Phytoremediation of Oil-Contaminated Soils by Combining Flowering Plant Cultivation and Inoculation with <i>Acinetobacter junii</i>Strain M-2

Oil contamination of the soil by petroleum products has become an enormous environmental problem. In this study, we examined whether remediation of oil-contaminated soils by cultivating three flowering plants (Mimosa, Gazania, and Zinnia) could be enhanced by inoculation with Acinetobacter junii strain M-2 at different plant growth stages (at sowing, at early growth, and at mid-growth). The growth of Zinnia cultivated in oil-contaminated soils inoculated at sowing was significantly superior to that in the non-inoculated soil. Although total petroleum hydrocarbon concentrations in soils inoculated at sowing were nominally lower than those in non-inoculated soils, especially in the case of Zinnia planting, the effect did not reach statistical significance. However, dehydrogenase activity was significantly higher in the soils inoculated with A. junii strain M-2 than in non-inoculated soils for all three plant species tested. These results demonstrate that a combination of ornamental plant cultivation (particularly Zinnia) and inoculation with A. junii strain M-2 increases the efficiency of oil-contaminated soil phytoremediation.

一株耐高氨氮菌株的筛选及脱氮性能研究

硝化反硝化生物脱氮被认为是目前有效的氨氮废水处理技术之一,其关键是高效脱氮微生物的筛选。本研究从养猪厂污水处理池中筛选异养硝化好氧反硝化细菌,并探究其生长性能和脱氮性能。结果筛选出一株异养硝化好氧反硝化细菌Acinetobacter junii YQ-1,当温度为30℃、转速为200 r/min、初始pH值为8、接种量为2.0%(V/V)、培养时间为48 h时,菌株YQ-1具有最高的菌体密度(OD_(595)=0.999),且脱氮效果最好。在最佳生长条件下,当初始氨氮浓度、亚硝态氮和硝态氮浓度均为200 mg/L时,菌株YQ-1在48 h对这3种形式氮素的降解率分别达到89.73%、63.51%和70.19%。且当氨氮浓度为1000 mg/L时,菌株YQ-1仍能较好地生长,其生长情况符合BidoseResp模型,培养基中化学需氧量(Chemical Oxygen Demand,COD)变化情况符合ExpDec2模型,培养基中化学需氧量(Chemical Oxygen Demand,COD)的减少速率与菌株YQ-1的生长速率呈正相关关系。本研究为异养硝化好氧反硝化菌处理高浓度氨氮污水的实际应用提供了参考。