目的:探讨去整合素金属蛋白酶9(a disintegrin and metalloproteinase 9,ADAM9)蛋白在人乳腺癌细胞系中的表达。方法:提取人正常乳腺上皮细胞HBL100,人乳腺癌细胞系MCF-7,ZR-75-1,MDA-MB-231,HCC1937和Hs578T,小鼠乳腺癌细胞系4T1的总...目的:探讨去整合素金属蛋白酶9(a disintegrin and metalloproteinase 9,ADAM9)蛋白在人乳腺癌细胞系中的表达。方法:提取人正常乳腺上皮细胞HBL100,人乳腺癌细胞系MCF-7,ZR-75-1,MDA-MB-231,HCC1937和Hs578T,小鼠乳腺癌细胞系4T1的总蛋白和总mRNA,提取细胞的总蛋白和总RNA进行蛋白免疫印迹和实时定量PCR检测ADAM9的表达。结果:在人乳腺癌细胞系MDA-MB-231中ADAM9蛋白表达最高,在小鼠乳腺癌4T1细胞中ADAM9表达较低。结论:ADAM9在高侵袭的MDA-MB-231乳腺癌细胞中高表达。展开更多
去整合素–金属蛋白酶家族(adisintegrin and metalloproteinase, ADAMs)是一类锌依赖的跨膜糖蛋白,ADAMs家族分子具有相似的分子结构包括:结构域(单肽)末端金属域、整合素域、富含半胱氨酸的序列、膜近端上皮生长因子样序列。ADAM9在...去整合素–金属蛋白酶家族(adisintegrin and metalloproteinase, ADAMs)是一类锌依赖的跨膜糖蛋白,ADAMs家族分子具有相似的分子结构包括:结构域(单肽)末端金属域、整合素域、富含半胱氨酸的序列、膜近端上皮生长因子样序列。ADAM9在胶质瘤、黑色素瘤、前列腺癌、胰腺导管腺癌、胃癌、乳腺癌、肺癌和肝癌等实体肿瘤中均有过表达。免疫组织化学分析强调了ADAM9在实际癌细胞中的表达,并将其丰富的表达与临床病理特征联系起来,如总体生存期缩短、肿瘤分级较差、去分化、治疗耐药性和转移形成。本文综述了ADAM9在肿瘤中的研究现状,以及它在推动肿瘤进展方面的不同机制。展开更多
The 'a disintegrin and metalloprotease' (ADAM) family contributes to regulation of the cell-cell and cell-matrix interactions that are critical determinants of malignancy. To determine the relationship between...The 'a disintegrin and metalloprotease' (ADAM) family contributes to regulation of the cell-cell and cell-matrix interactions that are critical determinants of malignancy. To determine the relationship between metastasis and ADAM proteins, we compared the mRNA levels of ADAM9,-10,-12,-15, and-17 in sublines of an EBC-1 lung cancer cell line that were highly metastatic to either brain or bone. ADAM9 mRNA levels were significantly higher in highly brain-metastatic sublines than in the parent or highly bonemetastalic sublines. To elucidate the role ofADAM9 in brain metastasis, we stably transfected A549 and EBC-1 cells with a fulllength ADAM9 expression vector. Compared with mock-transfectants, ADAM9 overexpression resulted in increased invasive capacity展开更多
Proteases are important molecules that are involved in many physiological and pathological processes of the human body,such as growth,apoptosis and metastasis cancer cells.They are potential targets in cancer diagnosi...Proteases are important molecules that are involved in many physiological and pathological processes of the human body,such as growth,apoptosis and metastasis cancer cells.They are potential targets in cancer diagnosis and biotherapy.In this study,we analyzed the salivary protease spectrum of patients with oral squamous cell carcinoma (OSCC),oral benign masses and chronic periodontitis,as well as that of health,using human protease array kits,enzyme-linked immunosorbent assay,western blot and immunofluorescence.The salivary protease spectrum was found to be associated with oral diseases.For example,the saliva of patients with OSCC contained increased numbers of proteases than those of other oral diseases and health.The levels of matrix metalloproteinase (MMP)-1,MMP-2,MMP-10,MMP-12,A disintegrin and metalloprotease (ADAM)9,A disintegrin and metalloprotease with thrombospondin type 13 motifs (ADAMST13),cathepsin V and kallikrein 5 in the saliva of patients with OSCC were significantly increased compared with those of other groups.Taking MMP-1,cathepsin V,kallikrein 5 and ADAM9 as biomarkers of OSCC,cutoff values were199,11.34,9.29 and 202.55 pg·mL?1,respectively.From the area under the curve,sensitivity and specificity,the combination of cathepsin V/kallikrein5/ADAM9 was an optimal biomarker for diagnosing OSCC.Thus,analysis of the salivary protease spectrum may be an innovative and cost-efficient approach to evaluating the health status of the oral cavity.Specifically,increases in cathepsin V,kallikrein 5 and ADAM9 may be useful biomarkers in the screening and diagnosis of OSCC.展开更多
目的寻找三阴性乳腺癌(TNBC)研究与治疗的可能靶点,并采用生物信息学对影响TNBC预后的基因进行分析预测。方法检索The National Center for Biotechnology Information(NCBI)数据库,分析不同组间差异基因后提交至Enrichr网站进行通路富...目的寻找三阴性乳腺癌(TNBC)研究与治疗的可能靶点,并采用生物信息学对影响TNBC预后的基因进行分析预测。方法检索The National Center for Biotechnology Information(NCBI)数据库,分析不同组间差异基因后提交至Enrichr网站进行通路富集,最后进行TNBC患者的生存分析。结果去整合素金属蛋白酶9(ADAM9)基因与TNBC患者的预后不良有相关性(P<0.05),且其仅与TNBC患者预后有关,与其他分型乳腺癌无关。结论ADAM9基因被证明与TNBC患者的不良预后有关,可将其视为导致TNBC患者淋巴结转移,最终预后不良的可能关键基因。展开更多
文摘目的:探讨去整合素金属蛋白酶9(a disintegrin and metalloproteinase 9,ADAM9)蛋白在人乳腺癌细胞系中的表达。方法:提取人正常乳腺上皮细胞HBL100,人乳腺癌细胞系MCF-7,ZR-75-1,MDA-MB-231,HCC1937和Hs578T,小鼠乳腺癌细胞系4T1的总蛋白和总mRNA,提取细胞的总蛋白和总RNA进行蛋白免疫印迹和实时定量PCR检测ADAM9的表达。结果:在人乳腺癌细胞系MDA-MB-231中ADAM9蛋白表达最高,在小鼠乳腺癌4T1细胞中ADAM9表达较低。结论:ADAM9在高侵袭的MDA-MB-231乳腺癌细胞中高表达。
文摘去整合素–金属蛋白酶家族(adisintegrin and metalloproteinase, ADAMs)是一类锌依赖的跨膜糖蛋白,ADAMs家族分子具有相似的分子结构包括:结构域(单肽)末端金属域、整合素域、富含半胱氨酸的序列、膜近端上皮生长因子样序列。ADAM9在胶质瘤、黑色素瘤、前列腺癌、胰腺导管腺癌、胃癌、乳腺癌、肺癌和肝癌等实体肿瘤中均有过表达。免疫组织化学分析强调了ADAM9在实际癌细胞中的表达,并将其丰富的表达与临床病理特征联系起来,如总体生存期缩短、肿瘤分级较差、去分化、治疗耐药性和转移形成。本文综述了ADAM9在肿瘤中的研究现状,以及它在推动肿瘤进展方面的不同机制。
文摘The 'a disintegrin and metalloprotease' (ADAM) family contributes to regulation of the cell-cell and cell-matrix interactions that are critical determinants of malignancy. To determine the relationship between metastasis and ADAM proteins, we compared the mRNA levels of ADAM9,-10,-12,-15, and-17 in sublines of an EBC-1 lung cancer cell line that were highly metastatic to either brain or bone. ADAM9 mRNA levels were significantly higher in highly brain-metastatic sublines than in the parent or highly bonemetastalic sublines. To elucidate the role ofADAM9 in brain metastasis, we stably transfected A549 and EBC-1 cells with a fulllength ADAM9 expression vector. Compared with mock-transfectants, ADAM9 overexpression resulted in increased invasive capacity
基金the National Natural Science Foundation of China (No. 81372892,81621062, 81520108009)111 Project of MOE China (B14038)+1 种基金the Open Foundation (SKLOD201601, SKLOD2016OF01) from the State Key Laboratory of Oral Disease Sichuan University and for the financial supportSichuan Province Science and Technology Innovation Team Program (JCPT 2011–9)
文摘Proteases are important molecules that are involved in many physiological and pathological processes of the human body,such as growth,apoptosis and metastasis cancer cells.They are potential targets in cancer diagnosis and biotherapy.In this study,we analyzed the salivary protease spectrum of patients with oral squamous cell carcinoma (OSCC),oral benign masses and chronic periodontitis,as well as that of health,using human protease array kits,enzyme-linked immunosorbent assay,western blot and immunofluorescence.The salivary protease spectrum was found to be associated with oral diseases.For example,the saliva of patients with OSCC contained increased numbers of proteases than those of other oral diseases and health.The levels of matrix metalloproteinase (MMP)-1,MMP-2,MMP-10,MMP-12,A disintegrin and metalloprotease (ADAM)9,A disintegrin and metalloprotease with thrombospondin type 13 motifs (ADAMST13),cathepsin V and kallikrein 5 in the saliva of patients with OSCC were significantly increased compared with those of other groups.Taking MMP-1,cathepsin V,kallikrein 5 and ADAM9 as biomarkers of OSCC,cutoff values were199,11.34,9.29 and 202.55 pg·mL?1,respectively.From the area under the curve,sensitivity and specificity,the combination of cathepsin V/kallikrein5/ADAM9 was an optimal biomarker for diagnosing OSCC.Thus,analysis of the salivary protease spectrum may be an innovative and cost-efficient approach to evaluating the health status of the oral cavity.Specifically,increases in cathepsin V,kallikrein 5 and ADAM9 may be useful biomarkers in the screening and diagnosis of OSCC.
文摘目的寻找三阴性乳腺癌(TNBC)研究与治疗的可能靶点,并采用生物信息学对影响TNBC预后的基因进行分析预测。方法检索The National Center for Biotechnology Information(NCBI)数据库,分析不同组间差异基因后提交至Enrichr网站进行通路富集,最后进行TNBC患者的生存分析。结果去整合素金属蛋白酶9(ADAM9)基因与TNBC患者的预后不良有相关性(P<0.05),且其仅与TNBC患者预后有关,与其他分型乳腺癌无关。结论ADAM9基因被证明与TNBC患者的不良预后有关,可将其视为导致TNBC患者淋巴结转移,最终预后不良的可能关键基因。
