In order to understand the functions of ARF（Auxin Response Factor） gene family during the growth and development and under stress in pepper, 20 ARF genes were identified from the capsicum genome by bioinformatics an...In order to understand the functions of ARF（Auxin Response Factor） gene family during the growth and development and under stress in pepper, 20 ARF genes were identified from the capsicum genome by bioinformatics and these genes were not well-distributed on 12 chromosomes of pepper. The results of the phylogeny relationship showed that ARF genes of pepper could be divided into Class I and II and could be further divided into seven sub-groups： Ia, Ib, Ic, Id, Ie, IIa and IIb. The results of gene structure showed that ARF gene family members were composed of 1-15 exons, and for each gene expression level of different tissues at different developmental stages revealed that the gene family had certain expression specificity. The qRT-PCR uncovered that high salt stress could significantly activate and inhibit the expression ofARF gene family.展开更多
Objective： This study was designed to investigate promoter methylation status and protein expression of p14^ARF gene in non-small cell lung cancer, and value the role of p14^ARF promoter methylation in carcinogenesis...Objective： This study was designed to investigate promoter methylation status and protein expression of p14^ARF gene in non-small cell lung cancer, and value the role of p14^ARF promoter methylation in carcinogenesis of non-small cell lung cancer. Methods： Promoter methylation status and protein expression of p14^ARF gene in 40 cases of non-small cell lung cancer were analyzed by methylation specific polymerase china reaction （MSP）, restriction enzyme-related polymerase chain reaction （RE-PCR） and immunohistochemistry （IHC）. Results： The positive rates of p14^ARF promoter methylation in tumor tissues and normal tissues adjacent to cancer were 17.5% （7/40） and 2.5% （1/40） respectively. There were statistically significant differences between them, P〈0.05. The results of RE-PCR were consistent with that of MSP. The expression rate of p14^ARF protein in tumor tissues was significantly lower than that in normal tissues adjacent to cancer, p〈0.01. Promoter methylation status and protein expression of p14^ARF gene in non-small cell lung cancer showed significantly an inverse correlation （r=-0.56, P〈0.01）, and both of them did not relate statistically with the clinicopathologic characteristics of patients such as histological classification, clinical stage, differentiation grade and lymph node involvement. Conclusion： Promoter methylation is a crucial mechanism of inactivation of p14^ARF gene. Promoter methylation of p14^ARF gene might he involved in carcinogenesis of non-small cell lung cancer, and is an early event in development process of non-small cell lung cancer. It might be used as a new target in gene treatments in the future.展开更多
文摘In order to understand the functions of ARF（Auxin Response Factor） gene family during the growth and development and under stress in pepper, 20 ARF genes were identified from the capsicum genome by bioinformatics and these genes were not well-distributed on 12 chromosomes of pepper. The results of the phylogeny relationship showed that ARF genes of pepper could be divided into Class I and II and could be further divided into seven sub-groups： Ia, Ib, Ic, Id, Ie, IIa and IIb. The results of gene structure showed that ARF gene family members were composed of 1-15 exons, and for each gene expression level of different tissues at different developmental stages revealed that the gene family had certain expression specificity. The qRT-PCR uncovered that high salt stress could significantly activate and inhibit the expression ofARF gene family.
文摘Objective： This study was designed to investigate promoter methylation status and protein expression of p14^ARF gene in non-small cell lung cancer, and value the role of p14^ARF promoter methylation in carcinogenesis of non-small cell lung cancer. Methods： Promoter methylation status and protein expression of p14^ARF gene in 40 cases of non-small cell lung cancer were analyzed by methylation specific polymerase china reaction （MSP）, restriction enzyme-related polymerase chain reaction （RE-PCR） and immunohistochemistry （IHC）. Results： The positive rates of p14^ARF promoter methylation in tumor tissues and normal tissues adjacent to cancer were 17.5% （7/40） and 2.5% （1/40） respectively. There were statistically significant differences between them, P〈0.05. The results of RE-PCR were consistent with that of MSP. The expression rate of p14^ARF protein in tumor tissues was significantly lower than that in normal tissues adjacent to cancer, p〈0.01. Promoter methylation status and protein expression of p14^ARF gene in non-small cell lung cancer showed significantly an inverse correlation （r=-0.56, P〈0.01）, and both of them did not relate statistically with the clinicopathologic characteristics of patients such as histological classification, clinical stage, differentiation grade and lymph node involvement. Conclusion： Promoter methylation is a crucial mechanism of inactivation of p14^ARF gene. Promoter methylation of p14^ARF gene might he involved in carcinogenesis of non-small cell lung cancer, and is an early event in development process of non-small cell lung cancer. It might be used as a new target in gene treatments in the future.
