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The rotational anisotropies in the ferromagnetism/antiferromagnetism 1/ antiferromagnetism 2 exchange bias structures 被引量:2
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作者 胡经国 Stamps R L 《Chinese Physics B》 SCIE EI CAS CSCD 2006年第7期1595-1601,共7页
The rotational anisotropies in the exchange bias structures of ferromagnetism/antiferromagnetism 1/antiferro- magnetism 2 are studied in this paper. Based on the model, in which the antiferromagnetism is treated with ... The rotational anisotropies in the exchange bias structures of ferromagnetism/antiferromagnetism 1/antiferro- magnetism 2 are studied in this paper. Based on the model, in which the antiferromagnetism is treated with an Ising mean field theory and the rotational anisotropy is assumed to be related to the field created by the moment induced on the antiferromagnetic layer next to the ferromagnetic layer, we can explain why in experiments for ferromag- netism (FM)/antiferromagntism 1 (AFM1)/antiferromagnetism 2 (AFM2) systems the thickness-dependent rotational anisotropy value is non-monotonic, i.e. it reaches a minimum for this system at a specific thickness of the first anti- ferromagnetic layer and exhibits oscillatory behaviour. In addition, we find that the temperature-dependent rotational anisotropy value is in good agreement with the experimental result. 展开更多
关键词 FM/AFM1/afm2 trilayer rotational anisotropy exchange coupling
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Tuning Exchange Anisotropy of Exchange-Biased System
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作者 XU Yan HU Jing-Guo R.L. Stamps 《Communications in Theoretical Physics》 SCIE CAS CSCD 2008年第7期253-256,共4页
Exchange anisotropy in FM/AFM bilayers has given a lot of static magnetization properties such as enhanced coercivity and magnetization loop shifts. These phenomena are primarily from the effective anisotropies introd... Exchange anisotropy in FM/AFM bilayers has given a lot of static magnetization properties such as enhanced coercivity and magnetization loop shifts. These phenomena are primarily from the effective anisotropies introduced into a ferromagnet by exchange coupling with a strongly anisotropic antiferromagnet. These effective anisotropies can also be used to explain the dynamic consequences of exchange-biased bilayers. In this article, the dynamic consequences such as exchange-induced susceptibility, exchange-induced permeability, and the corresponding domain wall characteristics in the exchange-biased structures of ferromagnet/antiferromagnetl/antiferromagnet2 are studied. The results show that the second antiferromagnetic layer can largely affect the dynamic consequences of exchange-blazed bilayers. Especially in the case of critical temperature, the effects become more obvious. Practically, the exchange anisotropy of biased bilayer system can be tuned by exchange coupling with the second antiferromagnetic layer. 展开更多
关键词 Idomain effects SUSCEPTIBILITY PERMEABILITY FM/AFM1/afm2 trilayers
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免疫亲和层析净化-超高效液相色谱法测定生鲜牦牛乳中黄曲霉毒素M_1及M_2 被引量:8
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作者 刘云花 邓敬 +2 位作者 毛建霏 邹树良 马先果 《食品与发酵科技》 CAS 2019年第1期114-118,共5页
使用免疫亲和层析净化-超高效液相色谱法同时测定了生鲜牦牛乳中的黄曲霉毒素M_1(Aflatoxin M_1,AFM_1)及黄曲霉毒素M_2(Aflatoxin M_2,AFM_2)。黄曲霉毒素M_1仪器线性范围0.400-100μg/mL,仪器定量限0.400ng/mL,仪器检出限0.100ng/mL,... 使用免疫亲和层析净化-超高效液相色谱法同时测定了生鲜牦牛乳中的黄曲霉毒素M_1(Aflatoxin M_1,AFM_1)及黄曲霉毒素M_2(Aflatoxin M_2,AFM_2)。黄曲霉毒素M_1仪器线性范围0.400-100μg/mL,仪器定量限0.400ng/mL,仪器检出限0.100ng/mL,方法检出限0.005μg/kg,回收率85.2-95.5%;黄曲霉毒素M_2仪器线性范围0.100-100μg/mL,仪器定量限0.100ng/mL,仪器检出限0.03ng/mL,方法检出限0.0015μg/kg,回收率82.1-93.8%。使用该方法对来自若尔盖地区的10个生鲜牦牛乳进行了测定,结果表明生鲜牦牛乳中黄曲霉毒素M_1和M_2安全可控。 展开更多
关键词 超高效液相色谱 免疫亲和层析净化 黄曲霉毒素M1 黄曲霉毒素M2 牦牛乳
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三种FLAG融合标签的构建、表达及亲和常数测定 被引量:1
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作者 戴志昂 丁先锋 《浙江理工大学学报(自然科学版)》 2010年第2期313-317,共5页
FLAG融合标签是由8个氨基酸组成的短肽,可用于重组蛋白的免疫吸附纯化。为了建立一个基于FLAG融合标签的共表达纯化体系,将2个新的FLAG标签序列DYKDYKDYK和DYKDEDDK与FLAG标签的原有序列DYKDDDDK分别命名为FLAG1、FLAG2和FLAG3,利用PCR... FLAG融合标签是由8个氨基酸组成的短肽,可用于重组蛋白的免疫吸附纯化。为了建立一个基于FLAG融合标签的共表达纯化体系,将2个新的FLAG标签序列DYKDYKDYK和DYKDEDDK与FLAG标签的原有序列DYKDDDDK分别命名为FLAG1、FLAG2和FLAG3,利用PCR将FLAG标签融合到SHP2酪氨酸磷酸酶NSH2结构域序列的C端,并将其重组到原核表达载体pGEX-2T中,并在大肠杆菌中进行表达。利用GST亲和层析柱和分子筛层析柱纯化融合蛋白,Western blotting法检测其免疫活性。免疫印迹结果表明,3个FLAG融合标签都可以被抗-FLAG单克隆抗体M2(AFM2)特异性识别。利用ELISA技术测定3个FLAG标签与AFM2的亲和常数(1/Kd),其Kd值分别为0.143 80、.128 1、0.080 1μmol/L。 展开更多
关键词 FLAG融合标签 免疫吸附纯化 抗-FLAG单克隆抗体M2 亲和常数
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