[ Objective] The paper aimed to understand the epidemiological characteristics of Brucella strains in Xinjiang and then provide an available integrated measure to prevent and control brucellosis. [ Method ] Eleven sus...[ Objective] The paper aimed to understand the epidemiological characteristics of Brucella strains in Xinjiang and then provide an available integrated measure to prevent and control brucellosis. [ Method ] Eleven suspected Brucella strains were isolated by traditional methods, which were further identified by AMOS-PCR assay. Conventional biochemical tests were carried out to identify the biological subtype of sheep Brucella. [ Result] Nine strains were all B. meliten- s/s, and biological test indicated that all of them were B. melitensis biotype 3. [ Conclusion] B. melitensis biotype 3 was the predominant strain of Brucella in Xin- jiang, and AMOS-PCR assay could be applied safely and quickly as an assistant tool to detect Brucella. The results of molecular epidemiology laid a foundation for updating prevention and control strategy against brucellosis in Xinjiang.展开更多
文摘目的掌握江苏省2018年人感染布鲁氏菌主要流行株的种型和基因型。方法运用普通PCR及AMOS多重PCR确认分离株的生物种型;采用多位点序列分型(Multilocus sequence analysis,MLSA)和多位点串联重复序列分析(Multiple-locus variable number tandem repeat analysis,MLVA)鉴定基因型,并与国内外流行株进行聚类分析。结果2018年共分离到56株布鲁氏菌,MLSA分型显示一株菌为猪种布鲁氏菌ST(Sequence type)17型,其他均为羊种布鲁氏菌ST8型。MLVA将56株菌分为47个基因亚型(46个羊种,1个猪种),聚类显示羊种布鲁氏菌全部为“东地中海簇”。结论2018年江苏省人感染布病主要为“东地中海簇”的ST8型羊种布鲁氏菌,并首次发现一例人感染ST17型猪种布鲁氏菌。
基金Supported by Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture"Animal Disease Prevention and Control Technology System in Border Areas"(201103008)National Key Technology R&D Program"Integration and Demonstration of Production-Life-Ecosystem Safeguard Technique in Xinjiang Desert Arid Oasis Steppe Region"(2012BAD13B03)
文摘[ Objective] The paper aimed to understand the epidemiological characteristics of Brucella strains in Xinjiang and then provide an available integrated measure to prevent and control brucellosis. [ Method ] Eleven suspected Brucella strains were isolated by traditional methods, which were further identified by AMOS-PCR assay. Conventional biochemical tests were carried out to identify the biological subtype of sheep Brucella. [ Result] Nine strains were all B. meliten- s/s, and biological test indicated that all of them were B. melitensis biotype 3. [ Conclusion] B. melitensis biotype 3 was the predominant strain of Brucella in Xin- jiang, and AMOS-PCR assay could be applied safely and quickly as an assistant tool to detect Brucella. The results of molecular epidemiology laid a foundation for updating prevention and control strategy against brucellosis in Xinjiang.
