目的检测研究所使用的人肾上腺皮质癌细胞SW-13、人胚肾细胞293T及人胃癌细胞AGS 3种细胞系污染情况,并探索短串联重复序列(short tandem repeat,STR)分型方法检测人类细胞系污染的可靠性。方法人类基因组上的微卫星位点携带着大量STR,...目的检测研究所使用的人肾上腺皮质癌细胞SW-13、人胚肾细胞293T及人胃癌细胞AGS 3种细胞系污染情况,并探索短串联重复序列(short tandem repeat,STR)分型方法检测人类细胞系污染的可靠性。方法人类基因组上的微卫星位点携带着大量STR,每个STR核心序列的重复次数随个体不同存在差异,作为一种DNA标记使得细胞系在DNA水平上具有个性化。通过荧光聚合酶链式反应体系扩增STR位点,检测的数据与DSMZ细胞库进行匹配以对细胞系进行鉴别。结果比对得出SW-13基本匹配,其中20个STR基因位点中只有TH01位点的两个等位基因重复数为7和8,与DSMZ细胞库中此等位基因重复数7和7有差异,其余均一致在可接受范围内。细胞系293T、AGS等位基因的重复数与DSMZ细胞库完全匹配。结论 STR分型检测方法被ATCC、DSMZ等权威机构推崇,且此研究采用20位点检测法涵盖ATCC-9位点及中检院-16位点检测法,该法权威可靠。本所3种细胞系遗传稳定,均无种内或种间污染。展开更多
It is well accepted in China that elder ginsengs have more bioactivity and value than younger ones. However, there is little research about the comparison of beneficial effects of ginsengs with different ages. In this...It is well accepted in China that elder ginsengs have more bioactivity and value than younger ones. However, there is little research about the comparison of beneficial effects of ginsengs with different ages. In this study, ginseng root extracts (GRE) were extracted from ginsengs of 5, 8, 12, 14, and 16 years old, respectively, using 55% ethanol and their effects on human leukemic K562 cells within 48 hours were tested by using Cell Counting Kit-8. The results show that there are significant increases in the cell viability of all the GRE groups compared with Control group within 32 hours. Furthermore, the growth curves of GRE groups were obviously distinct from each other. The cell viability of 5-year-old and 8-year-old GRE groups kept a rapid increase while that of 16-year-old GRE group showed a strong fluctuation within 28 hours. Our results demonstrate that root extracts from ginsengs of different ages contain different bioactivity constituents and have different effects on cell.展开更多
文摘目的检测研究所使用的人肾上腺皮质癌细胞SW-13、人胚肾细胞293T及人胃癌细胞AGS 3种细胞系污染情况,并探索短串联重复序列(short tandem repeat,STR)分型方法检测人类细胞系污染的可靠性。方法人类基因组上的微卫星位点携带着大量STR,每个STR核心序列的重复次数随个体不同存在差异,作为一种DNA标记使得细胞系在DNA水平上具有个性化。通过荧光聚合酶链式反应体系扩增STR位点,检测的数据与DSMZ细胞库进行匹配以对细胞系进行鉴别。结果比对得出SW-13基本匹配,其中20个STR基因位点中只有TH01位点的两个等位基因重复数为7和8,与DSMZ细胞库中此等位基因重复数7和7有差异,其余均一致在可接受范围内。细胞系293T、AGS等位基因的重复数与DSMZ细胞库完全匹配。结论 STR分型检测方法被ATCC、DSMZ等权威机构推崇,且此研究采用20位点检测法涵盖ATCC-9位点及中检院-16位点检测法,该法权威可靠。本所3种细胞系遗传稳定,均无种内或种间污染。
文摘It is well accepted in China that elder ginsengs have more bioactivity and value than younger ones. However, there is little research about the comparison of beneficial effects of ginsengs with different ages. In this study, ginseng root extracts (GRE) were extracted from ginsengs of 5, 8, 12, 14, and 16 years old, respectively, using 55% ethanol and their effects on human leukemic K562 cells within 48 hours were tested by using Cell Counting Kit-8. The results show that there are significant increases in the cell viability of all the GRE groups compared with Control group within 32 hours. Furthermore, the growth curves of GRE groups were obviously distinct from each other. The cell viability of 5-year-old and 8-year-old GRE groups kept a rapid increase while that of 16-year-old GRE group showed a strong fluctuation within 28 hours. Our results demonstrate that root extracts from ginsengs of different ages contain different bioactivity constituents and have different effects on cell.
