Based on the situation and progress of marine oil/gas exploration in the Sichuan Basin,SW China,the whole petroleum system is divided for marine carbonate rocks of the basin according to the combinations of hydrocarbo...Based on the situation and progress of marine oil/gas exploration in the Sichuan Basin,SW China,the whole petroleum system is divided for marine carbonate rocks of the basin according to the combinations of hydrocarbon accumulation elements,especially the source rock.The hydrocarbon accumulation characteristics of each whole petroleum system are analyzed,the patterns of integrated conventional and unconventional hydrocarbon accumulation are summarized,and the favorable exploration targets are proposed.Under the control of multiple extensional-convergent tectonic cycles,the marine carbonate rocks of the Sichuan Basin contain three sets of regional source rocks and three sets of regional cap rocks,and can be divided into the Cambrian,Silurian and Permian whole petroleum systems.These whole petroleum systems present mainly independent hydrocarbon accumulation,containing natural gas of affinity individually.Locally,large fault zones run through multiple whole petroleum systems,forming a fault-controlled complex whole petroleum system.The hydrocarbon accumulation sequence of continental shelf facies shale gas accumulation,marginal platform facies-controlled gas reservoirs,and intra-platform fault-and facies-controlled gas reservoirs is common in the whole petroleum system,with a stereoscopic accumulation and orderly distribution pattern.High-quality source rock is fundamental to the formation of large gas fields,and natural gas in a whole petroleum system is generally enriched near and within the source rocks.The development and maintenance of large-scale reservoirs are essential for natural gas enrichment,multiple sources,oil and gas transformation,and dynamic adjustment are the characteristics of marine petroleum accumulation,and good preservation conditions are critical to natural gas accumulation.Large-scale marginal-platform reef-bank facies zones,deep shale gas,and large-scale lithological complexes related to source-connected faults are future marine hydrocarbon exploration targets in the Sichuan Basin.展开更多
[Objectives]This study was conducted to investigate the effects of slow-release nitrogen fertilizer on dry matter accumulation and translocation of summer maize.[Methods]With Zhoudan 9 as the test variety,six differen...[Objectives]This study was conducted to investigate the effects of slow-release nitrogen fertilizer on dry matter accumulation and translocation of summer maize.[Methods]With Zhoudan 9 as the test variety,six different treatment were set up:blank control(CK1),slow-release urea 75 kg/hm^(2)(C1),slow-release urea 150 kg/hm^(2)(C2),slow-release urea 225 kg/hm^(2)(C3),slow-release urea 300 kg/hm^(2)(C4)and ordinary urea 300 kg/hm^(2)(CK2),to study the change law of dry matter accumulation and translocation in summer maize.[Results]Treatment slow-release urea 225 kg/hm^(2)(C4)showed summer maize yield,dry matter translocation between organs,grain contribution rate and proportion of grain dry matter in the full ripe stage higher than other treatments.Considering the weight loss and cost factors,slow-release urea 225 kg/hm^(2)(C3)could be recommended as the fertilizing amount for summer maize.[Conclusions]This study provides theoretical reference for rational selection of fertilizers for reducing fertilizer application and increasing fertilizer efficiency,and for production of summer maize in Shajiang black soil region.展开更多
[Objective] To understand the effect of nitrogen application on dry matter accumulation and allocation dynamics in broomcorn millet. [Method] The accumulation and distribution of dry matter were studied using cultivar...[Objective] To understand the effect of nitrogen application on dry matter accumulation and allocation dynamics in broomcorn millet. [Method] The accumulation and distribution of dry matter were studied using cultivars Jin Shu 7 and Huang Mizi at different levels of nitrogen fertilizer at the jointing stage. [Result] The results showed that increasing N application led to the increase of green leaf area and the delay of leaf senescence, which was beneficial to the accumulation of dry matter.Appropriate nitrogen application(90 kg/hm2) could coordinate the translocation rate of dry matter among different plant parts, thereby enhancing the yield of broomcorn millet; among different organs, the contribution rate of stem to kernel was greater than that of leaf to kernel; there was obvious correlation between dry matter and yield. For Jin Shu 7, leaf area and dry weight of spike showed significant negative correlation with yield. [Conclusion] The formation of grain yield of broomcorn millet involved the accumulation and allocation of dry matter, the appropriate amount of nitrogen application(90 kg/hm2) could improve the rates of translocation and contribution of dry matter, thereby promoting the yield of broomcorn millet.展开更多
Field experiments were conducted in farmers’ rice fields in 2001 and 2002 to study the effects of nitrogen (N) man-agement strategies on N use efficiency in recovery (RE), agronomy (AE) and physiology (PE) and redist...Field experiments were conducted in farmers’ rice fields in 2001 and 2002 to study the effects of nitrogen (N) man-agement strategies on N use efficiency in recovery (RE), agronomy (AE) and physiology (PE) and redistribution of dry matter accumulation (DMA) and nitrogen accumulation (NA) in two typical rice cultivars in Jinhua, Zhejiang Province. This study aimed mainly at identifying the possible causes of poor fertilizer N use efficiency (NUE) of rice in Zhejiang by comparing farmers’ fertilizer practice (FFP) with advanced site-specific nutrient management (SSNM) and real-time N management (RTNM). The results showed that compared to FFP, SSNM and RTNM reduced DMA and NA before panicle initiation and increased DMA and NA at post-flowering. There is no significant difference between SSNM and FFP in post-flowering dry matter redistribution (post-DMR) and post-flowering nitrogen redistribution (post-NR). These results suggest that high input rate of fertilizer N and improper fertilizer N timing are the main factors causing low NUE of irrigated rice in the farmer’s routine practice of Zhejiang. With SSNM, about 15% of the current total N input in direct-seeding early rice and 45% in single rice could be reduced without yield loss in Zhejiang, China.展开更多
[Objective] The aim of the research was to find the optimal nitrogen application rate, density and seedling age for no-tillage rape in seedling stage. [Method] With the D-optimal quadratic regression design for three ...[Objective] The aim of the research was to find the optimal nitrogen application rate, density and seedling age for no-tillage rape in seedling stage. [Method] With the D-optimal quadratic regression design for three factors, the 310 scheme was designed to study the effects of nitrogen application rate, density and seedling age on dry matter accumulation of no-tillage rape in seedling stage. [Result] With the increase of nitrogen application rate, density and seedling age, the dry matter content appeared like a parabola, increasing firstly and then declining. The change of nitrogen application rate caused greater influence than that of density and seedling age; the interaction effects between nitrogen application rate and density were greater than that between nitrogen application rate and seedling age as well as between density and seedling age. [Conclusion] Considered comprehensively, the dry matter content of no-tillage rape in seedling stage reached the highest level (4 768.2 kg/hm2) when the nitrogen application rate, the density and the seedling age were 195 kg/hm2, 93 000 plants/hm2 and 33 d, respectively.展开更多
[Objective] This assay was to explore the contribution of enzymes ac- counting for nitrogen reutilization in two Brassica napus varieties No.6 (low nitrogen use efficiency) and No.2 (high nitrogen use efficiency)....[Objective] This assay was to explore the contribution of enzymes ac- counting for nitrogen reutilization in two Brassica napus varieties No.6 (low nitrogen use efficiency) and No.2 (high nitrogen use efficiency). [Method] We measured the yield, transportation and accumulation of grain nitrogen, loss of leaf nitrogen and ni- trogen use efficiency (NUE) in the two rape varieties, by inhibiting proteolytic en- zyme (PE), glutamine synthetase (GS) and glutamate synthetase (GOGAT) and la- beling with lSN. [Result] Under GOGAT inhibitor treatment, both of the two varieties presented minimum NUE, yield and nitrogen transportation in grain and maximum ni- trogen loss in leaf. The effect of PE inhibitor was the second greatest, and that of GS inhibitor was the lowest. Moreover, 80% of the nitrogen that had been accumu- lated in leaf was transported out during late growth stage, and 50% to 70% of the grain nitrogen derived from the nitrogen in vegetative organs. The two varieties ex- hibited the same tendency. Rape variety No.2 had higher yield, grain nitrogen accu- mulation and lower nitrogen loss compared with No.6. [Conclusion] GOGAT has the greatest effect on the accumulation of grain nitrogen, yield and nitrogen reutilization in rape. Different enzyme activity may be the major factor resulting in different nitro- gen rautilization in the two rape varieties. Nitrogen stored in leaf during early growth stage is mainly used for nitrogen remobilization. A large proportion of grain nitrogen derives from vegetative tissues.展开更多
Deep placement of controlled-release fertilizer increases nitrogen (N) use efficiency in rice planting but is expensive. Few studies on direct-seeded rice have examined the effects of deep placement of conventional fe...Deep placement of controlled-release fertilizer increases nitrogen (N) use efficiency in rice planting but is expensive. Few studies on direct-seeded rice have examined the effects of deep placement of conventional fertilizer. With prilled urea serving as N fertilizer, a two-year field experiment with two N rates (120 and 195 kg/hm2) and four basal N application treatments (B50, all fertilizer was broadcast with 50% as basal N;D50, D70 and D100 corresponded to 50%, 70% and 100% of N deeply placed as basal N, respectively) were conducted in direct-seeded rice in 2013 and 2014. Soil N distribution and plant N uptake were analyzed. The results showed that deep placement of basal N significantly increased total N concentrations in soil. Significantly greater soil N concentrations were observed in D100 compared with B50 at 0, 6 and 12 cm (lateral distance) from the fertilizer application point both at mid-tillering and heading stages. D100 presented the highest values of dry matter and N accumulation from seeding to mid-tillering stages, but it presented the lowest values from heading to maturity stages and the lowest grain yield for no sufficient N supply at the reproductive stage. The grain yield of D50 was the highest, however, no significant difference was observed in grain yield, N agronomic efficiency or N recovery efficiency between D70 and D50, or between D70 and B50, while D70 was more labor saving than D50 for only one topdressing was applied in D70 compared with twice in other treatments. The above results indicated that 70% of fertilizer-N deeply placed as a basal fertilizer and 30% of fertilizer-N topdressed as a panicle fertilizer constituted an ideal approach for direct-seeded rice. This recommendation was further verified through on-farm demonstration experiments in 2015, in which D70 produced in similar grain yields as B50 did.展开更多
Utilization of organic nitrogen (N) is an important aspect of plant N assimilation and has potential application in sustainable agriculture. The aim of this study was to investigate the plant growth, C and N accumul...Utilization of organic nitrogen (N) is an important aspect of plant N assimilation and has potential application in sustainable agriculture. The aim of this study was to investigate the plant growth, C and N accumulation in leaves and roots of tomato seedlings in response to inorganic (NH4^+-N, NO3^-N) and organic nitrogen (Gly-N). Different forms of nitrogen (NH4^+-N, NO3^--N, Gly-N) were supplied to two tomato cultivars (Shenfen 918 and Huying 932) using a hydroponics system. The plant dry biomass, chlorophyll content, root activity, total carbon and nitrogen content in roots and leaves, and total N absorption, etc. were assayed during the cultivation. Our results showed that no significant differences in plant height, dry biomass, and total N content were found within the first 16 d among three treatments; however, significant differences in treatments on 24 d and 32 d were observed, and the order was NO3^--N 〉 Gly-N 〉 NH4^+-N. Significant differences were also observed between the two tomato cultivars. Chlorophyll contents in the two cultivars were significantly increased by the Gly-N treatment, and root activity showed a significant decrease in NHa^+-N treatment. Tomato leaf total carbon content was slightly affected by different N forms; however, total carbon in root and total nitrogen in root and leaf were promoted significantly by inorganic and organic N. Among the applied N forms, the increasing effects of the NH4^+-N treatment were larger than that of the Gly-N. In a word, different N resources resulted in different physiological effects in tomatoes. Organic nitrogen (e.g., Gly-N) can be a proper resource of plant N nutrition. Tomatoes of different genotypes had different responses under organic nitrogen (e.g., Gly-N) supplies.展开更多
Dissolved oxygen and nitrogen form have important effects on rice root growth and nitrogen availability.An indica hybrid rice,Guodao 1,and a conventional japonica rice,Xiushui 09,were cultured in hypoxic nutrient solu...Dissolved oxygen and nitrogen form have important effects on rice root growth and nitrogen availability.An indica hybrid rice,Guodao 1,and a conventional japonica rice,Xiushui 09,were cultured in hypoxic nutrient solution with NH4NO3 or(NH4)2SO4 as the nitrogen source for six weeks in pools.A portion of the Guodao 1 seedlings after treatment in the pools for four weeks were transferred to a split-root system at different dissolved oxygen contents and cultured for an additional two weeks.Biomass,root morphological traits and nitrogen accumulation were recorded.Under the low rhizosphere dissolved oxygen content(0-1.0 mg/L),plant biomass was significantly increased under NH4NO3-N supply by about 69% in Guodao 1 and 41% in Xiushui 09 compared with those under NH4+-N alone.Similar results were observed for root number,maximum root length,root dry weight and root activity.Nitrogen accumulations in roots and shoots were increased by 60% and 52% for Guodao 1,and by 41% and 33% for Xiushui 09,respectively,in the NH4NO3-N treatment.In the split-root system,the high rhizosphere dissolved oxygen content(8.0-9.0 mg/L) promoted root growth and development.Root biomass was increased by 21.6%,root number by 27%,maximum root length by 14%,and root volume by 10%.Moreover,nitrogen accumulation in roots was increased by 11% under high rhizosphere oxygen conditions.In conclusion,enhanced dissolved oxygen content and combined ammonium-nitrate nitrogen source have positive effects on root growth and nitrogen accumulation of rice plants.展开更多
Zinc(Zn) is an important essential microelement for wheat.In order to study the characteristics of Zn absorption,accumulation and distribution in highly-yielding winter wheat(with a grain yield of 9 000 kg ha-1),f...Zinc(Zn) is an important essential microelement for wheat.In order to study the characteristics of Zn absorption,accumulation and distribution in highly-yielding winter wheat(with a grain yield of 9 000 kg ha-1),field experiments were conducted in Gaocheng County of Hebei Province,China.Four winter wheat cultivars,i.e.,Shimai 14,Jifeng 703,Shimai 12,and Shixin 828,and four cultivars,i.e.,Temai 1,Shimai 12,Shixin 531,and Shixin 828,were used in the experiment,during 2004-2005 and 2005-2006,respectively.Plant samples were taken from the plots at each growing stage for Zn concentration analysis.The main results showed that the concentration of Zn in various above-ground organs of wheat was 9.5-112.5 mg kg-1 at different growing stages.The organ with the highest Zn concentration differed with the change of growth center at different growing stages.Accumulation of Zn in leaf blades was the highest among all the organs during early growing period,and more than 50% of the Zn accumulation was distributed to leaf blades before jointing,and higher than that to other organs.In late growing period,however,the accumulation of Zn in grains was the highest,and 58.1% of the Zn accumulation was distributed in grains at maturity.The total accumulation of Zn in wheat plant during its life span ranged from 384.9 to 475.9 g ha-1.The amount of Zn required for the formation of 100 kg grain yield ranged from 4.3 to 5.2 g.All the organs were ordered in such a sequence that leaf blades 〉 spikes 〉 leaf sheaths 〉 stems according to their net absorption and transportation of Zn as well as their contribution to Zn accumulation in grains.58.2-60.3% of the Zn accumulated in grains was redistributed from other organs,mostly from leaf blades.Concentration and accumulation of Zn in all the organs of wheat was high during early and middle growing periods,while accumulation of Zn in grains during late growing period mainly depended on the redistribution from other organs.According to these characteristics of Zn absorption and accumulation,Zn should be applied as seed dressing or basal fertilizer,so as to accelerate the early growth and Zn absorption of wheat.展开更多
Distribution and accumulation of Nd, and its effect on secretion of progesterone in mice were studied using radioisotope tracer ((()^(147)Nd)) technique. Following single intraperitoneal administration of neodymium tr...Distribution and accumulation of Nd, and its effect on secretion of progesterone in mice were studied using radioisotope tracer ((()^(147)Nd)) technique. Following single intraperitoneal administration of neodymium traced with (()^(147)Nd) at a dose of 200 mg·kg^(-1), uneven distribution of the radioactive Nd occurred in various tissues and organs. Much amount of (()^(147)Nd) accumulates in the bone, and the residue increases with the lapse of time. Some amount of radioactivity was also detected in eyes, blood and brain, but the accumulation decreased with the time due to excretion and re-distribution in mice. In comparison with controls, concentration of progesterone is found to be significantly lower in the serum of administered mice, indicating a significantly inhibitory effect of Nd on secretion of progesterone.展开更多
Based on split plot design method of field test,the impacts of supplemental irrigation based on soil moisture measurement and nitrogen use on winter wheat yield and nitrogen absorption and distribution were studied.Su...Based on split plot design method of field test,the impacts of supplemental irrigation based on soil moisture measurement and nitrogen use on winter wheat yield and nitrogen absorption and distribution were studied.Supplemental irrigation had three levels: 60%(W_1),70%(W_2) and 80%(W3) of the targeted relative water content at 0-40 cm of soil layer during jointing period of winter wheat.Nitrogen fertilization had three levels: not using nitrogen(N_0),using pure nitrogen of 195 kg/hm^2(N_(195)) and 255 kg/hm^2(N_(255)).Results showed that:(i)different supplemental irrigation and nitrogen fertilization significantly affected plant height and leaf area of winter wheat during key growth period.Under the same supplemental irrigation treatment,both plant height and leaf area of winter wheat showed as N_(255)> N_(195)> N_0(P <0.05).Plant height in N_(195) and N_(255)treatments was significantly higher than that in N_0 treatment,but there was not significant difference between N_(195) and N_(255)(P >0.05).Under the same nitrogen fertilization,plant height in W_2(569.4 m^3/hm^2) and W3(873.45 m^3/hm^2) treatments was significant higher than that in W_1(265.2 m^3/hm^2),but there was not significant difference between W_2 and W3(P >0.05).It illustrated that excessive nitrogen fertilization and supplemental irrigation did not significantly affect plant height and leaf area of winter wheat.(ii) Under the same nitrogen fertilization level,yield increase effect of winter wheat by supplemental irrigation showed a declining trend with nitrogen application amount increased.It illustrated that nitrogen fertilization and supplemental irrigation had certain critical values on the yield of winter wheat.When surpassing the critical value,the yield declined.When nitrogen fertilization amount was 195 kg/hm^2,and supplemental irrigation amount was 70% of field moisture capacity(569.4 m^3/hm^2),the highest yield 8500 kg/hm^2 could be obtained.(iii) During mature period of winter wheat,nitrogen accumulation amount of plant treated by nitrogen was significantly higher than that not treated by nitrogen(P <0.05).But under the treatments of W_2 and W3,nitrogen accumulation amount in N_(255) significantly declined when compared with N_(195)(P <0.05).Especially under W3(873.45 m^3/hm^2) level,nitrogen accumulation amount in N_(255) was even lower than N_0.Under the treatments of N_0 and N_(195),nitrogen accumulation amount of plant significantly increased with supplemental irrigation increased(P < 0.05).But under N_(255) treatment,there was not significant difference(P > 0.05).It illustrated that moderate supplemental irrigation and nitrogen fertilization could improve nitrogen absorption ability of winter wheat,but excessive supplemental irrigation and nitrogen fertilization were not favorable for plant's nitrogen absorption.(iv) Although the increase of supplemental irrigation during jointing period improved nitrogen absorption ability of winter wheat and promoted winter wheat absorbing more nitrogen,it inhibited nitrogen transferring and distributing to seed.Comprehensively considering growth condition of winter wheat and nitrogen risk condition,it is suggested that nitrogen application amount was 195 kg/hm^2,and supplemental irrigation reached 70% of field moisture capacity(569.4 m^3/hm^2),which could be as the suitable water and fertilizer use amounts in the region.展开更多
[Objectives]This study was conducted to reveal the characteristics of nutrient absorption and accumulation in Pinus massoniana plantations in Northwestern Guangxi.[Methods]Based on field investigation and indoor analy...[Objectives]This study was conducted to reveal the characteristics of nutrient absorption and accumulation in Pinus massoniana plantations in Northwestern Guangxi.[Methods]Based on field investigation and indoor analysis,the contents,accumulation and annual net accumulation of five nutrient elements(N,P,K,Ca and Mg)in a mature P.massoniana plantation(26-year-old)in Nandan County,Guangxi Province were studied.[Results]The contents of nutrient elements in different organs of the mature P.massoniana plantation were the highest in the leaves,followed by the bark,branch and root,and the lowest in the stem.In general,among the contents of the five elements in different organs,N content was the highest,followed by K or Ca,and P and Mg were the lowest.The total accumulation of nutrient elements in the 26-year-old mature P.massoniana plantation in northwestern Guangxi was 1 384.05 kg/hm^2.Among the different structural levels of the stand,the tree layer had the highest accumulation of nutrient elements,which was 1 198.41 kg/hm^2,accounting for 86.59%of the total accumulation of nutrients in the plantation,and the accumulation of nutrients in other layers from the largest to the smallest was the litter layer(91.97 kg/hm^2),herb layer(49.86 kg/hm^2)and shrub layer(43.92 kg/hm^2),accounting for 3.17%,3.60%and 6.64%of the total nutrient accumulation of the plantation,respectively.The annual net accumulation of nutrient elements in the tree layer of the mature P.massoniana plantation was 46.09 kg/(hm^2·a),and the order of the annual net accumulation of different nutrient elements followed N>K>Ca>Mg>P;and the accumulation of 1 t of dry matter needed 6.37 kg of the five nutrients.[Conclusions]This study provides a scientific basis for the rational management of P.massoniana plantations,especially forest soil management.展开更多
Planting maize at high densities leads to early leaf senescence,and the resulting reduction in the number of lower leaves affects the plant’s root function and lowers its grain yield.However,the nature of the process...Planting maize at high densities leads to early leaf senescence,and the resulting reduction in the number of lower leaves affects the plant’s root function and lowers its grain yield.However,the nature of the process by which lower leaf senescence affects biomass accumulation and grain yield formation in maize is not clear.This study aimed to shed light on how these factors are related by investigating the effects of the plant growth regulator 6-benzyladenine(6-BA)on the senescence of lower leaves of maize plants.In two maize cultivars planted at densities of 67,500(low density,LD)and 90,000(high density,HD)plants ha^(-1),plants treated with 6-BA maintained a high green leaf area index(LAI)longer than control(CK)plants,enabling them to maintain a higher photosynthetic rate for a longer period of time and produce more biomass before reaching physiological maturity.Spraying the lower leaves of maize plants with a 6-BA solution increased the distribution of;C-photosynthates to their roots,lower leaves and bracts,a result that can be ascribed to a decreased retention of;C-photosynthates in the stem and grain.In both seasons of the experiment,maize plants treated with 6-BA accumulated more N in grain and maintained a higher N content in roots and leaves,especially in lower leaves,than CK.Increased C assimilation in the lower leaves may explain why N uptake in plants subjected to the 6-BA treatment exceeded that in CK plants and why both photosynthesis rate and dry matter accumulation were maintained throughout grain filling.Our results suggest that a suitable distribution of C and N in leaves post-silking may maintain plant root function,increase N use efficiency,maximize the duration of high LAI,and increase grain yield.展开更多
Microalgal lipids are regarded as main future feedstock of biofuels for its higher efficiency of accumulation and sus- tainable production. In order to investigate the effect of various nitrogen to phosphorus ratios o...Microalgal lipids are regarded as main future feedstock of biofuels for its higher efficiency of accumulation and sus- tainable production. In order to investigate the effect of various nitrogen to phosphorus ratios on cells growth, chlorophyll content and accumulation of lipids in Dunaliella tertiolecta, experiments were carried out in modified microalgal medium with inorganic nitrogen (nitrate-nitrogen) or organic nitrogen (urea-nitrogen) as the sole nitrogen source at initial N:P ratios ranging from 1:1 to 32:1. The favorable N:P of 16:1 in the nitrate-N or urea-N medium yielded the maximum cell density and specific growth rate. Decrease in chlorophyll content were observed at the N:P of 4:1 in both nitrate-N and urea-N cultures. It was also observed that the maximum lipids concentration was obtained at the N:P of 4:1 in both nitrate and urea nutrient medium. The lipid productivity and lipid content of cultures in the urea-N medium at the N:P of 4: lwere markedly higher than those from cultures with other N:P ratios (p〈 0.05). The results of this work illustrate the possibility that higher ratios of nitrogen to phosphorus have enhancing effect on cells growth of D. tertiolecta. Conversely, higher lipid accumulation is associated with a decrease in chlorophyll content under lower ratios of nitro- gen to phosphorus. The results confirm the hypothesis of this study that a larger metabolic flux has been channeled to lipid accumu- lation in D. tertiolecta cells when the ratios of nitrogen to phosphorus drop below a critical level.展开更多
The leaf photosynthesis and nitrogen(N) translocation in three large-spike lines and control cultivar(Xi'nong 979) of winter wheat(Triticum aestivum L.) were studied in 2010–2011 and 2011–2012. The objectives...The leaf photosynthesis and nitrogen(N) translocation in three large-spike lines and control cultivar(Xi'nong 979) of winter wheat(Triticum aestivum L.) were studied in 2010–2011 and 2011–2012. The objectives of this study were to investigate the differences in the physiological characteristics of large-spike lines and control cultivar and identify the limiting factors that play a role in improving the yield of breeding materials. The average yield, grain number per spike, kernel weight per spike, and 1 000-kernel weight of the large-spike lines were 16.0, 26.8, 42.6, and 15.4%, respectively, significantly higher than those of control. The average photosynthetic rates(Pn) were not significant between the large-spike lines and control cultivar during the active growth period. The average PSII maximum energy conversion efficiency(Fv/Fm), PSII actual quantum efficiency(Ф(PSII)), photochemical quenching coefficient(qP), PSII reaction center activity(Fv′/Fm′) and water-use efficiency(WUE) of the large-spike lines were 1.0, 5.1, 3.6, 0.8, and 43.4%, respectively, higher than those of the control during the active growth stages. The N distribution proportions in different tissues were ranked in the order of grains〉culms+sheathes〉rachis+glumes〉flag leaves〉penultimate leaves〉remain leaves. This study suggested that utilization of the large-spike wheat might be a promising approach to obtain higher grain yield in Northwest China.展开更多
It is very important to study eco-physiological processes of plants and to determine quantitative relations between accumulation, distribution of dry matter and environmental factors for regionalization, standardizati...It is very important to study eco-physiological processes of plants and to determine quantitative relations between accumulation, distribution of dry matter and environmental factors for regionalization, standardization and precision agriculture. Meanwhile, global changes, e.g., atmospheric CO2 concentration rising, global warming, and climate abnormity, have been effecting on agricultural productivity. This study provides a theoretical basis for predicting productive potentials and development trends in different agricultural regions. One-year-old black walnut (Juglans hindsii) seedlings were employed as subjects for setting up the dynamic models of dry matter accumulation and distribution, based on mechanistic models of photosynthesis, matter conservation and concentration gradient. Under optimum conditions of soil moisture and mineral nutrient, during the period of the canopy construction, the dry matter accumulation of the canopy conformed to logistic curves; but the accumulation of both total biornass and dry matter of stem-root could be divided into two phases: the first phase was exponential increase, the second was linear increase. The total biomass, dry matter of canopy and stem-root all presented a fluctuant increase, which was affected by the environmental factors. Ratio of daily increase of dry matter in the canopy and the steem-root (dWJdWs) was changeable along with growth periods and environmental factors. At the initial stage of the canopy forming, dW/dWs was larger, about 3.2 on average, which indicated that the photosynthetic product was mainly used to develop leaves; in the midterm, it was about 1.9, which indicated that the distribution of dry matter in the canopy and in the stem-root was relatively balanced; when the plant tended to stop growing, dWl/dWs decreased linearly, and the main distribution of dry matter moved to the roots.展开更多
With Qingyu 1 and Qingyu 2 as test materials, variation of soluble carbohydrate content in different parts were studied under two types of artificially simulated drought stress (polyethylene glycd PEG-6000 stress and...With Qingyu 1 and Qingyu 2 as test materials, variation of soluble carbohydrate content in different parts were studied under two types of artificially simulated drought stress (polyethylene glycd PEG-6000 stress and water-controlling stress). The results showed that under the stress of PEG, soluble carbohydrate content in leaves of Qingyu 1 and Qingyu 2 increased with the prolonging time of stress; soluble carbohydrate content in stems of Qingyu 1 did not show regular changes with the prolonging time of stress, while 30% PEG stress received the best effect on Qingyu 2 after 24 h; soluble carbohydrate content in roots of Qingyu 1 and Qingyu 2 increased with the prolonging time of stress. Under different intensities of water-controUing stress, soluble carbohydrate content in leaves of Qingyu 1 first increased then decreased, while that in leaves of Qingyu 2 increased; soluble carbohydrate content in stems of Qingyu 1 and Qingyu 2 increased with the pro- longing time of water-controlling stress; soluble carbohydrate content in roots of Qingyu 1 decreased with the prolonging time of water-contmlling stress, while that in roots of Qingyu 2 differed slightly.展开更多
To study the accumulation regularity of nitrogen and phosphorus in typical constructive plants in coastal wetland,samples of Suaeda glauca(Bunge) Bunge,Phragmites austrahs and Tamarix chinensis Lour,were taken from th...To study the accumulation regularity of nitrogen and phosphorus in typical constructive plants in coastal wetland,samples of Suaeda glauca(Bunge) Bunge,Phragmites austrahs and Tamarix chinensis Lour,were taken from the Yellow River Delta National Coast Wetland Nature Reserve,nitrogen and phosphorus content in plants was measured and analyzed.The results showed that ① nitrogen and phosphorus content in different wetland plants is correlated;② different species in the same place and the same species in different spaces show different accumulation regularity of nitrogen and phosphorus;③ nitrogen and phosphorus content in plants is closely related to nitrogen and phosphorus content in the habitat;④ nitrogen content in T.chinensis Lour,is the highest,the mean is 11.63 g/kg,and phosphorus content in S glauca(Bunge) Bunge is the highest,the mean is 1.38 g/kg;⑤ nitrogen content in the 3 species:T.chinensis Lour.> S.glauca(Bunge) Bunge > P.australis;⑥ nitrogen content in aboveground parts of all plants is significantly higher than that in underground parts,and phosphorus content in aboveground parts of all plants except S.glauca(Bunge) Bunge is significantly higher than that in underground parts;⑦ nitrogen content in the 3 species in the study area is significantly higher than phosphorus content in these species.展开更多
Readily available chemical fertilizers have resulted in a decline in the use of organic manure(e.g.,green manures),a traditionally sustainable source of nutrients.Based on this,we applied urea at the rate of 270 kg ha...Readily available chemical fertilizers have resulted in a decline in the use of organic manure(e.g.,green manures),a traditionally sustainable source of nutrients.Based on this,we applied urea at the rate of 270 kg ha−1 with and without green manure in order to assess nitrogen(N)productivity in a double rice cropping system in 2017.In particular,treatment combinations were as follows:winter fallow rice-rice(WF-R-R),milk vetch rice-rice(MV-R-R),oil-seed rape rice-rice(R-R-R)and potato crop rice-rice(P-R-R).Results revealed that green manure significantly(p≤0.05)improved the soil chemical properties and net soil organic carbon content increased by an average 117.47%,total nitrogen(N)by 28.41%,available N by 26.64%,total phosphorus(P)by 37.77%,available P by 20.48%and available potassium(K)by 33.10%than WF-R-R,however pH was reduced by 3.30%across the seasons.Similarly,net dry matter accumulation rate enhanced in green manure applied treatments and ranked in order:P-R-R>R-R-R>MV-R-R>WF-R-R.Furthermore,the total leaf dry matter transport(t ha−1)for the P-R-R in both seasons was significantly higher by an average 11.2%,7.2%and 36%than MV-R-R,R-R-R,and WF-R-R,respectively.In addition,net total nitrogen accumulation(kg ha−1)was found higher in green manure applied plots compared to the control.Yield and yield attributed traits were observed maximum in green manure applied plots,with treatments ranking as follows:P-R-R>R-R-R>MV-R-R>WF-R-R.Thus,results obtained highlight ability of green manure to sustainably improve soil quality and rice yield.展开更多
基金Supported by the National Natural Science Foundation of China(42090022)。
文摘Based on the situation and progress of marine oil/gas exploration in the Sichuan Basin,SW China,the whole petroleum system is divided for marine carbonate rocks of the basin according to the combinations of hydrocarbon accumulation elements,especially the source rock.The hydrocarbon accumulation characteristics of each whole petroleum system are analyzed,the patterns of integrated conventional and unconventional hydrocarbon accumulation are summarized,and the favorable exploration targets are proposed.Under the control of multiple extensional-convergent tectonic cycles,the marine carbonate rocks of the Sichuan Basin contain three sets of regional source rocks and three sets of regional cap rocks,and can be divided into the Cambrian,Silurian and Permian whole petroleum systems.These whole petroleum systems present mainly independent hydrocarbon accumulation,containing natural gas of affinity individually.Locally,large fault zones run through multiple whole petroleum systems,forming a fault-controlled complex whole petroleum system.The hydrocarbon accumulation sequence of continental shelf facies shale gas accumulation,marginal platform facies-controlled gas reservoirs,and intra-platform fault-and facies-controlled gas reservoirs is common in the whole petroleum system,with a stereoscopic accumulation and orderly distribution pattern.High-quality source rock is fundamental to the formation of large gas fields,and natural gas in a whole petroleum system is generally enriched near and within the source rocks.The development and maintenance of large-scale reservoirs are essential for natural gas enrichment,multiple sources,oil and gas transformation,and dynamic adjustment are the characteristics of marine petroleum accumulation,and good preservation conditions are critical to natural gas accumulation.Large-scale marginal-platform reef-bank facies zones,deep shale gas,and large-scale lithological complexes related to source-connected faults are future marine hydrocarbon exploration targets in the Sichuan Basin.
文摘[Objectives]This study was conducted to investigate the effects of slow-release nitrogen fertilizer on dry matter accumulation and translocation of summer maize.[Methods]With Zhoudan 9 as the test variety,six different treatment were set up:blank control(CK1),slow-release urea 75 kg/hm^(2)(C1),slow-release urea 150 kg/hm^(2)(C2),slow-release urea 225 kg/hm^(2)(C3),slow-release urea 300 kg/hm^(2)(C4)and ordinary urea 300 kg/hm^(2)(CK2),to study the change law of dry matter accumulation and translocation in summer maize.[Results]Treatment slow-release urea 225 kg/hm^(2)(C4)showed summer maize yield,dry matter translocation between organs,grain contribution rate and proportion of grain dry matter in the full ripe stage higher than other treatments.Considering the weight loss and cost factors,slow-release urea 225 kg/hm^(2)(C3)could be recommended as the fertilizing amount for summer maize.[Conclusions]This study provides theoretical reference for rational selection of fertilizers for reducing fertilizer application and increasing fertilizer efficiency,and for production of summer maize in Shajiang black soil region.
基金Supported by the Earmarked Fund for China Agriculture Research System(CARS-07-12.5-A12)
文摘[Objective] To understand the effect of nitrogen application on dry matter accumulation and allocation dynamics in broomcorn millet. [Method] The accumulation and distribution of dry matter were studied using cultivars Jin Shu 7 and Huang Mizi at different levels of nitrogen fertilizer at the jointing stage. [Result] The results showed that increasing N application led to the increase of green leaf area and the delay of leaf senescence, which was beneficial to the accumulation of dry matter.Appropriate nitrogen application(90 kg/hm2) could coordinate the translocation rate of dry matter among different plant parts, thereby enhancing the yield of broomcorn millet; among different organs, the contribution rate of stem to kernel was greater than that of leaf to kernel; there was obvious correlation between dry matter and yield. For Jin Shu 7, leaf area and dry weight of spike showed significant negative correlation with yield. [Conclusion] The formation of grain yield of broomcorn millet involved the accumulation and allocation of dry matter, the appropriate amount of nitrogen application(90 kg/hm2) could improve the rates of translocation and contribution of dry matter, thereby promoting the yield of broomcorn millet.
基金Project supported by the International Rice Research Institute (IRRI)Swiss Agency for Development and Cooperation (SDC)+3 种基金the Potash & Phosphate Institute and the Potash & Phosphate Institute of Canada (PPI-PPIC)the International Fertilizer Industry Association (IFA)the International Potash Institute (IPI)948 Project of the Ministry of Agriculture of China (No. 2003-Z53)
文摘Field experiments were conducted in farmers’ rice fields in 2001 and 2002 to study the effects of nitrogen (N) man-agement strategies on N use efficiency in recovery (RE), agronomy (AE) and physiology (PE) and redistribution of dry matter accumulation (DMA) and nitrogen accumulation (NA) in two typical rice cultivars in Jinhua, Zhejiang Province. This study aimed mainly at identifying the possible causes of poor fertilizer N use efficiency (NUE) of rice in Zhejiang by comparing farmers’ fertilizer practice (FFP) with advanced site-specific nutrient management (SSNM) and real-time N management (RTNM). The results showed that compared to FFP, SSNM and RTNM reduced DMA and NA before panicle initiation and increased DMA and NA at post-flowering. There is no significant difference between SSNM and FFP in post-flowering dry matter redistribution (post-DMR) and post-flowering nitrogen redistribution (post-NR). These results suggest that high input rate of fertilizer N and improper fertilizer N timing are the main factors causing low NUE of irrigated rice in the farmer’s routine practice of Zhejiang. With SSNM, about 15% of the current total N input in direct-seeding early rice and 45% in single rice could be reduced without yield loss in Zhejiang, China.
基金Supported by the"11th Five-Year Plan"Significant Key Program of Guizhou Province[Guizhou Technology and Agriculture Co-word(2000)1109]Graduate Student Innovation Fund Project of Guizhou University[(2006)009]~~
文摘[Objective] The aim of the research was to find the optimal nitrogen application rate, density and seedling age for no-tillage rape in seedling stage. [Method] With the D-optimal quadratic regression design for three factors, the 310 scheme was designed to study the effects of nitrogen application rate, density and seedling age on dry matter accumulation of no-tillage rape in seedling stage. [Result] With the increase of nitrogen application rate, density and seedling age, the dry matter content appeared like a parabola, increasing firstly and then declining. The change of nitrogen application rate caused greater influence than that of density and seedling age; the interaction effects between nitrogen application rate and density were greater than that between nitrogen application rate and seedling age as well as between density and seedling age. [Conclusion] Considered comprehensively, the dry matter content of no-tillage rape in seedling stage reached the highest level (4 768.2 kg/hm2) when the nitrogen application rate, the density and the seedling age were 195 kg/hm2, 93 000 plants/hm2 and 33 d, respectively.
基金Supported by National Natural Science Foundation of China(31071851,31101596,31372130)National Key Technology Research and Development Program(2012BAD15B04,2010BAD01B01)+2 种基金Mordern Rape Industiral Technology SystemConstructionthe Innovation Platform Open Funds for Universities in Hunan Province(12K064,10K034)Special Fund from the People’s Government of Hunan Province(2012-45)~~
文摘[Objective] This assay was to explore the contribution of enzymes ac- counting for nitrogen reutilization in two Brassica napus varieties No.6 (low nitrogen use efficiency) and No.2 (high nitrogen use efficiency). [Method] We measured the yield, transportation and accumulation of grain nitrogen, loss of leaf nitrogen and ni- trogen use efficiency (NUE) in the two rape varieties, by inhibiting proteolytic en- zyme (PE), glutamine synthetase (GS) and glutamate synthetase (GOGAT) and la- beling with lSN. [Result] Under GOGAT inhibitor treatment, both of the two varieties presented minimum NUE, yield and nitrogen transportation in grain and maximum ni- trogen loss in leaf. The effect of PE inhibitor was the second greatest, and that of GS inhibitor was the lowest. Moreover, 80% of the nitrogen that had been accumu- lated in leaf was transported out during late growth stage, and 50% to 70% of the grain nitrogen derived from the nitrogen in vegetative organs. The two varieties ex- hibited the same tendency. Rape variety No.2 had higher yield, grain nitrogen accu- mulation and lower nitrogen loss compared with No.6. [Conclusion] GOGAT has the greatest effect on the accumulation of grain nitrogen, yield and nitrogen reutilization in rape. Different enzyme activity may be the major factor resulting in different nitro- gen rautilization in the two rape varieties. Nitrogen stored in leaf during early growth stage is mainly used for nitrogen remobilization. A large proportion of grain nitrogen derives from vegetative tissues.
基金supported by the National Key Research and Development Program of China(Grant No.2016YFD0300108)the National Natural Science Foundation of China(Grant Nos.31671630 and 31371581)the National Rice Industry Technology System(CARS-01-04A)in China
文摘Deep placement of controlled-release fertilizer increases nitrogen (N) use efficiency in rice planting but is expensive. Few studies on direct-seeded rice have examined the effects of deep placement of conventional fertilizer. With prilled urea serving as N fertilizer, a two-year field experiment with two N rates (120 and 195 kg/hm2) and four basal N application treatments (B50, all fertilizer was broadcast with 50% as basal N;D50, D70 and D100 corresponded to 50%, 70% and 100% of N deeply placed as basal N, respectively) were conducted in direct-seeded rice in 2013 and 2014. Soil N distribution and plant N uptake were analyzed. The results showed that deep placement of basal N significantly increased total N concentrations in soil. Significantly greater soil N concentrations were observed in D100 compared with B50 at 0, 6 and 12 cm (lateral distance) from the fertilizer application point both at mid-tillering and heading stages. D100 presented the highest values of dry matter and N accumulation from seeding to mid-tillering stages, but it presented the lowest values from heading to maturity stages and the lowest grain yield for no sufficient N supply at the reproductive stage. The grain yield of D50 was the highest, however, no significant difference was observed in grain yield, N agronomic efficiency or N recovery efficiency between D70 and D50, or between D70 and B50, while D70 was more labor saving than D50 for only one topdressing was applied in D70 compared with twice in other treatments. The above results indicated that 70% of fertilizer-N deeply placed as a basal fertilizer and 30% of fertilizer-N topdressed as a panicle fertilizer constituted an ideal approach for direct-seeded rice. This recommendation was further verified through on-farm demonstration experiments in 2015, in which D70 produced in similar grain yields as B50 did.
基金funded by the National High Technol-ogy Research and Development Program of China (863 Program,2006AA10Z221)China Postdoctoral Science Foundation (2005038436)+1 种基金Shanghai Leading Academic Discipline Project (B209)National Key Technologies R&D Program of China during the 11th Five-Year Plan period (2008BADA7B00 2008BADA7B01)
文摘Utilization of organic nitrogen (N) is an important aspect of plant N assimilation and has potential application in sustainable agriculture. The aim of this study was to investigate the plant growth, C and N accumulation in leaves and roots of tomato seedlings in response to inorganic (NH4^+-N, NO3^-N) and organic nitrogen (Gly-N). Different forms of nitrogen (NH4^+-N, NO3^--N, Gly-N) were supplied to two tomato cultivars (Shenfen 918 and Huying 932) using a hydroponics system. The plant dry biomass, chlorophyll content, root activity, total carbon and nitrogen content in roots and leaves, and total N absorption, etc. were assayed during the cultivation. Our results showed that no significant differences in plant height, dry biomass, and total N content were found within the first 16 d among three treatments; however, significant differences in treatments on 24 d and 32 d were observed, and the order was NO3^--N 〉 Gly-N 〉 NH4^+-N. Significant differences were also observed between the two tomato cultivars. Chlorophyll contents in the two cultivars were significantly increased by the Gly-N treatment, and root activity showed a significant decrease in NHa^+-N treatment. Tomato leaf total carbon content was slightly affected by different N forms; however, total carbon in root and total nitrogen in root and leaf were promoted significantly by inorganic and organic N. Among the applied N forms, the increasing effects of the NH4^+-N treatment were larger than that of the Gly-N. In a word, different N resources resulted in different physiological effects in tomatoes. Organic nitrogen (e.g., Gly-N) can be a proper resource of plant N nutrition. Tomatoes of different genotypes had different responses under organic nitrogen (e.g., Gly-N) supplies.
基金supported by the National Natural Science Foundation of China(Grant No.30571102)the Key Projects in the National Science & Technology Pillar Program of China(Grant No.2006BAD02A13)the Provincial Natural Science Foundation of Zhejiang(Grant No.Y3100270)
文摘Dissolved oxygen and nitrogen form have important effects on rice root growth and nitrogen availability.An indica hybrid rice,Guodao 1,and a conventional japonica rice,Xiushui 09,were cultured in hypoxic nutrient solution with NH4NO3 or(NH4)2SO4 as the nitrogen source for six weeks in pools.A portion of the Guodao 1 seedlings after treatment in the pools for four weeks were transferred to a split-root system at different dissolved oxygen contents and cultured for an additional two weeks.Biomass,root morphological traits and nitrogen accumulation were recorded.Under the low rhizosphere dissolved oxygen content(0-1.0 mg/L),plant biomass was significantly increased under NH4NO3-N supply by about 69% in Guodao 1 and 41% in Xiushui 09 compared with those under NH4+-N alone.Similar results were observed for root number,maximum root length,root dry weight and root activity.Nitrogen accumulations in roots and shoots were increased by 60% and 52% for Guodao 1,and by 41% and 33% for Xiushui 09,respectively,in the NH4NO3-N treatment.In the split-root system,the high rhizosphere dissolved oxygen content(8.0-9.0 mg/L) promoted root growth and development.Root biomass was increased by 21.6%,root number by 27%,maximum root length by 14%,and root volume by 10%.Moreover,nitrogen accumulation in roots was increased by 11% under high rhizosphere oxygen conditions.In conclusion,enhanced dissolved oxygen content and combined ammonium-nitrate nitrogen source have positive effects on root growth and nitrogen accumulation of rice plants.
基金supported by the Key Technologies R&D Program of China during the 11th Five-Year Plan period (2006BAD02A08)the Earmarked Fund for Modern Agro-Industry Technology Research System,China
文摘Zinc(Zn) is an important essential microelement for wheat.In order to study the characteristics of Zn absorption,accumulation and distribution in highly-yielding winter wheat(with a grain yield of 9 000 kg ha-1),field experiments were conducted in Gaocheng County of Hebei Province,China.Four winter wheat cultivars,i.e.,Shimai 14,Jifeng 703,Shimai 12,and Shixin 828,and four cultivars,i.e.,Temai 1,Shimai 12,Shixin 531,and Shixin 828,were used in the experiment,during 2004-2005 and 2005-2006,respectively.Plant samples were taken from the plots at each growing stage for Zn concentration analysis.The main results showed that the concentration of Zn in various above-ground organs of wheat was 9.5-112.5 mg kg-1 at different growing stages.The organ with the highest Zn concentration differed with the change of growth center at different growing stages.Accumulation of Zn in leaf blades was the highest among all the organs during early growing period,and more than 50% of the Zn accumulation was distributed to leaf blades before jointing,and higher than that to other organs.In late growing period,however,the accumulation of Zn in grains was the highest,and 58.1% of the Zn accumulation was distributed in grains at maturity.The total accumulation of Zn in wheat plant during its life span ranged from 384.9 to 475.9 g ha-1.The amount of Zn required for the formation of 100 kg grain yield ranged from 4.3 to 5.2 g.All the organs were ordered in such a sequence that leaf blades 〉 spikes 〉 leaf sheaths 〉 stems according to their net absorption and transportation of Zn as well as their contribution to Zn accumulation in grains.58.2-60.3% of the Zn accumulated in grains was redistributed from other organs,mostly from leaf blades.Concentration and accumulation of Zn in all the organs of wheat was high during early and middle growing periods,while accumulation of Zn in grains during late growing period mainly depended on the redistribution from other organs.According to these characteristics of Zn absorption and accumulation,Zn should be applied as seed dressing or basal fertilizer,so as to accelerate the early growth and Zn absorption of wheat.
文摘Distribution and accumulation of Nd, and its effect on secretion of progesterone in mice were studied using radioisotope tracer ((()^(147)Nd)) technique. Following single intraperitoneal administration of neodymium traced with (()^(147)Nd) at a dose of 200 mg·kg^(-1), uneven distribution of the radioactive Nd occurred in various tissues and organs. Much amount of (()^(147)Nd) accumulates in the bone, and the residue increases with the lapse of time. Some amount of radioactivity was also detected in eyes, blood and brain, but the accumulation decreased with the time due to excretion and re-distribution in mice. In comparison with controls, concentration of progesterone is found to be significantly lower in the serum of administered mice, indicating a significantly inhibitory effect of Nd on secretion of progesterone.
基金Supported by National Key Research Plan Project(2016YFD0801001,2016YFD0200103,2017YFD0800500)
文摘Based on split plot design method of field test,the impacts of supplemental irrigation based on soil moisture measurement and nitrogen use on winter wheat yield and nitrogen absorption and distribution were studied.Supplemental irrigation had three levels: 60%(W_1),70%(W_2) and 80%(W3) of the targeted relative water content at 0-40 cm of soil layer during jointing period of winter wheat.Nitrogen fertilization had three levels: not using nitrogen(N_0),using pure nitrogen of 195 kg/hm^2(N_(195)) and 255 kg/hm^2(N_(255)).Results showed that:(i)different supplemental irrigation and nitrogen fertilization significantly affected plant height and leaf area of winter wheat during key growth period.Under the same supplemental irrigation treatment,both plant height and leaf area of winter wheat showed as N_(255)> N_(195)> N_0(P <0.05).Plant height in N_(195) and N_(255)treatments was significantly higher than that in N_0 treatment,but there was not significant difference between N_(195) and N_(255)(P >0.05).Under the same nitrogen fertilization,plant height in W_2(569.4 m^3/hm^2) and W3(873.45 m^3/hm^2) treatments was significant higher than that in W_1(265.2 m^3/hm^2),but there was not significant difference between W_2 and W3(P >0.05).It illustrated that excessive nitrogen fertilization and supplemental irrigation did not significantly affect plant height and leaf area of winter wheat.(ii) Under the same nitrogen fertilization level,yield increase effect of winter wheat by supplemental irrigation showed a declining trend with nitrogen application amount increased.It illustrated that nitrogen fertilization and supplemental irrigation had certain critical values on the yield of winter wheat.When surpassing the critical value,the yield declined.When nitrogen fertilization amount was 195 kg/hm^2,and supplemental irrigation amount was 70% of field moisture capacity(569.4 m^3/hm^2),the highest yield 8500 kg/hm^2 could be obtained.(iii) During mature period of winter wheat,nitrogen accumulation amount of plant treated by nitrogen was significantly higher than that not treated by nitrogen(P <0.05).But under the treatments of W_2 and W3,nitrogen accumulation amount in N_(255) significantly declined when compared with N_(195)(P <0.05).Especially under W3(873.45 m^3/hm^2) level,nitrogen accumulation amount in N_(255) was even lower than N_0.Under the treatments of N_0 and N_(195),nitrogen accumulation amount of plant significantly increased with supplemental irrigation increased(P < 0.05).But under N_(255) treatment,there was not significant difference(P > 0.05).It illustrated that moderate supplemental irrigation and nitrogen fertilization could improve nitrogen absorption ability of winter wheat,but excessive supplemental irrigation and nitrogen fertilization were not favorable for plant's nitrogen absorption.(iv) Although the increase of supplemental irrigation during jointing period improved nitrogen absorption ability of winter wheat and promoted winter wheat absorbing more nitrogen,it inhibited nitrogen transferring and distributing to seed.Comprehensively considering growth condition of winter wheat and nitrogen risk condition,it is suggested that nitrogen application amount was 195 kg/hm^2,and supplemental irrigation reached 70% of field moisture capacity(569.4 m^3/hm^2),which could be as the suitable water and fertilizer use amounts in the region.
基金Supported by Special Fund for Innovation-driven Development in Guangxi Zhuangzu Autonomous Region(GK AA17204087-11)Natural Science Foundation of China(31560206 31760201)
文摘[Objectives]This study was conducted to reveal the characteristics of nutrient absorption and accumulation in Pinus massoniana plantations in Northwestern Guangxi.[Methods]Based on field investigation and indoor analysis,the contents,accumulation and annual net accumulation of five nutrient elements(N,P,K,Ca and Mg)in a mature P.massoniana plantation(26-year-old)in Nandan County,Guangxi Province were studied.[Results]The contents of nutrient elements in different organs of the mature P.massoniana plantation were the highest in the leaves,followed by the bark,branch and root,and the lowest in the stem.In general,among the contents of the five elements in different organs,N content was the highest,followed by K or Ca,and P and Mg were the lowest.The total accumulation of nutrient elements in the 26-year-old mature P.massoniana plantation in northwestern Guangxi was 1 384.05 kg/hm^2.Among the different structural levels of the stand,the tree layer had the highest accumulation of nutrient elements,which was 1 198.41 kg/hm^2,accounting for 86.59%of the total accumulation of nutrients in the plantation,and the accumulation of nutrients in other layers from the largest to the smallest was the litter layer(91.97 kg/hm^2),herb layer(49.86 kg/hm^2)and shrub layer(43.92 kg/hm^2),accounting for 3.17%,3.60%and 6.64%of the total nutrient accumulation of the plantation,respectively.The annual net accumulation of nutrient elements in the tree layer of the mature P.massoniana plantation was 46.09 kg/(hm^2·a),and the order of the annual net accumulation of different nutrient elements followed N>K>Ca>Mg>P;and the accumulation of 1 t of dry matter needed 6.37 kg of the five nutrients.[Conclusions]This study provides a scientific basis for the rational management of P.massoniana plantations,especially forest soil management.
基金financially the National Key Research and Development Program of China(2016YFD0300106,2018YFD0300603)the Shandong Modern Agricultural Technology&Industry System(SDAIT-02-08)。
文摘Planting maize at high densities leads to early leaf senescence,and the resulting reduction in the number of lower leaves affects the plant’s root function and lowers its grain yield.However,the nature of the process by which lower leaf senescence affects biomass accumulation and grain yield formation in maize is not clear.This study aimed to shed light on how these factors are related by investigating the effects of the plant growth regulator 6-benzyladenine(6-BA)on the senescence of lower leaves of maize plants.In two maize cultivars planted at densities of 67,500(low density,LD)and 90,000(high density,HD)plants ha^(-1),plants treated with 6-BA maintained a high green leaf area index(LAI)longer than control(CK)plants,enabling them to maintain a higher photosynthetic rate for a longer period of time and produce more biomass before reaching physiological maturity.Spraying the lower leaves of maize plants with a 6-BA solution increased the distribution of;C-photosynthates to their roots,lower leaves and bracts,a result that can be ascribed to a decreased retention of;C-photosynthates in the stem and grain.In both seasons of the experiment,maize plants treated with 6-BA accumulated more N in grain and maintained a higher N content in roots and leaves,especially in lower leaves,than CK.Increased C assimilation in the lower leaves may explain why N uptake in plants subjected to the 6-BA treatment exceeded that in CK plants and why both photosynthesis rate and dry matter accumulation were maintained throughout grain filling.Our results suggest that a suitable distribution of C and N in leaves post-silking may maintain plant root function,increase N use efficiency,maximize the duration of high LAI,and increase grain yield.
基金partially supported by Tianjin Marine Revitalization Plan of Science and Technology (No.KJXH2013-16) from Tianjin Oceanic Administration,China
文摘Microalgal lipids are regarded as main future feedstock of biofuels for its higher efficiency of accumulation and sus- tainable production. In order to investigate the effect of various nitrogen to phosphorus ratios on cells growth, chlorophyll content and accumulation of lipids in Dunaliella tertiolecta, experiments were carried out in modified microalgal medium with inorganic nitrogen (nitrate-nitrogen) or organic nitrogen (urea-nitrogen) as the sole nitrogen source at initial N:P ratios ranging from 1:1 to 32:1. The favorable N:P of 16:1 in the nitrate-N or urea-N medium yielded the maximum cell density and specific growth rate. Decrease in chlorophyll content were observed at the N:P of 4:1 in both nitrate-N and urea-N cultures. It was also observed that the maximum lipids concentration was obtained at the N:P of 4:1 in both nitrate and urea nutrient medium. The lipid productivity and lipid content of cultures in the urea-N medium at the N:P of 4: lwere markedly higher than those from cultures with other N:P ratios (p〈 0.05). The results of this work illustrate the possibility that higher ratios of nitrogen to phosphorus have enhancing effect on cells growth of D. tertiolecta. Conversely, higher lipid accumulation is associated with a decrease in chlorophyll content under lower ratios of nitro- gen to phosphorus. The results confirm the hypothesis of this study that a larger metabolic flux has been channeled to lipid accumu- lation in D. tertiolecta cells when the ratios of nitrogen to phosphorus drop below a critical level.
基金financially supported by the National Natural Science Foundation of China (31370425, 31501276)the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2015BAD22B01)
文摘The leaf photosynthesis and nitrogen(N) translocation in three large-spike lines and control cultivar(Xi'nong 979) of winter wheat(Triticum aestivum L.) were studied in 2010–2011 and 2011–2012. The objectives of this study were to investigate the differences in the physiological characteristics of large-spike lines and control cultivar and identify the limiting factors that play a role in improving the yield of breeding materials. The average yield, grain number per spike, kernel weight per spike, and 1 000-kernel weight of the large-spike lines were 16.0, 26.8, 42.6, and 15.4%, respectively, significantly higher than those of control. The average photosynthetic rates(Pn) were not significant between the large-spike lines and control cultivar during the active growth period. The average PSII maximum energy conversion efficiency(Fv/Fm), PSII actual quantum efficiency(Ф(PSII)), photochemical quenching coefficient(qP), PSII reaction center activity(Fv′/Fm′) and water-use efficiency(WUE) of the large-spike lines were 1.0, 5.1, 3.6, 0.8, and 43.4%, respectively, higher than those of the control during the active growth stages. The N distribution proportions in different tissues were ranked in the order of grains〉culms+sheathes〉rachis+glumes〉flag leaves〉penultimate leaves〉remain leaves. This study suggested that utilization of the large-spike wheat might be a promising approach to obtain higher grain yield in Northwest China.
基金funded by the Superior Cultivars Program of Shandong Province Government and Open Foundation Program of Chinese Academy of Sciences,China
文摘It is very important to study eco-physiological processes of plants and to determine quantitative relations between accumulation, distribution of dry matter and environmental factors for regionalization, standardization and precision agriculture. Meanwhile, global changes, e.g., atmospheric CO2 concentration rising, global warming, and climate abnormity, have been effecting on agricultural productivity. This study provides a theoretical basis for predicting productive potentials and development trends in different agricultural regions. One-year-old black walnut (Juglans hindsii) seedlings were employed as subjects for setting up the dynamic models of dry matter accumulation and distribution, based on mechanistic models of photosynthesis, matter conservation and concentration gradient. Under optimum conditions of soil moisture and mineral nutrient, during the period of the canopy construction, the dry matter accumulation of the canopy conformed to logistic curves; but the accumulation of both total biornass and dry matter of stem-root could be divided into two phases: the first phase was exponential increase, the second was linear increase. The total biomass, dry matter of canopy and stem-root all presented a fluctuant increase, which was affected by the environmental factors. Ratio of daily increase of dry matter in the canopy and the steem-root (dWJdWs) was changeable along with growth periods and environmental factors. At the initial stage of the canopy forming, dW/dWs was larger, about 3.2 on average, which indicated that the photosynthetic product was mainly used to develop leaves; in the midterm, it was about 1.9, which indicated that the distribution of dry matter in the canopy and in the stem-root was relatively balanced; when the plant tended to stop growing, dWl/dWs decreased linearly, and the main distribution of dry matter moved to the roots.
基金Supported by Special Promotion Plan of Innovation Team of Qinghai University(2014-NKY-209)&Special Project for Kunlun Scholars of Qinghai University&Special Project of Qinghai Provincial Key Laboratory(2015-Z-Y13)
文摘With Qingyu 1 and Qingyu 2 as test materials, variation of soluble carbohydrate content in different parts were studied under two types of artificially simulated drought stress (polyethylene glycd PEG-6000 stress and water-controlling stress). The results showed that under the stress of PEG, soluble carbohydrate content in leaves of Qingyu 1 and Qingyu 2 increased with the prolonging time of stress; soluble carbohydrate content in stems of Qingyu 1 did not show regular changes with the prolonging time of stress, while 30% PEG stress received the best effect on Qingyu 2 after 24 h; soluble carbohydrate content in roots of Qingyu 1 and Qingyu 2 increased with the prolonging time of stress. Under different intensities of water-controUing stress, soluble carbohydrate content in leaves of Qingyu 1 first increased then decreased, while that in leaves of Qingyu 2 increased; soluble carbohydrate content in stems of Qingyu 1 and Qingyu 2 increased with the pro- longing time of water-controlling stress; soluble carbohydrate content in roots of Qingyu 1 decreased with the prolonging time of water-contmlling stress, while that in roots of Qingyu 2 differed slightly.
基金Sponsored by 2015 Safety Production and Major Accident Prevention Program of State Administration of Work Safety(shandong-0017-2015AQ)
文摘To study the accumulation regularity of nitrogen and phosphorus in typical constructive plants in coastal wetland,samples of Suaeda glauca(Bunge) Bunge,Phragmites austrahs and Tamarix chinensis Lour,were taken from the Yellow River Delta National Coast Wetland Nature Reserve,nitrogen and phosphorus content in plants was measured and analyzed.The results showed that ① nitrogen and phosphorus content in different wetland plants is correlated;② different species in the same place and the same species in different spaces show different accumulation regularity of nitrogen and phosphorus;③ nitrogen and phosphorus content in plants is closely related to nitrogen and phosphorus content in the habitat;④ nitrogen content in T.chinensis Lour,is the highest,the mean is 11.63 g/kg,and phosphorus content in S glauca(Bunge) Bunge is the highest,the mean is 1.38 g/kg;⑤ nitrogen content in the 3 species:T.chinensis Lour.> S.glauca(Bunge) Bunge > P.australis;⑥ nitrogen content in aboveground parts of all plants is significantly higher than that in underground parts,and phosphorus content in aboveground parts of all plants except S.glauca(Bunge) Bunge is significantly higher than that in underground parts;⑦ nitrogen content in the 3 species in the study area is significantly higher than phosphorus content in these species.
基金This research was financially supported by the National Key Research and Development Project(2018YFD20030503)of China.
文摘Readily available chemical fertilizers have resulted in a decline in the use of organic manure(e.g.,green manures),a traditionally sustainable source of nutrients.Based on this,we applied urea at the rate of 270 kg ha−1 with and without green manure in order to assess nitrogen(N)productivity in a double rice cropping system in 2017.In particular,treatment combinations were as follows:winter fallow rice-rice(WF-R-R),milk vetch rice-rice(MV-R-R),oil-seed rape rice-rice(R-R-R)and potato crop rice-rice(P-R-R).Results revealed that green manure significantly(p≤0.05)improved the soil chemical properties and net soil organic carbon content increased by an average 117.47%,total nitrogen(N)by 28.41%,available N by 26.64%,total phosphorus(P)by 37.77%,available P by 20.48%and available potassium(K)by 33.10%than WF-R-R,however pH was reduced by 3.30%across the seasons.Similarly,net dry matter accumulation rate enhanced in green manure applied treatments and ranked in order:P-R-R>R-R-R>MV-R-R>WF-R-R.Furthermore,the total leaf dry matter transport(t ha−1)for the P-R-R in both seasons was significantly higher by an average 11.2%,7.2%and 36%than MV-R-R,R-R-R,and WF-R-R,respectively.In addition,net total nitrogen accumulation(kg ha−1)was found higher in green manure applied plots compared to the control.Yield and yield attributed traits were observed maximum in green manure applied plots,with treatments ranking as follows:P-R-R>R-R-R>MV-R-R>WF-R-R.Thus,results obtained highlight ability of green manure to sustainably improve soil quality and rice yield.