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南极真菌青霉(Penicillium sp.)S-3-88摇瓶发酵条件的初步优化
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作者 方莎莎 唐潮 +3 位作者 叶科元 卢小玲 刘小宇 焦炳华 《中国抗生素杂志》 CAS CSCD 北大核心 2016年第2期109-112,共4页
目的对南极来源的真菌青霉Penicillium sp.S-3-88进行细胞毒活性筛选及发酵条件优化。方法对菌株进行小量发酵及代谢产物提取,检测其粗提物对6种肿瘤细胞株的抑制活性;通过对培养基、培养温度、摇床转速和发酵时间的筛选,以细胞毒活性... 目的对南极来源的真菌青霉Penicillium sp.S-3-88进行细胞毒活性筛选及发酵条件优化。方法对菌株进行小量发酵及代谢产物提取,检测其粗提物对6种肿瘤细胞株的抑制活性;通过对培养基、培养温度、摇床转速和发酵时间的筛选,以细胞毒活性和次级代谢产物产量为指标对该菌株的培养条件进行初步优化。结果 Penicillium sp.S-3-88对6种肿瘤细胞株具有不同程度的抑制活性,其中对MCF-7和SGC-7901细胞的抑制率最高,分别达到78.79%和74.66%(初提物浓度为50μg/m L)。初步优化后,优选的发酵条件为:PDB培养基,温度20℃,发酵时间14d,转速180r/min。在此条件下进行发酵,Penicillium sp.S-3-88的次级代谢产物量和细胞毒活性分别比优化前高203.57%、48.32%(A549)和44.92%(SW-1990)。结论获得了一株具有强细胞毒活性的真菌Penicillium sp.S-3-88,优化了该菌株的发酵条件,有助于后期的活性次级代谢产物的分离纯化。 展开更多
关键词 南极真菌 PENICILLIUM sp.s-3-88 细胞毒活性 发酵条件优化
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Removal of sulfamethazine antibiotics by aerobic sludge and an isolated Achromobacter sp.S-3 被引量:12
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作者 Manhong Huang Shixuan Tian +3 位作者 Donghui Chen Wei Zhang Jun Wu Liang Chen 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2012年第9期1594-1599,共6页
Removal characteristics of sulfamethazine (SMZ) by sludge and a bacterial strain using an aerobic sequence batch reactor (ASBR) were studied. Operating conditions were optimized by varying the reaction time and sl... Removal characteristics of sulfamethazine (SMZ) by sludge and a bacterial strain using an aerobic sequence batch reactor (ASBR) were studied. Operating conditions were optimized by varying the reaction time and sludge retention time (SRT). AnAchromobacter sp. (S-3) with the ability to remove SMZ was isolated from the ASBR. The effects of different operating parameters (pH and temperature) on the biodegradation of SMZ by S-3 were determined. The results indicate that, between 0.5 and 4 hr, reaction time of the ASBR had a significant effect on the SMZ removal efficiency in the system. The SMZ removal efficiency also increased from 45% to 80% when SRT was prolonged from 5 to 25 days, although longer SRT had no impact on SMZ removal. The SMZ adsorption rate decreased with increasing temperature, which fitted Freundiich isotherm well. The removal of SMZ in the ASBR was due to the combined effects of adsorption and degradation, and degradation played a leading role. 展开更多
关键词 aerobic sludge achromobacter sp. s-3 BIODEGRADATION SULFAMETHAZINE
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