目的构建表型重叠家系相关基因SCN5A del QKP1507-1509突变型真核表达载体,研究其在H9C2细胞中的表达。方法一步法定点诱变技术构建SCN5A基因del QKP1507-1509突变型真核表达载体,电穿孔方法将野生型和突变型质粒转染至H9C2细胞,应用实...目的构建表型重叠家系相关基因SCN5A del QKP1507-1509突变型真核表达载体,研究其在H9C2细胞中的表达。方法一步法定点诱变技术构建SCN5A基因del QKP1507-1509突变型真核表达载体,电穿孔方法将野生型和突变型质粒转染至H9C2细胞,应用实时荧光定量聚合酶链反应(RFQ-PCR)及Western blot技术检测其mRNA及蛋白表达情况。结果琼脂糖凝胶电泳法及DNA直接测序法均表明定点突变成功,RFQ-PCR及Western blot结果显示,野生组、突变组和混合组SCN5A基因mRNA相对表达量分别为1.089±0.459、1.011±0.840和1.069±0.492,3组SCN5A基因mRNA相对表达量比较差异无统计学意义(P>0.05)。野生组、突变组和混合组SCN5A蛋白在H9C2细胞的相对表达量分别为3.781±0.221、3.466±0.176和3.714±0.198,3组SCN5A蛋白相对表达量比较差异无统计学意义(P>0.05)。结论成功构建钠通道基因del QKP1507-1509突变型真核表达载体并转染至H9C2细胞,该突变未引起钠通道在细胞膜的异常表达,为进一步功能研究提供了研究基础及方向。展开更多
The novel polyoxometalate, 32[Mo8ⅣMo4ⅤV2ⅣO38(PO4)], was synthesized and characterized by elementary analysis, EPR, IR spectra and X ray diffraction. The compoundcrystallizes in triclinic system, space group with a=...The novel polyoxometalate, 32[Mo8ⅣMo4ⅤV2ⅣO38(PO4)], was synthesized and characterized by elementary analysis, EPR, IR spectra and X ray diffraction. The compoundcrystallizes in triclinic system, space group with a= 1.41999(2)nm, b=1.43467(2)nm, c=1.694610(10)nm, α=95.7250(10)°, β=92.2110(10)°, γ=92.6060(10)°, V=3.42829(7)nm3, Z=2, Dc=2.388g·cm-3, Mr=2465.10g·mol-1, μ=2.489mm-1, F(000)=2388, R1=0.0584, wR2=0.1461, S=1.164. The heteropolyanion is a bi capped pseudo Keggin complex. CCDC: 186645.展开更多
文摘The novel polyoxometalate, 32[Mo8ⅣMo4ⅤV2ⅣO38(PO4)], was synthesized and characterized by elementary analysis, EPR, IR spectra and X ray diffraction. The compoundcrystallizes in triclinic system, space group with a= 1.41999(2)nm, b=1.43467(2)nm, c=1.694610(10)nm, α=95.7250(10)°, β=92.2110(10)°, γ=92.6060(10)°, V=3.42829(7)nm3, Z=2, Dc=2.388g·cm-3, Mr=2465.10g·mol-1, μ=2.489mm-1, F(000)=2388, R1=0.0584, wR2=0.1461, S=1.164. The heteropolyanion is a bi capped pseudo Keggin complex. CCDC: 186645.