Responses of Nitrogen Metabolism to Rhizobium Inoculation in Abrus mollis Hance

In different periods, the contents of nitrogen, ammonia nitrogen and nitrate nitrogen in different parts of Abrus mollis Hance were determined for each period by nesslerization, ninhydrin method and salicylic acid method in pot experiment. By using related data statistical software, the effects of Rhizobium inoculation on nitrogen metabolism, accumulation and distribution in Abrus mollis Hance were analyzed preliminarily.

Evaluation of Genotoxicity of Abrus mollis Hance with Single-cell Gel Electrophoresis Assay

[ Objective] This study aimed to evaluate the genotoxicity ofAbrus mollis Hance by using single cell gel electrophoresis. [Method] Forty mice were di- vided into five groups randomly, including positive control group （ cyclophosphamide group ）, negative control group （ physiological saline group）, high-dose A. moles Hance group （30 g/kg）, moderate-dose A. mollis Hance group （20 g/kg） and low-dose A. mollis Hance group （10 g/kg）. Tail DNA% and Tail Moment of mouse liver, kidney, lung and testicular cells were analyzed by using single cell gel electrophoresis assay, to investigate the effect of A. mollis Hance on DNA in mouse cells. [Result] Compared with positive control group, Tail DNA% and Tail Moment of moose liver, kidney, lung and testicular cells in A. moles Hance groups were significantly lower （ P 〈 0.01 ）. Compared with negative control group, Tail DNA% and Tail Moment of mouse liver, kidney, lung and testicular ceils in high-dose A. mollis Hance group were significantly lower （ P 〈 0.01 ）, while the other A. mollis Hance groups showed no statistically significant difference （ P 〉0.05 ）. [ Conclusion] A. mollis Hance has no damage effect on DNA in mouse cells within this experimental dose range.

Anti-inflammatory and hepatoprotective effects of total flavonoid C-glycosides from Abrus mollis extracts

The aim of this study was to evaluate the anti-inflammatory and hepatoprotective effects of the total flavonoid C-glycosides isolated from Abrus mollis extracts(AME). In the anti-inflammatory tests, xylene-induced ear edema model in mice and carrageenan-induced paw edema model in rats were applied. The hepatoprotective effects of AME were evaluated with various in vivo models of acute and chronic liver injury, including carbon tetrachloride(CCl4)-induced hepatitis in mice, D-galactosamine(D-GalN)-induced hepatitis in rats, as well as CCl4-induced hepatic fibrosis in rats. In the acute inflammation experiment, AME significantly suppressed xylene-induced ear edema and carrageenan-induced paw edema, respectively. In the acute hepatitis tests, AME significantly attenuated the excessive release of ALT and AST induced by CCl4 and D-GalN. In CCl4-induced hepatic fibrosis model, AME alleviated liver injury induced by CCl4 shown by histopathological sections of livers and improved liver function as indicated by decreased liver index, serum ALT, AST, TBIL, and ALP levels and hydroxyproline contents in liver tissues, and increased serum ALB and GLU levels. These results indicated that AME possesses potent anti-inflammatory activity in acute inflammation models and hepatoprotective activity in both acute and chronic liver injury models. In conclusion, AME is a potential anti-inflammatory and hepatoprotective agent and a viable candidate for treating inflammation, hepatitis, and hepatic fibrosis.