Objective Silicosis, caused by inhalation of silica dust, is the most serious occupational disease in China and the aim of present study was to explore the protective effect of Ang(1-7) on silicotic fibrosis and myofi...Objective Silicosis, caused by inhalation of silica dust, is the most serious occupational disease in China and the aim of present study was to explore the protective effect of Ang(1-7) on silicotic fibrosis and myofibroblast differentiation induced by Ang II. Methods HOPE-MED 8050 exposure control apparatus was used to establish the rat silicosis model. Pathological changes and collagen deposition of the lung tissue were examined by H.E. and VG staining, respectively. The localizations of ACE2 and α-smooth muscle actin(α-SMA) in the lung were detected by immunohistochemistry. Expression levels of collagen type I, α-SMA, ACE2, and Mas in the lung tissue and fibroblasts were examined by western blot. Levels of ACE2, Ang(1-7), and Ang II in serum were determined by ELISA. Co-localization of ACE2 and α-SMA in fibroblasts was detected by immunofluorescence. Results Ang(1-7) induced pathological changes and enhanced collagen deposition in vivo. Ang(1-7) decreased the expressions of collagen type I and α-SMA and increased the expressions of ACE2 and Mas in the silicotic rat lung tissue and fibroblasts stimulated by Ang II. Ang(1-7) increased the levels of ACE2 and Ang(1-7) and decreased the level of Ang II in silicotic rat serum. A779 enhanced the protective effect of Ang(1-7) in fibroblasts stimulated by Ang II. Conclusion Ang(1-7) exerted protective effect on silicotic fibrosis and myofibroblast differentiation induced by Ang II by regulating ACE2-Ang(1-7)-Mas axis.展开更多
Objectives： Ocular effects of Mas-receptor ligands were studied in an experimental rat glaucoma. Elevated IOP （intraocular pressure） was induced unilaterally by laser photocoagulation of the episcleral and limbal v...Objectives： Ocular effects of Mas-receptor ligands were studied in an experimental rat glaucoma. Elevated IOP （intraocular pressure） was induced unilaterally by laser photocoagulation of the episcleral and limbal veins in anesthetized rats. A Mas-receptor agonist （Ang （1-7）） and an antagonist （A779） were administered intravitreally in the glaucomatous eye. lOP was measured by a rebound tonometer. Effects of the treatment on RGCL （retinal ganglion cell layer） were determined stereologically and on the axons of optic nerve by a modified Gallyas silver-staining method. Key findings： Mean IOP during the 14 days follow-up in the solvent treated glaucoma eyes （n = 18） was 28.7 -4- 1.9 mmHg vs. the fellow eyes 11.0 4- 0.3 mmHg. A significant axon damage was detected in the glaucomatous eyes vs. the fellow normotensive eye. The Mas-receptor ligands did not influence high IOP resulted by laser treatment, Despite of the ineffectiveness on lOP, Ang （1-7） protected RGCL cells as determined by stereology （P = 0.016）. No significant effects in Gallyas silver-staining were found. Summary： Intravitreally administered Ang （1-7） showed a significant protective effect against neuronal damage. The present and our previous studies suggest that stimulation of Mas-receptor may have therapeutic potential to treat glaucoma.展开更多
基金supported by the National Natural Science Foundation of China [grant no.81472953]Natural Science Foundation of Hebei Province [grant no.H2016209170]+2 种基金Graduate Student Innovation Fund of Hebei Province [grant no.2016196]Graduate Student Innovation Fund of North China University of Science and Technology [grant no.2016B10]undergraduate innovative project of North China University of Science and Technology [grant no.X2017354]
文摘Objective Silicosis, caused by inhalation of silica dust, is the most serious occupational disease in China and the aim of present study was to explore the protective effect of Ang(1-7) on silicotic fibrosis and myofibroblast differentiation induced by Ang II. Methods HOPE-MED 8050 exposure control apparatus was used to establish the rat silicosis model. Pathological changes and collagen deposition of the lung tissue were examined by H.E. and VG staining, respectively. The localizations of ACE2 and α-smooth muscle actin(α-SMA) in the lung were detected by immunohistochemistry. Expression levels of collagen type I, α-SMA, ACE2, and Mas in the lung tissue and fibroblasts were examined by western blot. Levels of ACE2, Ang(1-7), and Ang II in serum were determined by ELISA. Co-localization of ACE2 and α-SMA in fibroblasts was detected by immunofluorescence. Results Ang(1-7) induced pathological changes and enhanced collagen deposition in vivo. Ang(1-7) decreased the expressions of collagen type I and α-SMA and increased the expressions of ACE2 and Mas in the silicotic rat lung tissue and fibroblasts stimulated by Ang II. Ang(1-7) increased the levels of ACE2 and Ang(1-7) and decreased the level of Ang II in silicotic rat serum. A779 enhanced the protective effect of Ang(1-7) in fibroblasts stimulated by Ang II. Conclusion Ang(1-7) exerted protective effect on silicotic fibrosis and myofibroblast differentiation induced by Ang II by regulating ACE2-Ang(1-7)-Mas axis.
文摘Objectives： Ocular effects of Mas-receptor ligands were studied in an experimental rat glaucoma. Elevated IOP （intraocular pressure） was induced unilaterally by laser photocoagulation of the episcleral and limbal veins in anesthetized rats. A Mas-receptor agonist （Ang （1-7）） and an antagonist （A779） were administered intravitreally in the glaucomatous eye. lOP was measured by a rebound tonometer. Effects of the treatment on RGCL （retinal ganglion cell layer） were determined stereologically and on the axons of optic nerve by a modified Gallyas silver-staining method. Key findings： Mean IOP during the 14 days follow-up in the solvent treated glaucoma eyes （n = 18） was 28.7 -4- 1.9 mmHg vs. the fellow eyes 11.0 4- 0.3 mmHg. A significant axon damage was detected in the glaucomatous eyes vs. the fellow normotensive eye. The Mas-receptor ligands did not influence high IOP resulted by laser treatment, Despite of the ineffectiveness on lOP, Ang （1-7） protected RGCL cells as determined by stereology （P = 0.016）. No significant effects in Gallyas silver-staining were found. Summary： Intravitreally administered Ang （1-7） showed a significant protective effect against neuronal damage. The present and our previous studies suggest that stimulation of Mas-receptor may have therapeutic potential to treat glaucoma.
