In this research, Dioscorea membranacea Pierre was studied by using 30 samples from Khao Ruak Sub-district, Chai Badan District, Lop Buri Province, in Thailand. In this research, some morphology was studied including ...In this research, Dioscorea membranacea Pierre was studied by using 30 samples from Khao Ruak Sub-district, Chai Badan District, Lop Buri Province, in Thailand. In this research, some morphology was studied including shapes, leaves, stem colors, epidermal cells, stomata sizes and stipules. To study the genetic relationships, the AFLP technique and computer program were used. The Dioscorea membranacea Pierre was classified into 2 groups according to its phylogenetic type： the first group was ＂Hua Khao Yen Tai-Nuea＂ （Smilax corbularia Kunth）, and the second group included 30 further samples of Hua Khan Yen Tai （Dioscorea membranacea Pierre）. The ethanolic crude extract was also applied to test the anti-proliferative activity in the liver hepatocellular carcinoma （HepG2） cell lines which illustrates the characteristics of apoptosis： cell shrinkage, membrane blebbing and nuclear condensation. The expression ofBax gene is increased more than that of the control group while Bcl-2 gene which is anti-apoptotic is decreased. Furthermore, the result of western blot analysis reveals the up-regulation of Bax protein and down-regulation of Bcl-2 protein when compared with untreated cells. This might indicate that ethanolic crude extracts of Hua Khao Yen Tai could induce apoptosis and anti-proliferative on HepG2 cell lines, The results also revealed that some morphology cannot be used to predict which Dioscorea membranacea Pierre plants would be most effective.展开更多
文摘In this research, Dioscorea membranacea Pierre was studied by using 30 samples from Khao Ruak Sub-district, Chai Badan District, Lop Buri Province, in Thailand. In this research, some morphology was studied including shapes, leaves, stem colors, epidermal cells, stomata sizes and stipules. To study the genetic relationships, the AFLP technique and computer program were used. The Dioscorea membranacea Pierre was classified into 2 groups according to its phylogenetic type： the first group was ＂Hua Khao Yen Tai-Nuea＂ （Smilax corbularia Kunth）, and the second group included 30 further samples of Hua Khan Yen Tai （Dioscorea membranacea Pierre）. The ethanolic crude extract was also applied to test the anti-proliferative activity in the liver hepatocellular carcinoma （HepG2） cell lines which illustrates the characteristics of apoptosis： cell shrinkage, membrane blebbing and nuclear condensation. The expression ofBax gene is increased more than that of the control group while Bcl-2 gene which is anti-apoptotic is decreased. Furthermore, the result of western blot analysis reveals the up-regulation of Bax protein and down-regulation of Bcl-2 protein when compared with untreated cells. This might indicate that ethanolic crude extracts of Hua Khao Yen Tai could induce apoptosis and anti-proliferative on HepG2 cell lines, The results also revealed that some morphology cannot be used to predict which Dioscorea membranacea Pierre plants would be most effective.
