Alpase of Follicles during the Period before and after Ovulation in Superovulated Rabbits

The Alpas of follicles was studied systematically during the period before and after ovulation in superovulated rabbits. The locations and changes of follicular Alpase varied with the four kinds of physiological status before and after ovulation. Alpasc activity was the highest at oestrus and started to drop at the beginning of ovulation, then it increased 15 h later and decreased slightly 39 h afterwards the beginning of ovulation. Alpase was found on the follicular basement membrane at oestrus. The synthesis of fiber in fibcrblasts was connected with Alpasc activity.

梯度糖溶液对体外培养成骨细胞影响的实验研究

目的:探讨梯度高糖对体外培养成骨细胞(osteoblast,OB)的影响。方法:采用胰蛋白酶Ⅱ型胶原酶消化法从1～2日龄SD大鼠颅盖骨中分离出OB,倒置显微镜下及HE染色观察其形态,碱性磷酸酶(alkalinephosphatase,ALPase)染色法、钙化结节染色、VanGieson染色鉴定细胞后用不同浓度的糖溶液加入到成骨细胞培养体系中,作用不同时间,应用MTT比色法、ALPase含量测定、矿化结节形成量等来检测其对成骨细胞增殖和成骨能力的影响。结果:不同浓度的糖溶液在OB培养48h时具有明显的促进增殖作用,但>15mmol/L的溶液在72h时则呈抑制效应,其中以≤15mmol/L的糖溶液在培养早期有显著促进作用(P<0.05或P<0.01),而在培养较长时也无明显抑制效应;≥30mmol/L的糖溶液在OB培养7d时显著抑制ALPase的分泌(P<0.05);≥10mmol/L的糖溶液在培养18d时对矿化结节的形成也具有明显的延迟效应;<10mmol/L的糖溶液对OB分泌ALPase及矿化结节形成无抑制作用。结论:高糖可影响OB增殖、分化与矿化能力,OB增殖耐受的糖浓度为≤15mmol/L,OB分化与矿化过程耐受的糖浓度为<10mmol/L。

生骨再造散对家兔骨折愈合影响的实验研究

目的:检验生骨再造散促进骨折愈合的疗效,并探讨其治疗机制。方法:选用42只健康家兔,做双侧桡骨3mm缺损的骨折,随机分为实验组、药物对照组(仙灵骨葆)和空白对照组,分别以生骨再造散、仙灵骨葆和生理盐水灌胃给药,术后第1、2、3、4周采血,检测血清碱性磷酸酶(ALPase)。术后第14天和第31天分批处死兔子取桡骨标本,摄X线片,作生物力学测试和骨痂组织形态学观察。结果:骨折后第14天和第31天,实验组的X线片计分显示骨折修复优于空白对照组,生物力学性能和骨痂组织形态学均显著优于空白对照组(P<0.05,P<0.01),与药物对照组无显著性差异。ALPase在第1周和第4周各组间无显著性差异;第2周3组ALPase都不同程度升高,实验组和药物对照组均明显高于空白对照组,但实验组和药物对照组无差异;第3周实验组和药物对照组的ALPase均明显高于空白对照组,且实验组和药物对照组也有显著性差异(P<0.05,P<0.01)。结论:生骨再造散能缩短骨折愈合过程所需时间,提高骨折愈合质量,与仙灵骨葆疗效相似。

丹参对成骨细胞株MC3T3─E1细胞碱性磷酸酶活性及DNA合成影响的实验研究

选择克隆化成骨细胞株——ＭＣ３Ｔ３－Ｅ１细胞，采用细胞培养方法，观察细胞内ＤＮＡ合成与碱性磷酸酶（ＡＬＰａｓｅ）活性，了解丹参对体外培养的成骨细胞生长、分化与代谢的影响。研究结果显示，丹参明显增强ＭＣ３Ｔ３－Ｅ１细胞内ＡＬＰａｓｅ活性，５．０ｍｇ／ｍｌ浓度时，丹参对ＭＣ３Ｔ３－Ｅ１细胞内碱性磷酸酶活性影响最大，比对照组增强约１３５％。丹参这种促进作用只限于分化晚期的ＭＣ３Ｔ３－Ｅ１细胞，而对分化早期细胞内碱性磷酸酶活性产生抑制作用。其次，这种作用还与药物作用时间有关。但是，丹参对各生长分化期的ＭＣ３Ｔ３－Ｅ１细胞内ＤＮＡ合成均无影响。本研究结果表明，丹参有明显增强体外培养分化晚期的ＭＣ３Ｔ３－Ｅ１细胞内碱性磷酸酶活性的作用，这种作用不是通过促进细胞增殖，而是改善细胞功能实现的，并且受丹参药物浓度与作用时间的影响，说明丹参可能在促进正畸牙齿移动中新生牙槽骨形成方面发挥作用。

Effects of salvia miltiorrhiza bung （SMB） onosteoblast－like cell lin （clonal MC3T3－E1 cells）

Objective:To investigate the role of SMB on the growth , differentiation and metabolism of osteoblastlike cells in vitro, we studied the effects of SMB on DNA synthesis and alkaline phosphatase(ALPase) activity of the cloned osteoblast like cells-MC3T3-E1. Methods: The cells were cultured in α-MEM with 0. 3% of fetal bovine serum and treated with SMB at the concentration of 0. 1 - 10. 0 g/L. Results : There was no significant difference in DNA synthesis between the groups in different concentration of SMB and the group of control. But SMB increased ALPase activity in a concentration-dependent fashion in later stage of cells , and up to maximum at the level of 5. 0 g/L , in which concentration , ALPase activity was about 135 % greater than that of control. However ,ALPase activity was inhibited in early stage of cells by the addition of SMB. Conclusion : SMB has the stimulating effect on the activity of osteoblast-like cells in vitro, and may play an important role in accelerating the remodeling of bone in vivo as well.

FGF18对成牙本质细胞样细胞增殖和碱性磷酸酶活性的影响

目的:研究FGF18对成牙本质细胞样细胞MDPC-23增殖和ALPase活性的影响。方法:用MTT法和酶动力法分别检测MDPC-23细胞经不同浓度的FGF18刺激48 h和72 h后细胞增殖和ALPase活性的变化。结果:在一定范围内,FGF18显著促进MDPC-23细胞的增殖和ALPase活性,随着浓度的增高和时间的延长,作用更加明显。结论:FGF18可能促进小鼠成牙本质细胞的增殖和分化。