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绵羊ANGPTL4蛋白在脂肪组织中的表达研究 被引量:1
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作者 张静 张方 +3 位作者 胡子乔 潘洋洋 乔利英 刘文忠 《山西农业大学学报(自然科学版)》 CAS 2015年第6期644-648,共5页
血管生成素样蛋白4(ANGPTL4)是一种与脂肪代谢相关的分泌性蛋白。本研究利用Western blotting技术检测了两个不同品种的脂尾型绵羊,即广灵大尾羊和小尾寒羊的2、4、6、8、10和12月龄时的母羊(每年龄段2只),共24个个体的皮下、腹膜后、... 血管生成素样蛋白4(ANGPTL4)是一种与脂肪代谢相关的分泌性蛋白。本研究利用Western blotting技术检测了两个不同品种的脂尾型绵羊,即广灵大尾羊和小尾寒羊的2、4、6、8、10和12月龄时的母羊(每年龄段2只),共24个个体的皮下、腹膜后、肾周和尾部脂肪组织的ANGPTL4蛋白的表达量。结果表明:品种、月龄和组织对ANGPTL4蛋白的表达都有极显著(P<0.001)影响。品种与月龄的互作对该蛋白表达也有显著(P<0.01)影响。本研究结果为进一步探讨ANGPTL4蛋白在绵羊脂肪组织中的作用机制提供了理论依据。 展开更多
关键词 绵羊 ANGPTL4蛋白 脂肪组织 WESTERN BLOTTING 表达
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Effect of human angiopoietin-like protein 4 overexpression on the growth of esophageal carcinoma EC9706 cells
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作者 Shangen Zheng Fang Wang +3 位作者 Yinjuan Ding Lifang Zhang Qianchuan Huang Guoqiang Zhao 《The Chinese-German Journal of Clinical Oncology》 CAS 2010年第2期101-105,共5页
Objective: The aim of the study was to construct the eukaryotic expression vector of human angiopoietin-like protein 4 (ANGPTL4) and observe the effect of ANGPTL4 overexpression on the growth of esophageal carcinom... Objective: The aim of the study was to construct the eukaryotic expression vector of human angiopoietin-like protein 4 (ANGPTL4) and observe the effect of ANGPTL4 overexpression on the growth of esophageal carcinoma EC9706 cells. Methods: Total RNA was extracted from normal hepatic tissue, and ANGPTL4 cDNA was amplified by RT-PCR. The PCR product was doubly digested by Xbal and Sail, and then recombined into eukaryotic expression vector. Then, plRES-GFP-ANGPTL4 was obtained by G418 selection, then plRES-GFP-ANGPTL4 and plRES-GFP were transfected into EC9706 cells with lipidosome-packaged method. Meanwhile, the transfected cells were selected by G418, and then stable transfected cell lines were obtained. ANGPTL4 mRNA levels, the celt cycles and growth curves of EC9706 cells in experiment group (transfected with plRES-GFP-ANGPTL4), empty vector group (transfected with plRES-GFP) and blank control group (EC9706 cells without transfection) were detected with RT-PCR, flow cytometry and MTT methods, respectively. Results: Eukaryotic ANGPTL4 expression vector plRES-GFP-ANGPTL4 was successfully constructed. The ANGPTL4 mRNA level (0.21 ± 0.03) in experiment group was significantly higher than that of the empty vector group (0.04 ± 0.008) and the blank control group (0.05 ± 0.007), with significant differences (P 〈 0.01). The proportion of cells in S phase in experiment group was significantly different with those of the other two groups (P 〈 0.05). The cell growth of EC9706 cells in experiment group was slower than those of the other two groups. From the third day, the differences began to be significant. Conclusion: ANGPTL4 overexpression in esophageal carcinoma EC9706 cells could inhibit the growth of EC9706 cells. 展开更多
关键词 angiopoietin-like protein 4 (ANGPTL4) esophageal carcinoma GROWTH transfection
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