[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone ...[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana; then the amplified sequence was sequenced and homologically analyzed. [Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ) approaches. The full length of this cDNA was 866 bp encoding 197 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene (Ma-14-3-3d). Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species. [Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon.展开更多
先进绝热压缩空气储能(advanced adiabatic compressed air energy storage,AA-CAES)装置因具有大容量、寿命长、污染少、成本低等优点而日渐受到关注,其大容量多能联供联储特点与建筑能耗特征相匹配,接入由多建筑微网构成的区域综合能...先进绝热压缩空气储能(advanced adiabatic compressed air energy storage,AA-CAES)装置因具有大容量、寿命长、污染少、成本低等优点而日渐受到关注,其大容量多能联供联储特点与建筑能耗特征相匹配,接入由多建筑微网构成的区域综合能源系统(regional integrated energy system,RIES),两者相得益彰。文章建立了一种含AA-CAES的由多个建筑微网组成的区域综合能源系统模型,考虑压缩空气储能设备储气、储热双荷能状态以建立季节性效率优化模型,对AA-CAES的运行模式进行季节性优化,在区域内多能流平衡约束分析的基础上构建关于系统综合效益最优的二次约束问题,利用Gurobi求解器求解。仿真表明,改进的AA-CAES可协调区域内多个建筑微网的多能优化,提高系统经济性、节能性、环保性,促进可再生能源就地消纳与能源梯级利用。展开更多
文摘[Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana. [Method] Combined with PCR amplification, RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana; then the amplified sequence was sequenced and homologically analyzed. [Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ) approaches. The full length of this cDNA was 866 bp encoding 197 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene (Ma-14-3-3d). Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species. [Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon.
文摘先进绝热压缩空气储能(advanced adiabatic compressed air energy storage,AA-CAES)装置因具有大容量、寿命长、污染少、成本低等优点而日渐受到关注,其大容量多能联供联储特点与建筑能耗特征相匹配,接入由多建筑微网构成的区域综合能源系统(regional integrated energy system,RIES),两者相得益彰。文章建立了一种含AA-CAES的由多个建筑微网组成的区域综合能源系统模型,考虑压缩空气储能设备储气、储热双荷能状态以建立季节性效率优化模型,对AA-CAES的运行模式进行季节性优化,在区域内多能流平衡约束分析的基础上构建关于系统综合效益最优的二次约束问题,利用Gurobi求解器求解。仿真表明,改进的AA-CAES可协调区域内多个建筑微网的多能优化,提高系统经济性、节能性、环保性,促进可再生能源就地消纳与能源梯级利用。
