Objective:To investigate the proteomic characteristics of overweight/obesity and related abnormal glucose and lipid metabolism caused by phlegm-dampness retention to identify related biomarkers.Methods:Seventy-one sub...Objective:To investigate the proteomic characteristics of overweight/obesity and related abnormal glucose and lipid metabolism caused by phlegm-dampness retention to identify related biomarkers.Methods:Seventy-one subjects were enrolled in the study.We assessed blood glucose,blood lipids,body mass index(BMI),and phlegm-dampness pattern,which was confirmed by a traditional Chinese medicine clinician.Of the participants,we included healthy participants with normal weight(NW,n=23),overweight/obese participants with normal metabolism(ONM,n=19),overweight/obese participants with pre-diabetes(OPD,n=12),and overweight/obese participants with marginally-elevated blood lipids(OML,n=17).Among them,the ONM,OPD,and OML groups were diagnosed with phlegmdampness pattern.The data-independent acquisition(DIA)method was first used to analyze the plasma protein expression of each group,and the relevant differential proteins of each group were screened.The co-expressed proteins were evaluated by Venn analysis.The pathway analyses of the differential proteins were analyzed using Ingenuity Pathway Analysis(IPA)software.Parallel reaction monitoring(PRM)was used to verify the differential and common proteins in each group.Results:After comparing ONM,OPD,and OML groups with NW group,we identified the differentially expressed proteins(DEPs).Next,we determined the DEPs among OPD,OML,and ONM groups.Using Venn analysis of the DEPs in each group,24 co-expressed proteins were screened.Two co-expressed proteins were verified by PRM.IPA analysis showed that pathways including LXR/RXR activation,acute phase response signaling,and FXR/RXR activation were common to all three groups of phlegmdamp overweight/obesity participants.However,the activation or inhibition of these pathways was different among the three groups.Conclusion:Participants with overweight/obesity have similar proteomic characteristics,though each type shows specific proteomic characteristics.Two co-expressed proteins,VTN and ORM1,are potential biomarkers for glucose and lipid metabolism diseases with overweight/obesity caused by phlegmdampness retention.展开更多
Background:Alterations in macular thickness and vascular density before clinically visible diabetic retinopathy(DR)remain inconclusive.This study aimed to determine whether retinal manifestations in abnormal glucose m...Background:Alterations in macular thickness and vascular density before clinically visible diabetic retinopathy(DR)remain inconclusive.This study aimed to determine whether retinal manifestations in abnormal glucose metabolism(AGM)patients differ from those in the healthy individuals.Methods:PubMed,Embase,and Web of Science were searched between 2000 and 2021.The eligibility criteria were AGM patients without DR.Primary and secondary outcomes measured by optical coherence tomography(OCT)and OCT angiography(OCTA)were analyzed and expressed as standardized mean differences(SMDs)with 95%confidence intervals(CIs).A random-effects model was used in the data synthesis.The potential publication bias for the variables was evaluated using Egger’s test.Results:A total of 86 observational studies involving 13,773 participants and 15,416 eyes were included.OCT revealed that compared to healthy controls,the total macular thickness of AGM patients was thinner,including the thickness of fovea(-0.24,95%CI[-0.39,-0.08];P=0.002,I^(2)=87.7%),all regions of parafovea(-0.32,95%CI[-0.54,-0.11];P=0.003;I^(2)=71.7%)and the four quadrants of perifovea;the thickness of peripapillary retinal nerve fiber layer(pRNFL),macular retinal nerve fiber layer(mRNFL),and ganglion cell layer(GCL)also decreased.OCTA indicated that the superficial and deep vascular density decreased,the foveal avascular zone(FAZ)area enlarged,and the acircularity index(AI)reduced in AGM individuals.Conclusions:Retinal thinning and microvascular lesions have occurred before the advent of clinically detectable DR;OCT and OCTA may have the potential to detect these preclinical changes.Registration:PROSPERO;http://www.crd.york.ac.uk/prospero/;No.CRD42021269885.展开更多
Objective To evaluate the association of fasting plasma glucose(FPG)level over 5.3 mmol/L with the development of abnormal glucose metabolism and cardiovascular disease(CVD).Methods This was a retrospec-tive cohort st...Objective To evaluate the association of fasting plasma glucose(FPG)level over 5.3 mmol/L with the development of abnormal glucose metabolism and cardiovascular disease(CVD).Methods This was a retrospec-tive cohort study with 1 064 non-diabetic subjects(980males;84 females)aged 60 or over,who carried out annual health check-up in Chinese PLA General展开更多
Background In this study, we aimed to evaluate the impact of abnormal glucose, lipid and Cystatin-C on the virtual P vector characteristics, which haven＇ t been reported in previous studies. Methods 204 of non-diabet...Background In this study, we aimed to evaluate the impact of abnormal glucose, lipid and Cystatin-C on the virtual P vector characteristics, which haven＇ t been reported in previous studies. Methods 204 of non-diabetes mellitus （NDM）, 130 of DM （type 2） and 39 of impaired glucose tolerance （IGT） patients were consecutively and retrospectively recruited. We selected a one-minute length of electrocardiogram at 4AM for analysis. After a series of calculating algorisms, we obtained the virtual planar P vector parameters. Results There were no significant differences in FPV, FPA, RSPV, RSPA, HPV and HPA groups. After adjusting confounding factors, the re- gression coefficients （RC） were estimated as follow： for FPV, female gender （RC -0.21, P = 0.02）, triglyceride （RC -0.09, P 〈 0.01）, RVOT （RC 0.03, P = 0.02）; for RSPV, female gender （RC -0.21, P 〈 0.01）, triglyceride （RC -0.10, P 〈 0.01）, average heart rate （RC 0.01, P = 0.02）; for HPV, triglyceride （RC -0.08, P 〈 0.001）, LDL （RC -0.19, P 〈 0.01）, Apo B （RC 0.67, P 〈 0.01）; for RSPA, B type of blood （RC -22.06, P = 0.02）, Cystatin-C （RC -72.79, P = 0.02）, thickness of interventricular septum （RC 3.70, P = 0.01）. Cystatin-C was suggested as a cure related to RSPA, and the cut-off point was 1.6 mg/L. There were no significant risk factors associated with FPA and HPA. There was no difference in virtual P vector among DM, IGT and NDM groups. Conclusion In- creased levels of lipid and Cystatin-C significantly impact the characteristics of virtual P vector, whereas glucose does not. These changes may come from a higher low voltage atrial area and abnormal orientation of atrial depolarization.展开更多
目的探讨复发性流产(RSA)与代谢性指标的关系。方法选取2021年8月1日至2022年6月25日太原市妇幼保健院生殖内分泌科门诊RSA女性患者60例为研究对象,同期招募月经规律、无流产史的妇女60例为对照组,采用体脂仪进行人体成分分析,测量血压...目的探讨复发性流产(RSA)与代谢性指标的关系。方法选取2021年8月1日至2022年6月25日太原市妇幼保健院生殖内分泌科门诊RSA女性患者60例为研究对象,同期招募月经规律、无流产史的妇女60例为对照组,采用体脂仪进行人体成分分析,测量血压并抽血测定甲状腺功能、同型半胱氨酸(Hcy)、尿酸、血脂水平以及肝功能、肾功能,并进行糖耐量试验和胰岛素释放试验;比较分析RSA与代谢性指标的可能相关性。结果RSA组与对照组两组患者间年龄、体质量指数(BMI)、体脂率、血脂水平、Hcy水平及尿酸水平等比较均无统计学差异(P>0.05);RSA组患者糖代谢异常的发生率显著高于对照组(90.0%vs.50.0%,P<0.001)。在RSA患者中,糖代谢异常组内脏脂肪面积增多(≥80 cm 2)者比例显著高于糖代谢正常组(61.1%vs.0%,P<0.05)。结论RSA女性患者无论肥胖与否,均易发生糖代谢异常,并且RSA患者的糖代谢异常与内脏脂肪面积增多相关。所以除了关注RSA女性的生育外,还要关注患者糖代谢的情况,同时需要进行生活方式干预,以减少内脏脂肪为主。展开更多
基金supported by the General Program of National Natural Science Foundation of China(81673836)。
文摘Objective:To investigate the proteomic characteristics of overweight/obesity and related abnormal glucose and lipid metabolism caused by phlegm-dampness retention to identify related biomarkers.Methods:Seventy-one subjects were enrolled in the study.We assessed blood glucose,blood lipids,body mass index(BMI),and phlegm-dampness pattern,which was confirmed by a traditional Chinese medicine clinician.Of the participants,we included healthy participants with normal weight(NW,n=23),overweight/obese participants with normal metabolism(ONM,n=19),overweight/obese participants with pre-diabetes(OPD,n=12),and overweight/obese participants with marginally-elevated blood lipids(OML,n=17).Among them,the ONM,OPD,and OML groups were diagnosed with phlegmdampness pattern.The data-independent acquisition(DIA)method was first used to analyze the plasma protein expression of each group,and the relevant differential proteins of each group were screened.The co-expressed proteins were evaluated by Venn analysis.The pathway analyses of the differential proteins were analyzed using Ingenuity Pathway Analysis(IPA)software.Parallel reaction monitoring(PRM)was used to verify the differential and common proteins in each group.Results:After comparing ONM,OPD,and OML groups with NW group,we identified the differentially expressed proteins(DEPs).Next,we determined the DEPs among OPD,OML,and ONM groups.Using Venn analysis of the DEPs in each group,24 co-expressed proteins were screened.Two co-expressed proteins were verified by PRM.IPA analysis showed that pathways including LXR/RXR activation,acute phase response signaling,and FXR/RXR activation were common to all three groups of phlegmdamp overweight/obesity participants.However,the activation or inhibition of these pathways was different among the three groups.Conclusion:Participants with overweight/obesity have similar proteomic characteristics,though each type shows specific proteomic characteristics.Two co-expressed proteins,VTN and ORM1,are potential biomarkers for glucose and lipid metabolism diseases with overweight/obesity caused by phlegmdampness retention.
基金supported by the National Natural Science Foundation of China(81870665,82171063 to DC)the Natural Science Foundation of Sichuan Province(2022NSFSC1285 to XR).
文摘Background:Alterations in macular thickness and vascular density before clinically visible diabetic retinopathy(DR)remain inconclusive.This study aimed to determine whether retinal manifestations in abnormal glucose metabolism(AGM)patients differ from those in the healthy individuals.Methods:PubMed,Embase,and Web of Science were searched between 2000 and 2021.The eligibility criteria were AGM patients without DR.Primary and secondary outcomes measured by optical coherence tomography(OCT)and OCT angiography(OCTA)were analyzed and expressed as standardized mean differences(SMDs)with 95%confidence intervals(CIs).A random-effects model was used in the data synthesis.The potential publication bias for the variables was evaluated using Egger’s test.Results:A total of 86 observational studies involving 13,773 participants and 15,416 eyes were included.OCT revealed that compared to healthy controls,the total macular thickness of AGM patients was thinner,including the thickness of fovea(-0.24,95%CI[-0.39,-0.08];P=0.002,I^(2)=87.7%),all regions of parafovea(-0.32,95%CI[-0.54,-0.11];P=0.003;I^(2)=71.7%)and the four quadrants of perifovea;the thickness of peripapillary retinal nerve fiber layer(pRNFL),macular retinal nerve fiber layer(mRNFL),and ganglion cell layer(GCL)also decreased.OCTA indicated that the superficial and deep vascular density decreased,the foveal avascular zone(FAZ)area enlarged,and the acircularity index(AI)reduced in AGM individuals.Conclusions:Retinal thinning and microvascular lesions have occurred before the advent of clinically detectable DR;OCT and OCTA may have the potential to detect these preclinical changes.Registration:PROSPERO;http://www.crd.york.ac.uk/prospero/;No.CRD42021269885.
文摘Objective To evaluate the association of fasting plasma glucose(FPG)level over 5.3 mmol/L with the development of abnormal glucose metabolism and cardiovascular disease(CVD).Methods This was a retrospec-tive cohort study with 1 064 non-diabetic subjects(980males;84 females)aged 60 or over,who carried out annual health check-up in Chinese PLA General
文摘Background In this study, we aimed to evaluate the impact of abnormal glucose, lipid and Cystatin-C on the virtual P vector characteristics, which haven＇ t been reported in previous studies. Methods 204 of non-diabetes mellitus （NDM）, 130 of DM （type 2） and 39 of impaired glucose tolerance （IGT） patients were consecutively and retrospectively recruited. We selected a one-minute length of electrocardiogram at 4AM for analysis. After a series of calculating algorisms, we obtained the virtual planar P vector parameters. Results There were no significant differences in FPV, FPA, RSPV, RSPA, HPV and HPA groups. After adjusting confounding factors, the re- gression coefficients （RC） were estimated as follow： for FPV, female gender （RC -0.21, P = 0.02）, triglyceride （RC -0.09, P 〈 0.01）, RVOT （RC 0.03, P = 0.02）; for RSPV, female gender （RC -0.21, P 〈 0.01）, triglyceride （RC -0.10, P 〈 0.01）, average heart rate （RC 0.01, P = 0.02）; for HPV, triglyceride （RC -0.08, P 〈 0.001）, LDL （RC -0.19, P 〈 0.01）, Apo B （RC 0.67, P 〈 0.01）; for RSPA, B type of blood （RC -22.06, P = 0.02）, Cystatin-C （RC -72.79, P = 0.02）, thickness of interventricular septum （RC 3.70, P = 0.01）. Cystatin-C was suggested as a cure related to RSPA, and the cut-off point was 1.6 mg/L. There were no significant risk factors associated with FPA and HPA. There was no difference in virtual P vector among DM, IGT and NDM groups. Conclusion In- creased levels of lipid and Cystatin-C significantly impact the characteristics of virtual P vector, whereas glucose does not. These changes may come from a higher low voltage atrial area and abnormal orientation of atrial depolarization.
文摘目的探讨复发性流产(RSA)与代谢性指标的关系。方法选取2021年8月1日至2022年6月25日太原市妇幼保健院生殖内分泌科门诊RSA女性患者60例为研究对象,同期招募月经规律、无流产史的妇女60例为对照组,采用体脂仪进行人体成分分析,测量血压并抽血测定甲状腺功能、同型半胱氨酸(Hcy)、尿酸、血脂水平以及肝功能、肾功能,并进行糖耐量试验和胰岛素释放试验;比较分析RSA与代谢性指标的可能相关性。结果RSA组与对照组两组患者间年龄、体质量指数(BMI)、体脂率、血脂水平、Hcy水平及尿酸水平等比较均无统计学差异(P>0.05);RSA组患者糖代谢异常的发生率显著高于对照组(90.0%vs.50.0%,P<0.001)。在RSA患者中,糖代谢异常组内脏脂肪面积增多(≥80 cm 2)者比例显著高于糖代谢正常组(61.1%vs.0%,P<0.05)。结论RSA女性患者无论肥胖与否,均易发生糖代谢异常,并且RSA患者的糖代谢异常与内脏脂肪面积增多相关。所以除了关注RSA女性的生育外,还要关注患者糖代谢的情况,同时需要进行生活方式干预,以减少内脏脂肪为主。
