The effects of mepiquat chloride(DPC)on the Cry1Ac protein content in Bacillus thuringiensis(Bt)cotton boll shells under high temperature and drought stress were investigated to provide a theoretical reference for Bt ...The effects of mepiquat chloride(DPC)on the Cry1Ac protein content in Bacillus thuringiensis(Bt)cotton boll shells under high temperature and drought stress were investigated to provide a theoretical reference for Bt cotton breeding and high-yield and-efficiency cotton cultivation.This study was conducted using Bt cotton cultivar‘Sikang 3'during the 2020 and 2021 growing seasons at Yangzhou University Farm,Yangzhou,Jiangsu Province,China.Potted cotton plants were exposed to high temperature and drought stress,and sprayed with either 20 mg L^(-1)DPC or water(CK).Seven days after treatment,the Cry1Ac protein content,α-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content were measured,and transcriptome sequencing was performed.DESeq was used for differential gene analysis.Under the DPC treatment,the Cry1Ac protein content increased by 4.7-11.9% compared to CK.Theα-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content all increased.Transcriptome analysis revealed 7,542 upregulated genes and 10,449 downregulated genes for DPC vs.CK.Gene ontology(GO)and Kyoto Encyclopedia of Gene and Genomes(KEGG)analyses showed that the differentially expressed genes were mainly involved in biological processes,such as carbon and amino acid metabolism.For example,genes encoding 6-phosphofructokinase,pyruvate kinase,glutamic pyruvate transaminase,pyruvate dehydrogenase,citrate synthase,isocitrate dehydrogenase,2-oxoglutarate dehydrogenase,glutamate synthase,1-pyrroline-5-carboxylate dehydrogenase,glutamic oxaloacetic transaminase,amino-acid N-acetyltransferase,and acetylornithine deacetylase were all significantly upregulated.The DPC treatment increased pyruvate,α-ketoglutarate,and oxaloacetate by increasing the operational rate of the glycolytic pathway of the citric acid cycle.It also significantly upregulated the genes encoding glutamate synthase,pyrrolidine-5-carboxylic acid dehydrogenase,glutamate oxaloacetate transaminase,and N-acetylglutamate synthetase,while it downregulated the genes encoding glutamine synthetase.Therefore,the synthesis of aspartic acid,glutamic acid,pyruvate,and arginine increased after treatment with DPC,and the Cry1Ac protein content was increased by regulating carbon and amino acid metabolism.展开更多
Background Bt technology has played significant role in controlling bollworms and increasing cotton yield in earlier days of its introduction,a subsequent decline in yield became apparent over time.This decline may be...Background Bt technology has played significant role in controlling bollworms and increasing cotton yield in earlier days of its introduction,a subsequent decline in yield became apparent over time.This decline may be attributed to various environmental factors,pest dynamics,or combination of both.Therefore,the present biophysical survey and questionnaire were designed to evaluate the impact of Bt cotton on bollworms management and its effect on reducing spray costs,targeting farmers with varied landholdings and educational backgrounds.Additionally,data on farmers'cultivated varieties and the prevalence of bollworms and sucking insects in their fields were recorded.Subsequently,about eleven thousand cotton samples from farmer fields were tested for Cry1Ac,Cry2Ab and Vip3A genes by strip test.Results In this analysis,83% of the farmers planting approved varieties believe that Bt technology control bollworms,while 17% hold contradictory views.Similarly,among farmers cultivating unapproved varieties,77% agree on effectiveness of Bt technology against bollworms,while 23% disagree.On the other hand,67% of farmers planting approved varieties believe that Bt technology does not reduce spray costs,while 33% agree with the effectiveness.Similarly,78% of farmers cultivating unapproved varieties express doubt regarding its role to reduce spray costs,while 22% are in favour of this notion.Differences in opinions on the effectiveness of Bt cotton in controlling bollworms and reducing spray cost between farmers planting unapproved and approved varieties may stem from several factors.One major cause is the heavy infestation of sucking insects,which is probably due to the narrow genetic variation of the cultivated varieties.Additionally,the widespread cultivation of unapproved varieties(21.67%)is also an important factor to cause different opinions on the effectiveness of Bt cotton.Conclusion Based on our findings,we propose that the ineffective control of pests on cotton crop may be attributed to large scale cultivation of unapproved varieties and non-inclusion of double and triple transgene technologies in country's sowing plan.On the basis of our findings,we suggest cotton breeders,regulatory bodies and legislative bodies to discourage the cultivation of unapproved varieties and impure seed.Moreover,the adoption of double and triple Bt genes in cottons with a broad genetic variation could facilitate the revival of the cotton industry,and presenting a promising way forward.展开更多
基金supported by the National Natural Science Foundation of China(31901462)the Natural Science Foundation of the Jiangsu Higher Education Institutions,China(22KJA210005)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD)the Brand Professional Construction Program of Jiangsu Higher Education Institutions,China。
文摘The effects of mepiquat chloride(DPC)on the Cry1Ac protein content in Bacillus thuringiensis(Bt)cotton boll shells under high temperature and drought stress were investigated to provide a theoretical reference for Bt cotton breeding and high-yield and-efficiency cotton cultivation.This study was conducted using Bt cotton cultivar‘Sikang 3'during the 2020 and 2021 growing seasons at Yangzhou University Farm,Yangzhou,Jiangsu Province,China.Potted cotton plants were exposed to high temperature and drought stress,and sprayed with either 20 mg L^(-1)DPC or water(CK).Seven days after treatment,the Cry1Ac protein content,α-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content were measured,and transcriptome sequencing was performed.DESeq was used for differential gene analysis.Under the DPC treatment,the Cry1Ac protein content increased by 4.7-11.9% compared to CK.Theα-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content all increased.Transcriptome analysis revealed 7,542 upregulated genes and 10,449 downregulated genes for DPC vs.CK.Gene ontology(GO)and Kyoto Encyclopedia of Gene and Genomes(KEGG)analyses showed that the differentially expressed genes were mainly involved in biological processes,such as carbon and amino acid metabolism.For example,genes encoding 6-phosphofructokinase,pyruvate kinase,glutamic pyruvate transaminase,pyruvate dehydrogenase,citrate synthase,isocitrate dehydrogenase,2-oxoglutarate dehydrogenase,glutamate synthase,1-pyrroline-5-carboxylate dehydrogenase,glutamic oxaloacetic transaminase,amino-acid N-acetyltransferase,and acetylornithine deacetylase were all significantly upregulated.The DPC treatment increased pyruvate,α-ketoglutarate,and oxaloacetate by increasing the operational rate of the glycolytic pathway of the citric acid cycle.It also significantly upregulated the genes encoding glutamate synthase,pyrrolidine-5-carboxylic acid dehydrogenase,glutamate oxaloacetate transaminase,and N-acetylglutamate synthetase,while it downregulated the genes encoding glutamine synthetase.Therefore,the synthesis of aspartic acid,glutamic acid,pyruvate,and arginine increased after treatment with DPC,and the Cry1Ac protein content was increased by regulating carbon and amino acid metabolism.
基金supported by Punjab Agriculture Research Board,Grant numbers PARB No.890received research support from Punjab Agriculture Board。
文摘Background Bt technology has played significant role in controlling bollworms and increasing cotton yield in earlier days of its introduction,a subsequent decline in yield became apparent over time.This decline may be attributed to various environmental factors,pest dynamics,or combination of both.Therefore,the present biophysical survey and questionnaire were designed to evaluate the impact of Bt cotton on bollworms management and its effect on reducing spray costs,targeting farmers with varied landholdings and educational backgrounds.Additionally,data on farmers'cultivated varieties and the prevalence of bollworms and sucking insects in their fields were recorded.Subsequently,about eleven thousand cotton samples from farmer fields were tested for Cry1Ac,Cry2Ab and Vip3A genes by strip test.Results In this analysis,83% of the farmers planting approved varieties believe that Bt technology control bollworms,while 17% hold contradictory views.Similarly,among farmers cultivating unapproved varieties,77% agree on effectiveness of Bt technology against bollworms,while 23% disagree.On the other hand,67% of farmers planting approved varieties believe that Bt technology does not reduce spray costs,while 33% agree with the effectiveness.Similarly,78% of farmers cultivating unapproved varieties express doubt regarding its role to reduce spray costs,while 22% are in favour of this notion.Differences in opinions on the effectiveness of Bt cotton in controlling bollworms and reducing spray cost between farmers planting unapproved and approved varieties may stem from several factors.One major cause is the heavy infestation of sucking insects,which is probably due to the narrow genetic variation of the cultivated varieties.Additionally,the widespread cultivation of unapproved varieties(21.67%)is also an important factor to cause different opinions on the effectiveness of Bt cotton.Conclusion Based on our findings,we propose that the ineffective control of pests on cotton crop may be attributed to large scale cultivation of unapproved varieties and non-inclusion of double and triple transgene technologies in country's sowing plan.On the basis of our findings,we suggest cotton breeders,regulatory bodies and legislative bodies to discourage the cultivation of unapproved varieties and impure seed.Moreover,the adoption of double and triple Bt genes in cottons with a broad genetic variation could facilitate the revival of the cotton industry,and presenting a promising way forward.
