To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax graeilistylus W. W. Smith （AGS）, the crystal structure was measured with a Bruker APEX-Ⅱ area-...To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax graeilistylus W. W. Smith （AGS）, the crystal structure was measured with a Bruker APEX-Ⅱ area-detector diffractometer instrument and the technology of extracting in combination hydrolysis in situ （ECHS） was compared with these of traditional methods. The crystal belongs to the monoclinic system, space group P2b with unit cell parameters： a=（8.3652±0.0006） nm, b=（24.721±0.002） nm, and c=（14.5587±0.0011） nm, α=90°, β=97.850 （4） °, γ=90 °, V=2982.51 nm3, Dc= 1.179 mg/m3, and the molecular number （Z） of elementary structures was 2. The comparisons show that the extraction rate of acankoreanogenin with ECHS methods is much higher than that of traditional methods. Then, central composite design-response surface methodology （CCD-RSM） was adopted for optimizing the extraction rate of ECHS methods. The optimized values of extraction parameters are as follows： for the for extraction process of acid hydrolysis are that extraction time 110.8 min, solvent-herb ratio 11.5 and acid content 5.25%; the best extraction process of basic hydrolysis are that extract time 120 min, solvent-herb ratio 8.7 and the alkali content 8.79%. Finally, the extracts were purified with decolorizing carbon after alkali solution and acid-isolation and purity of acankoreanogenin was 98.7%.展开更多
A new triterpenoid, 11a.O.trans-p-coumaroyltaraxerol （1）, along with 11 known triterpenoids, taraxerone （2）, taraxerol （3）, 2α,3β,23,24-tetrahydroxyolean-12-en-28-oic acid （4）, oleanolic acid （5）, β-amyr...A new triterpenoid, 11a.O.trans-p-coumaroyltaraxerol （1）, along with 11 known triterpenoids, taraxerone （2）, taraxerol （3）, 2α,3β,23,24-tetrahydroxyolean-12-en-28-oic acid （4）, oleanolic acid （5）, β-amyrin （6）, 3β,23-dihydroxylursan-12-en- 28-oic acid （7）, 2α,3β-dihydroxyursan-12-en-28-oic acid （8）, 2α,3β,23-trihydroxyursan-12-en-28-oic acid （9）, 2α,3β,24- trihydroxyursan-12-en-28-oic acid （10）, u rsolic acid （11）, and 3-O-acetylursolic acid （12）, was isolated from Craibiodendron henryi W. W. Smith （Ericaceae）. The structures of these compounds were elucidated on the basis of spectral evidence. Antioxidant activity and vasodilator effect of compound 1 were assessed.展开更多
目的阐明新月菱形藻胞外多糖(exopolysaccharides of Nitzschia closterium,EPN)的成分、单糖组成及其硫酸酯化条件。方法新月菱形藻胞外多糖以醇沉法从该藻的培养液中制备;DEAE-Sephedex25柱分离,HPLC法测定其质量分数,HPLC测定其相对...目的阐明新月菱形藻胞外多糖(exopolysaccharides of Nitzschia closterium,EPN)的成分、单糖组成及其硫酸酯化条件。方法新月菱形藻胞外多糖以醇沉法从该藻的培养液中制备;DEAE-Sephedex25柱分离,HPLC法测定其质量分数,HPLC测定其相对分子质量;以氯磺酸-吡啶法探索新月菱形藻硫酸酯化反应条件。结果新月菱形藻向胞外分泌3种多糖,相对分子质量分别为EPN11.886×105,EPN21.41×105,EPN31.134×105。3种多糖的单糖组成均为甘露糖、鼠李糖、葡萄糖醛酸、葡萄糖和岩藻糖。甘露糖、鼠李糖、葡萄糖醛酸、葡萄糖和岩藻糖的摩尔比在EPN1为4.3∶1∶5.1∶8.9∶5.6,EPN2为4.2∶7.3∶3.7∶12.8∶3.4,EPN3为3.5∶2.1∶4.3∶4.9∶4.7。3种多糖的硫酸酯化条件基本一致,提高氯磺酸比例和延长反应时间均有利于增加酯化产物的硫酸根、提高取代度和酸酯化反应的产率。结论新月菱形藻胞外多糖为酸性多糖,其硫酸酯化产物多糖硫酸酯可能具有潜在的药理活性。展开更多
基金Project(11JJ2042)supported by the Natural Science Foundation of Hunan Province,ChinaProject supported by the "Twelfth Five-Year" Key Discipline of Hunan University of Chinese Medicine-Pharmaceutical Analysis Science,China+1 种基金Project(11K048)supported by the Innovation Platform and Open Foundation Program of Higher Colleges of Hunan Province,ChinaProject(K1207010-21)supported by the Changsha City Science and Technology Bureau Key Projects,China
文摘To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax graeilistylus W. W. Smith （AGS）, the crystal structure was measured with a Bruker APEX-Ⅱ area-detector diffractometer instrument and the technology of extracting in combination hydrolysis in situ （ECHS） was compared with these of traditional methods. The crystal belongs to the monoclinic system, space group P2b with unit cell parameters： a=（8.3652±0.0006） nm, b=（24.721±0.002） nm, and c=（14.5587±0.0011） nm, α=90°, β=97.850 （4） °, γ=90 °, V=2982.51 nm3, Dc= 1.179 mg/m3, and the molecular number （Z） of elementary structures was 2. The comparisons show that the extraction rate of acankoreanogenin with ECHS methods is much higher than that of traditional methods. Then, central composite design-response surface methodology （CCD-RSM） was adopted for optimizing the extraction rate of ECHS methods. The optimized values of extraction parameters are as follows： for the for extraction process of acid hydrolysis are that extraction time 110.8 min, solvent-herb ratio 11.5 and acid content 5.25%; the best extraction process of basic hydrolysis are that extract time 120 min, solvent-herb ratio 8.7 and the alkali content 8.79%. Finally, the extracts were purified with decolorizing carbon after alkali solution and acid-isolation and purity of acankoreanogenin was 98.7%.
基金Supported by the National Natural Science Foundation of China(20432030 and 20672145)the State Key Basic Research and Development Plan of China(2004CB518906).
文摘A new triterpenoid, 11a.O.trans-p-coumaroyltaraxerol （1）, along with 11 known triterpenoids, taraxerone （2）, taraxerol （3）, 2α,3β,23,24-tetrahydroxyolean-12-en-28-oic acid （4）, oleanolic acid （5）, β-amyrin （6）, 3β,23-dihydroxylursan-12-en- 28-oic acid （7）, 2α,3β-dihydroxyursan-12-en-28-oic acid （8）, 2α,3β,23-trihydroxyursan-12-en-28-oic acid （9）, 2α,3β,24- trihydroxyursan-12-en-28-oic acid （10）, u rsolic acid （11）, and 3-O-acetylursolic acid （12）, was isolated from Craibiodendron henryi W. W. Smith （Ericaceae）. The structures of these compounds were elucidated on the basis of spectral evidence. Antioxidant activity and vasodilator effect of compound 1 were assessed.
文摘目的阐明新月菱形藻胞外多糖(exopolysaccharides of Nitzschia closterium,EPN)的成分、单糖组成及其硫酸酯化条件。方法新月菱形藻胞外多糖以醇沉法从该藻的培养液中制备;DEAE-Sephedex25柱分离,HPLC法测定其质量分数,HPLC测定其相对分子质量;以氯磺酸-吡啶法探索新月菱形藻硫酸酯化反应条件。结果新月菱形藻向胞外分泌3种多糖,相对分子质量分别为EPN11.886×105,EPN21.41×105,EPN31.134×105。3种多糖的单糖组成均为甘露糖、鼠李糖、葡萄糖醛酸、葡萄糖和岩藻糖。甘露糖、鼠李糖、葡萄糖醛酸、葡萄糖和岩藻糖的摩尔比在EPN1为4.3∶1∶5.1∶8.9∶5.6,EPN2为4.2∶7.3∶3.7∶12.8∶3.4,EPN3为3.5∶2.1∶4.3∶4.9∶4.7。3种多糖的硫酸酯化条件基本一致,提高氯磺酸比例和延长反应时间均有利于增加酯化产物的硫酸根、提高取代度和酸酯化反应的产率。结论新月菱形藻胞外多糖为酸性多糖,其硫酸酯化产物多糖硫酸酯可能具有潜在的药理活性。
