Macrophages have recently been characterized as having an M1 or M2 phenotype based on receptor expression, mechanism of activation and function. The effects of macrophage phenotype upon tissue remodeling following imp...Macrophages have recently been characterized as having an M1 or M2 phenotype based on receptor expression, mechanism of activation and function. The effects of macrophage phenotype upon tissue remodeling following implantation of an acellular dermal matrix (ADM) is largely unknown. The purpose of this study was to compare the macrophage phenotype and tissue remodeling elicited by four different ADMs (DermaMatrix, AlloDerm, Integra and Der mACELL). ADM samples were wrapped around the inferior epigastric vessels of a rat and were harvested on 7, 14, 21 and 42 days post implantation. Immunohistologic methods were used to identify macrophage surface markers CD68 (pan macrophage), CCR7 (M1 profile), and CD206 (M2 profile). All human derived ADMs showed a bell shaped curve for distribution of CD68+ macrophages with peaks for DermaMatrix occurring at day 14 and peak influx for AlloDerm occurring on day 21. In contrast, bovine derived Integra showed an increasing trend of macrophages with time. DermACELL had the highest influx of macro- phages while Integra had the lowest. A quantitative analysis of phenotype of macrophages in AlloDerm showed that the cells were predominantly M1 at 7, 14, 21 and 42 days post implantation. In contrast, Integra showed a mixed M1/M2 population of macrophages at all time points. The histopathologic evaluation showed that a predominantly M1 macrophage response was associated with a more inflamematory type tissue remodeling outcome in AlloDerm while a mixed M1/M2 macrophage response was associated with a more constructive tissue remodeling response seen in the other substrates.展开更多
PC-3000 for Windows作为俄罗斯ACELAB公司推出的商用硬盘修复工具,是一项对受损硬盘驱动进行数据恢复的技术方案。它专为小型商业开发,不需要使用无尘室,硬盘复写器或其他昂贵设备。该软件具有人性化设计的硬盘驱动器固件信息数据库,...PC-3000 for Windows作为俄罗斯ACELAB公司推出的商用硬盘修复工具,是一项对受损硬盘驱动进行数据恢复的技术方案。它专为小型商业开发,不需要使用无尘室,硬盘复写器或其他昂贵设备。该软件具有人性化设计的硬盘驱动器固件信息数据库,允许维修人员在数据库快速搜索硬盘驱动器资源,避免了必须花费大量精力查找匹配固件的毛病。展开更多
文摘Macrophages have recently been characterized as having an M1 or M2 phenotype based on receptor expression, mechanism of activation and function. The effects of macrophage phenotype upon tissue remodeling following implantation of an acellular dermal matrix (ADM) is largely unknown. The purpose of this study was to compare the macrophage phenotype and tissue remodeling elicited by four different ADMs (DermaMatrix, AlloDerm, Integra and Der mACELL). ADM samples were wrapped around the inferior epigastric vessels of a rat and were harvested on 7, 14, 21 and 42 days post implantation. Immunohistologic methods were used to identify macrophage surface markers CD68 (pan macrophage), CCR7 (M1 profile), and CD206 (M2 profile). All human derived ADMs showed a bell shaped curve for distribution of CD68+ macrophages with peaks for DermaMatrix occurring at day 14 and peak influx for AlloDerm occurring on day 21. In contrast, bovine derived Integra showed an increasing trend of macrophages with time. DermACELL had the highest influx of macro- phages while Integra had the lowest. A quantitative analysis of phenotype of macrophages in AlloDerm showed that the cells were predominantly M1 at 7, 14, 21 and 42 days post implantation. In contrast, Integra showed a mixed M1/M2 population of macrophages at all time points. The histopathologic evaluation showed that a predominantly M1 macrophage response was associated with a more inflamematory type tissue remodeling outcome in AlloDerm while a mixed M1/M2 macrophage response was associated with a more constructive tissue remodeling response seen in the other substrates.
文摘PC-3000 for Windows作为俄罗斯ACELAB公司推出的商用硬盘修复工具,是一项对受损硬盘驱动进行数据恢复的技术方案。它专为小型商业开发,不需要使用无尘室,硬盘复写器或其他昂贵设备。该软件具有人性化设计的硬盘驱动器固件信息数据库,允许维修人员在数据库快速搜索硬盘驱动器资源,避免了必须花费大量精力查找匹配固件的毛病。
