Characterization of hematopoiesis in Dabry's sturgeon(Acipenser dabryanus)

The hematological indices of Dabry's sturgeon(Acipenser dabryanus)were investigated in this paper.The results showed that the count of Red blood cell(RBC)was(0.60±0.02)
1012/L,and the blood glucose(GLU)was 9.32±1.00 mmol/L.Glutamic-pyruvic transaminase(ALT)and glutamic-oxalacetic transaminase(AST)were 13.75±6.65 U/L and 359.50±40.07 U/L,respectively.Smears of peripheral blood and imprints of four tissues were studied in the trial with Wright's staining method.The results indicated that Dabry's sturgeon's blood cells could be divided into five cell types,and included erythroid,granuloid,monocytoid,lymphoid and other cells.In addition,the developmental stage of each cell lineage could be subdivided into:primitive,young and mature.In the three developmental stages,the size of each cell type was similar and gradually decreased from primitive,young to mature blood cells.Among the blood cells,the monoblast and macrophage were the biggest,while the thrombocyte was the smallest.All stages of the primitive cells and young cells were observed in head kidney and all cell types,except granuloblasts,were identified in the head kidney.In contrast,primitive cells and young cells were not observed in the spleen or liver,and few orthochromatic erythroblasts were found in peripheral blood.It can be concluded that head kidney and trunk kidney are the primary organs of hematopoiesis in the Dabry's sturgeon.

饲料锌对长江鲟幼鱼肠道微生物组成的影响

为了解锌对长江鲟(Acipenser dabryanus)类肠道菌群的多样性及群落结构组成的影响,本研究配制了14.77,57.71和364.5 mg/kg的3种不同锌水平的饲料,养殖长江鲟幼鱼8周,采集了后肠粪便,测定了后肠粪便的肠道α-多样性、群落结构组成与OTU数量。结果显示:随饲料锌水平的升高,基于丰度的覆盖估计值(ACE指数)、Observed species指数、Chao1丰富度估计指数(Chao1指数)出现了下降的趋势,364.5 mg/kg组最低,364.5 mg/kg组与14.77 mg/kg组之间差异显著,与57.71mg/kg组之间无显著差异。香浓多样性指数、辛普森多样性指数与普系α-多样性(Phylogenetic diversity,PD-whole tree)值也表现出随饲料锌水平的升高而下降的趋势,且三种指数均在364.5 mg/kg时最低,但各组之间无显著性差异。在科水平上,梭杆菌科、肠杆菌科与梭菌科类的总和分别占前10种科的80.82%、92.89%、93.37%,表明在不同锌饲料处理水平下,上述3个菌科在鱼类肠道菌群的组成中占据优势。在属水平上,鲸杆菌属(Cetobacterium)、梭菌属类(unidentified_Clostridiales)、邻单胞菌属(Plesiomonas)与柠檬酸杆菌属(Citrobacter)总和分别占前10种属的总和百分比分别达78.32%、81.56%、89.28%,表明这4种细菌属是优势属。三个处理组的总OTU数随饲料锌水平的升高而出现了下降的趋势,三个处理组的OTU数分别为1160、790、654。上述结果表明,饲料锌含量对长江鲟幼鱼肠道的α-多样性、群落结构组成及OTU数量产生了影响,且饲料锌含量过高时会影响长江鲟幼鱼肠道的α-多样性、群落结构丰度及OTU数。