The antisense transcript long non-coding RNA（lnc RNA）（antisense non-coding RNA in the INK4 locus, ANRIL） is an antisense of the cyclin-dependent kinase inhibitor 2 B（CDKN2B） gene on chromosome 9 p21 that contain...The antisense transcript long non-coding RNA（lnc RNA）（antisense non-coding RNA in the INK4 locus, ANRIL） is an antisense of the cyclin-dependent kinase inhibitor 2 B（CDKN2B） gene on chromosome 9 p21 that contains an overlapping 299-bp region and shares a bidirectional promoter with alternate open reading frame（ARF）. In the context of gene regulation, ANRIL is responsible for directly recruiting polycomb group（Pc G） proteins, including polycomb repressive complex-1（PRC-1） and polycomb repressive complex-2（PRC-2）, to modify the epigenetic chromatin state and subsequently inhibit gene expression in cis-regulation. On the other hand, previous reports have indicated that ANRIL is capable of binding to a specific site or sequence, including the Alu element, E2 F transcription factor 1（E2F1）, and CCCTC-binding factor（CTCF）, to achieve trans-regulation functions. In addition to its function in cell proliferation, adhesion and apoptosis, ANRIL is very closely associated with atherosclerosis-related diseases. The different transcripts and the SNPs that are related to atherosclerotic vascular diseases（ASVD-SNPs） are inextricably linked to the development and progression of atherosclerosis. Linear transcripts have been shown to be a risk factor for atherosclerosis, whereas circular transcripts are protective against atherosclerosis. Furthermore, ANRIL also acts as a component of the inflammatory pathway involved in the regulation of inflammation, which is considered to be one of the causes of atherosclerosis. Collectively, ANRIL plays an important role in the formation of atherosclerosis, and the artificial modification of ANRIL transcripts should be considered following the development of this disease.展开更多
目的探讨ANRIL(antisense noncoding RNA in the INK4locus,ANRIL)基因与中国川东北汉族人群动脉粥样硬化性脑梗死(Atherosclerotic stroke,ATS)的相关性。方法选择我国川东北地区汉族人群为研究对象,采用病例-对照研究,以200例ATS患者...目的探讨ANRIL(antisense noncoding RNA in the INK4locus,ANRIL)基因与中国川东北汉族人群动脉粥样硬化性脑梗死(Atherosclerotic stroke,ATS)的相关性。方法选择我国川东北地区汉族人群为研究对象,采用病例-对照研究,以200例ATS患者作为病例组,同期体检的健康人群205例作为对照组进行研究。对ATS发病常见的环境危险因素和ANRIL基因4个单核甘酸多态性位点(SNP)(rs1537378,rs7865618,rs10757274,rs2383206)进行分析,比较不同基因型与ATS发病风险的相关性,并根据性别和年龄进一步分组,进行亚组分析研究ATS是否具有年龄及性别遗传特异性。结果超重、血脂异常与ATS显著相关(P<0.05);rs1537378多态位点的GG基因型、rs7865618多态位点的AA基因型与男性ATS显著相关(P<0.05),rs1537378多态位点的GG基因型与年龄≥45岁人群ATS显著相关(P<0.05),均显著增加其ATS易感性。结论超重、血脂异常是ATS的危险因素;ANRIL基因SNPs与中国川东北汉族人群ATS相关。展开更多
With the rising interest in the regulatory functions of long non-coding RNAs （IncRNAs） in complex human diseases such as cardiovascular diseases, there is an increasing need in public databases offering comprehensiv...With the rising interest in the regulatory functions of long non-coding RNAs （IncRNAs） in complex human diseases such as cardiovascular diseases, there is an increasing need in public databases offering comprehensive and integrative data for all aspects of these versatile molecules. Recently, a variety of public data repositories that specialized in lncRNAs have been developed, which make use of huge high-throughput data particularly from next-generation sequencing （NGS） approaches. Here, we provide an overview of current IncRNA databases covering basic and functional annotation, IncRNA expression and regulation, interactions with other biomolecules, and genomic variants influencing the structure and function of lncRNAs. The prominent IncRNA antisense noncoding RNA in the INK4 locus （ANRIL）, which has been unequivocally associated with coronary artery disease through genome-wide association studies （GWAS）, serves as an example to demonstrate the features of each individual database.展开更多
基金supported by grants from the Shenzhen Science and Technology Project(No.201401027)
文摘The antisense transcript long non-coding RNA（lnc RNA）（antisense non-coding RNA in the INK4 locus, ANRIL） is an antisense of the cyclin-dependent kinase inhibitor 2 B（CDKN2B） gene on chromosome 9 p21 that contains an overlapping 299-bp region and shares a bidirectional promoter with alternate open reading frame（ARF）. In the context of gene regulation, ANRIL is responsible for directly recruiting polycomb group（Pc G） proteins, including polycomb repressive complex-1（PRC-1） and polycomb repressive complex-2（PRC-2）, to modify the epigenetic chromatin state and subsequently inhibit gene expression in cis-regulation. On the other hand, previous reports have indicated that ANRIL is capable of binding to a specific site or sequence, including the Alu element, E2 F transcription factor 1（E2F1）, and CCCTC-binding factor（CTCF）, to achieve trans-regulation functions. In addition to its function in cell proliferation, adhesion and apoptosis, ANRIL is very closely associated with atherosclerosis-related diseases. The different transcripts and the SNPs that are related to atherosclerotic vascular diseases（ASVD-SNPs） are inextricably linked to the development and progression of atherosclerosis. Linear transcripts have been shown to be a risk factor for atherosclerosis, whereas circular transcripts are protective against atherosclerosis. Furthermore, ANRIL also acts as a component of the inflammatory pathway involved in the regulation of inflammation, which is considered to be one of the causes of atherosclerosis. Collectively, ANRIL plays an important role in the formation of atherosclerosis, and the artificial modification of ANRIL transcripts should be considered following the development of this disease.
文摘目的探讨ANRIL(antisense noncoding RNA in the INK4locus,ANRIL)基因与中国川东北汉族人群动脉粥样硬化性脑梗死(Atherosclerotic stroke,ATS)的相关性。方法选择我国川东北地区汉族人群为研究对象,采用病例-对照研究,以200例ATS患者作为病例组,同期体检的健康人群205例作为对照组进行研究。对ATS发病常见的环境危险因素和ANRIL基因4个单核甘酸多态性位点(SNP)(rs1537378,rs7865618,rs10757274,rs2383206)进行分析,比较不同基因型与ATS发病风险的相关性,并根据性别和年龄进一步分组,进行亚组分析研究ATS是否具有年龄及性别遗传特异性。结果超重、血脂异常与ATS显著相关(P<0.05);rs1537378多态位点的GG基因型、rs7865618多态位点的AA基因型与男性ATS显著相关(P<0.05),rs1537378多态位点的GG基因型与年龄≥45岁人群ATS显著相关(P<0.05),均显著增加其ATS易感性。结论超重、血脂异常是ATS的危险因素;ANRIL基因SNPs与中国川东北汉族人群ATS相关。
文摘With the rising interest in the regulatory functions of long non-coding RNAs （IncRNAs） in complex human diseases such as cardiovascular diseases, there is an increasing need in public databases offering comprehensive and integrative data for all aspects of these versatile molecules. Recently, a variety of public data repositories that specialized in lncRNAs have been developed, which make use of huge high-throughput data particularly from next-generation sequencing （NGS） approaches. Here, we provide an overview of current IncRNA databases covering basic and functional annotation, IncRNA expression and regulation, interactions with other biomolecules, and genomic variants influencing the structure and function of lncRNAs. The prominent IncRNA antisense noncoding RNA in the INK4 locus （ANRIL）, which has been unequivocally associated with coronary artery disease through genome-wide association studies （GWAS）, serves as an example to demonstrate the features of each individual database.
