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重组HIV-1跨膜蛋白gp41在大肠杆菌中的表达
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作者 王静 王斌 +2 位作者 路永波 王秋波 张艳丽 《青岛大学医学院学报》 CAS 2000年第3期161-163,共3页
1目的 构建重组 HIV- 1SF2株跨膜蛋白 gp41基因片段的原核表达克隆并在大肠杆菌中表达。2方法 通过聚合酶链反应 (PCR)扩增目的基因 ,然后用限制性内切酶将其与原核表达载体 p MAL - p2定向连接 ,构建成重组质粒转入大肠杆菌 DH5α菌... 1目的 构建重组 HIV- 1SF2株跨膜蛋白 gp41基因片段的原核表达克隆并在大肠杆菌中表达。2方法 通过聚合酶链反应 (PCR)扩增目的基因 ,然后用限制性内切酶将其与原核表达载体 p MAL - p2定向连接 ,构建成重组质粒转入大肠杆菌 DH5α菌中。经 IPTG诱导 ,SDS- PAGE电泳分析有无重组蛋白表达。3结果 获得了重组的原核表达质粒及其表达产物。4结论 在 p MAL - p2中构建的 HIV - 1gp41重组质粒可在大肠杆菌中表达。 展开更多
关键词 重组HIV-1跨膜蛋白 大肠杆菌 质粒 基因重排 GP41 基因表达 聚合酶链反应 艾滋病 aid3
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一种健壮有效的决策树改进模型 被引量:3
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作者 卜亚杰 胡朝举 +1 位作者 白兰 董娜 《计算机应用》 CSCD 北大核心 2008年第B06期172-174,共3页
提出了一种健壮有效的决策树改进模型AID3。该决策树模型基于经典的ID3决策树模型,在属性的选取上进行了改进。利用引入属性优先关联度参数计算节点的修正信息增益,并选择具有最高修正增益的属性作为当前节点的测试属性。实验表明,AID3... 提出了一种健壮有效的决策树改进模型AID3。该决策树模型基于经典的ID3决策树模型,在属性的选取上进行了改进。利用引入属性优先关联度参数计算节点的修正信息增益,并选择具有最高修正增益的属性作为当前节点的测试属性。实验表明,AID3决策树模型在提高分类准确率的同时,有效地增强了模型的健壮性。 展开更多
关键词 决策树 ID3 属性优先关联度 aid3
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Application of computer aided 3D simulation technique for complicated foot and ankle fractures
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作者 章莹 《外科研究与新技术》 2011年第2期109-110,共2页
Objective To investigate the effect of computer aided 3D simulation technique for treating complicated foot and ankle fractures precisely.Methods From November 2007 to August 2009,255 patients with complicated foot an... Objective To investigate the effect of computer aided 3D simulation technique for treating complicated foot and ankle fractures precisely.Methods From November 2007 to August 2009,255 patients with complicated foot and ankle fractures 展开更多
关键词 Application of computer aided 3D simulation technique for complicated foot and ankle fractures
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Meditation on 2D and 3D Approach in Computer Aided Shape Design
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《Computer Aided Drafting,Design and Manufacturing》 2000年第2期45-49,共5页
关键词 Meditation on 2D and 3D Approach in Computer Aided Shape Design AEC CAD
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Computer-aided 3-D reconstruction and measurement of the optic canal and intracanalicular structures 被引量:1
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作者 陶海 马志中 +1 位作者 戴朴 姜荔 《Chinese Medical Journal》 SCIE CAS CSCD 2000年第2期44-47,共4页
Objective To reconstruct the human optic canal and its inner structures, and to provide detailed knowledge of this region for optic nerve decompression for further understanding on the pathologic mechanisms of indire... Objective To reconstruct the human optic canal and its inner structures, and to provide detailed knowledge of this region for optic nerve decompression for further understanding on the pathologic mechanisms of indirect optic nerve injury Methods Six optic canals and their inner structures were reconstructed using a computer aided 3 dimensional reconstruction system Quantitative measurement of the canal wall thickness, bony canal transverse area, optic nerve transverse area, dural sheath transverse area, subarachnoid space transverse area, and subarachnoid space volume were done by means of the computer morphometric analysis system The detailed spatial relationship among intracanalicular structures were also carefully identified on the 3 D models Results The thinnest portion of the canal was the middle part of the medial wall (0 45±0 35?mm) and the narrowest space was in the middle part of the optic canal (the transverse area was 18 21±2 50?mm 2) The volume of subarachnoid space which can be considered the compensatory space for distention incurred by the hemorrhage, optic nerve edema, or hematoma was 21 16±4 31?mm 3 At the cranial opening ,the middle part and orbital opening, its transverse area was 4 45±1 12?mm 2, 2 68±1 32?mm 2 and 1 23±0 83?mm 2, respectively Conclusions Since the compensatory space was limited, even a tiny amount of blood or swelling of the nerve may cause optic nerve compression Because the narrowest space was in the middle part of the optic canal and the compensatory space for distention gradually decreases from cranial end to orbital end, the middle part and the anterior part of the optic canal and dural sheath are critical in optic nerve decompression 展开更多
关键词 optic canal · optic nerve · computer aided · 3 D reconstruction
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CAD and CAE Integration Through Scientific Visualization Techniques for Illumination Design 被引量:10
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作者 Tiago Martinuzzi Buriol Sergio Scheer 《Tsinghua Science and Technology》 SCIE EI CAS 2008年第S1期26-33,共8页
Engineers tend to use different software to perform tasks such as geometry modeling, database management, numerical analysis, and visualization. This may cause decrease of productivity and loss of information during t... Engineers tend to use different software to perform tasks such as geometry modeling, database management, numerical analysis, and visualization. This may cause decrease of productivity and loss of information during the conversion process between different data file formats. This paper presents a computer aided design (CAD) and computer aided engineering (CAE) system integration using scientific visualization tools and techniques. It deals with the development of a 3D CAD add-in for lighting analysis which uses the CAD model as 3D interface for creating a lighting scheme, processing, and visualizing 2D or 3D illuminance fields. Visualization features as color and contour mapping were developed using the visualization toolkit (VTK) toolkit. The application integrates all functionalities of the 3D CAD with tools for light sources database management, pre-processing, processing, and post-processing of illuminance fields in a single environment. This approach increases productivity and eliminates the need for different software. 展开更多
关键词 illumination design scalar fields post-processing 3D computer aided design (CAD) visualization toolkit (VTK) systems integration
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STEP-based Feature Recognition System for B-spline Surface Features 被引量:2
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作者 Bitla Venu Venkateswara Rao Komma Deepanshu Srivastava 《International Journal of Automation and computing》 EI CSCD 2018年第4期500-512,共13页
The geometrical and topological information of 3D computer aided design (CAD) models should be represented as a neut- ral format file to exchange the data between different CAD systems. Exchange of 3D CAD model data... The geometrical and topological information of 3D computer aided design (CAD) models should be represented as a neut- ral format file to exchange the data between different CAD systems. Exchange of 3D CAD model data implies that the companies must exchange complete information about their products, all the way from design, manufacturing to inspection and shipping. This informa- tion should be available to each relevant partner over the entire life cycle of the product. This led to the development of an international standard organization (ISO) neutral format file named as standard for the exchange of product model data (STEP). It has been ob- served from the literature, the feature recognition systems developed were identified as planar, cylindrical, conical and to some extent spherical and toroidal surfaces. The advanced surface features such as B-spline and its subtypes are not identified. Therefore, in this work, a STEP-based feature recognition system is developed to recognize t--spline surface features and its sub-types from the 3D CAD model represented in AP203 neutral file format. The developed feature recognition system is implemented in Java programming language and the product model data represented in STEP AP203 format is interpreted through Java standard data access interface (JSDAI). The developed system could recognize B-spline surface features such as B-Spline surface with knots, quasi uniform surface, uniform surface, rational surface and Bezier surface. The application of extracted B-spline surface features information is discussed with reference to the toolpath generation for STEP-NC (STEP AP238). 展开更多
关键词 Feature recognition 3D computer aided design(CAD)model geometrical information standard for the exchange ofproduct model data(STEP)AP203 Java standard data access interface(JSDAI).
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