去整合素基质金属蛋白酶19(a disintegrin and metalloproteinase 19,ADAM19)是新近发现的ADAMs(a disintegrin and metalloproteinase)家族新成员,在人胎盘组织中有较高水平的表达,但其在胎盘发生过程中母胎界面的时空表达及功能还鲜...去整合素基质金属蛋白酶19(a disintegrin and metalloproteinase 19,ADAM19)是新近发现的ADAMs(a disintegrin and metalloproteinase)家族新成员,在人胎盘组织中有较高水平的表达,但其在胎盘发生过程中母胎界面的时空表达及功能还鲜有报道.本研究首次就ADAM19在正常胎盘中的时空表达进行了研究,并以人绒毛膜癌细胞系JEG-3细胞为体外研究模型分析了ADAM19对滋养层细胞侵润和黏附的影响及其机理.研究结果显示,从空间上看,ADAM19在多种滋养层细胞中有广泛的分布,包括细胞滋养层细胞(vctb)、合体滋养层细胞(stb)和滋养层细胞柱(ct),绒毛内毛细血管的内皮细胞等.RT-PCR和Western印迹分析显示在孕早期8,9周龄绒毛中ADAM19的表达量较高,但在26周和足月胎盘中其mRNA和蛋白水平均明显下调.在JEG-3细胞中瞬时转染ADAM19可以降低其侵润能力同时增强细胞间的黏附.研究结果提示,ADAM19可能是胎盘发生过程中非常重要的滋养层细胞功能的调节分子.展开更多
Human ADAM19 is a recently identified member of the ADAM family.It is highly expressed in human placentas,but its dynamic change and function at the human feto-maternal interface during placentation remain to be eluci...Human ADAM19 is a recently identified member of the ADAM family.It is highly expressed in human placentas,but its dynamic change and function at the human feto-maternal interface during placentation remain to be elucidated.In this present study,the spatial and temporal expression and cellular localization of ADAM19 in normal human placentas were first demonstrated,and the effects of ADAM19 on trophoblast cell adhesion and invasion were further investigated by using a human choriocarcinoma cell line(JEG-3) as an in vitro model.The data demonstrated that ADAM19 was widely distributed in villous cytotrophoblast cells,syncytiotrophoblast cells,column trophoblasts,and villous capillary endothelial cells during early pregnancy.The mRNA and protein level of ADAM19 in placentas was high at gestational weeks 8-9,but diminished significantly at mid-and term pregnancy.In JEG-3 cells,the overexpression of ADAM19 led to diminished cell invasion,as well as increases in cell adhesiveness and the expression of E-cadherin,with no changes in β-catenin expression observed.These data indicate that ADAM19 may participate in the coordinated regulation of human trophoblast cell behaviors during the process of placentation.展开更多
目的探讨解聚素-金属蛋白酶19水平与神经胶质瘤诊断、预后的相关性。方法收集初诊为原发神经胶质瘤的患者49例,回顾性分析胶质瘤ADAM-19的表达与临床因素的相关性,并结合远期随访结果对胶质瘤ADAM-19的表达水平与生存时间进行比较。结果...目的探讨解聚素-金属蛋白酶19水平与神经胶质瘤诊断、预后的相关性。方法收集初诊为原发神经胶质瘤的患者49例,回顾性分析胶质瘤ADAM-19的表达与临床因素的相关性,并结合远期随访结果对胶质瘤ADAM-19的表达水平与生存时间进行比较。结果 ADAM19的相对表达率在各组间的表达从小到大排列为对照组(0.57±0.13)<胶质瘤Ⅰ~Ⅱ级(1.24±0.46)<胶质瘤Ⅲ~Ⅳ级(3.78±0.85)。胶质瘤分型Ⅰ~Ⅱ组,ADAM-19低表达人数及所占比例明显高于高表达人数及比例(17/20 vs 3/20);相反的趋势可见于WHO分型Ⅲ~Ⅳ组(6/29 vs 23/29)。ADAM-19高表达组的平均生存时间为(28.44±2.41)个月,明显低于低表达组(43.58±4.27)个月,P<0.05。结论胶质瘤ADAM-19的表达与预后、WHO分级相关。展开更多
文摘去整合素基质金属蛋白酶19(a disintegrin and metalloproteinase 19,ADAM19)是新近发现的ADAMs(a disintegrin and metalloproteinase)家族新成员,在人胎盘组织中有较高水平的表达,但其在胎盘发生过程中母胎界面的时空表达及功能还鲜有报道.本研究首次就ADAM19在正常胎盘中的时空表达进行了研究,并以人绒毛膜癌细胞系JEG-3细胞为体外研究模型分析了ADAM19对滋养层细胞侵润和黏附的影响及其机理.研究结果显示,从空间上看,ADAM19在多种滋养层细胞中有广泛的分布,包括细胞滋养层细胞(vctb)、合体滋养层细胞(stb)和滋养层细胞柱(ct),绒毛内毛细血管的内皮细胞等.RT-PCR和Western印迹分析显示在孕早期8,9周龄绒毛中ADAM19的表达量较高,但在26周和足月胎盘中其mRNA和蛋白水平均明显下调.在JEG-3细胞中瞬时转染ADAM19可以降低其侵润能力同时增强细胞间的黏附.研究结果提示,ADAM19可能是胎盘发生过程中非常重要的滋养层细胞功能的调节分子.
基金Supported by the National Natural Science Foundation of China (Grant No. 30530760)the Knowledge Innovation Program of Chinese Academy of Sciences (Grant No. KSCX2-YW-R-53)a Program Enhancement Grant and Developing Scholar Award from Florida State University to Dr. QXA Sang
文摘Human ADAM19 is a recently identified member of the ADAM family.It is highly expressed in human placentas,but its dynamic change and function at the human feto-maternal interface during placentation remain to be elucidated.In this present study,the spatial and temporal expression and cellular localization of ADAM19 in normal human placentas were first demonstrated,and the effects of ADAM19 on trophoblast cell adhesion and invasion were further investigated by using a human choriocarcinoma cell line(JEG-3) as an in vitro model.The data demonstrated that ADAM19 was widely distributed in villous cytotrophoblast cells,syncytiotrophoblast cells,column trophoblasts,and villous capillary endothelial cells during early pregnancy.The mRNA and protein level of ADAM19 in placentas was high at gestational weeks 8-9,but diminished significantly at mid-and term pregnancy.In JEG-3 cells,the overexpression of ADAM19 led to diminished cell invasion,as well as increases in cell adhesiveness and the expression of E-cadherin,with no changes in β-catenin expression observed.These data indicate that ADAM19 may participate in the coordinated regulation of human trophoblast cell behaviors during the process of placentation.
文摘目的探讨解聚素-金属蛋白酶19水平与神经胶质瘤诊断、预后的相关性。方法收集初诊为原发神经胶质瘤的患者49例,回顾性分析胶质瘤ADAM-19的表达与临床因素的相关性,并结合远期随访结果对胶质瘤ADAM-19的表达水平与生存时间进行比较。结果 ADAM19的相对表达率在各组间的表达从小到大排列为对照组(0.57±0.13)<胶质瘤Ⅰ~Ⅱ级(1.24±0.46)<胶质瘤Ⅲ~Ⅳ级(3.78±0.85)。胶质瘤分型Ⅰ~Ⅱ组,ADAM-19低表达人数及所占比例明显高于高表达人数及比例(17/20 vs 3/20);相反的趋势可见于WHO分型Ⅲ~Ⅳ组(6/29 vs 23/29)。ADAM-19高表达组的平均生存时间为(28.44±2.41)个月,明显低于低表达组(43.58±4.27)个月,P<0.05。结论胶质瘤ADAM-19的表达与预后、WHO分级相关。
