is a medicinal plant with a variety of biological activities. Several trier- pene saponins extracted from the leaves of Aralia elata (Miq.) Seem possess potent cytotoxic activity, but the mechanisms remain unclear. Methods In the present study, we examined the anti-tumor activity of total saponins of Aralia elata (Miq.) Seem leaves (TSAESL) using both in vitro and in vivo experiments, and investigated the underlying molecular mechanisms. Results Briefly, TSAESL significantly inhibited the proliferation of five human cancer cell lines and induced apoptosis of MCF-7 cells in a concentration-dependent manner. TSAESL also in- creased the expression level of active caspase-3 and cleaved PARP. In addition, TSAESL dramatically activated the phosphorylation of ERK1/2, p38 and JNK in a concentration-dependent manner. Treatment with the ERK1/2 in- hibitor U0126, p38 inhibitor SB203580, or JNK inhibitor SP600125 prior to TSAESL exposure markedly attenuated TSAESL-induced phosphorylation of ERK1/2 and p38. In mice bearing MCF-7 xenografl, TSAESL markedly inhib- ited tumor growth without significantly affecting spleen coefficient and hematological parameters. Conclusion These results strongly suggest that TSAESL has anti-tumor effect via activating the MAPK-mediated signaling path- ways. 展开更多
关键词 aRaLIa elata (miq.) SEEM Human breast cancer aPOPTOSIS MaPK Xenografted NUDE mice
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Temperature Effects on Seed Dormancy Relieving of Fritillaria thunbergii Miq
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作者 高文远 李志亮 肖培根 《Journal of Chinese Pharmaceutical Sciences》 CAS 1997年第3期48-52,共5页
The seed dormancy relieving of Fritillaria thunbergii Miq required 50 days at 8~10℃and 80 days at 3~5℃ In order to study the physiological basis for seed dormancy relieving,peroxidase,acid phosphatase and protei... The seed dormancy relieving of Fritillaria thunbergii Miq required 50 days at 8~10℃and 80 days at 3~5℃ In order to study the physiological basis for seed dormancy relieving,peroxidase,acid phosphatase and proteins were assayed by spectrophotometric analysis and electrophoresis The results showed that peroxidase activity and acid phosphatase activity increased and new bands were detected in protein pattems as the seeds were treated with low temperature,these changes resulted in the seed dormancy relieving 展开更多
关键词 Fritillaria thunbergii miq DORMaNCY PEROXIDaSE acid phosphatase PROTEIN
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Optimization of Extraction Process of Polysaccharides from Actinidia arguta(Sieb.&Zucc.)Planch.ex Miq.
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作者 Xiaohui LI Lihua MA +1 位作者 Lidong YIN Tao LUO 《Medicinal Plant》 CAS 2021年第3期60-61,83,共3页
[Objectives]To optimize the extraction process of polysaccharides from Actinidia arguta(Sieb.&Zucc.)Planch.ex Miq.[Methods]On the basis of single-factor tests,an orthogonal test was conducted with solid/liquid rat... [Objectives]To optimize the extraction process of polysaccharides from Actinidia arguta(Sieb.&Zucc.)Planch.ex Miq.[Methods]On the basis of single-factor tests,an orthogonal test was conducted with solid/liquid ratio,ultrasonic temperature,ultrasonic time and ultrasonic power as influencing factors.[Results]The optimal extraction process was as follows:solid/liquid ratio 1∶15(g/mL),ultrasonic temperature 50℃,ultrasonic time 50 min and ultrasonic power 90 W.Under the optimal extraction conditions,the extraction rate of polysaccharides reached 2.05%.[Conclusions]The method is simple and feasible,and can be used to optimize the extraction process of polysaccharides in A.arguta,in order to provide a theoretical basis for the further development and utilization of related resources. 展开更多
关键词 actinidia arguta(Sieb.&Zucc.)Planch.ex miq. POLYSaCCHaRIDE Extraction process
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山银柴胡质量标准研究
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作者 张旭 张飞 +3 位作者 郭凯华 田志浩 郜志宁 陈随清 《中药材》 CAS 北大核心 2024年第1期117-123,共7页
目的:研究山银柴胡质量评价标准,建立指纹图谱分析方法。方法:参照2020年版中国药典的方法测定山银柴胡水分、总灰分、酸不溶性灰分、醇溶性浸出物含量;采用HPLC法测定山银柴胡中成分含量;建立30批山银柴胡的指纹图谱,结合相似度评价、... 目的:研究山银柴胡质量评价标准,建立指纹图谱分析方法。方法:参照2020年版中国药典的方法测定山银柴胡水分、总灰分、酸不溶性灰分、醇溶性浸出物含量;采用HPLC法测定山银柴胡中成分含量;建立30批山银柴胡的指纹图谱,结合相似度评价、聚类分析、正交偏最小二乘判别分析(OPLS-DA)进行质量评价。结果:30批山银柴胡的水分、总灰分、酸不溶性灰分、醇溶性浸出物、丝石竹皂苷元3-O-β-D-葡萄糖醛酸甲酯、丝石竹皂苷元、皂皮酸含量的平均值分别为8.89%、12.67%、0.79%、34.53%、13.58 mg/g、1.62 mg/g、1.03 mg/g;指纹图谱确定了17个共有峰,样品与对照图谱的相似度为0.916~0.997,聚类分析可将样品分为3类,OPLS-DA分析筛选出7个质量差异性成分。结论:该研究建立的方法可为山银柴胡质量标准的完善提供参考。 展开更多
关键词 山银柴胡 长蕊石头花 细叶石头花 质量标准 指纹图谱
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石榕树中补骨脂素分离鉴定及其抗真菌活性
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作者 邓志勇 黄晞灵 +2 位作者 祝泽宇 黄思雨 邓业成 《山西农业大学学报(自然科学版)》 CAS 北大核心 2024年第5期131-140,共10页
[目的]分离出石榕树中能够有效抑制植物病原真菌的活性单体化合物并探索其抗真菌活性。[方法]采用生物活性追踪和化学分离相结合的方法对石榕树甲醇提取物进行分离,运用质谱、核磁共振氢谱和碳谱并结合相关文献数据鉴定活性单体化合物... [目的]分离出石榕树中能够有效抑制植物病原真菌的活性单体化合物并探索其抗真菌活性。[方法]采用生物活性追踪和化学分离相结合的方法对石榕树甲醇提取物进行分离,运用质谱、核磁共振氢谱和碳谱并结合相关文献数据鉴定活性单体化合物。采用菌丝生长速率法测定活性单体化合物对7种植物病原真菌的抑菌活性。[结果]石榕树(叶)甲醇提取物对甘蔗凤梨病菌具有良好抑菌活性,在10 mg·mL^(-1)浓度下,抑菌率为100%。在浓度为1.5 mg·mL^(-1)时,石榕树(叶)石油醚、乙酸乙酯、正丁醇萃取物和水层萃余物对甘蔗凤梨病菌的抑菌率分别为58.73%、100.00%、39.49%和6.63%。表明石榕树(叶)乙酸乙酯层萃取物对甘蔗凤梨病菌的抑制作用最高,其EC_(50)值为0.430 7 mg·mL^(-1)。石榕树(叶)乙酸乙酯层萃取物进行柱色谱分离后得到对甘蔗凤梨病菌有较高抑菌活性的是LMNO1bc1a活性组分,0.1 mg·mL^(-1)的LMNO1bc1a活性组分对甘蔗凤梨病菌的抑菌率为100.00%。LMNO1bc1a组分经结晶、重结晶纯化后命名为SRS-1,SRS-1经NMR和MS技术鉴定为补骨脂素(psoralen)。SRS-1对甘蔗凤梨病菌、甘蓝黑斑病菌、玉米大斑病菌、罗汉果白绢病菌、柑橘砂皮病菌、茶轮斑病菌、烟草黑胫病菌这7种植物病原真菌都有一定的抑菌活性,其EC_(50)值分别为0.018 2、0.038 8、0.040 9、0.051 2、0.080 7、0.092 7、0.115 4 mg·mL^(-1)。[结论]从石榕树中分离出抑菌活性单体化合物SRS-1,鉴定为补骨脂素,其对甘蔗凤梨病菌、甘蓝黑斑病菌、玉米大斑病菌、罗汉果白绢病菌、柑橘砂皮病菌、茶轮斑病菌、烟草黑胫病菌这7种植物病原真菌都有一定的抑菌活性。 展开更多
关键词 石榕树 补骨脂素 分离 鉴定 植物病原真菌 抑菌活性
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黑龙江省延寿县驯化软枣猕猴桃品种果实品质比较分析 被引量:2
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作者 孔德馨 姜雅轩 +3 位作者 张佩远 雷培 张世凯 孟凡娟 《北方园艺》 CAS 北大核心 2024年第5期9-16,共8页
以2022年10月1日于黑龙江省延寿县长寿山庄采摘4种野生驯化的软枣猕猴桃品种(“果佬一号”“果佬二号”“东林一号”和“东林二号”)的果实和叶片为试材,采用多种生理指标测定方法,研究了果实品质特征,叶片、果实及植株的形态特征,以期... 以2022年10月1日于黑龙江省延寿县长寿山庄采摘4种野生驯化的软枣猕猴桃品种(“果佬一号”“果佬二号”“东林一号”和“东林二号”)的果实和叶片为试材,采用多种生理指标测定方法,研究了果实品质特征,叶片、果实及植株的形态特征,以期为今后的广泛推广种植提供参考依据。结果表明:“果佬一号”和“果佬二号”果实较大,其果实平均鲜质量较高,分别为41.65 g和42.55 g。“果佬一号”和“东林二号”具有较高的可溶性糖含量,分别为6.19 mg·g^(-1)FW和6.21 mg·g^(-1)FW;“果佬二号”的维生素C含量最高为48.07μmol·g^(-1)FW;“果佬二号”和“东林二号”的可溶性固形物含量约比其他2个品种高10%;“果佬一号”和“东林一号”的可滴定酸含量约比其他2个品种高46%;“果佬二号”的固酸比最高为30.75。综上所述,4种驯化的软枣猕猴桃中,“果佬一号”产量较高且品质也较好,是综合性状均表现优良的品种;而“果佬二号”的果实红芯、适口性较好,同时也具有多个综合的优良品质,适宜推广;此外“东林一号”果实耐储运、红芯、植株颜色多变,既可用于酿酒或制作果醋,也可以用于观赏;“东林二号”果实红芯、品质好、产量适中,也较适合推广。 展开更多
关键词 软枣猕猴桃 果实 品质
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基于AFLP标记技术的三翅槭与三角枫亲缘关系分析 被引量:8
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作者 程小毛 岳琳 黄晓霞 《中国农学通报》 CSCD 北大核心 2011年第16期79-83,共5页
为了从分子水平上揭示三翅槭与三角枫的亲缘关系,明确三翅槭在槭属中的分类地位,利用AFLP分子标记技术对三翅槭1份材料和三角枫4份材料进行亲缘关系检测,并采用UPGMA法进行聚类分析。结果表明:8对引物组合共获得202条谱带,其中99条带具... 为了从分子水平上揭示三翅槭与三角枫的亲缘关系,明确三翅槭在槭属中的分类地位,利用AFLP分子标记技术对三翅槭1份材料和三角枫4份材料进行亲缘关系检测,并采用UPGMA法进行聚类分析。结果表明:8对引物组合共获得202条谱带,其中99条带具有多态性,多态性比率为49.01%,说明AFLP标记技术适用于三翅槭和三角枫亲缘关系研究。聚类分析表明,在遗传相似系数为0.71时,可将三翅槭和三角枫划分成2个相互独立的类群,说明三翅槭与三角枫在分子水平上的分类与形态学上的分类一致,此外三翅槭与三角枫之间的遗传相似系数为0.71也表明它们之间的亲缘关系较近。通过AFLP分子标记,成功分析了三翅槭与三角枫亲缘关系,为证明三翅槭是槭属新种提供了分子证据。 展开更多
关键词 三翅槭 三角枫 aFLP 亲缘关系
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