Biobutanol is an advanced biofuel that can be produced from excess lignocellulose via acetone-butanol-ethanol(ABE)fermentation.Although significant technological progress has been made in this field,attempts at larges...Biobutanol is an advanced biofuel that can be produced from excess lignocellulose via acetone-butanol-ethanol(ABE)fermentation.Although significant technological progress has been made in this field,attempts at largescale lignocellulosic ABE production remain scarce.In this study,1m^(3)scale ABE fermentation was investigated using high inhibitor tolerance Clostridium acetobutylicum ABE-P1201 and steam-exploded corn stover hydrolysate(SECSH).Before expanding the fermentation scale,the detoxification process for SECSH was simplified by process engineering.Results revealed that appropriate pH management during the fed-batch cultivation could largely decrease the inhibition of the toxic components in undetoxified SECSH to the solventogenesis phase of the ABE-P1201 strains,avoiding“acid crash”.Therefore,after naturalizing the pH by Ca(OH)_(2),the undetoxified SECSH,without removal of the solid components,reached 17.68±1.30 g/L of ABE production with 0.34±0.01 g/g of yield in 1 L scale bioreactor.Based on this strategy,the fermentation scale gradually expanded from laboratory-scale apparatus to pilot-scale bioreactors.Finally,17.05±1.20 g/L of ABE titer and 0.32±0.01 g/g of ABE yield were realized in 1m3 bioreactor,corresponding to approximately 145 kg of ABE production from 1 t of dry corn stover.The pilot-scale ABE fermentation demonstrated excellent stability during repeated operations.This study provided a simplified ABE fermentation strategy and verified the feasibility of the pilot process,providing tremendous significance and a solid foundation for the future industrialization of second-generation ABE plants.展开更多
Performance of continuous 1-butanol production process by Clostridium bacteria is complicated by alternation of acidogenic and solventogenic phases during fermentation what correlates with culture growth and sporulati...Performance of continuous 1-butanol production process by Clostridium bacteria is complicated by alternation of acidogenic and solventogenic phases during fermentation what correlates with culture growth and sporulation, respectively. However, the strain Clostridium pasteurianum NRRL B598 showed diverse pattern of solvents production when butanol production started during exponential growth phase in batch cultivation. If cultivated under glucose limited conditions with dilution rate 0.03 hl, constant product concentration was almost achieved. By contrast, glucose non-limited continuous experiment operated with dilution rate 0.07 h-1 lead to mutually adverse oscillations of product and glucose concentrations. Regarding the process economy, glucose limited continuous cultivation seems to be superior due to utilization of total supplied glucose amount, advantageous butanol/acetone ratio and higher butanol yield.展开更多
基金funded by the National Key Research and Development Program of China(grant number:2022YFC2106300).
文摘Biobutanol is an advanced biofuel that can be produced from excess lignocellulose via acetone-butanol-ethanol(ABE)fermentation.Although significant technological progress has been made in this field,attempts at largescale lignocellulosic ABE production remain scarce.In this study,1m^(3)scale ABE fermentation was investigated using high inhibitor tolerance Clostridium acetobutylicum ABE-P1201 and steam-exploded corn stover hydrolysate(SECSH).Before expanding the fermentation scale,the detoxification process for SECSH was simplified by process engineering.Results revealed that appropriate pH management during the fed-batch cultivation could largely decrease the inhibition of the toxic components in undetoxified SECSH to the solventogenesis phase of the ABE-P1201 strains,avoiding“acid crash”.Therefore,after naturalizing the pH by Ca(OH)_(2),the undetoxified SECSH,without removal of the solid components,reached 17.68±1.30 g/L of ABE production with 0.34±0.01 g/g of yield in 1 L scale bioreactor.Based on this strategy,the fermentation scale gradually expanded from laboratory-scale apparatus to pilot-scale bioreactors.Finally,17.05±1.20 g/L of ABE titer and 0.32±0.01 g/g of ABE yield were realized in 1m3 bioreactor,corresponding to approximately 145 kg of ABE production from 1 t of dry corn stover.The pilot-scale ABE fermentation demonstrated excellent stability during repeated operations.This study provided a simplified ABE fermentation strategy and verified the feasibility of the pilot process,providing tremendous significance and a solid foundation for the future industrialization of second-generation ABE plants.
文摘Performance of continuous 1-butanol production process by Clostridium bacteria is complicated by alternation of acidogenic and solventogenic phases during fermentation what correlates with culture growth and sporulation, respectively. However, the strain Clostridium pasteurianum NRRL B598 showed diverse pattern of solvents production when butanol production started during exponential growth phase in batch cultivation. If cultivated under glucose limited conditions with dilution rate 0.03 hl, constant product concentration was almost achieved. By contrast, glucose non-limited continuous experiment operated with dilution rate 0.07 h-1 lead to mutually adverse oscillations of product and glucose concentrations. Regarding the process economy, glucose limited continuous cultivation seems to be superior due to utilization of total supplied glucose amount, advantageous butanol/acetone ratio and higher butanol yield.
