We investigated the relationship between muscle inosine monophosphate (IMP) content and mRNA levels of ADSL, AMPD1, and ATIC in Dapulian (DPL), Landrace × Dapulian (LDPL), and Duroc × Landrace × Dapulia...We investigated the relationship between muscle inosine monophosphate (IMP) content and mRNA levels of ADSL, AMPD1, and ATIC in Dapulian (DPL), Landrace × Dapulian (LDPL), and Duroc × Landrace × Dapulian (DLDPL) hybridized pigs. Methods: The total RNA in longissimus dorsi was isolated from Dapulian (DPL), Landrace × Dapulian (LDPL) and Duroc × Landrace × Dapulian (DLDPL) hybridized pigs, weighed about 95.0 kg, n = 8/species. The internal genes with highest stability (YWHAZ and RPL4) were chosen from 11 common internal genes using Quantitative real-time PCR (qPCR) and geNorm software. The mRNA levels of ADSL, AMPD1 and ATIC genes were corrected with YWHAZ and RPL4 genes. The muscular IMP content was determined by HPLC. The muscular IMP content in DPL was higher than that in LDPL and DLDPL, 25.00% (p 0.05) and 15.56% (p > 0.05), respectively. The muscular mRNA level of ADSL gene in DPL and LDPL was higher than that in DLDPL, 24.14% and 12.07%, respectively (p 0.05). The muscular mRNA level of ATIC gene in DPL and LDPL was higher than that in DLDPL, 66.67% and 33.33%, respectively (p 0.05). The muscular mRNA level of AMPD1 gene in DPL and LDPL was higher than that in DLDPL, 14.49% and 33.26%, respectively. Furthermore, the IMP content was positively correlated with the mRNA level of ADSL, AMPD1 and ATIC genes, respectively (p 0.05). The mRNA level of ADSL gene was highly related to that of AMPD1 and ATIC gene, respectively (p 0.01), while that of AMPD1 gene was not strongly correlated with that of ATIC gene (p > 0.05). The muscular mRNA level of AMPD1, ADSL and ATIC genes and the muscular IMP content in DPL were highest, followed by those in LDPL and DLDPL. The muscular IMP content was positively correlated with the muscular mRNA level of ADSL, AMPD1 and ATIC genes, respectively.展开更多
Puerarin is a natural isoflavone isolated from plants of the genus Pueraria and functions as a protector against cerebral ischemia. We hypothesized that puerarin can be involved in the repair of peripheral nerve injur...Puerarin is a natural isoflavone isolated from plants of the genus Pueraria and functions as a protector against cerebral ischemia. We hypothesized that puerarin can be involved in the repair of peripheral nerve injuries. To test this hypothesis, doses of 10, 5, or 2.5 mg/kg per day puer- arin （8-（β-D-Glucopyranosyl-7-hydroxy-3-（4-hydroxyphenyl）-4H-l-benzopyran-4-one） were injected intraperitoneally into mouse models of sciatic nerve injury. Puerarin at the middle and high doses significantly up-regulated the expression of growth-associated protein 43 in the L4_6 segments of the spinal cord from mice at 1, 2, and 4 weeks after modeling, and reduced the atro- phy of the triceps surae on the affected side and promoted the regeneration of nerve fibers of the damaged spinal cord at 8 weeks after injury. We conclude that puerarin exerts an ongoing role to activate growth-associated protein 43 in the corresponding segment of the spinal cord after sciat- ic nerve injury, thus contributing to neural regeneration after sciatic nerve injuries.展开更多
Objective To investigate the significance of d ecompression of optic nerve in the re habilitation of traumatic optic neu ropathy following head trauma.Method Surgical treatment was performed on16patients suffered from...Objective To investigate the significance of d ecompression of optic nerve in the re habilitation of traumatic optic neu ropathy following head trauma.Method Surgical treatment was performed on16patients suffered from traumatic neuropathy following head trau-ma.Fragments ,hematoma,necrotic b rain tissue resulting from fracture of orbital bone were removed and fron tal and orbital fracture or malpo-sition corrected by craniotomy thro ugh valve of frontal bone in 15patien ts.Optic canal was probed and sheath of optic nerve was cut open for decompression of optic nerve.Front otemporal wound was made in 1case with superior orbital fissure syndrome and decompression of optic canal and abrasion of superior orbital fis sure were performed through outer side of orbital point.Result Surgical treatment was effective in12cases(75%),exophthalmus in 2cases was corrected.Conclusion Decompression of optic nerve throug h frontal bone or frontotemporal is e ffective in enhancing visual acuity in patients with traumatic optic neuropathy following head trauma.展开更多
The planning of prior conservation areas is an important part of nature re- serve system, ：it is of great significance for biodiversity conservation. The methods of priority planning include hot-spots analysis, gap a...The planning of prior conservation areas is an important part of nature re- serve system, ：it is of great significance for biodiversity conservation. The methods of priority planning include hot-spots analysis, gap analysis, systematic conservation planning analysis and multi-criteria decision analysis. The key issues of priority area planning include the determination of the protect objects, the analyses of the protec- tion value and protection cost, the win-win development of protection and economy, and the changes of the management strategies of the protected areas. In this study, the features and research progress of different conservation methods were analyzed, and based on the discussion on the key issues of the planning of the protected areas, the application prospect of different methods was analyzed.展开更多
文摘We investigated the relationship between muscle inosine monophosphate (IMP) content and mRNA levels of ADSL, AMPD1, and ATIC in Dapulian (DPL), Landrace × Dapulian (LDPL), and Duroc × Landrace × Dapulian (DLDPL) hybridized pigs. Methods: The total RNA in longissimus dorsi was isolated from Dapulian (DPL), Landrace × Dapulian (LDPL) and Duroc × Landrace × Dapulian (DLDPL) hybridized pigs, weighed about 95.0 kg, n = 8/species. The internal genes with highest stability (YWHAZ and RPL4) were chosen from 11 common internal genes using Quantitative real-time PCR (qPCR) and geNorm software. The mRNA levels of ADSL, AMPD1 and ATIC genes were corrected with YWHAZ and RPL4 genes. The muscular IMP content was determined by HPLC. The muscular IMP content in DPL was higher than that in LDPL and DLDPL, 25.00% (p 0.05) and 15.56% (p > 0.05), respectively. The muscular mRNA level of ADSL gene in DPL and LDPL was higher than that in DLDPL, 24.14% and 12.07%, respectively (p 0.05). The muscular mRNA level of ATIC gene in DPL and LDPL was higher than that in DLDPL, 66.67% and 33.33%, respectively (p 0.05). The muscular mRNA level of AMPD1 gene in DPL and LDPL was higher than that in DLDPL, 14.49% and 33.26%, respectively. Furthermore, the IMP content was positively correlated with the mRNA level of ADSL, AMPD1 and ATIC genes, respectively (p 0.05). The mRNA level of ADSL gene was highly related to that of AMPD1 and ATIC gene, respectively (p 0.01), while that of AMPD1 gene was not strongly correlated with that of ATIC gene (p > 0.05). The muscular mRNA level of AMPD1, ADSL and ATIC genes and the muscular IMP content in DPL were highest, followed by those in LDPL and DLDPL. The muscular IMP content was positively correlated with the muscular mRNA level of ADSL, AMPD1 and ATIC genes, respectively.
基金supported by the National Natural Science Foundation of China,No.81250016
文摘Puerarin is a natural isoflavone isolated from plants of the genus Pueraria and functions as a protector against cerebral ischemia. We hypothesized that puerarin can be involved in the repair of peripheral nerve injuries. To test this hypothesis, doses of 10, 5, or 2.5 mg/kg per day puer- arin （8-（β-D-Glucopyranosyl-7-hydroxy-3-（4-hydroxyphenyl）-4H-l-benzopyran-4-one） were injected intraperitoneally into mouse models of sciatic nerve injury. Puerarin at the middle and high doses significantly up-regulated the expression of growth-associated protein 43 in the L4_6 segments of the spinal cord from mice at 1, 2, and 4 weeks after modeling, and reduced the atro- phy of the triceps surae on the affected side and promoted the regeneration of nerve fibers of the damaged spinal cord at 8 weeks after injury. We conclude that puerarin exerts an ongoing role to activate growth-associated protein 43 in the corresponding segment of the spinal cord after sciat- ic nerve injury, thus contributing to neural regeneration after sciatic nerve injuries.
文摘Objective To investigate the significance of d ecompression of optic nerve in the re habilitation of traumatic optic neu ropathy following head trauma.Method Surgical treatment was performed on16patients suffered from traumatic neuropathy following head trau-ma.Fragments ,hematoma,necrotic b rain tissue resulting from fracture of orbital bone were removed and fron tal and orbital fracture or malpo-sition corrected by craniotomy thro ugh valve of frontal bone in 15patien ts.Optic canal was probed and sheath of optic nerve was cut open for decompression of optic nerve.Front otemporal wound was made in 1case with superior orbital fissure syndrome and decompression of optic canal and abrasion of superior orbital fis sure were performed through outer side of orbital point.Result Surgical treatment was effective in12cases(75%),exophthalmus in 2cases was corrected.Conclusion Decompression of optic nerve throug h frontal bone or frontotemporal is e ffective in enhancing visual acuity in patients with traumatic optic neuropathy following head trauma.
基金Supported by Scientific and Technological Projects of Anhui ProvinceNational Key Laboratory of Tea Biology and Resource Utilization of Anhui Agricultural University~~
文摘The planning of prior conservation areas is an important part of nature re- serve system, ：it is of great significance for biodiversity conservation. The methods of priority planning include hot-spots analysis, gap analysis, systematic conservation planning analysis and multi-criteria decision analysis. The key issues of priority area planning include the determination of the protect objects, the analyses of the protec- tion value and protection cost, the win-win development of protection and economy, and the changes of the management strategies of the protected areas. In this study, the features and research progress of different conservation methods were analyzed, and based on the discussion on the key issues of the planning of the protected areas, the application prospect of different methods was analyzed.
