Objective:To investigate the hepatoprotective activity of methanolic leaf extract of Cyathea gigantea(C.gigantea)against paracetamol induced liver damage in rats.Methods:The hepatoprotective activity for plant extract...Objective:To investigate the hepatoprotective activity of methanolic leaf extract of Cyathea gigantea(C.gigantea)against paracetamol induced liver damage in rats.Methods:The hepatoprotective activity for plant extract was investigated for paracetamol induced hepatoxicity in rats.Wislar albino rats of either sex were divided into five groups of 6 animals each and are given orally the following treatment for seven days.The normal control group was given 1%Na.CMC 1mL/kg bw,p.o.Paracetamol at dose of 1g/kg bw,p.o.was given as toxic dose for inducing hepatoloxicity.Silymarin(50mg/kg.p.o.) was given as reference standard.Two doses of C. gigantea extract i.e.,100 mg/kg.p.o.and 200 mg/kg,p.o.were tested for hepatoprotective activity. The treatment was given for seven days and after 24 h of last treatment blood was collected from retro-orbital plexus and analysed for various serum parameters like serum glutamic-oxaloacetic transaminase(SGOT),serum glutamic pyruvic transaminase(SGPT),alkaline phosphatase(ALP),total bilirubin(TB)and total protein(TP)in different groups.Results:The paracetamol intoxication lead to histological and biochemical deteriorations.The treatment with methanolic leaf extract of C.gigantea reduced the elevated levels of SCOT,SGPT,ALP,TB and also reversed the hepatic damage towards normal which further supports the hepatoprotective activity of leaf extract of C.gigantea.Conclusions:The methanolic extract of leaves of C.gigantea at doses of 100 mg/kg bw and 200 mg/kg bw have significant effect on liver of paracetamol induced hepatotoxicity model in rats.展开更多
Objective:To investigate experimentally the possible antitumor effect of methanol extract(ME) of Calotropis gigantea L.(C.gigantean) root bark and its petroleum ether(PEF) and chloroform(CF) soluble fractions against ...Objective:To investigate experimentally the possible antitumor effect of methanol extract(ME) of Calotropis gigantea L.(C.gigantean) root bark and its petroleum ether(PEF) and chloroform(CF) soluble fractions against Ehrlich ascites carcinoma(EAC) in Swiss albino mice.Methods:The effects of ME(10 and 20 mg/kg),PEF(40 and 80 mg/kg) and CF(20 and 40 mg/kg) on the growth of EAC and life span of EAC bearing mice were studied.Hematological profile and biochemical parameters(SAI.P,SGPT and SCOT) were also estimated.Results:Results of in vivo study showed a significant decrease in viable tumor cell count and a significant increase of life span in the ME and CF treated group compared to untreated one.The life span of ME and CF treated animals was significandy(P【0.05) increased by 43.90%(20 mg ME/kg) and 57.07%(40 mg CF/kg).ME and CF brought back the hematological parameter more or less normal level.ME and CF also restored the altered levels of serum alkaline phosphatase(SALP) and serum glutamate oxaloacetate transaminase(SGOT).Conclusions:Methanol extract(ME) of C.gigantea root bark and its chloroform soluble fraction(CF) possesses significant antitumor activity.展开更多
Temperature was one of the key factors for the living environment of aquatic organisms, especially for shellfish. The effects of temperature was studied on the survival rate and the median lethal time （LT50） of the ...Temperature was one of the key factors for the living environment of aquatic organisms, especially for shellfish. The effects of temperature was studied on the survival rate and the median lethal time （LT50） of the rock scallop Crassadoma gigantea, and the effects of temperature stress on the antioxidant enzymes of rock scallop Crassadoma gigantea were studied in order to explore the effects of temperature on keeping-alive without water and antioxidant system of the rock scallop Crassadoma gigantea. The rock scallop Crassadoma gigantea （shell length （39.7± 5） mm） at different temperatures （5℃ and 15℃）, humidity （moisture treatment, not moisturizing treatment） and oxygen （aerobic treatment, not aerobic treatment） in laboratory was studied by the survival analysis and the analysis of factors affecting the effects of the median lethal time （LTso） in order to investigate the effects of temperature on the survival rate and the median lethal time of the rock scallop Crassadoma gigantea. Results showed that low temperature, moisture and aerobic treatment can significantly improve the survival rate and the median lethal time of the rock scallop Crassadoma gigantea （P〈0.05）. The effect of temperature on the survival rate and the median lethal time of the rock scallop Crassadoma gigantea was most significant, followed by the effect of oxygen, and that of humidity was the most weakest. Interaction between temperature and humidity, temperature and oxygen significant interaction（P〉0.05）, and interaction between humidity and oxygen interaction was not significant （P〈0.05） in the interaction of three factors. It provided an important basis for the determination of the rock scallop Crassadoma gigantea in the seedling transportation time. In the article, the rock scallop Crassadoma gigantea larvae antioxidant enzymes activity was detected, including SOD activity,CAT activity ,T-A℃ activity （superoxide dismutase activity, catalase activity, total antioxidant capacity） changes through raising the temperature from the low temperature （5℃, 10℃, 15℃, 20℃, 25℃）, to explore the change of the antioxidant enzymes activity under the influence of temperature on the rock scallop Crassadoma gigantea larvae. The SODactivity was significantly lower than that of the other groups （P〈0.05） at 5℃, and the CAT activity had no significant difference between groups （p=0.05）, and the T - AOC level was the lowest significantly below 15℃, 25℃ （P〈0.05）.The SOD and CAT activity had no significant difference in the same experimental time （P〉0.05）, and the T - AOC activity temperature was significantly higher than the rest of the group （P=〈0.05） when temperature at 10℃ and 20℃. The SOD activity had no significant difference （P〉0.05）between groups at 25℃, and the CAT activity was significantly higher than 5℃ 10℃, 15℃ group （P〈0.05）. The T-AOC activity was only lower than 15℃ group （P〉0.05）. It indicated that the high temperature had a clear inducing effect on the CAT activity in the rock scallop Crassadoma gigantea larvae, and had a significant inhibitory effect on the T-AOC activity. The low temperature had a significant inhibitory effect on the activity of T-AOC in the tissue fluid of the scallop. The above results showed that the high and low temperature had a significant effect on the antioxidant enzymes activity of the rock scallop Crassadoma gigantea larvae.展开更多
Based on artificial forest of Calotropis gigantea (L.) Dryand. area of 20.1 hm2, planted in the base of seed management station in Yuanjiang County, Yunnan Province, according to the average standard wooden method, th...Based on artificial forest of Calotropis gigantea (L.) Dryand. area of 20.1 hm2, planted in the base of seed management station in Yuanjiang County, Yunnan Province, according to the average standard wooden method, the stand biomass was calculated in this paper. Based on the survey data of representative sample trees, the single-tree biomass model was constructed.展开更多
Objective:To characterize the antifungal activity of methanolic leaf extract of Calotropis gigantea alone or in combination with amphotericin B against invasive pulmonary aspergillosis in mice.Methods:GC/MS was used f...Objective:To characterize the antifungal activity of methanolic leaf extract of Calotropis gigantea alone or in combination with amphotericin B against invasive pulmonary aspergillosis in mice.Methods:GC/MS was used for analysis of active constituents of Calotropis gigantea extract.Spore germination assay and broth micro-dilution method were used to determine antifungal potential of Calotropis gigantea/amphotericin B against Aspergillus fumigatus.Neutropenic mice were randomly assigned into 5 groups:group 1 was neutropenic(control);group 2 was infected with Aspergillus fumigatus;group 3 was infected with Aspergillus fumigatus,and treated with Calotropis gigantea extract;group 4 was infected with Aspergillus fumigatus and treated with amphotericin B;group 5 was infected with Aspergillus fumigatus and treated with both Calotropis gigantea extract and amphotericin B.Fresh lung tissues were histopathologically examined.Fungal burden and gliotoxin concentration were evaluated in lung tissues.Catalase,superoxide dismutase,and malondialdehyde content were determined in lung tissues.Myeloperoxidase,tumor necrosis factor-alpha,interleukin-1,and interleukin-17 were also estimated by the sandwich enzyme-linked immuno-sorbent assay.Results:Calotropis gigantea/amphotericin B had a minimum inhibitory concentration and minimum fungicidal concentration of 80 and 160μg/mL,respectively,for Aspergillus fumigatus.Additionally,Calotropis gigantea/amphotericin B significantly reduced lung fungal burden by 72.95%and inhibited production of gliotoxin in lung tissues from 6320 to 1350μg/g lung.Calotropis gigantea/amphotericin B reduced the oxidative stress of the lung via elevating the activity of antioxidant enzymes and decreasing the levels of lipid peroxidation.Myeloperoxidase activity and the production of pro-inflammatory cytokines were also significantly reduced.Scanning electron microscopy revealed deteriorations in the hyphae ultrastructure in Calotropis gigantea/amphotericin B treated Aspergillus fumigatus and leak of cellular components after damage of the cell wall.In vivo study revealed the suppression of lung tissue damage in mice of invasive pulmonary aspergillosis,which was improved with Calotropis gigantea/amphotericin B compared to the control group.Conclusions:Calotropis gigantea/amphotericin B is a promising treatment to reduce lung fungal burden and to improve the drugs’therapeutic effect against invasive pulmonary aspergillosis.展开更多
In order to study the effect of temperature on the growth of juvenile rock scallop(Crassadoma gigantea),learn more about its growth with temperature changes under the conditions of indoor circulating water temperature...In order to study the effect of temperature on the growth of juvenile rock scallop(Crassadoma gigantea),learn more about its growth with temperature changes under the conditions of indoor circulating water temperature-controlled culture.Four temperature gradients of10℃,12.5℃,15℃and17.5℃were set,observed the growth of juvenile rock scallop at different test temperatures during two month.The results show:With the increase of test temperature,the growth rate first increased and then decreased.One-way analysis of variance showed that temperature had a significant effect on the growth rate(P<0.05),the optimum temperature was15℃,under this condition,the shell height average daily growth was significantly higher than the other three groups,the average daily growth of shell length and wet weight had no significant difference with17.5℃experimental group but significantly higher than the previous two groups.Within the experimental range,the change of temperature had no significant effect on the survival rate of juvenile rock scallop(P>0.05).展开更多
[Objectives] To effectively develop Calotropis gigantea resources and find the biological active constituents with medicinal value.[Methods]The liposoluble constituents from C. gigantea roots were studied by GC-MS for...[Objectives] To effectively develop Calotropis gigantea resources and find the biological active constituents with medicinal value.[Methods]The liposoluble constituents from C. gigantea roots were studied by GC-MS for the first time. [Results] 62 constituents were separated and identified from C. gigantea roots,amounting to 60. 43% of the total constituents,α-amyrin( 4. 18%),ethyl palmitate( 3. 49%),1,7-dimethyl-naphthalene( 3. 06%) were the main liposoluble constituents. [Conclusions] Cardiac glycoside was mainly found in methanol extracts and not detected in liposoluble constituents by extraction of petroleum ether. However,the present study found another component of low toxicity—naphthalene and its derivatives.展开更多
[Objectives] To study the liposoluble constituents and their antimicrobial activity from the flowers of Calotropis gigantea.[Methods]The liposoluble constituents from the flowers of Calotropis gigantea were analyzed b...[Objectives] To study the liposoluble constituents and their antimicrobial activity from the flowers of Calotropis gigantea.[Methods]The liposoluble constituents from the flowers of Calotropis gigantea were analyzed by gas chromatography-mass spectrometry(GCMS) method and their relative content was determined by area normalization method; and their antimicrobial activity against 2 pathogens was tested by agar disc diffusion method. [Results]31 compounds were separated and identified,amounting to 85. 14% of the total constituents,27 compounds were reported for the first time in the study of the liposoluble constituents from Calotropis gigantea. The results indicated that the liposoluble constituents from the flowers of Calotropis gigantea exhibited weak antimicrobial activity against Staphylococcus aureus and Canidia albicans. [Conclusions] The research results provided scientific foundation for exploitation and utilization of Calotropis gigantea.展开更多
Phlebiopsis gigantea strains registered in the European Union as biocontrol agents against root rot in forests （four from Great Britain and two from Finland） were compared using Ward＇s method with reference to： （...Phlebiopsis gigantea strains registered in the European Union as biocontrol agents against root rot in forests （four from Great Britain and two from Finland） were compared using Ward＇s method with reference to： （l） similarity of DNA-random amplified microsatellite （RAMS） ladders, （2） cellulase and peroxidase production and （3） acceleration of dry mass wood loss in Norway spruce The activity of the enzymes was tested in the initial phase of wood decay （30 d after inoculation） and indicated as the most active isolates： VFI0 and FCl5 for cellulase and FC16 and VFI0 for peroxidase production. The assessment of loss of wood six months after inoculation indicated isolate FC 15 as the most active. P. gigantea isolates similar in terms of enzyme activity indicated different patterns ofDNA microsatellite loci. At the same time, DNA-RAMS revealed similarities in isolates with different abilities to produce enzymes. However, some similarities and differences between isolates according to wood decay were found. No plain relationships between molecular characteristics and enzyme activity of the strains tested were observed. The results differentiated activity of tested isolates and suggested benefits of selecting P. gigantea strains for commercial use basing mainly on the assessment of wood loss activity.展开更多
Objective: Rosa odorata var. gigantea is a popular medicinal plant. Some studies have demonstrated that ethanolic extract of the fruits of R. odorata var. gigantea(FOE) has gastroprotective properties. The aim of this...Objective: Rosa odorata var. gigantea is a popular medicinal plant. Some studies have demonstrated that ethanolic extract of the fruits of R. odorata var. gigantea(FOE) has gastroprotective properties. The aim of this study was to investigate the gastroprotective activity of FOE on water immersion restrained stress(WIRS)-induced gastric mucosal injury in a rat model and elucidate the possible molecular mechanisms involved.Methods: A rat stress ulcer model was established in this study using WIRS. After rats were treated with FOE orally for 7 d, the effect of FOE treatment was analyzed by hematoxylin and eosin(H&E) staining, and the changes of inflammatory factors, oxidative stress factors, and gastric-specific regulatory factors and pepsin in the blood and gastric tissues of rats were examined by ELISA assay. Molecular mechanism of FOE was investigated by immunohistochemical assay and Western blot.Results: Compared with the WIRS group, FOE could diminish both the macroscopic and microscopic pathological morphology of gastric mucosa. FOE significantly preserved the antioxidants glutathione peroxidase(GSH-PX), superoxide dismutase(SOD) and catalase(CAT) contents;anti-inflammatory cytokines interleukin-10(IL-10) and prostaglandin E2(PGE2) levels as well as regulatory factors tumor necrosis factor-a(TGF-a) and somatostatin(SS) contents, while decreasing malondialdehyde(MDA), nitric oxide synthase(i NOS), tumor necrosis factor(TNF-a), interleukin-1β(IL-1β), interleukin-6(IL-6), gastrin(GAS)and endothelin(ET) levels. Moreover, FOE distinctly upregulated the expression of Nrf2, HO-1, Bcl2 and proliferating cell nuclear antigen(PCNA). In addition, FOE activated the expression of p-EGFR and downregulated the expression of NF-ΚB, Bax, Cleaved-caspase-3, Cyto-C and Cleaved-PARP1, thus promoting gastric mucosal cell survival.Conclusion: The current work demonstrated that FOE exerted a gastroprotective activity against gastric mucosal injury induced by WIRS. The underlying mechanism might be associated with the improvement of anti-inflammatory, anti-oxidation and anti-apoptosis systems.展开更多
基金Supported by University Grants Commission.(No.Fdb/Pharmacy/C6102/2008-2010/5162)
文摘Objective:To investigate the hepatoprotective activity of methanolic leaf extract of Cyathea gigantea(C.gigantea)against paracetamol induced liver damage in rats.Methods:The hepatoprotective activity for plant extract was investigated for paracetamol induced hepatoxicity in rats.Wislar albino rats of either sex were divided into five groups of 6 animals each and are given orally the following treatment for seven days.The normal control group was given 1%Na.CMC 1mL/kg bw,p.o.Paracetamol at dose of 1g/kg bw,p.o.was given as toxic dose for inducing hepatoloxicity.Silymarin(50mg/kg.p.o.) was given as reference standard.Two doses of C. gigantea extract i.e.,100 mg/kg.p.o.and 200 mg/kg,p.o.were tested for hepatoprotective activity. The treatment was given for seven days and after 24 h of last treatment blood was collected from retro-orbital plexus and analysed for various serum parameters like serum glutamic-oxaloacetic transaminase(SGOT),serum glutamic pyruvic transaminase(SGPT),alkaline phosphatase(ALP),total bilirubin(TB)and total protein(TP)in different groups.Results:The paracetamol intoxication lead to histological and biochemical deteriorations.The treatment with methanolic leaf extract of C.gigantea reduced the elevated levels of SCOT,SGPT,ALP,TB and also reversed the hepatic damage towards normal which further supports the hepatoprotective activity of leaf extract of C.gigantea.Conclusions:The methanolic extract of leaves of C.gigantea at doses of 100 mg/kg bw and 200 mg/kg bw have significant effect on liver of paracetamol induced hepatotoxicity model in rats.
文摘Objective:To investigate experimentally the possible antitumor effect of methanol extract(ME) of Calotropis gigantea L.(C.gigantean) root bark and its petroleum ether(PEF) and chloroform(CF) soluble fractions against Ehrlich ascites carcinoma(EAC) in Swiss albino mice.Methods:The effects of ME(10 and 20 mg/kg),PEF(40 and 80 mg/kg) and CF(20 and 40 mg/kg) on the growth of EAC and life span of EAC bearing mice were studied.Hematological profile and biochemical parameters(SAI.P,SGPT and SCOT) were also estimated.Results:Results of in vivo study showed a significant decrease in viable tumor cell count and a significant increase of life span in the ME and CF treated group compared to untreated one.The life span of ME and CF treated animals was significandy(P【0.05) increased by 43.90%(20 mg ME/kg) and 57.07%(40 mg CF/kg).ME and CF brought back the hematological parameter more or less normal level.ME and CF also restored the altered levels of serum alkaline phosphatase(SALP) and serum glutamate oxaloacetate transaminase(SGOT).Conclusions:Methanol extract(ME) of C.gigantea root bark and its chloroform soluble fraction(CF) possesses significant antitumor activity.
文摘Temperature was one of the key factors for the living environment of aquatic organisms, especially for shellfish. The effects of temperature was studied on the survival rate and the median lethal time （LT50） of the rock scallop Crassadoma gigantea, and the effects of temperature stress on the antioxidant enzymes of rock scallop Crassadoma gigantea were studied in order to explore the effects of temperature on keeping-alive without water and antioxidant system of the rock scallop Crassadoma gigantea. The rock scallop Crassadoma gigantea （shell length （39.7± 5） mm） at different temperatures （5℃ and 15℃）, humidity （moisture treatment, not moisturizing treatment） and oxygen （aerobic treatment, not aerobic treatment） in laboratory was studied by the survival analysis and the analysis of factors affecting the effects of the median lethal time （LTso） in order to investigate the effects of temperature on the survival rate and the median lethal time of the rock scallop Crassadoma gigantea. Results showed that low temperature, moisture and aerobic treatment can significantly improve the survival rate and the median lethal time of the rock scallop Crassadoma gigantea （P〈0.05）. The effect of temperature on the survival rate and the median lethal time of the rock scallop Crassadoma gigantea was most significant, followed by the effect of oxygen, and that of humidity was the most weakest. Interaction between temperature and humidity, temperature and oxygen significant interaction（P〉0.05）, and interaction between humidity and oxygen interaction was not significant （P〈0.05） in the interaction of three factors. It provided an important basis for the determination of the rock scallop Crassadoma gigantea in the seedling transportation time. In the article, the rock scallop Crassadoma gigantea larvae antioxidant enzymes activity was detected, including SOD activity,CAT activity ,T-A℃ activity （superoxide dismutase activity, catalase activity, total antioxidant capacity） changes through raising the temperature from the low temperature （5℃, 10℃, 15℃, 20℃, 25℃）, to explore the change of the antioxidant enzymes activity under the influence of temperature on the rock scallop Crassadoma gigantea larvae. The SODactivity was significantly lower than that of the other groups （P〈0.05） at 5℃, and the CAT activity had no significant difference between groups （p=0.05）, and the T - AOC level was the lowest significantly below 15℃, 25℃ （P〈0.05）.The SOD and CAT activity had no significant difference in the same experimental time （P〉0.05）, and the T - AOC activity temperature was significantly higher than the rest of the group （P=〈0.05） when temperature at 10℃ and 20℃. The SOD activity had no significant difference （P〉0.05）between groups at 25℃, and the CAT activity was significantly higher than 5℃ 10℃, 15℃ group （P〈0.05）. The T-AOC activity was only lower than 15℃ group （P〉0.05）. It indicated that the high temperature had a clear inducing effect on the CAT activity in the rock scallop Crassadoma gigantea larvae, and had a significant inhibitory effect on the T-AOC activity. The low temperature had a significant inhibitory effect on the activity of T-AOC in the tissue fluid of the scallop. The above results showed that the high and low temperature had a significant effect on the antioxidant enzymes activity of the rock scallop Crassadoma gigantea larvae.
文摘Based on artificial forest of Calotropis gigantea (L.) Dryand. area of 20.1 hm2, planted in the base of seed management station in Yuanjiang County, Yunnan Province, according to the average standard wooden method, the stand biomass was calculated in this paper. Based on the survey data of representative sample trees, the single-tree biomass model was constructed.
基金the Deanship of Scientific ResearchVice Presidency for Graduate Studies and Scientific Research+1 种基金King Faisal UniversitySaudi Arabia [Project No. GRANT93 (170061)]
文摘Objective:To characterize the antifungal activity of methanolic leaf extract of Calotropis gigantea alone or in combination with amphotericin B against invasive pulmonary aspergillosis in mice.Methods:GC/MS was used for analysis of active constituents of Calotropis gigantea extract.Spore germination assay and broth micro-dilution method were used to determine antifungal potential of Calotropis gigantea/amphotericin B against Aspergillus fumigatus.Neutropenic mice were randomly assigned into 5 groups:group 1 was neutropenic(control);group 2 was infected with Aspergillus fumigatus;group 3 was infected with Aspergillus fumigatus,and treated with Calotropis gigantea extract;group 4 was infected with Aspergillus fumigatus and treated with amphotericin B;group 5 was infected with Aspergillus fumigatus and treated with both Calotropis gigantea extract and amphotericin B.Fresh lung tissues were histopathologically examined.Fungal burden and gliotoxin concentration were evaluated in lung tissues.Catalase,superoxide dismutase,and malondialdehyde content were determined in lung tissues.Myeloperoxidase,tumor necrosis factor-alpha,interleukin-1,and interleukin-17 were also estimated by the sandwich enzyme-linked immuno-sorbent assay.Results:Calotropis gigantea/amphotericin B had a minimum inhibitory concentration and minimum fungicidal concentration of 80 and 160μg/mL,respectively,for Aspergillus fumigatus.Additionally,Calotropis gigantea/amphotericin B significantly reduced lung fungal burden by 72.95%and inhibited production of gliotoxin in lung tissues from 6320 to 1350μg/g lung.Calotropis gigantea/amphotericin B reduced the oxidative stress of the lung via elevating the activity of antioxidant enzymes and decreasing the levels of lipid peroxidation.Myeloperoxidase activity and the production of pro-inflammatory cytokines were also significantly reduced.Scanning electron microscopy revealed deteriorations in the hyphae ultrastructure in Calotropis gigantea/amphotericin B treated Aspergillus fumigatus and leak of cellular components after damage of the cell wall.In vivo study revealed the suppression of lung tissue damage in mice of invasive pulmonary aspergillosis,which was improved with Calotropis gigantea/amphotericin B compared to the control group.Conclusions:Calotropis gigantea/amphotericin B is a promising treatment to reduce lung fungal burden and to improve the drugs’therapeutic effect against invasive pulmonary aspergillosis.
基金supported by Liaoning Provincial Education Department Project(NO.L201624)
文摘In order to study the effect of temperature on the growth of juvenile rock scallop(Crassadoma gigantea),learn more about its growth with temperature changes under the conditions of indoor circulating water temperature-controlled culture.Four temperature gradients of10℃,12.5℃,15℃and17.5℃were set,observed the growth of juvenile rock scallop at different test temperatures during two month.The results show:With the increase of test temperature,the growth rate first increased and then decreased.One-way analysis of variance showed that temperature had a significant effect on the growth rate(P<0.05),the optimum temperature was15℃,under this condition,the shell height average daily growth was significantly higher than the other three groups,the average daily growth of shell length and wet weight had no significant difference with17.5℃experimental group but significantly higher than the previous two groups.Within the experimental range,the change of temperature had no significant effect on the survival rate of juvenile rock scallop(P>0.05).
基金Supported by National Natural Science Foundation of Hainan China(20168368)Species Resources(Tropical Crops)Conservation Project of the Ministry of Agriculture(151721301354052012)
文摘[Objectives] To effectively develop Calotropis gigantea resources and find the biological active constituents with medicinal value.[Methods]The liposoluble constituents from C. gigantea roots were studied by GC-MS for the first time. [Results] 62 constituents were separated and identified from C. gigantea roots,amounting to 60. 43% of the total constituents,α-amyrin( 4. 18%),ethyl palmitate( 3. 49%),1,7-dimethyl-naphthalene( 3. 06%) were the main liposoluble constituents. [Conclusions] Cardiac glycoside was mainly found in methanol extracts and not detected in liposoluble constituents by extraction of petroleum ether. However,the present study found another component of low toxicity—naphthalene and its derivatives.
基金Supported by National Natural Science Foundation of Hainan China(20168368)Agro-scientific Research in the Public Interest(201303117)
文摘[Objectives] To study the liposoluble constituents and their antimicrobial activity from the flowers of Calotropis gigantea.[Methods]The liposoluble constituents from the flowers of Calotropis gigantea were analyzed by gas chromatography-mass spectrometry(GCMS) method and their relative content was determined by area normalization method; and their antimicrobial activity against 2 pathogens was tested by agar disc diffusion method. [Results]31 compounds were separated and identified,amounting to 85. 14% of the total constituents,27 compounds were reported for the first time in the study of the liposoluble constituents from Calotropis gigantea. The results indicated that the liposoluble constituents from the flowers of Calotropis gigantea exhibited weak antimicrobial activity against Staphylococcus aureus and Canidia albicans. [Conclusions] The research results provided scientific foundation for exploitation and utilization of Calotropis gigantea.
文摘Phlebiopsis gigantea strains registered in the European Union as biocontrol agents against root rot in forests （four from Great Britain and two from Finland） were compared using Ward＇s method with reference to： （l） similarity of DNA-random amplified microsatellite （RAMS） ladders, （2） cellulase and peroxidase production and （3） acceleration of dry mass wood loss in Norway spruce The activity of the enzymes was tested in the initial phase of wood decay （30 d after inoculation） and indicated as the most active isolates： VFI0 and FCl5 for cellulase and FC16 and VFI0 for peroxidase production. The assessment of loss of wood six months after inoculation indicated isolate FC 15 as the most active. P. gigantea isolates similar in terms of enzyme activity indicated different patterns ofDNA microsatellite loci. At the same time, DNA-RAMS revealed similarities in isolates with different abilities to produce enzymes. However, some similarities and differences between isolates according to wood decay were found. No plain relationships between molecular characteristics and enzyme activity of the strains tested were observed. The results differentiated activity of tested isolates and suggested benefits of selecting P. gigantea strains for commercial use basing mainly on the assessment of wood loss activity.
基金supported by the National Natural Science Foundation of China(No.81673693)the National Key R&D Program of China(No.2017YFD0201402)for financial support.
文摘Objective: Rosa odorata var. gigantea is a popular medicinal plant. Some studies have demonstrated that ethanolic extract of the fruits of R. odorata var. gigantea(FOE) has gastroprotective properties. The aim of this study was to investigate the gastroprotective activity of FOE on water immersion restrained stress(WIRS)-induced gastric mucosal injury in a rat model and elucidate the possible molecular mechanisms involved.Methods: A rat stress ulcer model was established in this study using WIRS. After rats were treated with FOE orally for 7 d, the effect of FOE treatment was analyzed by hematoxylin and eosin(H&E) staining, and the changes of inflammatory factors, oxidative stress factors, and gastric-specific regulatory factors and pepsin in the blood and gastric tissues of rats were examined by ELISA assay. Molecular mechanism of FOE was investigated by immunohistochemical assay and Western blot.Results: Compared with the WIRS group, FOE could diminish both the macroscopic and microscopic pathological morphology of gastric mucosa. FOE significantly preserved the antioxidants glutathione peroxidase(GSH-PX), superoxide dismutase(SOD) and catalase(CAT) contents;anti-inflammatory cytokines interleukin-10(IL-10) and prostaglandin E2(PGE2) levels as well as regulatory factors tumor necrosis factor-a(TGF-a) and somatostatin(SS) contents, while decreasing malondialdehyde(MDA), nitric oxide synthase(i NOS), tumor necrosis factor(TNF-a), interleukin-1β(IL-1β), interleukin-6(IL-6), gastrin(GAS)and endothelin(ET) levels. Moreover, FOE distinctly upregulated the expression of Nrf2, HO-1, Bcl2 and proliferating cell nuclear antigen(PCNA). In addition, FOE activated the expression of p-EGFR and downregulated the expression of NF-ΚB, Bax, Cleaved-caspase-3, Cyto-C and Cleaved-PARP1, thus promoting gastric mucosal cell survival.Conclusion: The current work demonstrated that FOE exerted a gastroprotective activity against gastric mucosal injury induced by WIRS. The underlying mechanism might be associated with the improvement of anti-inflammatory, anti-oxidation and anti-apoptosis systems.
